Physical Evidence Manual (3940 - 2)

PHYSICAL EVIDENCE MANUAL
OREGON STATE POLICE

FORENSIC SERVICES DIVISION
OREGON STATE POLICE FORENSIC SERVICES DIVISION
PHYSICAL EVIDENCE M ANUAL

PREFACE
PREFACE
The purpose of this manual is to educate our customers in the Criminal Justice System regarding the
services provided by the Oregon State Police Forensic Services Division, and the recommended
methods of documenting, collecting, and preserving physical evidence to ensure the best analysis
results.
Every attempt has been made to make this handbook as current and up-to-date as possible; however, it
should be noted that the field of forensic science is changing at a rapid rate. New techniques and
procedures are constantly being developed, providing new capabilities or refinements to existing
capabilities.
Consequently, techniques, procedures and capabilities contained herein may change in the future. All
agencies are encouraged to keep in regular contact with their local laboratory to keep abreast of any
such changes. This handbook will be stored on the State Police Internet site:
http://www.oregon.gov/osp/FORENSICS/Pages/OSP%20FORENSICS.aspx
Issuing Authority: Effective:

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Operations Manager September 10, 2015
ALL COPIES OUTSIDE OF POLICYTECH ARE UNCONTROLLED Page 2 of 94

TABLE OF CONTENTS
1.0 Forensic Services Division Mission Statement ................................................................................... 8

2.0 Introduction ........................................................................................................................................ 9
3.0 Laboratory Locations and Services .................................................................................................. 12
4.0 Case Communication ....................................................................................................................... 14
5.0 General Evidence Handling.............................................................................................................. 15
5.1 General Evidence Handling Guidelines ..................................................................................... 15
5.2 Evidence Collection .................................................................................................................. 15
5.3 Chain of Custody ...................................................................................................................... 17
5.4 Forensic Services Request Form .............................................................................................. 18
6.0 Laboratory Reports .......................................................................................................................... 21
6.1 Laboratory Online Information System (LOIS) ........................................................................... 21
6.2 Forensic Laboratory Evaluation Form and Surveys ................................................................... 21
7.0 Field Investigations (Crime Scenes) ................................................................................................. 22
7.1 Crime Scene Services Offered .................................................................................................. 22
7.2 Processing a Crime Scene ........................................................................................................ 23
7.3 Notes ........................................................................................................................................ 23
7.4 Photography ............................................................................................................................. 23
7.5 Diagrams/Sketches ................................................................................................................... 23
7.6 Conduct a Detailed Search ....................................................................................................... 24
7.7 Vehicle Examinations ................................................................................................................ 24
7.8 Body Processing ....................................................................................................................... 25
7.9 Postmortem Examinations ........................................................................................................ 25
7.10 Decomposed Remains ............................................................................................................ 26
7.11 Bloodstain Pattern Analysis..................................................................................................... 26
8.0 Missing Persons (‘MP’) .................................................................................................................... 27
8.1 Secondary Standards or Direct Reference Sample ................................................................... 27
8.2 DNA from the Missing Person’s Relatives (Family Reference Standards) ................................. 28

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TABLE OF CONTENTS
8.3 Family Reference Standard Collection Kit ................................................................................. 28

9.0 Unidentified Remains ....................................................................................................................... 29
9.1 Unidentified Remains ................................................................................................................ 29
9.2 Identifying the Deceased ........................................................................................................... 29
10.0 Biological Evidence ........................................................................................................................ 31
10.1 Safety and Contamination Prevention ..................................................................................... 31
10.2 General Collection Guidelines ................................................................................................. 31
10.3 Alternate Light Source Searches ............................................................................................. 32
10.4 Blood Evidence ....................................................................................................................... 33
10.5 Saliva Evidence ...................................................................................................................... 34
10.6 Semen Evidence ..................................................................................................................... 35
10.7 Adult and Child Sexual Assault Forensic Evidence (SAFE-A and SAFE-C) Kits...................... 35
10.8 Penile Swabbing Forensic Evidence (PSFE) Kits .................................................................... 36
11.0 DNA Analysis ................................................................................................................................. 38
11.1 Body Fluid Analysis ................................................................................................................. 38
11.2 Wearer DNA............................................................................................................................ 39
11.3 Aggressive Handling DNA ....................................................................................................... 39
11.4 Touch DNA ............................................................................................................................. 39
11.5 Criminal Paternity .................................................................................................................... 40
11.6 Y-STR Analysis ....................................................................................................................... 40
11.7 DNA Standards ....................................................................................................................... 40
11.8 Decomposed Remains ............................................................................................................ 41
11.9 Secondary Standards.............................................................................................................. 42
11.10 No Available Suspect Standards ........................................................................................... 42
11.11 High Throughput Property Crimes Program .......................................................................... 42
11.12 Private Laboratory Analysis ................................................................................................... 42
12.0 Latent Print Evidence ..................................................................................................................... 43

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TABLE OF CONTENTS
12.1 Collection, Packaging, and Submission of Evidence ............................................................... 43

12.2 Submission of Inked Prints for Comparison Purposes ............................................................. 45
12.3 Automated Biometric Identification System (ABIS) .................................................................. 45
12.4 Identifying the Deceased ......................................................................................................... 45
13.0 Drug Chemistry .............................................................................................................................. 47
13.1 Analysis of controlled substances ........................................................................................... 47
13.2 Submission Policies ................................................................................................................ 47
13.3 Collection and Packaging ........................................................................................................ 49
13.4 LSD......................................................................................................................................... 49
13.6 Latent Prints ............................................................................................................................ 49
13.7 Field Test Kits ......................................................................................................................... 49
13.8 Clandestine Drug Laboratories ................................................................................................ 50
13.9 Methamphetamine Quantification ............................................................................................ 51
14.0 Anonymous Analysis Program Of Suspected Controlled Substances ............................................ 52
14.1 Submission of Substances for Anonymous Analysis ............................................................... 52
15.0 Toxicology ...................................................................................................................................... 53
15.1 Type of Sample ....................................................................................................................... 53
15.2 Triaging ................................................................................................................................... 53
15.3 Blood Alcohol .......................................................................................................................... 54
15.4 Urine ....................................................................................................................................... 54
16.0 Firearms Evidence ......................................................................................................................... 57
16.1 Integrated Ballistics Identification System (IBIS) ..................................................................... 57
16.2 Collection and Packaging of Firearms Evidence ..................................................................... 58
17.0 Serial Number Restoration ............................................................................................................. 60
17.1 Collection and Packaging of Evidence .................................................................................... 60
18.0 Tool Mark Evidence ....................................................................................................................... 61
18.1 Collection and Packaging of Evidence .................................................................................... 61

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TABLE OF CONTENTS
19.0 Gunpowder and Shot/Pellet Patterns ............................................................................................. 62

19.1 Collection of Gunpowder Evidence ......................................................................................... 62
20.0 Arson and Fire Debris ................................................................................................................... 63
20.1 Arson Scene Indicators ........................................................................................................... 63
20.2 Collection of Evidence............................................................................................................. 63
20.3 Packaging of Evidence............................................................................................................ 64
21.0 Explosives ...................................................................................................................................... 65
22.0 Glass Evidence .............................................................................................................................. 66
22.1 Collection and Packaging of Glass Standards ......................................................................... 66
22.2 Collection and Packaging of Recovered Glass Evidence ........................................................ 67
22.3 Clothing Items and Hair Combings .......................................................................................... 67
22.4 Vacuum Sweepings / Large Objects ....................................................................................... 67
23.0 Paint Evidence ............................................................................................................................... 68
23.1 Collecting and Packaging Paint Evidence ............................................................................... 68
24.0 Plastic and Tape Evidence ............................................................................................................ 73
24.1 Collection of Plastic Evidence ................................................................................................. 73
25.0 Miscellaneous Trace Evidence (Including Poison) ......................................................................... 74
26.0 Fiber Evidence ............................................................................................................................... 75
26.1 Fiber Examinations ................................................................................................................. 75
26.2 Collection of Fiber Evidence .................................................................................................... 76
26.3 Collection of Fiber Standards .................................................................................................. 76
26.4 Fabric Damage ....................................................................................................................... 77
27.0 Hair Evidence................................................................................................................................. 78
27.1 Hair Examinations ................................................................................................................... 78
27.2 DNA Analysis of Hairs ............................................................................................................. 78
27.3 Collection of Hair Evidence ..................................................................................................... 79
27.4 Collection of Hair Standards.................................................................................................... 80

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TABLE OF CONTENTS
28.0 Impression Evidence ...................................................................................................................... 82

28.1 Impression Evidence General Guidelines................................................................................ 82
28.2 Photographing Impression Evidence ....................................................................................... 82
28.3 Three-Dimensional Impressions .............................................................................................. 84
28.4 Casting Methods ..................................................................................................................... 84
28.5 Two Dimensional Impressions/Prints ...................................................................................... 85
28.6 Collection and Packaging ........................................................................................................ 86
28.7 Exemplars and Standards ....................................................................................................... 86
28.8 SICAR submission instructions for emailed images................................................................. 86
28.9 Tire Exemplars and Standard.................................................................................................. 90
29.0 Computer Evidence........................................................................................................................ 91
30.0 How to Make a Paperfold ............................................................................................................... 92
31.0 Manual Revision History ................................................................................................................ 93

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1.0 FORENSIC SERVICES DIVISION MISSION STATEMENT
1.0 FORENSIC SERVICES DIVISION MISSION STATEMENT

MISSION STATEMENT
The mission of the Department of Oregon State Police is to enhance livability and safety by protecting the
people, property and natural resources of the state.
The purpose of the Forensic Services Division (FSD) is to provide timely and accurate scientific, technical,
and investigative support to the criminal justice system through forensic analysis.
GOALS AND OBJECTIVES

The goals and objectives are based upon the needs of the Criminal Justice System and the agencies
served by the Forensic Services Division laboratories.
FORENSIC SERVICES DIVISION GOALS

 Provide timely, uniform delivery of services
 Match services with customer expectations
 Continue to provide a reliable, scientifically sound, high quality work product
 Maintain a highly qualified, motivated staff
 Strive for effective communication within the Division
 Make every effort to obtain adequate resources (monies, equipment, facilities and staffing) and
optimize their use to achieve its goals and objectives

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2.0 INTRODUCTION
2.0 INTRODUCTION
OSP has five laboratories across the state located in Bend, Central Point, Clackamas (Portland Metro),
Pendleton and Springfield. The labs provide services in the scientific examination of physical evidence,
collection and preservation of evidence, reconstruction of major crime scenes and expert testimony
regarding the scientific examinations. In addition, the FSD manages and administers the CODIS database
and the State of Oregon’s breath-testing instruments and officer certification.
All five OSP laboratories are accredited to the ISO/IEC 17025:2005 standard and ASCLD-LAB
Supplemental Requirements for Testing Laboratories. Additionally the DNA Unit is accredited to the FBI
Quality Assurance Standards (FBI QAS) for casework and database analysis.
The services of the laboratories are available to all local, state, and federal law enforcement agencies in
Oregon for the purpose of rendering assistance in criminal investigations and judicial proceedings.
Casework will also be conducted for the defense upon court order. Normally, all laboratory examinations,
court appearances, and travel expenses are available without charge.
The following is a list of the disciplines and a brief description of the type of forensic services provided.
BIOLOGY PROCESSING
The screening and preliminary processing of physical evidence for biological material (e.g., blood,
semen, saliva).
BREATH ALCOHOL PROGRAM (IMPLIED CONSENT UNIT)

The Implied Consent Unit services Oregon’s Breath Alcohol Program. The Unit approves breath
alcohol instruments, certifies the instruments, provides instrumentation, training and certification for
the users and offers expert testimony. The services of this unit are not accredited.
CONTROLLED SUBSTANCES
Qualitative analysis of physical evidence to determine if a controlled substance is present. Also
includes the analysis of evidence collected from suspected clandestine laboratory scenes to
determine the identification of chemicals and processes used to manufacture controlled substances.
Quantitative analysis may be performed on an as needed basis for methamphetamine cases only.
CRIME SCENE/FIELD INVESTIGATIONS

The analysis of physical locations or objects suspected to be involved in a crime. Analysis may
include recognition, documentation, collection and preservation of evidence and/or performing
scientific analysis, interpretation, and reconstruction.

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2.0 INTRODUCTION
DNA ANALYSIS
The analysis of biological evidence for the presence of autosomal-STR & Y-STR DNA. When an
interpretable DNA profile is developed, it can be compared to relevant standards and/or searched in
the CODIS database. DNA analysis is accomplished using the polymerase chain reaction and
capillary electrophoresis to examine short tandem repeats. The DNA unit also manages and
administers the State’s CODIS database.
FIREARMS/TOOLMARKS
Screening and comparisons of bullets and cartridge cases, firearm functionality, caliber determination
of cartridge cases and projectiles, proximity determination tests and restoration of obliterated
markings. Tool mark analysis compares marks left during the commission of a crime to test marks
from a tool possibly used in the crime. The IBIS database is used for the correlation of fired cartridge
cases to other entered cartridge case evidence in an attempt to provide links to previously unknown
related crimes.
LATENT PRINTS
Latent print processing is the physical and chemical processing of evidentiary items to develop and
preserve friction ridge detail. Friction ridge detail developed on evidentiary items is subsequently
compared to a person or persons or interest. The Western Identification Network (WIN) Automated
Biometric Identification System (ABIS) and the FBI’s Next Generation Identification (NGI) databases
are composed of known fingerprint standards and can be utilized to search latent prints of unknown
origin.
TOXICOLOGY
The qualitative analysis of biological fluids (e.g., urine) for controlled substances, common
pharmaceuticals and poisons. Quantitative analysis of drugs in blood is performed on postmortem
toxicology cases. Volatiles analysis (e.g., blood alcohol determination) is provided for ante-mortem
and post-mortem cases.
TRACE EVIDENCE
The screening for and the analysis of ignitable liquids, explosives, fibers, glass, paint, hair, footwear,
tire impressions and miscellaneous evidence.

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2.0 INTRODUCTION
OFFICE PHONE EMAIL

Bend Forensic Laboratory Phone: 541-388-6150 [email protected]
20355 Poe Sholes Drive Fax: 541-633-2323
Suite 200
Bend, OR 97703
Central Point Forensic Laboratory Phone: 541-776-6118 [email protected]
4500 Rogue Valley Highway Fax: 541-664-8742
Suite B
Central Point, OR 97502
Pendleton Laboratory Phone: 541-276-1816 [email protected]
405 SE 8th Street Fax: 541-278-1725
Pendleton, OR 97801
Portland Metropolitan Forensic Laboratory Phone: 971-673-8230 [email protected]
13309 SE 84th Avenue Fax: 971-673-8309
Suite 200
Clackamas, OR 97015
Springfield Forensic Laboratory Phone: 541-726-2590 [email protected]
3620 Gateway Street Fax: 541-726-2524
Springfield, OR 97477

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3.0 LABORATORY LOCATIONS AND SERVICES

Figure 1: Service Areas for the Oregon State Police, Forensic Services Division Laboratories

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The following is a list of services provided in each laboratory in the Forensic Services Division; however,
evidence maybe moved from one lab to another in order to complete the work more efficiently.
Portland Metro Lab Central Point Lab Pendleton Lab

Biology Processing Biology Processing Biology Processing
Chemistry Chemistry Chemistry
 Controlled  Controlled Substances  Controlled Substances
Substances  Clandestine Lab Analysis Field Investigations
 Clandestine Lab Field Investigations Firearms Processing
Analysis Firearms Processing Latent Print Processing
 Meth. Quant Latent Print Analysis Serial Number Restoration
DNA Serial Number Restoration Trace Processing
 DNA analysis Trace Processing
 Y-STR analysis Springfield Lab
 CODIS Bend Lab Biology Processing
Field Investigations Biology Processing Chemistry
Firearms/Tool mark Chemistry  Controlled Substances
analysis  Controlled Substances  Clandestine Lab
Implied Consent Program Field Investigations Analysis
Toxicology Latent Print Analysis  Meth. Quant
 Post-mortem  Inhalants
 Ante-mortem Crime Scenes Field
 Blood Alcohol Investigations
analysis Firearms/Tool mark analysis
Latent Print Analysis Latent Print Analysis
Trace Evidence analysis Toxicology
 Arson  Ante-mortem
 Glass  Blood Alcohol analysis
 Fibers Trace Evidence analysis
 Explosives  Impressions
 Impressions  Hairs
 Hairs  Paint
 Paint
 Miscellaneous

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4.0 CASE COMMUNICATION
4.0 CASE COMMUNICATION

All communications with laboratory staff that form the basis for decisions in casework must be
documented in the case. These communications may include, but are not limited to, communications
with the agency, the District Attorney, another FSD lab or staff member, or any combination thereof. The
date, with whom the communication took place, who initiated the communication, and the conversation
(in substance) will be documented.

