Scribd Logo
Upload

Welcome to Scribd!

  • 176 views

    Bio 175 Lab Test I Study Guide

    Uploaded by

    Marco Calvara
    This document provides a study guide for a microbiology lab test, listing questions about microscopy, staining techniques, bacterial culture and identification. It asks about the proper use and care of microscopes, Gram staining procedure and results, culture methods like streak plating and aseptic transfers, and identification of bacteria used in the lab like Serratia marcescens. Safety and cleaning procedures for the lab are also addressed.

    Copyright:

    Attribution Non-Commercial (BY-NC)
    Download as DOC, PDF, TXT or read online from Scribd

    Bio 175 Lab Test I Study Guide

    Uploaded by

    Marco Calvara
    176 views2 pages
    This document provides a study guide for a microbiology lab test, listing questions about microscopy, staining techniques, bacterial culture and identification. It asks about the proper use and care of microscopes, Gram staining procedure and results, culture methods like streak plating and aseptic transfers, and identification of bacteria used in the lab like Serratia marcescens. Safety and cleaning procedures for the lab are also addressed.

    Original Title

    Bio 175 Lab Test i Study Guide

    Copyright

    © Attribution Non-Commercial (BY-NC)

    Available Formats

    DOC, PDF, TXT or read online from Scribd

    Did you find this document useful?

    Is this content inappropriate?

    This document provides a study guide for a microbiology lab test, listing questions about microscopy, staining techniques, bacterial culture and identification. It asks about the proper use and care of microscopes, Gram staining procedure and results, culture methods like streak plating and aseptic transfers, and identification of bacteria used in the lab like Serratia marcescens. Safety and cleaning procedures for the lab are also addressed.

    Copyright:

    Attribution Non-Commercial (BY-NC)
    Download as DOC, PDF, TXT or read online from Scribd
    Download as doc, pdf, or txt
    176 views2 pages

    Bio 175 Lab Test I Study Guide

    Uploaded by

    Marco Calvara
    This document provides a study guide for a microbiology lab test, listing questions about microscopy, staining techniques, bacterial culture and identification. It asks about the proper use and care of microscopes, Gram staining procedure and results, culture methods like streak plating and aseptic transfers, and identification of bacteria used in the lab like Serratia marcescens. Safety and cleaning procedures for the lab are also addressed.

    Copyright:

    Attribution Non-Commercial (BY-NC)
    Download as DOC, PDF, TXT or read online from Scribd
    Download as doc, pdf, or txt
    You are on page 1of 2

    BIO 175 LAB TEST I – STUDY GUIDE

    What type of microscope is used in lab?____________________________________


    How do you properly carry a microscope. _____________________________________
    What is used to properly clean microscope lenses in OUR lab? _____________________
    What objective is used most by a microbiologist? _______________________________
    Identify parts of the microscope
    Which adjustment knob is used to sharpen the image on high – dry examination?
    Define parfocal
    Why is immersion oil used on the 100X objective lens.
    If you are getting 400 X magnification with a 40 X high dry objective, what is the power
    of the ocular?
    Laboratory microscope oculars have the magnification of
    The total magnification of an image with the low power objective and the 10 X oculars is
    Describe step-by-step the preparation of a smear from a liquid media.
    Name reasons for heat-fixing the slide after the smear is air-dried.
    What is the disinfectant used in our lab ?_________________________________
    In making an aseptic transfer what is the FIRST STEP in the transfer?
    How do you prepare a slide before transferring the microbe to the slide?
    Why is it best to hold a slide between your fingers when passing the slide through a
    Bunsen burner flame to heat-fix the slide
    In the handling culture lab what microbe was used._______________________________
    How did the bacterium appear when growing in broth or on slant?
    At what temperature must S. marcescens be grown for pigmentation to occur.
    The Gram stain separates bacteria into how many bacterial groups?
    Why do Gram positive bacteria retain the crystal violet dye?
    Which dye stains the gram positive bacteria.____________________________________
    Which dye stains the gram negative bacterium. _________________________________
    What is a mordant.
    Give an example of a mordant.________________________________
    What is the decolorizing agent used in lab?___________________________________
    What effect does aging have on a bacterial culture and Gram staining?
    Name microbes that was gram positive in lab . _________________________________
    Name microbes in lab that was gram negative. ________________________________
    Name a genus of bacteria that are endospore formers. __________________________
    Name something that an endospore is resistant to______________________________
    What is done first and last to your lab table each time?
    What is a pure culture?
    How do most microorganisms exist in the environment?
    Name microbes used in the Mixed Broth.
    What is the name of the machine used to stir broth media?
    Why is it necessary to isolate individual colonies from a mixed culture?
    Describe an aseptic transfer step–by-step.
    Describe the streak dilution technique used in lab step-by-step.

    You might also like