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5.0 GENERAL EVIDENCE HANDLING

It is important that evidence be collected, handled, and stored in a way that will ensure its integrity.
5.1 General Evidence Handling Guidelines

 Protect yourself and others
 Protect the evidence
 Consider all types of forensic evidence
 Document the chain of custody
 Document the location of evidence recovery with notes, sketches, and/or photographs
 Mark the evidence and/or packaging with a case identifier, description of evidence, initials,
and date
 Package each item of evidence separately Allow wet biological stains to air dry
 Obtain standards, when needed, for comparison to evidence
 Use packaging that is appropriate for the specific type of evidence, such as paper bags,
envelopes, plastic bags (for non-biological evidence), cardboard boxes, metal cans, glass
jars, tamper-proof sealing, etc.
 Select a sufficiently large package to allow the item to be re-sealed after examination.
Conversely, avoid oversized packaging when submitting small quantities of evidence (e.g.
residues).
5.2 Evidence Collection
5.2.1 Packaging
The type you choose depends on the type of evidence, the condition of the evidence, and the
examination(s) you want the laboratory to perform. Use the information in Table 1 below (or consult the
section specific to the type of evidence you have) to select the proper way to package it. All packaging
types should be clean and unused (e.g. no grocery bags that have previously been used.).

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Evidence Packaging
Packaging Type Uses
Paper bags or envelopes Most biological material (marijuana, psilocybin mushrooms, blood or
semen stained items, condoms, etc.). If unable to air dry prior to
packaging, submit to the laboratory as soon as possible and notify
them that it is a wet sample.
Plastic bags or Ziplocs Dry, non-biological material such as powder drug samples
Metal cans Arson evidence
Plastic buckets Samples from clandestine laboratories that are individually packaged
in glass vials and set in an absorbent material (e.g. vermiculite, kitty
litter, etc.) in the plastic bucket.
Glass or plastic container Liquid drug samples, syringe contents, samples from clandestine
laboratory (glass containers only), miscellaneous trace samples, etc.
Paper folds and Post-It notes, Small pieces of trace evidence, hairs, fibers, minute glass particles,
then placed into a clean paint chips, residue amounts of powder drugs, etc. Place inside a
envelope larger paper envelope.
Use of Post-It notes: use either gloved fingers or a tool (e.g., forceps,
tweezers, etc.) to collect the trace evidence, place on the adhesive
of a post-it note, and then fold the note over on itself and label.
Cardboard boxes Firearms, knives, large pieces of plate glass, a piece of flooring with
a shoeprint, etc. Offers protection from sharp edges and the depth
protects one surface of the evidence from rubbing.
Sharps tube syringes
5.2.2 Sealing
A proper seal ensures that evidence has not been accessed, altered, compromised, or lost during
storage/transportation. Evidence seals must be tamper-evident (e.g., heat seals, tamper-evident
adhesive seals, tamper-evident tape, etc.). Sealed evidence should be initialed and, when possible, the
initials should cross over the seal in such a way as to provide visual indication of entry into the evidence
packaging if the seal is broken. Staples and other sealing techniques can be used in addition to the
acceptable tamper-evident seal.

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Evidence must be sealed before submission to the laboratory:
Figure 3: Acceptable Figure 3: Not Acceptable
5.3 Chain of Custody

The FSD maintains a chain of custody for all evidence from time of receipt at the lab to when it is
returned to the agency. Each laboratory utilizes a secure electronic chain of custody record through the
JusticeTrax Laboratory Information Management System (LIMS) for all evidence submitted to the
laboratory regardless of method of submission. For evidence that is personally delivered, the initial
COC (when evidence is received in the laboratory) is documented on the Form 49 with a date, time and
signature of person receiving the evidence submission. For evidence that is delivered via USPS, UPS or
the State of Oregon Shuttle, the initial chain of custody (when evidence is received in the laboratory) will
be documented by the person who signed for/received the shipment either on the Receipt of Shipment
Log or on the Form 49.
5.3.1 Submission of Evidence to Your Forensic Laboratory

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Evidence should be submitted to your local laboratory, even if your local laboratory does not offer the
service you are requesting, unless it falls under one of the exceptions listed below or there is verbal
approval to send the evidence directly to another lab. For example, although DNA analysis is only
performed at the forensic laboratory in Portland, you should still submit all evidence needing DNA
analysis, including standards, to your local laboratory.
Exceptions: DNA cases submitted under the High Throughput Property Crime program, arson, blood
alcohol, toxicology, and emailed images for footwear or tire make/model search.
Prior to submitting a large quantity of non-drug evidence in a single case, it is recommended that a
meeting be arranged with an analyst in your local laboratory to triage the evidence Refer to Chemistry
section in this manual for guidance on submission of a large quantity of drugs. OSP Forensic Scientists
are trained to manage the evidence in your case by ensuring that the evidence gets to the appropriate
section for examination. That section may be in another laboratory and the evidence will be transferred
to them without any additional request by you. The analyst that is managing your case evidence in your
local laboratory will be your contact person for status updates.
Sealed evidence may be submitted to the forensic laboratory by one of the following methods:
 Personal/Individual delivery (please call for an appointment before delivering to the lab)
 First Class U.S. Mail
 Federal Express
 United Parcel Service (UPS)
 Electronic transfer of digital images or data (Latent Print images are not accepted in this manner)
For all methods, care should be taken to ensure that evidence is not lost, damaged, or contaminated,
and that the chain of custody on all exhibits can be established and maintained.
The Department of Transportation has Federal Regulations on packing and shipping items.
Upon receipt, the evidence packaging will be checked for an acceptable seal. If evidence is received
improperly sealed, the submitting officer or a laboratory staff member must seal and initial the evidence.
Evidence items received will be inventoried against the Forensic Services Request Form (Form 49) to
ensure that the items indicated on the Form 49 are the items received. If a discrepancy is noted it will be
documented on the Form 49 and the customer notified.
5.4 Forensic Services Request Form
5.4.1 Laboratory Analysis Requests

A Forensic Services Request Form (Form 49) or equivalent documents the agency’s request for service
and should be submitted with the evidence. A single Forensic Services Request Form may document
multiple requests for service in any of the disciplines available through the FSD. Additional requests for
service may be generated by the FSD as a result of communication with the submitting agency or DA.

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The reason for creation of such a request and any associated communication must be documented in
the lab case record. The Form 49 is available at
http://www.oregon.gov/osp/FORENSICS/docs/ospform49sept201.pdf, or through the local Forensic
Laboratory or State Police Office. Much of the form is self-explanatory; however, a description of what
each field in the form means is described below.
 Rush—indicate due date and reason for rush (e.g. trial date, public safety concern)
 Additional Suspect Information Only and Previous Evidence Submitted to this/any
Laboratory – check if either applies.
 Agency – fill in your agency. List all involved agencies.
 Agency Case # – if more than one case number applies to the evidence being submitted, list all.
 Case Restriction—see back of Form 49 for more information.
 Offense – fill in all offenses associated with the case (be specific).
 Offense Date –date the crime occurred, not the date the evidence was collected or turned into an
agency’s property room.
 County of Venue – District Attorney’s Office that will have access to a copy of the laboratory
report. Fill in the county that the case will be prosecuted in, which may not necessarily be the
county your agency is in.
 Names of Involved Individuals –all victims, suspects, and other involved individuals. Complete
all fields if known.
 Investigating Officer and phone number – name of primary investigating officer and phone
number
 Submitting Officer –individual that delivered, mailed, or shipped the evidence to the laboratory.
 Agency Exhibit – fill in your exhibit numbers for the evidence that is being submitted.
 Description of Evidence – describe the evidence that is being submitted. The list of evidence
should be exactly the same as the evidence being submitted. Do not list additional evidence that
was not submitted, or submit evidence that is not listed. Indicate from where/whom each
evidence item was collected, if known.
 Exam Requested – this is what you want the laboratory to do with the evidence. Be as complete
as you can with your request or use the drop-down menu. If you do not know what request is
appropriate, please contact your local laboratory for assistance. The Forensic Services Division
Laboratory reserves the right to select appropriate methods of analysis based on the type of
evidence and information provided.
 LEAVE SHADED AREAS OF THE FORM BLANK.

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5.4.2 Evaluation of the Request for Analysis

Prior to initiating testing of evidence, FSD personnel will evaluate the request(s) for service, the
evidence items, and case history provided. The primary review of the request for service will consist of
a review of the service(s) requested by the customer in order to confirm that the FSD can provide the
services requested. If, upon primary review it is reasonably believed that the FSD can perform the
requested service, this review will be documented by entering the appropriate request(s) in the
JusticeTrax Laboratory Information Management System (LIMS). The OSP FSD will have initial
discretion over the selection of methods for analysis, the totality of the analysis, and the items tested.
5.4.3 Agency Reports

An agency report specific to the items of evidence submitted should accompany the evidentiary items
for all requests with the exception of toxicology, drug chemistry, blood alcohol, methamphetamine
quantitation, and anonymous analysis requests.
5.4.4 Rushes
If your request is a “Rush”, indicate so in the appropriate location on the Forensic Services Request
(Form 49), along with the date the request needs to be completed, if known. Common reasons for
“Rushes” are upcoming grand jury and trial dates, and an immediate need for investigative leads.

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6.0 LABORATORY REPORTS
6.0 LABORATORY REPORTS
6.1 Laboratory Online Information System (LOIS)

Electronic reports and case status is accessible through the Laboratory Online Information System
(LOIS). Anyone who is a member of an agency serviced by the Oregon State Police Forensic Services
Division, and needs access to the work done on behalf of their agency, may request LOIS access. The
help button at the following website contains the procedure for accessing LOIS,
https://xn.osp.state.or.us/LOIS/Login.aspx.
A final report is released electronically to the agency/customer that made the request for service through
the Laboratory On-line Information System (LOIS). An automatic notification of the report availability is
also sent to a designee at the District Attorney’s office that serves that agency/customer.
6.2 Forensic Laboratory Evaluation Form and Surveys

Periodically, the Forensic Services Division will send evaluation forms or surveys to its service agencies.
The following are the different categories of evaluation forms or surveys that may be sent:
 Intoxilyzer (sent via LOIS)
 Crime Scene Investigation
 Court Room Testimony
 Laboratory Analysis (sent via LOIS)

These evaluations and surveys provide valuable feedback on our goal of providing quality and timely
scientific, technical, and investigative support. Should you receive an evaluation form or survey, please
take the time to provide your feedback.

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7.0 FIELD INVESTIGATIONS (CRIME SCENES)

OSP FSD is available to assist with crime scene investigations at the request of any law enforcement
agency, District Attorney’s Office or the State Medical Examiner’s Office. A request can be made during
normal business hours (8:00am to 5:00pm, Monday -Friday) by calling your local laboratory (see
laboratory contact information).
After hours, call OSP Regional Dispatch Center at 541-776-6111 (Southern Regional Dispatch Center) or
503-375-3555 (Northern Regional Dispatch Center) and they will contact the on-call forensic laboratory
supervisor for you.
Laboratory management will assess each request based upon the crime type, the complexity of the
forensic services needed, available resources within the laboratory and the investigating agency, and the
anticipated response time. Crime scene response is primarily limited to homicides/attempted homicides,
skeletal remains and buried body recovery and officer-involved shootings. If special circumstances exist
which require the skills of trained forensic personnel, crime scene assistance may be provided for person
crime cases such as assault and robbery. The FSD does not respond to property crimes cases but FSD
resources are available to consult via telephone for any crime scene
If a search warrant is deemed necessary, it should be procured prior to the arrival of laboratory staff and
available for them to review. It is the responsibility of the investigating agency to provide crime scene
security for the duration of the crime scene processing.
7.1 Crime Scene Services Offered

Evidence identification, documentation, preservation, and collection
Buried body excavation and scattered remains recovery
Crime scene photography
Bullet trajectory determinations
Bloodstain pattern analysis and reconstruction
Shooting scene reconstruction
Locating occult blood
Alternate light source processing
Impression enhancement, casting or lifting (e.g., shoe or tire)
Metal detection
Latent print processing
Trace evidence collection
Body processing
Vehicle processing
Autopsy photography and assistance
Crime scene reconstruction

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7.2 Processing a Crime Scene

Crime scene documentation consists of notes, photographs and diagrams/sketches. Using a
combination of these techniques will create a detailed record and will provide a basis for the
investigator’s future recollection of the scene.
7.3 Notes
Crime scene notes should be clear, legible, detailed, and should be taken in chronological order
throughout the processing of the crime scene. Observations (e.g., description of the body, weapons, and
bloodstains), processing techniques and results (e.g., dusted with black fingerprint powder and lifted one
print) should be documented.
7.4 Photography
Photographing a crime scene should be performed in a systematic manner to ensure that all necessary
photos are taken. Photographs should to be taken before any evidence is moved. The best approach
to crime scene photography is to sequentially take overall, midrange and close-up photographs.
 Overall Photographs
These photographs show the relationship of evidence in an area (e.g., photographs of a living
room showing a body and numerous other items of evidence), or document the location of a
crime scene (e.g., a photograph of the outside of a house showing the street number).
Overlapping photos are recommended to ensure that the entire area is documented. If placards
are utilized to identify the location of evidence at the scene, photos should be taken before and
after their placement.
 Midrange Photographs
These photographs document the relationship of evidence items to other items within an area.
 Close-up Photographs
These pictures show the detail of an individual item of evidence. Close up photographs should be
done with and without a scale.
 Examination-quality Photographs
High-resolution, close-up photographs containing a scale, taken of specific items in a manner to
capture maximum detail. Examination-quality photographs are required for footwear and tire
impression and latent print comparisons.
7.5 Diagrams/Sketches
Scene diagrams/sketches can provide an overall view of a scene and the location of evidence items.
They can also be used to document detail of specific items or areas.
Accident Reconstructionists should be utilized to diagram the scene whenever possible. The diagram
should include all relevant measurements (e.g. dimensions of the room, measurements to all evidence

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from two independent points, heights of objects/evidence for a 3-D computerized rendition of the scene,
etc.)
A key/legend is necessary if you are using item numbers or evidence markers to show the locations of items
on the sketch.
7.6 Conduct a Detailed Search

Conduct the search of the crime scene using a systematic search approach. Room-to-room searches
and logical association searches (e.g., following a blood trail from one point to another) are commonly
used indoors. For outdoor scenes, areas are typically searched in overlapping sections.
7.7 Vehicle Examinations

Note the following information about the vehicle:
 Year
 Make
 Model
 License plate
 VIN
 Color
 Damage
Below is a list (not all-inclusive) of things to consider, based on the case circumstances, when
examining a vehicle. It may help to divide the interior into quadrants and search each quadrant from
ceiling to floor.
 Examine the exterior of the vehicle. Look for transfer evidence relevant to the case (e.g. paint
from a hit-and-run vehicle, clothing impressions on the plastic of a front bumper, hair caught on
the broken edge of windshield glass, etc.).
 Document tire information for all tires including manufacturer, type, size, and tire pressure. If the
tires need to be compared to tire track evidence the forensic laboratory should be contact to
assist in collecting to an exemplar from each tire.
 Note the position of seat(s), tilt wheel, accelerator and brake pedals, and rear view mirror.
 Vacuum or tape lifts of specific areas for trace evidence.1
 Examine the interior of the vehicle and the trunk for potential evidence.
 Examine the engine compartment and undercarriage of the vehicle for potential evidence.
 Take known standards from the upholstery, carpet, or other vehicle for possible later
comparisons.
 If paint or glass damage is apparent, take known standards from the broken glass and areas of
damaged paint.
1 See “Fiber Evidence – Collection of Fiber Evidence.”

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7.8 Body Processing

To prevent potential loss of fragile biological evidence, it may be necessary to collect swabs from the
body at the crime scene prior to transporting it to the medical examiner’s office for autopsy. Take into
consideration the position of the body, the context of the scene and the case scenario in deciding when
to collect. To achieve optimal collection of the evidence listed below, a total of two water-moistened
swabs should be used. Information about what evidence was collected at the scene should be
disseminated to the deputy medical examiner or agency representative so that additional collection of the
same swabs is not duplicated during autopsy.
Evidence of: Collect the Following:

Manual strangulation Swabs from neck and behind ears
Ligatures/bindings Swabs from ends of ropes/ties
Dragging Swabs from ankles/arms/wrists, as applicable
Pocket-rifling Swabs from turned-out pockets
Stretched/torn clothing Swabs from stretched/around torn areas
Possible sexual assault Swabs from inner thighs2
7.9 Postmortem Examinations

Photograph the following:
 Full body photographs of victim as delivered to the postmortem examination.
 Full body photographs of victim unclothed, before and after clean up.
 Mid-range and close-up photographs of exterior wounds and/or identifying marks (e.g. tattoos)
with and without a scale.
 Photograph any body parts or evidence requested by the Medical Examiner (e.g. bullets, bullet
tracks, etc.).
It is recommended that the following evidence be collected during a postmortem examination.

Remember that the postmortem examination may be the only (or last) chance to collect this evidence,
so it is prudent to collect more than you think you need, rather than less, in these situations.
 DNA standard. Either a blood standard on filter paper or swabs, one lavender/purple-stoppered
EDTA tube or oral swabs can be collected. For recently transfused decedents, an oral swab
standard is an appropriate DNA standard.
 Blood sample for blood alcohol and/or toxicology. Collect at least two (2) gray-stoppered tubes.
Femoral blood is preferred; however, blood drawn from other sources can also be analyzed.
 Urine sample for toxicology. Collect at least one (1) red-stoppered tube.
 Head hair standard from various areas on the head. Standards must be pulled, not cut.
 Pubic hair standard from various areas in the pubic region. Standards must be pulled, not cut.
2 A full SAFE kit is the responsibility of the Medical Examiner and can be collected at autopsy.

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 Sexual Assault Forensic Evidence (SAFE) kit3

 Penile Swabbing Forensic Evidence (PSFE) kit
 Fingernail scrapings
 Clothing, one item per bag
 Evidence items located in or on the body
 Finger and palm prints (for elimination purposes and identification)
7.10 Decomposed Remains

If the human remains are too badly decomposed, it may be impossible to obtain an adequate oral or
blood standard and thus necessary to collect an alternate type of DNA standard. Contact your local
laboratory for instructions on other collection and preservation options.
7.11 Bloodstain Pattern Analysis

Analysis of bloodstain patterns can reveal significant information in some cases. In such cases, the
relevant issue may be how the blood was deposited rather than from whom the blood came. When this is
the situation, contact your local laboratory for a qualified expert to assist in the bloodstain pattern
interpretation.
3 Consider collecting this evidence even when sexual assault is not immediately suspected. If it is not collected and becomes
relevant later in the investigation, the samples may be difficult or impossible to recover. See “Biological Evidence – Sexual
Assault Evidence Kits and Penile Swabbing Evidence Kits” for further information.

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8.0 MISSING PERSONS
8.0 MISSING PERSONS (‘MP’)

If a Missing Person case remains active and unresolved after 30 days have elapsed, a DNA sample
directly associated with the missing person should be obtained. DNA Analysis on the majority of missing
person cases should be performed by the University of North Texas Center for Human Identification
Laboratory (UNTCHI). The law enforcement agency that accepted the missing person report shall
attempt to obtain a DNA sample directly associated with the missing person (Direct Reference Sample,
described below) or from biological family members of the missing person (Family Reference Standard,
described below). Documentation necessary to enable the agency to use the sample in conducting
searches of DNA databases should be provided and can be found within the collection kits. Collection
kits can be obtained by contacting the University of North Texas at 1-800-763-3147. The local laboratory
can also assist with obtaining these collection kits.
8.1 Secondary Standards or Direct Reference Sample

Agencies should attempt to obtain DNA samples known to be from the missing person. Known sources
of DNA may include:
 Previously collected medical specimens (which may have been stored at a hospital or clinic).4
 Personal items, items used only by the missing person or rarely used by anyone else (e.g., a
toothbrush, lipstick, or other item containing saliva or blood). A family reference standard (see
below) must also be submitted to confirm the personal item is from the missing person.
Other Secondary Standards (not preferred)

 Hairs are not the preferred direct reference sample because of various concerns and
contamination. UNT discourages hair submission if at all possible and asks agencies to work to
get a better sample. When hairs are the only direct reference sample available UNT requires that
a qualified hair examiner examine the hair to determine if the hair was human and that roots were
intact. The report and the examiner’s credentials would need to be accompanying the submission
from the agency.
 Baby teeth are not preferred due to the potential for mix-ups with other children’s teeth and the
possible lack of mitochondrial DNA.
Additional Note:
 The University of North Texas will not accept clothing as a direct reference sample due to
contamination with other mitochondrial DNA. Both a nuclear DNA profile and a mitochondrial
DNA profile will be completed by UNT in order to obtain a match to unidentified remains.
4 Include information from the medical facility detailing how the sample was stored (i.e., was a fixative like
formaldehyde or formalin used). The use of fixatives may compromise the ability to obtain a usable DNA profile.

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8.0 MISSING PERSONS
These items should be submitted directly to the University of North Texas. Your local laboratory is
available for questions.
8.2 DNA from the Missing Person’s Relatives (Family Reference Standards)
When medical specimens and the personal items mentioned in the section above are not available, DNA
testing can be done on samples from biological (blood) relatives. DO NOT collect DNA samples from
adoptive parents, adopted children, stepparents and other non-biological relatives. DNA from these
relatives cannot be used to identify the missing person. The closer the family relationship to the missing
person the more likely a match can be made. It is more difficult to make a match between decedent
remains and distant relatives. In some cases, it is useful to have DNA samples from specific relatives. If
DNA from the missing person’s children is used, it is helpful to have DNA from the children’s other
biological parent (mother or father).
Preferred Family Reference Samples in order of preference
1. Both parents or known identical twin

2. One parent, spouse and children
3. Children and spouse
4. One parent and sibling
5. Siblings (two or more)
8.3 Family Reference Standard Collection Kit

This is the kit produced and disseminated by the University of North Texas to collect oral swab DNA from
family members of missing persons. These kits will be collected by the law enforcement agency involved
in the missing person investigation, and entail collecting oral cells on swabs, packing the swabs correctly
and fully completing the proper documentation provided. Collection kits are available with instructions
and supplies for retaining the DNA samples from family members (contact the University of North Texas
at 1-800-763-3147).
Each kit is for one family member. Several kits may need to be collected. The kits will be sent directly to
UNT for nuclear and mitochondrial DNA analysis. Do not submit these collection kits to the OSP
Forensic Laboratories. The University of North Texas has the capability to enter the completed DNA
profiles into the appropriate CODIS databases. For more information on the CODIS database go to the
following website: http://www.fbi.gov/about-us/lab/biometric-analysis/codis

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9.0 UNIDENTIFIED REMAINS

Refer to ORS Chapter 146 - Investigations of Deaths, Injuries and Missing Persons.
9.1 Unidentified Remains

“Unidentified human remains” do not include human remains that are part of an archaeological site or
suspect of being Native American. Archaeological remains are covered under ORS chapters 97 and 390
and ORS 358.905 to 358.961.
All unidentified human remains will be initially processed by the Medical Examiner’s Office. The OSP
Forensic Laboratories will not accept human remains with the exception of human hands for identification
purposes.
9.2 Identifying the Deceased

The State Medical Examiners (ME) Office is responsible for identifying deceased persons. The Forensic
Services Division may on occasion be requested to assist the Medical Examiner’s Office, or another
agency, with these identifications. Requests of this type should be made or coordinated through the
State ME’s Office. Friction ridge impressions obtained from unknown deceased persons may be
compared with known standards and/or searched through ABIS for the purpose of individualization or
exclusion.
Skeletal remains with all case documentation may be mailed to the Oregon State Medical Examiner’s
Office for anthropological examination. All agencies should contact the OSP-ME Anthropology
Section/Dr. Nici Vance at 971-673-8200 for instructions prior to sending the remains. All investigative
processes (e.g., dental record comparison, history/healed fracture comparisons, personal effects) will be
utilized in order to identify remains before DNA analysis will be attempted. If the remains are not
identified after these processes, they will be sent to UNT for nuclear and mitochondrial DNA analysis.
The decision for DNA analysis will be made by the Oregon State Medical Examiner’s Office.
Contact information:
Oregon State Medical Examiner’s Office
13309 SE 84th Avenue, Suite 100
Clackamas, Oregon 97015
971-673-8200
DNA Collection Kits for Family Reference Standards

University of North Texas
1-800-763-3147

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Additional online resources:

The Oregon State Police Medical Examiner Division has all of the necessary paperwork for law
enforcement to successfully submit Direct Reference Samples or Family Reference Standards to the
University of North Texas on their website: http://www.oregon.gov/OSP/SME/pages/index.aspx.
Access these forms under the heading, “DNA Submission Forms for Law Enforcement & Families”.
These forms can also be found on the OSP Forensic Services Division website, located under the
heading “Law Enforcement Agency Resources: National Missing Persons Programs”, found here:
http://www.oregon.gov/osp/FORENSICS/Pages/OSP%20FORENSICS.aspx
The National Missing and Unidentified Persons System (NamUs) is the first national online repository for
missing persons records and unidentified decedent cases. For more information on NamUS go to the
following website: http://www.namus.gov/.

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10.0 BIOLOGICAL EVIDENCE

Biological fluids such as blood, semen, and saliva are frequently encountered as physical evidence in
many types of criminal investigations such as homicides, sexual assaults, assaults, and robberies.
10.1 Safety and Contamination Prevention

Current DNA technology allows for very small amounts of sample to be analyzed. For example, a
bloodstain the size of a sharpened pencil point may be enough to perform DNA analysis on, as would the
residue amount of skin cells on the inside rim of a ball cap. Because of this, inadvertent contamination of
the evidence is possible if you do not take proper precautions. Individuals who routinely collect biological
evidence may want to work with their local laboratory to submit a DNA elimination standard for profiling,
so they may be ruled out as possibly having contaminated a particular item of evidence. Precautions to
guard against contamination include:
 Wear gloves and, as appropriate, a mask and eye protection while collecting biological samples.
 Change gloves frequently or anytime your gloves are contaminated with biological material.
 Avoid touching the tips of cotton swabs with your fingers or to other unintended surfaces
 To prevent contamination when collecting swabs, avoid talking over the swabs, blowing on the
swabs to make samples dry faster, etc. Consider purchasing individually wrapped sterile swabs
(e.g., Bode swabs).
 Do not touch the water dropper bottle tip to any surface or evidence.
 Clean tools (such as scissors or tweezers) that you might use to collect evidence with a dilute
bleach solution or product containing bleach. Alcohol (ethanol and methanol are both acceptable)
should be used to rinse residual bleach from those items that will come in direct contact with the
evidence. Do not use commercial disinfectant products (such as Clorox wipes) for the purposes
of decontamination unless they contain bleach or are designed specifically for laboratory surface
decontamination.
 Do not lick envelope seals.
10.2 General Collection Guidelines

In general, wet or moist biological evidence should be dried and packaged into clean and previously
unused paper containers (e.g., envelopes, bags, cardboard boxes). Do not wrap the evidence first in
plastic and then inside paper (or vice versa) as these conditions could cause the evidence to degrade.
Package each item separately and properly label and seal the container. All evidence containing known
biological fluids should be marked with appropriate biohazard labels.

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Biological evidence is best maintained dried at temperature controlled room temperature (preferably
individually packaged in paper containers). If evidence cannot be air dried, liquid evidence should be
refrigerated (never frozen), and wet evidence should be frozen.
Biological Material: Storage and Laboratory Submission
Swabs or
Swabs or items
items with with
biological biological
material material
(including that SAFE
Blood standards cannot be Kits &
(liqui ) dried PSFE Fece
d) Urine Bones Hair [DRIED] [WET] Kits s
Frozen Never Best Acceptable Best Accepta Best
ble
Refrigerated Less
Acceptabl Accepta
Best than 24
e ble
hours
Temperature Less
Controlled than Less than
Acceptable Best Best Best
24 24 hours
hours
Room
Temperature Accepta Acceptabl
Acceptable ble e
Table adapted from The Biological Evidence Preservation Handbook: Best Practices for Evidence
Handlers
10.3 Alternate Light Source Searches

Use of an ultraviolet (UV) light, Woods lamp, or other alternate light source (ALS) may assist in the
search for biological stains. Such devices can be helpful in a search, given that many biological stains
such as semen and saliva may fluoresce, or appear bright, when viewed with an ALS in a darkened
room.
However, there are three important points to remember when using an ALS to assist with searching for
biological stains:

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1. Many other substances may exhibit fluorescence. Examples include but are not limited to urine,
food, drink, and laundry detergent.
2. Not all semen or saliva stains will necessarily fluoresce with an ALS.
3. Blood will not fluoresce when viewed with an ALS; rather, it will appear dark.
10.4 Blood Evidence

Blood evidence is common in violent crimes and property crimes. Bloodstains may appear red, reddish-
brown, tan, gray, or yellowish. Bloodstains may be undetectable to the unaided depending upon
evidence and stain characteristics. In addition to DNA, blood contains cells and proteins that allow the
laboratory to perform the following examinations:
 Presumptive blood testing

 Testing to indicate human or non-human origin
10.4.1 Collecting Dried Blood

If it is reasonable to transport the stained object, the entire item may be collected and submitted to the
laboratory. Be careful to seal all openings of a package since dried blood may flake off of an object. If the
stained object is not being transported, collect the blood by one of the following methods:
10.4.2 Swabbing
1. Moisten a sterile cotton swab using 1-2 drops of distilled water or tap water.
2. Gently swab the stain with the moistened swab tip until the swab thoroughly absorbs the blood.
Continue collecting the stain until it is either completely collected or a sufficient number of swabs
(up to 4) have been saturated. Attempt to concentrate the stain on as few swabs as possible.
3. Allow the swabs to thoroughly air dry.
4. Place the dried swabs in a paper container (e.g., paper envelope or bag). Swabs collected from
the same stain should be packaged together and facing the same direction.
5. Properly label and seal the container.
6. When the amount of blood is small, it is advised to select an unstained area adjacent to the
suspected bloodstain to collect (known as a substrate control). Repeat steps 1 through 5 on the
unstained area. Package the substrate control separately.
10.4.3 Cutting
This collection method may be desirable when the dried bloodstain is on an object such as the
upholstery of a car seat or carpeting. Use a clean, sharp knife or scissors to cut the stained area, leaving

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unstained margins surrounding the stain. Package into a paper container and properly label and seal the
container.
It is recommended that you consult with the District Attorney’s Office before destroying property since the
item may need to be repaired or replaced.
10.4.4 Collecting Liquid or Moist Blood – Large Quantity

1. Saturate 4-6 sterile cotton swabs with the blood.
2. Blood will coagulate so it is important to collect a good mix of clotted cells and serum.
10.4.5 Collecting Liquid or Moist Blood – Small Quantity

1. Attempt to concentrate staining on as few swabs as possible. Use swabs to collect the blood,
concentrating the blood onto each swab.
10.5 Saliva Evidence

Saliva stains are not usually evident from a visual examination. However, certain types of evidence
frequently contain traces of saliva (e.g., cigarette butts, drinking containers, adhesive surfaces of
envelopes, chewing gum, bite marks, masks, etc.).
10.5.1 Collecting Saliva Evidence

If the stained object is transportable, the item may be collected and submitted to the laboratory. If it is not
transportable, such as bite marks on a body, collect the saliva stain in the following manner:
1. Moisten a sterile cotton swab with 1-2 drops of distilled or tap water.
2. Gently swab the suspected saliva stain.
3. Follow with a dry swab to collect any remaining moisture.
4. Label the swabs as “wet” and “dry”.
the same area should be packaged together and facing the same direction.

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10.6 Semen Evidence

When the perpetrator of a sexual offense is a male, semen stains may be found on the victim as well as
on clothing, bedding, rags, upholstery and other objects. Semen stains may appear off-white, yellow, tan
or colorless and may have a crusted appearance. Semen stains may be undetectable to the unaided eye
depending upon evidence and stain characteristics.
Consider collecting the suspect’s underwear, pants, or other clothing items, as victim DNA could also be
transferred to the suspect or the suspect’s clothing.
10.6.1 Collecting Semen Evidence

Collect all suspected stained material (e.g., bedding, underwear or other clothing, etc.). Each item of
evidence should be packaged separately and carefully to prevent loss of any trace evidence (e.g.,
hairs) that may be present.
Evidence with damp stains should be air dried. Consider marking the location of a damp stain by circling
it with permanent marker, as it may not be visible when dry.
Clean paper should be spread under the item to catch any debris, which may be dislodged during the
drying process. Clean paper should be placed between items hanging next to each other to prevent
cross-contamination. Package each item separately in paper bags or envelopes, along with any paper
used.
If the semen stain is on an object that cannot be easily submitted to the laboratory, contact your local
laboratory for collection instructions.
10.7 Adult and Child Sexual Assault Forensic Evidence (SAFE-A and SAFE-C) Kits
Biological evidence associated with the body of a potential sexual assault victim needs special attention.
The victim needs to be transported to a medical facility for a sexual assault examination. This should be
done as soon as possible in order to preserve what remains of the biological evidence and to document
any physical trauma. Consider having photographs taken of any physical trauma and collecting blood
and/or urine as well. Attending medical personnel should collect the evidence by using the appropriate
Sexual Assault Forensic Evidence Kit provided by the Forensic Services Division.
In general, if more than 84 hours has elapsed from the time of the sexual assault to the time of the
medical examination, the chances of finding semen evidence in the body of a living victim are greatly
diminished, although it may still be possible to detect male DNA. However, it may be prudent to collect a
SAFE Kit if you have any doubts or concerns. This time range does not apply to deceased victims; it is
recommended that you collect a SAFE Kit from deceased victims regardless of the elapsed time.
Bathing, showering, and douching by the victim does not necessarily eliminate the possibility of finding
semen evidence on the interior of the body. A SAFE Kit should be collected under these circumstances.

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Showering or bathing may eliminate saliva, semen, and trace evidence deposited on the exterior of the
body.
Undergarments, worn by the victim during and/or immediately after the assault, are also good sources for
collecting semen and hair evidence. Package each clothing item separately.
10.8 Penile Swabbing Forensic Evidence (PSFE) Kits

If a male suspect is taken into custody within 24 hours of the incident and he has not showered or
bathed, a Penile Swabbing Forensic Evidence (PSFE) Kit provided by the Forensic Services Division
should be collected. The purpose of the PSFE Kit collection is to collect cells that may have been
transferred to the subject as the result of sexual contact. If a PSFE Kit is not available, sterile cotton
swabs may be used. Follow the below instructions or contact your local laboratory for instructions.
The suspect’s underwear should also be collected as such evidence may provide an excellent source of
the victim’s DNA.
Step 1: Collect two penis shaft swabs.

 Lightly moisten one sterile cotton swab with water. Swab the entire penis shaft, rolling
the swab as you go and being careful to swab in the creases and under the foreskin.
 Allow the swab to dry, and then place in an envelope; labeled “Penis Shaft Swab #1”.
 Repeat the swabbing with another moistened sterile swab.
 Allow the swab to dry, and then place in an envelope; labeled “Penis Shaft Swab #2”.
Note: Avoid swabbing the penile orifice.
Step 2: Collect two scrotum swabs

 Lightly moisten one sterile cotton swab with water. Swab the upper and front scrotum
area, rolling the swab as you go.
 Allow the swab to dry, and then place in an envelope; labeled “Scrotum Swab #1”.
 Allow the swab to dry, and then place in an envelope; labeled “Scrotum Swab #2”.
Step 3: Collect any dried/moist secretions on pubic hair

 Examine the pubic hair for dried or moist secretions.
 If observed, collect dried/moist secretions on the pubic hair by cutting the matted hair
and placing in an envelope labeled “Dried/Moist Secretions on Pubic Hair”.
Step 4: Collect two pubic hair swabs

 Lightly moisten one sterile cotton swab with water. Swab the pubic hair area at the
base of the penis.
 Allow the swab to dry, and then place in an envelope labeled “Pubic Hair Swab #1”.

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 Allow the swab to dry, and then place in an envelope labeled “Pubic Hair Swab #2”.
Step 5: Head hair standards

 Collect 24 pulled and shed hairs from various areas on the head.
 Place the hairs in the provided envelope.
Step 6: Pubic hair standards

 Collect 24 pulled and shed hairs from various areas of the pubic region.
 Place the hairs in the provided envelope.
 If pubic hair standards are not collected, please document why.
Step 7: Collect four oral swabs

 Swab the inside of mouth with four sterile cotton swabs.
 Allow the swabs to dry, and then place in an envelope labeled “Oral Swabs”.
 Mark the envelope with the purpose of collection (DNA standard or oral sodomy).
Step 8: Collect any additional evidence

 Rectum, bite mark, hickey, finger swabs, external body swabs, or other sites of
evidence transfer.
 Evidence can be collected using sterile cotton swabs and envelopes.
 Use four swabs to collect evidence from internal body sites (e.g., rectum).
 Use two swabs to collect evidence from external body sites (e.g., hickey).
 Place the swabs from each location in a separate envelope and write on the envelope
the body location of collection and the purpose of collection (For saliva? For semen?).
Step 9: Distribute forms and package evidence

 Place the evidence collected in an envelope and seal.
Step 10: Collect the subject’s underwear

 If the subject was not wearing underwear at the time of sexual contact or afterward,
collect the subject’s pants.
 Package clothing in separate paper bags and seal.
 Label each paper bag with the subject’s name.

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11.0 DNA ANALYSIS
11.0 DNA ANALYSIS

Since blood, semen, and saliva originate as liquids, they can quickly coat or penetrate surfaces on which
they are deposited and may be difficult to remove once dried. Because no two humans are genetically
the same (except for identical twins), these body fluids are unique to the person from which they
originate. Performing DNA analysis on these fluids or stains can result in a genetic profile which can then
be compared to DNA profiles obtained from reference standards or from other items of evidence.
The nature of DNA analysis lends itself to a computerized identification system; thus, DNA profiles from
qualifying items of evidence can be compared to the Combined DNA Index System (CODIS), a database
that maintains qualifying DNA profiles from convicted offenders, items of evidence, unidentified human
remains, and missing persons. In Oregon, this information is maintained at the state level. Searches of
the database also occur at a national level.
The type of DNA analysis currently performed at the Oregon State Police Forensic Laboratories can yield
much information.
DNA analysis can: DNA analysis cannot:
Provide an association between an Determine the age or race of the person

item of evidence and an individual (or who deposited DNA on an item
individuals) and give an estimate of the
rarity of the evidentiary profile in
support of the association.
11.1
Exclude a person from being the donor Determine how old a sample is
of an evidentiary DNA profile
Determine the gender of an individual Determine how a sample was deposited
who deposited DNA on an item (see “Crime Scene Investigations -
Bloodstain Pattern Analysis”)
Determine that biological material is Differentiate between consensual and

from a human non-consensual sexual contact
Body Fluid Analysis

DNA analysis can be highly successful when the sample in question originates from a body fluid (blood,
semen, and/or saliva), although other types of evidence (described below) are also appropriate for DNA
testing. Items suspected to contain body fluids should be submitted to the laboratory for examination.

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11.0 DNA ANALYSIS
11.2 Wearer DNA

Wearer DNA evidence results from repeated contact from (mostly) the same individual’s skin, and
includes items such as clothing items, baseball caps, and gloves. Items considered for wearer DNA
analysis should be submitted to the laboratory for examination. Note that the definition of wearer DNA
evidence can also be extended to items routinely handled by an individual but not necessarily worn, such
as backpacks and duffel bags, or tools brought to and left at a scene by a suspect.
11.3 Aggressive Handling DNA

Aggressive handling DNA evidence results from contact that is limited but not casual, which includes
items such as ligatures or victim’s clothing where the suspect groped or grabbed violently, or contact that
is casual but not limited, which includes items such as the steering wheel in a stolen vehicle. Items
considered for aggressive handling should be submitted to the laboratory for examination.
11.4 Touch DNA

Touch evidence is the category of DNA evidence originating from an item that has had both limited and
casual contact with an individual’s skin. This category primarily includes objects touched by an
individual’s hand for a short period of time, such as keys, door handles, gun triggers, light switches,
drawer handles, countertops, etc. If there is some ambiguity with respect to whether the item falls under
the touch or aggressive handling category, the DNA Unit should be contacted for more guidance.
Touch evidence will only be analyzed with prior approval from the DNA Unit Supervisor. The
case should meet all of the following criteria:
1. The case is a rape, attempted rape, homicide, or attempted homicide.
2. All other forensic avenues to identify a suspect or link a known suspect to the crime have been
exhausted.
3. A DNA result, possibly in conjunction with the CODIS database, is necessary to identify a suspect
or link a known suspect to the crime. Evidence items will not be processed to determine,
establish, or corroborate path of travel or location.
4. There should be a reasonable expectation that the suspect handled the item.
5. Every reasonable effort has been made to collect elimination standards from individuals who
have routine or recent contact with these items.
If a case does not meet the above criteria, a private DNA laboratory may be of assistance if a suspect
standard is also available. If there is no suspect, analysis by a private laboratory may be of limited value
unless the OSP DNA laboratory is involved in the submission process. Unless arrangements are made
between the private laboratory and the OSP DNA unit in advance of the private laboratory’s analysis of

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the evidence, the OSP DNA Unit is unable to accept profiles generated by private laboratories for entry
into CODIS.
11.4.1 Collecting Aggressive Handling or Touch DNA Evidence

Whether touch evidence or aggressive handling evidence, an abundance of caution should be used
when swabbing or packaging an item for submission. Touch and aggressive handling evidence should
be collected prior to the use of any latent print processing techniques on the item.
1. Moisten a sterile cotton swab with 1-2 drops of distilled or tap water.
2. Gently swab the area suspected of having been touched or handled.
3. Follow with a dry swab to collect any remaining moisture.
4. Label the swabs as “wet” and “dry.”
5. Allow the swab to thoroughly air dry.
the same area should be packaged together and facing the same direction.
11.5 Criminal Paternity

Because half of an individual’s genetic material is inherited from each parent, given the DNA profiles of a
child and a known biological parent, the likelihood of an individual being the second parent can be
established. DNA standards from the known parent, child, and alleged parent are needed for analysis,
although, in rare instances, testing may proceed without a standard from the known parent.
Criminal paternity cases for which there is no live birth (e.g. analysis of products of conception and/or
amniotic fluid collected during pregnancy) are accepted on a case-by-case basis. Please contact your
local laboratory before submitting evidence of this type.
11.6 Y-STR Analysis

Y-STR testing, which involves the analysis of genetic material on the Y-chromosome (male
chromosome), is useful in DNA mixtures where there is an abundance of female DNA relative to the
amount of male DNA in a sample. Suspect standards are needed for comparison, as the overwhelming
majority of convicted offender samples contained in the state CODIS database have not been profiled
with Y-STRs. Because the Y-chromosome is passed from father to son, men in the same paternal
lineage (fathers, sons, brothers, uncles, etc., all having a common male ancestor) will generally have
exactly the same Y-STR profile. Traditional nuclear DNA analysis will be prioritized over Y-STR analysis.
11.7 DNA Standards

DNA standards should be obtained from all listed individuals in a case who may have contributed DNA to
evidentiary items if those items will be referred for DNA analysis. This includes victim and suspect
standards, and, in sexual assault cases, standards from any recent (within 3 days) consensual sexual
partner(s). Elimination standards are especially critical when an item of evidence is collected from person
who can reasonably be assumed to have left their own DNA behind on an item, such as standards from
the wearer of an item of clothing (when a profile foreign to the wearer is the goal of analysis) or
standards from the regular driver(s) of a vehicle when submitting swabs from the steering wheel of a
stolen vehicle. These standards are compared to the DNA profiles obtained from the evidence to

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11.0 DNA ANALYSIS
determine inclusions and exclusions. DNA profiles from convicted offenders will not be used in lieu of
DNA standards for direct comparison to evidence profiles.
For living individuals, oral swabs (also referred to as buccal swabs) are recommended for routine use as
a DNA standard; however, blood samples are also acceptable. Blood standards are preferred as a DNA
standard for deceased individuals that are not severely decomposed or have not recently received a
blood transfusion. For recently transfused decedents, an oral swab standard is an appropriate DNA
standard.
It is not recommended to swab the bleeding wounds of a living individual for submission as a DNA
standard in lieu of collecting oral swabs. Depending on the activity that led to the wounds, this type of
sample could yield a mixture of DNA from more than one individual, no longer qualifying the sample for
use as a DNA standard.
11.7.1 Collecting Oral Swab Standards

1. Vigorously swab the inside of the mouth until 2-4 swabs are collected. The individual may do this
him/herself under your direct supervision.
2. Allow the swabs to air dry thoroughly.
3. Seal in a paper envelope labeled with the individual’s name, date, and “Oral Swab Standard.”
The swabs collected from one individual should be packaged together.
11.7.2 Collecting Blood Standards

1. Collect at least one vial of blood into a lavender top tube, which contains the preservative EDTA.
If the blood is being collected from a decedent,
2. 4-6 swabs of blood may be collected instead of a vial of blood.
3. Label the vial with the individual’s name and date collected, and then seal it in a box or padded
envelope to prevent breakage. Label the outer packaging with the individual’s name, date, and
“Blood Standard.”
4. Never store liquid blood tubes in the freezer. Refrigerate them instead.
11.8 Decomposed Remains

If the human remains are badly decomposed, it may be impossible to obtain an adequate oral or blood
standard and thus be necessary to collect an alternate type of DNA standard. Contact your local
laboratory for instructions on other collection and preservation options.

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11.0 DNA ANALYSIS
11.9 Secondary Standards

When a person of interest is unable to be located (e.g., missing person, fled area, etc.) or a standard can
otherwise not be obtained (e.g. lack of probable cause for a warrant), secondary standards may be used.
Suitable secondary standards include cigarette butts, drinking containers, or personal care items (e.g.,
toothbrush, razor, etc.) known to have been used only by the person in question. Note that if a mixture of
DNA from more than one individual is returned from a secondary standard, it is no longer suitable for use
as a secondary standard.
11.10 No Available Suspect Standards

Evidentiary items may still be referred for DNA analysis even if no suspect standards are available, as
the DNA profiles from the evidence may be suitable for comparison to the CODIS database. It is
important to note that in the event of a probative association to a CODIS profile, a known standard (e.g.,
oral swabs) will likely be required for confirmation.
11.11 High Throughput Property Crimes Program

The Oregon State Police Forensic Services Division has partnered with several agencies across the
state to perform high throughput analysis of property crime samples. After receiving training from
Forensic Services Division experts, samples are collected by law enforcement personnel using specially
designed collection kits. Only Bode swabs and cigarette butts are permitted under this program, and a
maximum of three evidentiary items may be submitted per request. Once collected, the evidence is
submitted directly to the Portland-Metro laboratory using a Form 49 specifically designed for this
program. The goal of the DNA unit is to return the results of the analysis of these cases within 30 days
of submission to the laboratory, resources permitting. Under this program, all necessary elimination
standards must be submitted prior to the initiation of DNA analysis, and the participating agencies agree
to hold all non-DNA requests (e.g. latent print or footwear analysis) pending the DNA results. Please
contact the DNA Unit Supervisor at the Portland-Metro Laboratory (971-673-8230) for more information
about this program.
11.12 Private Laboratory Analysis

The Oregon State Police Forensic Services Division is unable to offer DNA analysis for urine,
mitochondrial DNA, drug paraphernalia associated with possession of a controlled substance (PCS), and
cases in which there is no victim.
For information regarding private laboratories available to conduct DNA testing, please contact your local
forensic laboratory. Please note that if evidence analysis by a private laboratory is performed without
prior consultation with the Oregon State Police Forensic Services Division, it may be impossible to
search any profiles resulting from the private laboratory’s analysis within the CODIS database.

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12.0 LATENT PRINT EVIDENCE

Friction ridge detail, also referred to as latent prints, is a widely recognized means of identification. Most
crime scene evidence has the potential to reveal comparable latent print impressions; therefore, one
should handle all evidentiary items with caution (i.e. with gloves and minimal contact). The chemical
composition of latent print residue is such that chemical techniques can be used to visualize latent prints
on both porous and non-porous substrates.
The services the Laboratory provides include the following:

 Examination and processing of evidence for latent
prints.
 Determination as to whether latent prints are of
comparison quality.
 Comparison of these latent prints in an attempt to
identify a source.
 Search for a possible source by utilizing the
Automated Biometric Identification System (ABIS)
and/or the FBI’s Next Generation Identification
(NGI) database.
12.1 Collection, Packaging, and Submission of

Evidence
In most circumstances, smaller items of evidence should be collected and submitted to the Lab for full
sequential processing. In this case, no processing (photographs with scales, dusting, etc.) should be
conducted by the agency unless absolutely necessary. Latent print evidence should be packaged in a
way to minimize the movement of the object without being too restrictive to cause wiping/rubbing (this
may destroy latent prints present on the object). Latent print evidence should never be in direct contact
with “packing peanuts.”
If items have been processed prior to submission to the laboratory, information regarding what type of
processing was performed should be documented and submitted with the evidence. Chemical
processing of evidentiary items should only be done in a laboratory setting.
Evidence exposure to water or dampness should be avoided. However, this exposure does not
necessarily destroy all latent prints. It is important that any wet or damp object be air-dried before it is
packed for shipment.
Paper items may be placed in a single envelope package for submission

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Guide to Collection and Packaging of Latent Print Evidence

ACCEPTABLE NOT ACCEPTABLE
DO use gloves to pick up items of evidence being DON’T apply powder to obviously greasy, wet or
careful not to wipe possible latent prints off the bloody surfaces, or to prints left in dust or soft
surface. putty. Please photograph these latent prints or
submit the items to the laboratory for processing.
DO minimize handling of evidence. DON’T wrap nonporous items in cotton or cloth

as they may damage or destroy latent prints.
DO fasten down large articles to a rigid surface to DON’T use “packing peanuts” directly in contact
prevent shifting and contact with other items. with the evidence.
DO put latent lift cards in envelopes and mark DON’T directly cover evidence to be examined for
and seal. latent prints with evidence tape.
DO take complete and legible inked fingerprint

and palm print standards of all subjects without
SID numbers and/or FBI UCN numbers who may
have handled the evidence.
DO include full name, date of birth, and SID

numbers for all involved persons on the Form 49.
DO place evidentiary papers and documents in

manila envelopes, seal and submit to the
laboratory
12.1.2 Digital Imaging

Visible latent prints can also be preserved via digital imaging. All digital images must be captured in a
lossless format (e.g., TIFF or RAW), contain a visible measuring device and have a resolution of at least
1000 pixels per inch (ppi) whenever possible. An overall photograph (in addition to the close-up image of
the impression) should be taken of the item (or scene) such that the latent print can be re-located on the
item (or within the scene). Images should be physically submitted to the laboratory via disk or thumb
drive.
12.1.1 Latent Print Lifts

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Larger objects that cannot be easily transported or packaged may be processed in the field. Processing
may be performed by powdering the surface with an appropriately colored fingerprint powder and lifting
any visible latent prints. If possible, an image of the latent should be acquired prior to lifting. Multiple
prints in close proximity should be collected on the same lift, if possible. In some instances, one may
need to perform multiple lifts of the same print to obtain the best quality lift. The location, orientation and
any other pertinent information (e.g. multiple lifts of the same impression) should be recorded clearly on
the back of the lift card. If it is unlikely that the latent print can be lifted, then attempt to collect and
package the evidence for submission to the Laboratory
12.2 Submission of Inked Prints for Comparison Purposes

The investigator should take inked prints from all persons known to have handled the evidence
(elimination prints) to permit comparison with any latent prints located on the submitted evidence. If the
individual already has prints on file, their full name, date of birth, and SID number or FBI/UCN
number must be listed on the Form 49.
Often latent prints found at the scene of a crime involve areas of the palms, second and third joints of the
fingers, and the finger sides and tips. For this reason, the analyst may request that the investigator take
clear and complete inked standards (major case prints) of all ridges on the hands of suspects or persons
known to have legitimately handled the evidence to aid in comparisons. Palm prints should always
include recordings of the lower finger joints, as well as the outer edge of the palm (writer’s palm). Inked
standards should be submitted with the evidence.
12.3 Automated Biometric Identification System (ABIS)

After comparisons are conducted with any known suspect’s prints, victim prints or submitted inked
standards, the remaining unidentified latent prints may be evaluated for ABIS quality and may be
searched against the Western Identification Network (WIN) ABIS and/or the FBI’s Next Generation
Identification (NGI) database. Latent prints that remain unidentified after a search in ABIS may be
registered in the Unidentified Latent Database (ULD) for future searches against new standards as they
are added to the database. The agency will be notified via a report if a registered print in the ULD is later
individualized. In the event the submitting agency should identify any latent prints the agency should
notify the laboratory so these latent prints can be purged from the ULD.
12.4 Identifying the Deceased

Refer to “Unidentified Remains” section in this manual.
Routine identifications for the purpose of clearing a Computerized Criminal History (CCH) record are
made through the Identification Services Section in Salem.
The following guidelines apply only when the item itself is not being submitted to the laboratory.

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13.0 DRUG CHEMISTRY
13.0 DRUG CHEMISTRY

Drug chemistry analysis is the identification of specific chemicals that are designated as controlled by the
Oregon Administrative Rules.
13.1 Analysis of controlled substances

There are three categories of analysis related to controlled substances:
 Controlled substance analysis – Qualitative analysis and identification of controlled
substances. In general, non-controlled substances are not identified (though exceptions do exist)
and Oregon law requires only the presence of a controlled substance be determined, not its
purity. Evidence can be found in both in liquid and solid samples (e.g. tablets, powders, plant
material, etc.), varying in size from a residue to multi-kilogram submissions.
 Clandestine laboratory analysis - Samples (controlled and non-controlled) are analyzed to
determine the method being used to manufacture an illegal drug. Theoretical drug yields based
upon the quantity of chemicals and precursors seized may also be possible. Typical clan lab
analyses involve methamphetamine and MDMA production – please contact your laboratory if
you suspect a substance other than one of these.
 Methamphetamine Quantitation – Quantitative (purity) testing is performed for federal
sentencing in methamphetamine cases only. Request by a US Attorney is required prior to
laboratory analysis.
 Add something about inhalants since they are now directed to Springfield Chemistry?
13.2 Submission Policies

Each laboratory normally limits analysis to two items per defendant, so the investigating officer should
use discretion and submit only those two items that are essential to the case. Exceptions can be made
by District Attorney’s Office request to the laboratory. In cases with multiple suspects, an indication
should be made as to what evidence belongs to which suspect. Further, if an item is specifically related
to a probable cause issue, it should be designated as such.
13.2.1 Large Quantities of Controlled Substances (not plant material)

When large quantities of controlled substances which are not plant material are submitted (e.g., more
than two kilograms of heroin, cocaine or methamphetamine), the laboratory will make an effort to
complete these cases in a timely manner. The agency will be contacted to pick up this evidence after
testing.
13.2.2 Large amounts of plant material (marijuana)

The material should not be submitted directly to the laboratory. A small sample of the material should be
removed, packaged, and submitted for analysis. In certain circumstances where a seizure’s entire
weight is needed, laboratory personnel can assist with the weighing of large submissions. It is

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13.0 DRUG CHEMISTRY
recommended that the submitting officer contact the laboratory prior to submission to discuss analytical
needs.
13.2.3 Pills, Capsules and Tablets

No more than two pills, capsules and/or tablets of each type should be submitted to the laboratory for
confirmation of the contents. An exception will be made for 3,4-methylenedioxymethamphetamine
(MDMA/ ecstasy), 3,4-methylenedioxyamphetamine (MDA), and 3,4-methylenedioxy-N-
ethylamphetamine (MDEA) pills, capsules and/or tablets if a laboratory report listing the total number is
needed for enhancement purposes. The number and description (e.g., color, shape, imprint) of pills,
capsules and/or tablets should be listed on the Laboratory Request Form (Form 49) and they should be
sealed in a clear plastic bag so that they can be seen when submitted to the laboratory.
13.2.4 Syringes
The following conditions must be met prior to submission of a syringe with a needle.
A.) The syringe must be the only drug item in the case or must be needed to establish probable
cause.
NOTE: If there are other suspected controlled substances submitted, we analyze those exhibits
first. In the event the other items do not contain controlled substances, then the syringe can be
submitted for analysis if still required for the case. The syringe will be analyzed when necessary
to support the probable cause even if other suspected controlled substances are present in the
case.
B.) The syringe must be properly packaged in a puncture proof container. If the evidence is
submitted in improper packaging, the syringe will be returned to the agency for proper packaging.
C.) The DA must agree to prosecute the case.
D.) Submission of syringes requires prior laboratory approval. The labs will grant approval if
conditions A through C above are met.
If the above conditions are not met and the officer still wants the syringe analyzed, then either the
evidentiary material must be removed from the syringe (i.e. empty the contents of the syringe into a
sealed vial) or evidence rendered safe (i.e. safely remove the needle from the syringe) prior to
submission to the laboratory.
13.2.5 Razors and Other Sharp Objects

Razors and other sharp objects should be separated from other evidence that requires analysis and
must be adequately labeled and packaged in such a manner as to protect personnel during handling.
The packaging must be protective enough to ensure no possibility of being injured or punctured.

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13.0 DRUG CHEMISTRY
Commercially manufactured puncture-resistant containers are available for purchase. Contact your
local laboratory for information.
13.2.6 Biohazards
Items removed from a body orifice as well as syringe contents should be labeled with a “BIOHAZARD”
label and the Form 49 should indicate from where the item originated as well as any identification of
known concerns (e.g., “suspect positive for hepatitis C”). Please do not label typical drug submissions as
“biohazard” unless there truly is cause for concern.
13.3 Collection and Packaging

All submitted items should be written on the Form 49. It is recommended to separate out drug evidence
from items not needing analysis (e.g. submit only the bindles of drugs, not the entire purse and contents).
Care should be exercised when packaging more than one exhibit in the same package to prevent cross
contamination. It is also recommended that exhibits which are loose, open, leaking, or have cut
corners/unsealed edges be individually secured to prevent loss. If such internal agency bags are used,
please label them as such to distinguish from the actual evidence.
13.4 LSD
Suspected LSD should be kept away from direct light. This can be accomplished by storing in dark
packaging or wrapping in foil. Additionally, LSD has a very low dosage activity, so extra care should be
taken to avoid exposure.
13.5 Plants/Wet Evidence

Live plant material and other damp/wet evidence should be dried prior to
submission. This includes marijuana, mushrooms, peyote, or opium poppies.
Fresh plant material, when packaged in airtight containers such as plastic
bags, decays rapidly and can inhibit or possibly eliminate the chance for
detecting a controlled substance. Suspected fresh khat should be frozen
and submitted as soon as possible. Potentially spiked liquids should be
submitted in a timely manner.
13.6 Latent Prints

Drug packaging that needs processing for latent fingerprints should be
separated from the drugs when possible. The evidence for latent fingerprint processing is then submitted
separately from the drugs.
13.7 Field Test Kits

When using commercially purchased field test kits, remember that a positive result with the kit only
indicates the possible presence of a drug. There can be other substances that give a similar reaction
(e.g. marijuana resin can turn brown in a test that normally turns orange in the presence of
amphetamines). Generally, the colors of a positive reaction indicated on the test kit are very apparent
and interpretation of the result is usually not necessary.

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13.0 DRUG CHEMISTRY
The field test kits should be discarded after use. Please do NOT submit them to the laboratory. Many of
the kits contain concentrated acids that can leak, creating a chemical hazard and potentially destroying
evidence or packaging. In addition, drugs tested in these kits cannot be further analyzed.
Officers should not use field test kits on a residue amount of drug. In cases where quantities are limited,
omit the field test and submit the evidence directly to the laboratory for analysis.
13.8 Clandestine Drug Laboratories

Clandestine drug laboratories range from crude makeshift operations to sophisticated and technologically
complex facilities. They can be set up anywhere and are often found in private residences, hotel and
motel rooms, trailers, barns and outbuildings, and commercial buildings. At these locations sophisticated
surveillance equipment and booby-traps may be set up to deter intruders and law enforcement personnel
from entering.
There are usually two situations when a clandestine laboratory is encountered. The first is when police or
fire agency personnel encounter a previously unknown lab. When this occurs the personnel should
secure the scene, allow no unauthorized or unnecessary entry, and contact the local specialized unit or
trained personnel to process clandestine laboratories.
When clandestine drug laboratory chemicals are detected during a vehicle stop, treat the site as a crime
scene. Contact the local law enforcement unit trained to handle this situation for assistance.
The second situation would involve prior knowledge by the local law enforcement agency of the illegal
activity. A raid is planned and personnel needed for the proper shut down and dismantling of the
laboratory have been contacted.
13.8.1 Types of Clandestine Drug Labs

Although methamphetamine is by far the most commonly encountered clandestinely manufactured drug
in Oregon, the OSP clan lab analysts are prepared to analyze samples from other types of manufacture.
These include but are not limited to amphetamine, methcathinone, MDMA & related compounds, PCP,
LSD and other compounds in the phenethylamine, tryptamine and piperidine classes.
13.8.2 Collection of Clandestine Drug Lab Samples

Extreme care and caution should be exercised whenever investigating or processing a clandestine drug
lab site. The chemicals and substances used in the manufacturing process can be caustic, explosive,
carcinogenic, poisonous, irritating, and/or flammable. Personnel participating in clandestine drug
laboratory investigations should have specialized training in the use of protective equipment and the
appropriate health and safety procedures.

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13.0 DRUG CHEMISTRY
Clandestine laboratory samples and reagents include highly corrosive acids, caustic bases, volatile
respiratory irritants, flammable liquids and many other dangerous substances. The appropriate use of
personal protection equipment is strongly recommended. In addition, samples must be packaged in a
safe manner for long term storage and transport.
The processing of clandestine drug laboratories also involves the sampling, removal, and proper disposal
of hazardous toxic chemicals. For forensic purposes, representative samples of all substances that
could be used for the synthesis of a controlled substance should be submitted to the lab. In the case of
multi-layer liquids, be sure the submitted sample contains sufficient material from both layers. Alternately,
layers may be submitted in separate containers, but be sure to appropriately label which container is the
top/bottom layer. Such information can be extremely important to the analyst.
In addition, photographs of the scene depicting any glassware along with any officer’s report should also
be submitted. If at any time the investigating personnel have questions about what should be sampled
on scene, it is encouraged that they contact their local forensic laboratory for assistance. The
transportation and disposal of all chemicals at the scene is regulated by state and federal environmental
protection agencies and as such, any questions regarding disposal procedure should be directed to
them.
13.9 Methamphetamine Quantification

Quantitative analysis on solid dose methamphetamine requires a separate request, usually by a federal
attorney, and is conducted when cases are being prosecuted at the federal level.

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14.0 ANONYMOUS ANALYSIS PROGRAM OF SUSPECTED CONTROLLED SUBSTANCES
14.0 ANONYMOUS ANALYSIS PROGRAM OF SUSPECTED CONTROLLED SUBSTANCES

The Oregon State Police Anonymous Analysis Program was established to assist parents/guardians in
the event they should find suspected drugs or other drug paraphernalia in their minor’s possession.
Anonymous Analysis is not performed at the request of employers.
14.1 Submission of Substances for Anonymous Analysis

Suspected drugs/paraphernalia can be submitted for controlled substance analysis by delivering the
item(s) to any local law enforcement agency or participating Oregon State Police Forensic Laboratory.
Samples submitted as part of the OSP Forensic Services Division's Anonymous Analysis Program will
not be used for criminal or civil charges and are not considered evidence.
Anonymous Analysis cases will be worked in a timely manner and will be dependent on staff availability
and caseloads. The findings will be reported verbally to the submitting agency or individual making the
request.
The report posted in LOIS will be titled Anonymous Analysis and will indicate the following: “The results
of anonymous analysis requests are reported verbally to the customer. Please contact the
agency/customer directly if you require information regarding this request.”
Anonymous Analysis samples will be destroyed by following the Division’s Policy for the destruction of a
controlled substance.

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15.0 TOXICOLOGY
15.0 TOXICOLOGY
During investigations when there is cause to believe that an individual may have been under the
influence of alcohol and/or drugs, efforts should be made to obtain blood and/or urine for toxicological
analysis.
15.1 Type of Sample

Alcohol - In cases where it is necessary to determine the level and effect of alcohol on the individual,
blood is the specimen of choice. Urine may be tested for the presence of alcohol, however it is not a
legally recognized testing medium for blood alcohol determinations and therefore a percentage of alcohol
will not be reported.
Drugs - In cases where the use of controlled substances or other drugs is in question, urine is the
specimen of choice.
The laboratory is also able to determine the alcohol concentration of unknown liquid samples.
Many drugs leave the blood very rapidly and may be difficult or impossible to detect unless the blood is
collected expeditiously. Generally, drugs are present in the urine in greater abundance than in blood,
and are therefore more readily detected. When there is a question as to which medium is best, collect
both blood and urine.
The Forensic Services Division is not able to test blood for the presence of drugs other than alcohol at
this time. If blood-drug analysis is required, the laboratory may be able to assist you in locating an
outside laboratory that is able to perform the work.
15.2 Triaging
If both urine and blood are submitted, the laboratory will test the blood for alcohol and the urine for drugs.
The laboratory may not test blood for alcohol concentration if a valid breath test has already been
administered, and may not test urine for drugs if the subject’s blood alcohol concentration is greater than
0.08% (w/v).
Cases that have pending criminal charges are prioritized higher than those that do not have pending
charges.

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15.0 TOXICOLOGY
15.3 Blood Alcohol
15.3.1 Collection, Packaging, and Storage

At least 4 milliliters of blood should be collected in a commercially available gray-top tube that contains
sodium fluoride and potassium oxalate or EDTA. Ideally, two 10 milliliter tubes will be collected at the
same time. Normally, the analyst will test one and leave the other unopened so it is available for
additional testing if desired.
When gray-stoppered tubes are not available, other types of blood samples may be submitted (serum or
plasma samples or tubes with other-colored stoppers).
The Forensic Services Division does not recommend the collection of multiple sequential blood draws.
Testimony given at the time of trial will be the same whether there is one blood draw or several.
Blood Alcohol Specimen Kits can be purchased for packaging and securing the blood tube. To order kits
go to the following link:
http://www.oregon.gov/osp/FORENSICS/Pages/KitOrders.aspx#Blood_Collection_Kits
A specimen labeling system must be employed which assures unequivocal matching of the specimen
with the person from whom it was collected. The tube(s) should be labeled with the individual’s name,
date, and time of the blood draw. If two or more vacutainer tubes are collected consecutively during the
same blood draw, they should be labeled with the same time.
Do not place evidence tape or other seals over the stopper of the vial, as the tape can obstruct the
information on the vial label, and can interfere with resealing of the tube. Instead, the packaging
containing the blood tube(s) should be securely sealed with evidence tape.
Care must be taken to maintain proper chain of custody. Blood should be submitted to the laboratory as
soon as reasonably possible, and should be refrigerated during any delay in submission. Blood
evidence returned to the submitting agency should be stored under refrigeration.
15.4 Urine
Only qualitative analysis (identification of drugs) is performed on urine specimens. Quantitation of a drug
in a urine sample is not performed because urine drug concentrations cannot be correlated to a
concentration of the drug in blood or to impairment. It should be noted that the mere presence of a drug
in the urine cannot, by itself, support a determination that the subject was under the influence of the drug
at a particular time. Likewise, the laboratory may be unable to detect drugs that are actively impairing a
person due to limitations of the analytical methods or because the drug has not yet been metabolized to
reach a sufficient concentration in the urine.

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15.0 TOXICOLOGY
If a Drug Recognition Evaluation (DRE) has been completed, please include a copy of the DRE face
sheet when submitting the evidence to the Laboratory.
If drug use is known or if the suspect has offered statements regarding drug use, it is recommended to
include that on the request form. Detection of some drugs (see below) requires specialized analytical
techniques beyond the routine analysis and it is useful for the analyst to know about those in advance of
testing.
15.4.1 Drugs Tested in Routine Toxicological Analysis

All urine samples generally undergo screening by EMIT (Enzyme Multiplied Immunoassay Technique)
and confirmation by Gas Chromatograph/Mass Spectrometer (GC/MS). The analyst looks for the
following types of drugs in a routine toxicology analysis:
 Opiates/Narcotic analgesics (e.g. morphine, Vicodin, codeine, etc.)

 Amphetamines/Stimulants (e.g. amphetamine, methamphetamine, MDMA, etc.)
 Barbiturates (e.g. butalbital, phenobarbital, etc.),
 Benzodiazepines (e.g. Valium, Klonopin, etc.)
 Marijuana metabolite
 Cocaine and its metabolites
 Methadone
 Other prescription drugs (e.g. Prozac, Soma, Ambien, etc.)
 Other potentially impairing over the counter drugs (antihistamines,
dextromethorphan, etc.)
The laboratory is currently unable to test for inhalants (e.g. paint thinner, “Dust Off,” etc.), synthetic
cannabinoids (e.g. “K2” or “Spice”) and LSD. If any of these types of drugs is suspected, collect a blood
sample, store it in a cool, dark place, and contact the laboratory for help in locating a lab to analyze the
sample.
If you suspect that lorazepam (Ativan), psilocin (mushrooms), or a date rape drug (e.g. GHB, etc.), was
used, this should be specifically noted on the Forensic Services Request (Form 49). These drugs may
not be detected in a routine toxicology analysis, and notice ensures that specialized detection methods
can be utilized if necessary.
15.4.2 Collection, Packaging, and Storage

Urine Collection Kits are provided by the Oregon State Police and should be available at all Intoxilyzer
locations. To order these kits go to the following link:
http://www.oregon.gov/osp/FORENSICS/Pages/KitOrders.aspx#Urine_Sample_Kits

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15.0 TOXICOLOGY
Urine samples should be collected in the plastic screw-top container provided in the kit. Be sure the lid is
tightly secured and label the container with the following information:
 The individual’s name (not just initials)

 The date of collection
 The time of collection
If there is a reason to suspect tampering, try to obtain another sample from the subject, and send both
samples to the laboratory.
Do not place evidence tape over the lid of the container, as this can obstruct the information on the label
and interfere with reclosing. Secure the urine cup in the plastic bag provided. It should be noted that
urine that leaks into the plastic bag will not be analyzed. The packaging containing the urine specimen
should be securely sealed.
Care must be taken to maintain proper chain of custody. Urine should be submitted to the laboratory as
soon as reasonably possible, and should be refrigerated during any delay in submission. Urine evidence
returned to the submitting agency should be stored in a secure freezer or in a refrigerator if freezer space
is limited.

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16.0 FIREARMS EVIDENCE

Firearm examinations compare marks or impressions which result when two objects make contact with
each other. These resulting impressions are characteristic of the “tool”, which is usually the harder of the
two objects. When a bullet, which is composed of relatively soft metals, travels through the harder barrel
of a firearm the barrel leaves markings on the bullet. These markings are unique and can often be
associated with a specific firearm. Fired cartridge cases can also be identified to a firearm in a similar
manner.
Some examples of analysis of physical evidence by Firearm Examiners include:
 Determining functionality/operability of a firearm.

 Determining if a firearm has been altered/modified into a machine gun.
 Determining the presence/functionality of a sound suppressor.
 Generating a list of possible firearm(s) based on the class characteristics found on fired bullets
and/or cartridge cases.
 Comparison of bullets, cartridge cases, or fired shot shells to determine if they were fired in a
particular firearm.
 Serial number restorations.
 Determination of distance from muzzle to target proximity based upon gunshot residues and/or
shot patterns.
 Manufacturer and type of ammunition.
 Examinations and conclusions regarding the identification of gunpowder.
For Trajectory analysis refer to the Crime Scene section in this manual.
16.1 Integrated Ballistics Identification System (IBIS)

IBIS is a system that captures and compares images of known test-fires and unknown fired cartridge
cases. These images are searched against a database. When similarities are observed, the evidence is
referred to a firearms examiner for confirmation. All firearms on the IBIS eligible list are automatically
entered at into IBIS, regardless of whether the examination is requested.
To request entry of evidence into IBIS, contact your local Forensic Laboratory for information on how to
submit. Firearms eligible for IBIS entry generally include the following:
 Centerfire and rimfire semiautomatic pistols

 7.62x39mm, 5.56x45mm/223 REM, and 22 LR caliber semiautomatic rifles
 Pump and semiautomatic shotguns

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16.2 Collection and Packaging of Firearms Evidence

The primary concerns when packaging firearms are safety and the preservation of the evidence including
blood, trace evidence, and latent prints that may be present.
 Never insert anything into the barrel of a firearm.

 Minimize handling because it is possible to recover latent prints from firearms and
ammunition.
 Do not remove cartridges from magazines, however, do remove the magazine. Ensure
magazines and recovered ammunition are both submitted with the firearm. The submission
of ammunition used with the firearm is essential to give accurate results in muzzle-to-target
proximity test.
 Absent special circumstances, recovered firearms and ammunition components should not be
physically marked in any manner (see exception listed below). Label the packaging instead.
 Mark the position of the cylinder on both sides of the top strap before opening the cylinder of
a revolver and make note of the position of fired and unfired cartridges in the cylinder. This is
so the position of the cylinder, as recovered, can be determined after the cylinder is opened.
Under Hammer
1 Rem 38 Spl (fired)

2 W-W 38 Spl (fired)
3 F-C 38 Spl (unfired)
4 F-C 38 Spl (unfired)
5 W-W 38 Spl (unfired)
Figure 5: Example of how to note the positions of

cartridges and cartridge cases in the cylinder of a
revolver.
 Store and transport firearms unloaded and rendered safe. If unable to do so, hand-deliver the
firearm to the laboratory and inform lab personnel immediately of the firearm’s condition.
 Any evidence with possible blood or body fluids should be air-dried, then packaged in paper
bags, envelopes, or cardboard boxes labeled as containing a biohazard. It is preferred that a
“BIOHAZARD” label is attached.

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 For firearms recovered from bodies of water, submit the firearm in a container that will keep it
submerged in the water it was recovered from. Do not dry the firearm out prior to submitting
to the lab.

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17.0 SERIAL NUMBER RESTORATION
17.0 SERIAL NUMBER RESTORATION

The obliteration of serial numbers and manufacturer’s marks is often done to prevent tracing ownership
of articles. The laboratory uses mechanical and chemical processes that may restore the original
marking in whole or part. Firearms, bicycles, motorcycles, chainsaws, boats, and cameras are all
evidence items where serial numbers have been restored.
17.1 Collection and Packaging of Evidence

Package the evidence in a manner that will protect the area where the serial number has been
obliterated. Contact the laboratory prior to delivering large items.

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18.0 TOOLMARK EVIDENCE
18.0 TOOL MARK EVIDENCE

Tool marks are impressions or marks produced when a tool comes into contact with an object; the tool is
generally the harder of the two objects. Physical contact between a tool and the surface of an object
produces marks not only characteristic of the type of tool used, but marks that may be unique to a single
tool.
In the absence of a suspect tool, tool mark impressions can be examined in an attempt to determine the
type of tool(s) that may have produced them. Examples of tools that may be encountered include:
hammers, screwdrivers, pry bars, knives, bolt cutters, pliers, tin snips, pipe wrenches, axes, and
hatchets.
Do not attempt to determine if a found tool fits in the tool mark. This may alter or obliterate the tool mark
and trace evidence may be lost or added.
18.1 Collection and Packaging of Evidence

The recovered tool should be carefully packaged to prevent the prying blade or cutting edges from
having contact with any other objects that may cause an alteration of the tool.
Send the whole object containing the tool marks to the laboratory. If this is not possible, photograph the
tool mark, then cut out the area with the tool mark or make a cast of the mark. Information about casting
material that is appropriate for tool marks may be obtained by contacting the laboratory.
Mark the cast or cut object with appropriate information indicating its orientation such as up/down,
inside/outside, and left/right directions. Package the object containing the tool mark in such a manner as
to prevent alteration or damage during shipment and storage.

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19.0 GUNPOWDER AND SHOT /PELLET P ATTERNS
19.0 GUNPOWDER AND SHOT/PELLET PATTERNS

When ammunition is fired, a mixture of partially burned\unburned gunpowder, and vaporized primer
compounds are expelled out of the firearm’s muzzle, in addition to the bullet, shot pellets and wad(s).
Examination of the evidence may reveal the following:

 Proximity, which is the distance from muzzle to target determined by gunshot residue and/or shot
pellet patterns
 Ammunition type
 Ammunition manufacturer
 Firearm condition
 Bullet or pellet entry angle
 Stippling or sooting around the entrance
19.1 Collection of Gunpowder Evidence

Submit the clothing or other object(s) that may have gunshot residue or bullet/pellet holes. Carefully
handle and package the evidence to avoid losing deposited gunpowder and/or other residue(s).
If possible, collect and submit ammunition of the same type used in the crime (e.g., ammunition from the
firearm’s magazine, unused ammunition from a box at the scene, etc.)

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20.0 ARSON AND FIRE DEBRIS

Many times it is difficult to ascertain whether a fire was accidental or arson. Flammable liquids readily
evaporate and thus arson evidence should be collected and packaged in an air tight container to prevent
loss by evaporation and possible contamination. Moisture is not a problem; do not dry arson evidence.
An arson investigator should be contacted with specific questions regarding the type of scene being
worked, what evidence collection is appropriate, and how to package/preserve evidence for submission
to the laboratory.
20.1 Arson Scene Indicators

 Multiple fires in unrelated areas of the fire scene
 Odor of petroleum products, paint solvents, alcohol, etc.
 Stains on floor or other material
 Evidence of explosions not due to heat (shattered glass)
 Rapid spread of fire not explainable by structure, weather, or other conditions
 Smoke not explainable by building materials
 Fire trails such as cloth or paper trails, burn trails on carpeting, or deep charring in hardwood
 Removal of household property and valuable items
 Evidence of another crime which the fire might conceal (items stolen, evidence of violence)
 Recent similar fires in the vicinity
20.2 Collection of Evidence

 Charred debris and related material from the origin where the accelerant was placed
 Igniting devices (fuses, rags, candles, etc.) including mechanical and electrical devices
 Samples of upholstery, drywall, plaster, wood, or other material that may have been penetrated
by flammable liquids
 Samples of soil that may have been penetrated by flammable liquids (freezing these samples
prevents degradation that can hamper analysis)
 Trace evidence possibly left by the arsonist such as hairs, shoe impressions, clothing fibers,
matches, etc.

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 Suspect clothing worn at time of crime, including shoes (nylon fire debris bags are the preferred
packaging for clothing items. Paper bags should be avoided.
 Liquids containing possible accelerants (for comparison to unknown samples) and containers that
may have been used to transport them
 Comparison sample - a sample of uncontaminated carpeting and/or padding, drywall, wood, etc.
should be collected and packaged separately
 Material used as a wick (shirt, sock, towel, etc.) from an incendiary device. This material may be
analyzed for DNA comparisons.
 Control samples – new, unused gauze, bags, swabs, etc.
20.3 Packaging of Evidence

 Use airtight containers. Unused, clean metal paint cans are preferred. Lined or unlined cans
work equally as well, but the lined cans will not rust through over time. Heat sealed bags
specifically manufactured for flammable evidence collection may also be used. When these bags
are used, submit an unused bag as a control. Contact the laboratory for information on where to
purchase these packaging supplies.
 Do not use paper bags (these allow volatile liquids to escape)
 Do not put gloves (used by the investigator while collecting evidence) in the container with the
evidence. Throw them away.
 Gauze pads can be used to soak up or collect residual liquid. Do not use cotton swabs, as they
do not collect enough material to test effectively. Using swabs to collect ignitable liquid residues
does not equate to collecting blood or biological material.
 Seal each collected item separately and securely.
 Mark all containers with appropriate identifiers.
 Document locations from which evidence samples were collected by notes, sketches, and/or
photographs.

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21.0 EXPLOSIVES
21.0 EXPLOSIVES
The Forensic Services Division accepts limited amounts of bulk explosives (less than 1 oz.) and post-
blast residues for laboratory analysis. A wide variety of chemicals and energetic materials may be
encountered in an explosives investigation scene. Analysis of some of these materials may be beyond
the capability of the OSP Forensics Services Division. It is recommended your agency consults with an
Explosives Analyst in the Portland Forensic Laboratory at (971) 673-8230. Evidence from a post-blast
explosives scene should be collected by individual with specialized training. It is recommended that you
call ATF or your agency’s bomb squad for the contact information.

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22.0 GLASS EVIDENCE
22.0 GLASS EVIDENCE

Glass is one of the more important types of physical evidence that is commonly overlooked. Glass is
frequently encountered in burglaries and hit and run cases, and glass fragments may be found adhering
to garments, hair, embedded in shoe soles, or may be transferred to other property belonging to the
victims and suspects.
Glass evidence cannot be individualized to a single source, however, there are some instances where
two fragments can be physically matched together and a common origin can be conclusively established.
Glass examinations may demonstrate the following:

 The presence and number of glass particles recovered from clothing or other surfaces.
 Whether or not fragments of recovered evidence glass are similar to glass from a known source
of broken glass
 The type of glass found (e.g., tempered glass, container glass, etc.)
 The direction of force (from inside or outside) used to break a window
 The order of shots fired into a window or windshield
Consider that large glass pieces may have latent fingerprints present and the broken edges of glass may
have other trace evidence present such as blood, hair, traces of bullets, or snagged fibers.
22.1 Collection and Packaging of Glass Standards

A comparison of evidence glass to a possible source requires the submission of glass standards. A
separate glass standard should be submitted for each broken glass item at the scene. For each glass
standard, submit at least ten fragments of broken glass from that item. These fragments should
represent the entire broken area of the item (i.e. taken from different areas of the broken item, if possible)
since physical properties may vary even within a single glass object.
For window glass standards, collect the glass that is still adhering to the window frame when possible.
Collecting glass standards from the ground increases the likelihood of introducing contaminant glass into
the standard. A second comparison standard may be collected from the ground and submitted
separately.
Vehicle windshields and some structural glass may be double paned, meaning that two different panes of
glass are present. Standards should be collected from multiple areas on both glass panes. Package the
standards from each pane separately, if possible.
Submit glass evidence in packaging that reduces the chance for further breakage.

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22.0 GLASS EVIDENCE
22.2 Collection and Packaging of Recovered Glass Evidence

 Package glass pieces from different locations into different containers, clearly marking the outside
packaging as to the location and description of the evidence.
 Collect and submit all glass pieces if you believe numerous glass pieces were from the same
object (e.g. a window, a bottle, vehicle headlights, etc.) and request the laboratory to attempt a
physical match.
 Label large glass pieces with orienting marks (e.g. up/down, inside/outside) when applicable.
 Collect and package a large glass piece in a rigid container such as a cardboard box. Protect the
broken or fractured edges of the glass from any additional damage or breakage.
 Depending on the size, small glass pieces can be packaged in envelopes, bags, paper folds, or
on Post-It notes. For very small glass particles, place the particle onto the adhesive of a Post-It
note and use a pen to circle around it. Fold the note in half, covering the glass particles, and then
put the labeled folded Post-It note in a paper envelope.
 Tape over any holes in the packaging through which small glass particles could be lost.
 Glass pieces that are slightly bigger can be packaged in envelopes or bags, and then secured in
a padded envelope to protect from further breakage or injury to those handling the evidence.
22.3 Clothing Items and Hair Combings

An individual who breaks a window with force or who is in contact with or nearby a breaking glass object
may have very small particles of glass on his/her clothing and hair.
Collect clothing items, taking care not to shake or handle the clothing more than necessary. Doing so
may dislodge these small glass particles. If the clothing is not stained with biological material (e.g. blood),
package in a paper bag carefully sealing all possible openings. If the clothing is stained with biological
material, allow the clothing to air-dry on a clean, dry surface and package in a paper bag. If this is not
possible, collect and submit to the lab ASAP.
To collect glass particles from hair, have the person stand or lean over a large clean sheet of
examination or butcher paper. Comb the hair to dislodge any particles so that they will fall onto the
paper. Fold the paper to enclose any debris from the hair and tape closed.
22.4 Vacuum Sweepings / Large Objects

In some cases, it is useful to screen large objects such as carpets, car mats/vehicle flooring, or
backpacks for glass particles. The entire object may be submitted, or a vacuum sweeping may be made
of these objects. Adhesive lifts are not recommended for collection of glass evidence from these
surfaces, as the lifts too quickly become saturated and lose the ability to pick up any glass present.

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23.0 PAINT EVIDENCE
23.0 PAINT EVIDENCE

Paints are used as surface coatings for a variety of surfaces such as vehicles, structures, safes, and
appliances. Many crimes, such as burglaries, hit and run vehicle investigations, and others involve
forceful activities that can result in the transfer of paint from the original source to another place, person,
or object. Paint transfer may also occur if wet paint is applied in the commission of a crime.
Paint evidence may be transferred from one object to another from contact or loose paint chips may be
collected at the crime scene for later comparison with a suspected source. Paint chips may also fracture
in such a way that a conclusive physical match can be made between the loose chips and the object they
came from.
Paints contain a multitude of components, many of which may be detected in very small samples such as
those encountered as evidence. Analysis and comparison of paint samples can be successful even when
they are extremely small in size.
Paint examinations can determine:

 Whether evidence paint sample(s) are similar to a paint standard
 The type of paint (vehicle, architectural, etc.)
 Lists of possible make and model of a vehicle by using Paint Data Query (PDQ), a computerized
database
 If a physical matches exists between paint coatings on two pieces of an object that was at one
time joined
23.1 Collecting and Packaging Paint Evidence

Paint comparison standards from a known object are required when the laboratory is required to perform
a paint comparison. Paint comparisons are performed on a variety of paint types including vehicle paints,
architectural paints, spray paints, cosmetic lacquers, etc.
A potential paint source may have one or more different kinds and colors of paint that are present (e.g.
vehicles), and the differences may only be apparent using microscopic or instrumental techniques.
Because of this, it is important to obtain paint standards that adequately represent all of the paint types
present on a potential source. If multiple body panels of a vehicle show damage, a paint standard
should be collected from each.
If paint transfer is loosely adhering to a surface, place a loose paper cover or Post-it note over the area
to protect it during packaging and transport. Do not place tape directly onto an area of paint transfer.
23.1.2 Procedures:

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23.0 PAINT EVIDENCE
Small Items (Easily Transported)
If an item that is a potential source or recipient of paint transfer is small enough to be easily packaged
and transported, then it should be submitted in its entirety (e.g. tools, keys, knives, spray paint cans,
etc.).
Large Items (Not Easily Transported)
If the potential paint source or paint transfer is on a large object or one not easily transported, such as a
vehicle part or a door from a residence, use the following method for sample collection:
1. Locate the area of damage. If paint transfer from one object to another is suspected, collect both
the area of suspected paint transfer and a nearby area of a paint standard that shows no paint
transfer. Collect a standard from an area as close to the point of damage as possible; on
vehicles, they should be from the same body part (i.e. hood, right front quarter panel, driver’s
door, etc.).
2. When contact between two painted surfaces is suspected, the possibility of cross-transfers must
be considered. Collect both objects, or collect areas showing paint transfer and standards from
both surfaces.
3. If broken paint edges are present, care should be taken to collect as much of the damaged edge
material as possible. The potential for a physical match may exist.
4. Use a clean razor blade, scalpel, or sharp knife to gently pry, carve, or chip the paint from the
surface down to the foundation or substrate.
5. Always clean collection tools between each use to avoid cross-contamination of one sample with
another.
6. Collect a total of about a nickel-sized amount of paint from each damaged area, when possible.
7. Place each paint sample into a paper fold or small paper envelope. Securely seal all possible
openings in the packaging, including seams and corners if necessary.
8. Securely seal and label the package(s) with a description of where the sample came from.
9. Continue to collect paint from each damaged area in the same manner, even if the object
appears uniformly painted. Also collect any samples that are visually different. Package and
label each area separately.
10. Paperwork should clearly document the locations of collection of any paint evidence to be
submitted to the laboratory.
CAUTIONS
 Do not package paint standards in the same envelope as recovered paint

evidence. This could allow cross contamination to occur.

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23.0 PAINT EVIDENCE
 Do not collect paint evidence on tape. The adhesive from the tape may interfere
with instrumental analysis of the paint. Post-it-type adhesive notes are
acceptable.
 Substantial variations in thickness and layer sequences over short distances can
exist across a painted surface. This is particularly true in architectural paint and
for vehicle paint where curves, corners, and edges are often impact points and
may have been subjected to previous damage, sanding, or over-painting. Known
paint samples should be collected from these areas, when recognized.
 Do not use plastic bags (including evidence bags) that have small holes in them.
Such bags are manufactured to allow excess air out; however, they are not
appropriate for trace evidence collection as small particles may be lost.
 Be aware that when a vehicle’s clear topcoat of paint is abraded, it may appear
white to the naked eye.
 When observing road debris, be on the lookout for paint chips and plastic parts
that may form a physical match with a damaged vehicle. Collect these items now
for later comparison to a suspect vehicle.
Micro-droplets of spray-paint on fibers

of a sleeve cuff

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23.0 PAINT EVIDENCE
Layers of original and

aftermarket paint from a
vehicle

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23.0 PAINT EVIDENCE
A physical match of two paint chips. Paint chip “A”

was recovered from the scene of a hit-and-run;
paint chip “B” was collected from the damaged
area of a suspect vehicle. Note the microscopic
scratches in the paint extending across the break.
Physical match of duct tape pieces. The lower piece

was recovered from the suspect’s possession; the
upper pieces were recovered from the crime scene.

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B

24.0 PLASTIC AND T APE EVIDENCE
24.0 PLASTIC AND TAPE EVIDENCE

Plastics are composed of polymers that are manufactured into a variety of different objects. Plastic
evidence that may be encountered includes broken vehicle reflectors, smears on clothing, and small
pieces of plastic from tapes and garbage bags.
Laboratory examination may be able to determine the type of plastic and whether or not it is similar to a
suspected source. For large, rigid plastic pieces (e.g. broken reflectors), the laboratory can attempt a
physical match.
Plastic fusion marks might be present on an individual’s clothing as a result of a high-energy impact with
a plastic component of a vehicle interior. Because a variety of different plastics may be present on the
interior of the same vehicle, it may be possible to establish the position of the individual within the vehicle
by comparing the plastic fusion mark to standards from the vehicle interior. Remember that a high-
energy impact can cause a plastic component in a vehicle interior to have fibers or a fabric impression
from the clothing, as well.
24.1 Collection of Plastic Evidence

Large, rigid plastic pieces may be collected and packaged into paper envelopes or bags, plastic bags, or
cardboard boxes.
Small pieces of pliable plastic evidence (e.g. tape pieces, piece of garbage bag, etc.) should be placed
into a paper envelope. Tape pieces that are adhesive should be affixed to a clear plastic sheet, not to a
piece of paper, prior to packaging.
Clothing with possible plastic fusion marks should be packaged separately into paper bags.

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25.0 MISCELLANEOUS TRACE EVIDENCE (INCLUDING POISONS)
25.0 MISCELLANEOUS TRACE EVIDENCE (INCLUDING POISON)

A number of forensic examinations do not fall neatly into one of the other laboratory categories. These
are assigned to Trace Miscellaneous. The most common types include analysis of food for suspected
poisons and of cosmetics and household products for harmful tampering.
It is extremely helpful to the examiner to receive a complete police report that details the suspected
poison or adulterant and any symptoms of the person(s) exposed. If the type of chemical is unknown,
the examiner will perform general screening tests that will include, as appropriate, controlled substances,
heavy metals, volatiles, and some pesticides/organics. It is not possible to screen evidence for every
possible poisonous substance.
In addition to the item in question, the lab may request the submission of a control sample. For instance,
were you to suspect that a beverage had been contaminated with antifreeze, you would submit the
beverage in question, plus an untainted sample of the same beverage. If a sample of the contaminant is
available, it should also be submitted.
Miscellaneous Trace examination also includes the general chemical identification of unknown solids,
liquids and gases, measurement of physical, chemical or elemental properties, and the comparison of
contents to product labeling. Again, case details should be submitted with the evidence in order that the
analysis can proceed efficiently.

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26.0 FIBER EVIDENCE
26.0 FIBER EVIDENCE

Textile fibers can be exchanged between individuals, between individuals and objects and between
objects. When fibers are associated with a possible source, such as fabric from the victim, suspect or
scene, it can help corroborate case information and/or provide a potential link between items, persons,
and/or places.
A fiber that is transferred and detected is also dependent on the nature and duration of the contact
between the suspect and the victim and/or scene or object and the persistence of the fibers after they
have been transferred.
Research has shown that with few exceptions, the largest quantity of fibers on an object is from the last
object to be in contact with it. Therefore, it is advantageous to consider collecting fiber evidence prior to
processing for other types of evidence. Caution should be used to prevent cross contamination of
evidence collected for fiber examinations, particularly when crime scene personnel will also be
responsible for collecting suspect or victim clothing items or for the processing of related scenes or
vehicles. Caution should also be used to not add fibers from your own environment and clothing to the
evidence (e.g. fleece coat, wool sweater).
Fiber examinations involve a comparison of samples from known and questioned sources to determine
whether they are consistent with having originated from the same source (e.g., carpet from a suspect’s
car compared with fibers removed from the victim’s clothing). Laboratory analysts examine various
physical, chemical, and microscopic properties of fibers when performing a comparison between
evidence fibers and a potential source. Common conclusions include statements regarding the similarity
or dissimilarity of the evidence fiber(s) to the possible source or standard. This comparison involves the
recognition and evaluation of class characteristics, which associate materials to a group, but never to a
single source. Conversely, individual characteristics allow the association between two or more items
with each other to the exclusion of all other items. For fiber examiners, this most often occurs when
pieces of fabric or cordage are physically matched, but rarely otherwise due to the mass production of
textiles in society.
Determining whether a textile has been cut, torn, or otherwise damaged may be a probative question for
a piece of evidence. It may be possible to distinguish the type of damage depending on the object used,
the textile type, and other factors.
26.1 Fiber Examinations

The following may be determined from fiber examinations:
 The type of fiber (e.g. natural or synthetic, animal fiber, glass fiber, etc.)

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26.0 FIBER EVIDENCE
 The possible product uses for the fiber (e.g. carpet fiber, clothing fiber, etc.)
 The degree of similarity between evidence fiber(s) and a fiber source
 Whether a textile has been cut, torn, or otherwise damaged and if a particular object could have
been used to create the defect
26.2 Collection of Fiber Evidence

Fiber evidence can be collected in a number of ways. Refer to “Fiber Collection Methods” table.
26.3 Collection of Fiber Standards

A fiber standard (or a possible source) is required when the laboratory is requested to perform a fiber
comparison. If the possible source can be packaged and transported to the laboratory with ease, submit
the entire object (e.g., clothing items, throw rugs, etc.).
Fiber Collection Methods
Method Description Packaging When to Use
Adhesive lifts Use fingerprint tape, cellophane Stick adhesive tapes For fibers you cannot
tape, or other clear adhesive onto a clear, see, or to be sure you
substrate and pat over the item. colorless plastic have not missed any.
Take care not to miss any areas sheet (e.g. Good on car seats,
or allow the tape to become transparency film). surfaces of clothing,
“overloaded.” Post-It notes may Place into a paper and other medium to
also be used for small areas. envelope or bag. large surfaces.
Fold Post-It notes in
half and place into a
paper envelope.
“Pick” Using your fingers or tweezers, Place the fiber into a For fibers you can see.
method carefully retrieve the fiber taking paper fold, in a folded
care not to pinch, crush, or stretch Post-It note, or paper
it. envelope.

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26.0 FIBER EVIDENCE
Vacuum Use a portable vacuum cleaner Carefully remove the For fibers you cannot
sweepings equipped with special traps filter trap, cover with see, or to be sure you
holding a piece of filter paper. the lid or cap, and have not missed any.
Lightly vacuum the surface of package in a paper Good on car seats,
interest. The goal is to collect fiber or plastic bag. sections of carpeting,
evidence that is on the surface of and other large
the object, not to clean it. surfaces.
Cautions
 Care should be taken to store adhesive lifting materials in such a way that they will not become
contaminated prior to use
 Use of tools, such as forceps and tweezers, may cause damage to the trace evidence.
 Clean any collection tools thoroughly between samples to prevent cross-contamination.
 Avoid serrated tools as they may be more difficult to clean thoroughly.
If the fiber source is believed to be from a large object or one not easily transported, such as car
upholstery or carpeting from a dwelling or vehicle, cut representative samples from various areas. Be
sure to collect samples from areas that are visually different (e.g. different colored areas, faded areas
due to sunlight, worn sections, etc.). Samples should be about 1x1 inch unless you see variations in the
item that would warrant a larger sample cutting.
Package the garment, object, or sample cuttings in paper envelopes or bags and clearly label with a
description of from where the standard came.
Do not package standards with evidence fibers, or allow them to be near or in contact with each other.
This could allow cross contamination to occur.
Adhesive lifts are NOT acceptable for collection of standards.
26.4 Fabric Damage

If it is necessary to determine if textile defects are the result of a cut or puncture, the item suspected of
creating the defect (e.g. knife, scissors, screwdriver, etc.) should also be submitted.

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27.0 HAIR EVIDENCE
27.0 HAIR EVIDENCE

Hair evidence can be obtained from the victim, the suspect, a crime scene, or from other evidence such
as clothing. Hair is valuable evidence, particularly in cases where the perpetrator is a stranger to the
victim or an environment.
27.1 Hair Examinations

The following may be determined with hair examinations:
 Similarity or dissimilarity between evidence hairs and a hair standards

 Determination of human or non-human origin
 Limited determination of animal species
 Determination of human body origin (e.g. head hair, pubic hair, etc.)
 Determination of classic racial characteristics
 Whether there are indications that a human hair was forcibly removed
 Alterations exhibited in a human head hair (e.g. bleached, dyed, etc.)
 Whether the root of a hair appears appropriate to attempt nuclear DNA analysis
Whole human head hairs and pubic hairs (where the root is present) generally have enough microscopic
features to allow for a meaningful comparison to a standard. Hairs from other parts of the body, or hair
fragments (where the root isn’t present), are typically not well suited for a comparison.
A hair examiner will examine and compare evidence hair to standards, basing their conclusion(s) on the
features of the hair samples. Common conclusions include statements regarding the similarity or
dissimilarity of the evidence hair to the standard; however, hair comparisons are not a means of
identification. An inadequate hair standard can affect the significance of hair comparison conclusions.
See below for guidance regarding collection of hair standards.
27.2 DNA Analysis of Hairs

Because nuclear DNA analysis of hairs is destructive and does not always end in a useable DNA result,
it is the policy of the Forensic Services Division to perform morphological hair comparisons prior to DNA
analysis when possible.
The hair examiner will evaluate the hair for nuclear or mitochondrial DNA. The nuclear DNA analysis
targets the root section of the hair. Mitochondrial DNA analysis of hairs can be performed on the root or
the shaft portion of the hair. The Forensic Services Division does not perform mitochondrial DNA
analysis, but can assist with the transfer of evidence to the FBI or a private laboratory when necessary.

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27.0 HAIR EVIDENCE
The amount of DNA in a hair is very small and therefore contamination may occur if precautions are not
taken. Do not touch hair evidence with your bare hands or contaminated gloves.
27.3 Collection of Hair Evidence

Hair evidence can be collected in a number of ways. The “Hair Collection Methods” table lists the various
methods and when they are appropriate.
Hair Collection Methods
Method Description Packaging When to Use
“Pick” Using your gloved fingers or tweezers, Place hair into a For hairs you can
method carefully retrieve the hair taking care paper fold, in a folded see.
not to pinch, crush, or stretch it. Post-It note, or paper
envelope.
Vacuum Use a portable vacuum cleaner Carefully remove For hairs you cannot
sweepings equipped with special traps holding a filter trap, cover with see, or to be sure
piece of filter paper. Lightly vacuum the lid or cap, and you have not missed
the surface of interest. The goal is to package in a paper any. Good on car
collect trace evidence that is on the or plastic bag. seats, sections of
surface of the object, not to clean the carpeting, and other
object. large surfaces.
Adhesive Use fingerprint tape, cellophane tape, Stick adhesive tapes For hairs you cannot
lifts* or other clear adhesive substrate and onto a clear, see, or to be sure
pat over the item. Take care not to colorless plastic you have not missed
miss any areas or allow the tape to sheet (e.g. any. Good on car
become “overloaded.” Post-It notes transparency film). seats, surfaces of
may also be used for small areas. Place into a paper clothing, and other
envelope or bag. medium to large
surfaces.
Scraping Use a clean spatula or long, flat tool to Carefully shake any For hairs you cannot
scrape the surfaces of an object onto trace evidence on the see. Works well on
a large, clean piece of paper. For this paper to the center clothing or other
to work well, the object should be and fold the paper. pliable objects.
hanging or held up vertically over the Seal the paper fold
paper, scraping downwards. and place into a
paper envelope or
bag.
Cautions
 Care should be taken to store adhesive lifting materials in such a way that they will not become
contaminated prior to use

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27.0 HAIR EVIDENCE
.
 Use of tools, such as forceps and tweezers, may cause damage to the trace evidence.
 Clean any collection tools thoroughly between samples to prevent cross-contamination.
 Avoid serrated tools as they may be more difficult to clean thoroughly.
27.4 Collection of Hair Standards

Because of the variation in characteristics among different hairs from the same body region of one
person, it is important to obtain a sufficient number of hairs in order to adequately represent the ranges
of all characteristics (e.g., color, length, etc.) present. If the ranges of characteristics are large, it
becomes necessary to obtain a large number of hairs.
Hairs should be collected from the head and pubic area if appropriate. Hairs from different regions have
different characteristics. A head hair standard cannot be used for comparison to pubic hair evidence.
Collect hair standards in the following manner:

 Obtain standards from all persons who might reasonably be considered a source of an unknown
hair (e.g., suspect, victim, and other individuals common to an environment).
 Obtain standards as soon as possible after the crime occurred. Hair naturally changes in its
characteristics over time because it is constantly growing. The standards should reflect the
individual’s hair as close to the date of the crime as possible.
 It is recommended that a known head hair sample consists of at least 24 hairs collected from 5
different areas of the scalp (center, front, back, and both sides). These hairs should be obtained
by both pulling and combing. The recommended procedure for obtaining combed hairs is to use a
clean, unused comb and repeatedly comb the hair over a large sheet of clean paper.
 If appropriate to the case, a pubic hair standard should consist of at least 24 hairs obtained by
both pulling and combing from different areas of the pubic region
 Gather all the hairs collected from a single body region and place into a paper fold or paper
envelope
 Seal and label the envelope with the individual’s name and the body region it was collected from.
 Do not package paper folds containing different individual’s hair in the same envelope, or
package hair standards with hair evidence. This could allow cross contamination to occur.
27.4.1 Secondary Standards

A secondary standard is not obtained from an individual directly, but from an object or location where the
individual is believed or known to have deposited hair (e.g., a hairbrush). Necessity should be the only
reason to obtain secondary hair standards vs. pulled/combed hair standards (e.g., missing person,
person buried/cremated before collection could occur).
Secondary hair standards may be acceptable if it can be demonstrated or documented that the hair

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27.0 HAIR EVIDENCE
collected from the object/location is unlikely to include hair(s) from other individuals. The acceptance of a
secondary standard for comparisons will be evaluated by the hair examiner on a case-by-case basis.

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28.0 IMPRESSION EVIDENCE

Shoe, tire and fabric impressions are routinely present at crime scenes and are frequently overlooked by
law enforcement personnel. Examination of these impressions may provide the investigator with valuable
leads such as the type, make/model, and approximate size of the footwear or tire. If properly
documented and collected, almost every impression left by a shoe or tire has value for forensic
comparison to a suspected source. Even when suspect footwear is not available, images of impressions
from scenes may be submitted to the laboratory for search in the SICAR (Shoeprint Image Capture and
Retrieval) database for make/model determination and comparison to impressions from other scenes.
The make/model of tire responsible for a tire impression may also be possible. Instructions for
submitting SICAR images via e-mail are listed below.
Impressions made by other objects (e.g., weapons) may also be encountered and may be
collected/documented using the methods described below.
28.1 Impression Evidence General Guidelines

 If impression evidence can be collected without damage, it should be photographed, packaged,
and submitted to the laboratory for examination under controlled conditions.
 Always include a scale when taking photographs of impressions to be used for examination. The
scale should be a two dimensional ruler and should be placed on the same plane as the
impression.
 Impressions in soil, sand, snow, or impressions which cannot be sent to the laboratory should be
photographed and collected via casting or lifting.
28.2 Photographing Impression Evidence

 Photographs of the evidence should always be taken before any attempt to collect it.
 Photograph the general scene that contains the impression evidence.
 Impression evidence should be photographed in an uncompressed format (e.g., TIFF or RAW).
Digital photography is acceptable; however, low resolution images may be of limited use in
comparisons.5
 Place the camera on a tripod with the camera directly over and perpendicular to the impression.
If the impression is on an angled surface adjust the camera to tilt it to a plane parallel to the
impression.t is important to avoid taking the photos at an angle to the impression. This can result
in the inability to accurately enlarge the images as needed for comparison.
 Adjust the camera height so the impression and scale fill the frame.
 Use overlapping exposures to record large impressions.
5 If using a film camera, select a camera with a large negative format such as 35mm or 4 x 5 inch.

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 Use side lighting at various angles and from various directions to illuminate tread design more
clearly. This may require shading the camera setup from high, bright sunlight with a makeshift
tent and access to a detachable flash unit.
Representation of the camera and

tripod setup for shoeprint photography

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Photograph of a shoeprint impression. Notice the shoeprint fills the frame, a scale is
present, and the camera is directly over the shoeprint, not at an angle.
28.3 Three-Dimensional Impressions

Three-dimensional impressions are those that have a significant depth, in addition to the length and
width of the impression. Commonly, they may be found in soil, sand, snow or other materials and the
detail within the impression may vary according to the substrate. Casting is an effective method of
collecting these types of impressions. Impressions should always be photographed prior to casting.
Photographs, however, are not considered a substitute for a cast. If a lengthy tire track is encountered,
an attempt should be made to cast a section at least three feet in length. Do not clean out debris that is
part of the impression or was present when the impression was made.
28.4 Casting Methods

Note: The methods described below are not applicable to casting of impressions in snow. Specialized
techniques are required for casting of snow impressions. Should the need arise to cast impressions in
snow, please contact the laboratory for assistance or advice regarding the casting of snow impressions.
Dental stone or die stone should be used to cast three-dimensional footwear and tire tread impressions.
Plaster of Paris is no longer recommended as an acceptable casting material. Dental stone can be
obtained from local dental supply houses or in pre-made ready to use kits at minimal cost.
If using bulk dental stone, two (2) pounds of dental stone may be placed into an 8x12 inch Ziploc plastic
bag; this amount will cast an average sized shoe impression. In preparation for use at crime scenes,
numerous two-pound bags can be prepared and stored.
When using a commercially prepared kit, follow manufacturer instructions for mixing.

VERSION 9

The following is the procedure for making a cast from a self-made (bulk) dental stone kit:
 Retrieve a two-pound bag, add about 10 ounces of water, and thoroughly mix in the closed bag.
The mixture should have the consistency of thin pancake batter. If needed, add more water or
dental stone to create the correct consistency.
 Metal forms may be placed around the impression to contain the casting mixture. These are less
critical with the advent of dental stone and other forensic casting materials6.
 Open the bag and, with the bag at ground level, carefully pour the mixture into or next to the
impressions, allowing it to gently flow into it. Fill the impression completely so that the mixture
overflows out of the impression.
 When the cast is firm but still soft, scratch identifying marks on the exposed surface or write
identifying marks with a permanent marker when the cast is dry.
 Allow the cast to dry for a minimum of twenty minutes in warm weather, longer in cold, wet
conditions.
 Carefully lift the cast. Do not try to clean the cast; cleaning will occur in the laboratory.
 Package the cast in a large brown paper bag or cardboard box (not plastic) and allow to dry for
an additional 48 hours.
28.5 Two Dimensional Impressions/Prints

A two-dimensional impression is one where there is no significant depth to the impression. A thin deposit
of dust, mud, blood, or other material from a shoe or tire onto a hard surface may create these
impressions.
 Floors, glass, desktops, doors, paper items, etc. can retain a dust or residue impression at a
scene. Some impressions may be clearly visible while others may be partially or totally latent.
 Latent dust shoeprints can often be located by turning out all lights and shining a flashlight across
the surface of interest at a low angle. For example, to search for latent shoeprints on a vinyl floor,
place the flashlight on the floor (or near it) and allow the long beam of light to shine across the
floor.
 Photograph the impressions before collecting.
 Attempt to enhance or lift the impression only if the entire item cannot be retrieved from the
scene and submitted to the laboratory.
 Dry dust and residue impressions may be lifted with an electrostatic lifting device, gelatin lift, or
adhesive lift. Contact the laboratory for purchasing information.
 Impressions made by wet or damp footwear can sometimes be enhanced by carefully dusting
with fingerprint powder. A small portion of the impression should be dusted first to test the
success of the powdering technique. The impression is then photographed and can be lifted with
a contrasting gelatin or adhesive-lifting material. Contact the laboratory for information on where
to purchase lifts.
6This was more critical when plaster of Paris was used as the casting material. The form was required because the
cast had to be about 2 inches thick to be reinforced properly. This is no longer a concern with dental stone and
other modern forensic casting materials.

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28.6 Collection and Packaging

 Whenever possible, collect the impressioned item and submit it to the laboratory.
 Protect the impressioned item so that the impression does not rub off.
 Package in a cardboard box or paper bag. Carefully securing the item to the bottom of a thin
cardboard box is a good way to protect flat impressioned items.
 Submit the photographs of the impression to the laboratory. These should be properly packaged
as evidence and submitted along with any casts or lifts.
28.7 Exemplars and Standards
28.7.1 Footwear Exemplars

There may be several people who have legitimately walked into a crime scene. These include first
responders, medical personnel, members of the crime scene team, funeral home or Medical Examiner’s
Office personnel, individuals from the District Attorney’s Office, etc. These people may leave shoeprints
at the scene and it can become difficult to distinguish evidentiary shoeprints from those that are artifacts
of the crime scene processing.
Exemplars may be collected from people entering the crime scene in a number of ways. A good time to
do this is have the person keeping the crime scene log require everybody entering the scene to give a
shoeprint exemplar before proceeding into it. This may be accomplished by:
 Taking a photograph of the shoe sole.

 Greasing the soles with a thin film of petroleum jelly and having the individual step onto a clean
piece of paper. Dust the grease print with fingerprint powder for visualization.
 Using a commercially available kit consisting of an ink pad with non-visible ink and foot-sized
pieces of paper for collection.
28.7.2 Footwear Standards

If a comparison is to be performed, footwear should be submitted to the laboratory as standards from all
individuals thought to have left evidence impressions.
28.8 SICAR submission instructions for emailed images

This is for make/model searches only. Comparisons must be submitted to the lab in-person on physical
media. These instructions apply for tire tracks too.
1) Fill out a Form 49. You can find one at
http://www.oregon.gov/osp/FORENSICS/docs/ospform49sept201.pdf
Make sure to fill in Agency Case #, Offense, Offense Date, Investigating Officer and their contact
information, and information regarding individuals (if known). Select “Footwear (SICAR)” in the
Requested Service section for tire tracks as well. Save the form 49 in the following format
Casenumber-SICAR.pdf (ex. 14-000546-SICAR.pdf). Fill out the other areas as shown below.

VERSION 9


VERSION 9

2) Prepare images: Select one or two images that best depicts a particular outsole design. If there
are multiple outsole designs, please send them all under one case number. Check the file sizes
of the images. If they are near 20 MB, the OSP email system may strip them from our email.
You may need to compress the image to reduce the file size. If you do this, please view before
sending to make sure it hasn’t become too pixelated. If you are having difficulties with a
particular image please contact the laboratory. Below is an example of impressions that depict
different parts of an outsole design. Given enough information we can narrow the search down to
a particular piece of footwear.

VERSION 9

3) Attach the images and Form 49 to an email and send to: [email protected]
Please indicate if there is other evidence that represents these impressions. Sometimes casts
and lifts show better detail of an outsole design. It may be necessary to request these items
before completing work on a case. Below is an example where the cast clearly shows the
lettering that is present in the outsole design. The lighting used in the image of the impression in
soil does not highlight the lettering sufficiently. Please indicate in the body of the email how many
images you are sending for a particular case.

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28.9 Tire Exemplars and Standard

It is highly recommended that tires be submitted to the laboratory for the making of tire exemplars and
comparison to unknown tire tracks. Whenever possible, tire exemplars should be made with the tire still
in place on the vehicle. If it is not possible to transport the vehicle or to collect the tire, please contact
your local laboratory for recommendations on how to proceed.

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29.0 COMPUTER EVIDENCE
29.0 COMPUTER EVIDENCE

The Forensic Services Division does not examine this type of evidence. The Federal Bureau of
Investigation (FBI) accepts this type of evidence at its regional laboratory in Portland, Oregon. Please
refer to the FBI’s NWRCFL website: http://www.nwrcfl.org/.

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30.0 HOW TO MAKE A P APERFOLD
30.0 HOW TO MAKE A PAPERFOLD
1. Fold a square piece of 2. Take one corner at the 3. Take the second corner at
paper into a triangle. If folded edge and bring the the folded edge and bring
using a rectangular piece of corner just past the center the corner just past the
paper, make the same fold point, keeping the two center point on the opposite
as above and then cut off folded edges together. side, keeping the two folded
the excess. edges together.
4. Bring all folded edges up 5. Take the top center point 6. Fold and tape seal along
together to the point where and tuck into the opening this opening.
the top of the paper starts to created by the folded
angle. edges.

VERSION 9

31.0 MANUAL REVISION HISTORY

Revisions Table to Physical Evidence Manual
Revision
Revision Author
Date #
0 Creation and Adoption of Physical Evidence Manual

May 2002
Removed references to Coos Bay Lab, added “gum seal”
1
February 2004 as ok for use as evidence sealer,
Re-write to comply with ISO requirements; changes in

January 2006 2
programs
October 20, Removed gum seal w/ dye indicator as an acceptable

3
2006 method of sealing evidence.
December 11, Updated lab area service map to show all Douglas Co. to
4
2006 Springfield.
Added information regarding touch evidence to 6.0;

added 25.0 Missing Persons and 26.0 Unidentified
October 26,
5 Remains. Deleted lab survey card from appendix. Slight
2007
changes/corrections/clarifications made to Fingerprints,
Firearms
January 31, Updated/added information to Missing Persons 25.0.

6
2008 Also changed contact info in 25.0 & 26.0 to UNT.
December 13, Complete revision of entire manual – Susan Hormann

7 S. Hormann
2011 Operations Manager
Technical
Leaders,
June 3, 2014 8 Complete revision of entire manual Odessa
Siegel and S.
Hormann

VERSION 9

Complete review of entire manual. Technical

September 10,  Updates to technical content throughout as Leaders,
9
2015 needed Operations
 13.2.3 Change in submission policy for pills, Manager
tablets, and capsules

VERSION 9

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PHYSICAL EVIDENCE MANUAL

OREGON STATE POLICE

PHYSICAL EVIDENCE M ANUAL

Issuing Authority: Effective:

PHYSICAL EVIDENCE M ANUAL

1.0 Forensic Services Division Mission Statement ................................................................................... 8

Issuing Authority: Effective:

PHYSICAL EVIDENCE M ANUAL

8.3 Family Reference Standard Collection Kit ................................................................................. 28

Issuing Authority: Effective:

PHYSICAL EVIDENCE M ANUAL

12.1 Collection, Packaging, and Submission of Evidence ............................................................... 43

Issuing Authority: Effective:

PHYSICAL EVIDENCE M ANUAL

19.0 Gunpowder and Shot/Pellet Patterns ............................................................................................. 62

Issuing Authority: Effective:

PHYSICAL EVIDENCE M ANUAL

28.0 Impression Evidence ...................................................................................................................... 82

Issuing Authority: Effective:

PHYSICAL EVIDENCE M ANUAL

1.0 FORENSIC SERVICES DIVISION MISSION STATEMENT

GOALS AND OBJECTIVES

FORENSIC SERVICES DIVISION GOALS

Issuing Authority: Effective:

PHYSICAL EVIDENCE M ANUAL

BREATH ALCOHOL PROGRAM (IMPLIED CONSENT UNIT)

CRIME SCENE/FIELD INVESTIGATIONS

Issuing Authority: Effective:

PHYSICAL EVIDENCE M ANUAL

Issuing Authority: Effective:

PHYSICAL EVIDENCE M ANUAL

OFFICE PHONE EMAIL

Issuing Authority: Effective:

PHYSICAL EVIDENCE M ANUAL

3.0 LABORATORY LOCATIONS AND SERVICES

Issuing Authority: Effective:

PHYSICAL EVIDENCE M ANUAL

Portland Metro Lab Central Point Lab Pendleton Lab

Issuing Authority: Effective:

PHYSICAL EVIDENCE M ANUAL

4.0 CASE COMMUNICATION

Issuing Authority: Effective:

PHYSICAL EVIDENCE M ANUAL

5.0 GENERAL EVIDENCE HANDLING

5.1 General Evidence Handling Guidelines

5.2 Evidence Collection

Issuing Authority: Effective:

PHYSICAL EVIDENCE M ANUAL

Packaging Type Uses

Metal cans Arson evidence

Sharps tube syringes

Issuing Authority: Effective:

PHYSICAL EVIDENCE M ANUAL

Evidence must be sealed before submission to the laboratory: