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A comparative study of physico-chemical,

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 Journal of Agricultural Science and Applications (J. Agric. Sci. Appl.)

A Comparative Study of Physico-Chemical,

Proximate Composition and Microbiological Muscle
Properties, in Two Species Shrimps of the Pacific
Tropical Coast
D. Puga-López1,2, J.T. Ponce-Palafox*1 ,G. Barba-Quintero3, M. Rosalía Torres-Herrera2, E. Romero-Beltrán4, J.L.
Arredondo Figueroa5
1
Universidad Autónoma de Nayarit, Posgrado en Ciencias Biológico-Agropecuarias- CENITT-Escuela Nacional de Ingeniería
Pesquera. Lab. Bioingeniería Costera. Tepic. Nayarit, México 63155.
2
INAPESCA, Centro Regional de Investigación Pesquera de Bahía Banderas Nayarit. Calle Tortuga No. 1, Cruz de
Huanacaxtle, Bahía de Banderas, Nayarit, México. 63732.
3
Instituto Tecnológico de Mazatlán, Col Urias, Mazatlán, Sinaloa, México. 63732.
4
INAPESCA, Centro Regional de Investigación Pesquera de Mazatlán. Av. Sábalo Cerritos S/N Estero El Yugo, Mazatlán
Sinaloa, México. 82110.
5
Universidad Autónoma de Aguascalientes, Posta Zootécnica, Departamento de Zootecnia, Centro de Ciencias Agropecuarias.
Km. 3 carretera Jesús María-La Posta, Jesús María Aguascalientes, México 20900.
Corresponding Author‘s E-mail: [email protected]

ABSTRACT

Physicochemical, proximate composition and microbiological analysis of white shrimp (Litopenaeus vannamei) and blue shrimp (L.
stylirostris) tissues were compared. The wild shrimp were collected off the coasts of Sinaloa, México (in front Navachiste Bay, Guasave) and
of Nayarit (in front Boca de Cuautla-Novilleros, Tecuala), Mexico. The results of our study suggested that meats of white and blue shrimp
are a good source of protein and lipids. The blue shrimp tend to have better protein content than white shrimp, and white shrimp tend to have
better lipid content than blue shrimp.
Keywords: protein; lipid; faecal coliform; l. vannamei; Litopenaeus stylirostris.

microbiological muscle properties of both shrimps has been

I. INTRODUCTION
reported. The main goal of this investigation was compare
Shrimp is one of the world’s most popular shellfish the properties of muscle of the white and blue shrimps in
(Turan et al., 2011). In Mexico the most important Pacific Sinaloa and Nayarit states, Mexico.
shrimp species are blue shrimp Litopenaeus stylirostris
(Stimpson, 1874), white shrimp Litopenaeus vannamei II. MATERIAL AND METHODS
(Boone, 1931), brown shrimp Farfantepenaeus
californiensis (Holmes, 1900), and crystal red shrimp A. Sample preparations
Farfantepenaeus brevirostris (Kingsley, 1978), which White shrimp and blue shrimp were collected on the
account 70% of domestic production of shrimp caught coast of Sinaloa, in front Navachiste Bay, Guasave
(García-Juárez et al., 2009). (25º19´37.36” N; 108°46´24.63” W) and Nayarit, in front
Shrimp meat is an excellent source of protein (Cao et al., Boca de Cuautla-Novilleros, Tecuala ( 22º15´33.08” N;
2009; Oksuz et al., 2009; Rødde et al., 2008). Its meat is low 105°45´48.98” W), Mexico and sampled by capturing
in fat, especially saturated fatty acids; contain high amounts shrimp trawls known as "changos" on boats with outboard
unsaturated fatty acids (HUFA) such as eicosapentaenoic motor at depths between 10 and 20 m. Total length (from tip
(20:5n3, EPA) and docosahexaenoic (22:6n3, DHA) acids of the rostrum to the tip of the telson along the mid-dorsal
(Puga-López et al., 2013), are excellent sources of essential line) and weight of each shrimp were measured. After
amino acids, and a good source of iron, zinc, copper, collection, each sample was placed in an air-tight
calcium and vitamin B12 (Roy et al., 2009). Nevertheless, polyethylene bag and immersed in plenty of crushed ice
the compositions can vary with the feed sources. until being transported to the laboratory (Laboratorio de
Additionally, proximate compositions, fatty acid profiles, Calidad de Agua y Alimentos del Centro Regional de
cholesterol contents and total carotenoid contents (Turan et Investigación Pesquera de Mazatlán, Sinaloa, México). The
al., 2011) of shrimps change seasonally. Both white shrimp muscle, hepatopancreas and shell were separated,
and blue shrimp have been accepted by consumers mainly respectively, were sampled shrimp and the others were kept
the large sizes > 35 g. However, little information regarding in a freezer at -18 °C. The shrimps were deveined and the
the physicochemical, proximate composition and edible portions were ground to obtain uniformity. All peeled

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 Journal of Agricultural Science and Applications (J. Agric. Sci. Appl.)

shrimp were composited, homogenized, and analysed. All 2. Shrimps had suitable moisture contents (72.95 to 73.91%).
analysis (physicochemical, proximate composition and Protein was found as the major constituent, indicating that
microbiological), were determined in triplicate. shrimp muscle can be a good source of amino acids. No
significant difference (P< 0,05) between the two species.
B. Physicochemical Analysis Nevertheless, protein levels showed a tendency to increase
The pH was determined in shrimp muscle using about 5 with blue shrimp. It was found that white shrimp had
to 10 g of sample homogenized in distilled water. After 5 significant (P<0,05) higher concentrations of lipid that blue
minutes at room temperature using a previously calibrated shrimp. The Sinaloa shrimp had significant higher
potentiometer reading was taken performed in triplicate. concentrations of ash than those obtained in the Nayarit
The % of chloride (NaCl) was obtained using 10 g of state.
sample, 0.1 N solution of silver nitrate, HNO3 and titrated TABLE I. PH, % CHLORINE AND TOTAL VOLATILE BASES NITROGEN
with 0.1 M solution of ammonium thiocyanate. TVB-N (TBV-N) COMPOSITION OF WHITE SHRIMP (L. VANNAMEI) AND BLUE SHRIMP
(total volatile basic nitrogen) was determined by distillation (L. STYLIROSTRIS) MUSCLE. MEANS FOLLOWED BY DIFFERENT LETTERS SHOW
technique Macro-Kjeldahl Method. Using 10 g of shrimp STATISTICAL DIFFERENCES.

muscle placed in a blender to homogenize. Analyses were Parameter/

WS WN SS SN
performed in triplicate. Source
pH 7.23±0.24 7.25±0.21 7.31±0.16 7.22±0.19
C. Proximate Composition Chlorides 0.72±0.02a 0.73±0.02a 0.66±0.03b 0.66±0.05b
TVB-N 34.85±1.66 33.79±2.03 35.75±2.13 34.44±1.97
Shrimp muscle were analysed for moisture and ash Volatile bases total nitrogen; WS = Sinaloa white shrimp; WN = Nayarit
contents were determined as described by Latimer (2012), white shrimp; SS = Sinaloa blue shrimp; SN = Nayarit blue shrimp. Units:
and protein content was determined by the Kjeldahl method. TBV-N, mg 100g-1; Chlorides, %.
These analyses were performed in triplicate. Crude lipid
content was performed by acid digestion prior to petroleum -1
TABLE II. PROXIMATE COMPOSITION (G 100G ) MEAT WHITE SHRIMP (L.
ether extraction (b.p. 40–60 ºC) in a Soxtec system (Latimer, VANNAMEI) AND BLUE SHIRIMP (L. STYLIROSTRIS).
2012). The values were expressed as % (wet weight basis).
Parameter/ WS WN SS SN
Source
D. Microbiological Analysis Moisture 73.91±1.06a 73.63±0.86a 72.95±0.88a 73.77±0.80a
Microbiological analysis was from each processing Protein 20.04±0.93a 20.10±0.52a 20.47±0.61a 20.46±0.48a
techniques performed according to the standard procedure Crude lipid 1.27±0.16a 1.42±0.17a 1.03±0.15b 0.95±0.13b
for the enumeration and identification of microorganisms Ash 2.26±1.66a 2.10±1.05b 2.30±0.71a 2.18±0.69b
(Cain et al., 2012). The most probable number (MPN)s WS = Sinaloa white shrimp; WN = Nayarit white shrimp; SS = Sinaloa
blue shrimp; SN = Nayarit blue shrimp.
technique was used to determine the level of faecal
coliforms. The samples were homogenized in a blender
using sterile physiological saline (0.85% NaCl solution).
Total coliform (TC) bacteria were assayed by the membrane C. Microbiological analysis
filtration technique with m-Endo agar (Difco) and incubated
for 24 h at 35°C. The standard M 7h FC membrane filtration Faecal coliforms were detectable in white and blue
method (1) was used to recover faecal coliforms (FC) at shrimps and their counts ranged from <3 to 86·MPN g-1 (Fig.
41.5°C. Peptone (0.1%) was used for all serial dilutions. All 1). Faecal coliform observed in different samples of Sinaloa
assays were done according to APHA (2012). state shrimp were between <3 to 10 MPN g-1, while in
Nayarit State shrimp the count of all the samples were <3 to
E. Statistical Analysis 86 MPN g-1. In about 40% of all samples was determined at
a concentration of <3 MPN g-1. The sanitary conditions of
Data were subjected to analysis of variance (ANOVA)
white and blue shrimp are best in the Sinaloa State.
and mean comparison was carried out using Tukey’s
multiple range test (Montgomeru, 2008). Statistical analyses
were performed using the Statistical Package for Social IV. DISCUSSION
Science (SPSS 11.0 for windows, SPSS Inc., ChicagoIL). The determination of TVB-N in shrimp and fresh
produce is used as an indicator of freshness and is a measure
III. RESULTS commonly used in trade (Cadun et al., 2008)]. This
determination quantifies the nitrogenous bases,
A. Physico-chemical Analysis trimethylamine, dimethylamine and ammonia produced
PH, % of chloride and TVB-N concentrations of shrimp during spoilage of shrimp (Ali, 2011). In this experiment the
are shown in Table 1. In general there were no differences nitrogenous bases of the thawed shrimp head were of 17.76
in the pH and TVB-N parameters in white and blue shrimp, mgN 100g-1 fresh head, concentration near the content of
only a significant (P<0,05) higher concentration of chlorides the anchovy (14 mgN 100g-1) maintained at 20-28 °C for 12
on white shrimp. The results of pH in the fresh muscle of all hours before process meal. In anchovy meal with a greater
shrimp were measured above 7.0. than 30 mg TVB-N 100g-1 in raw fish, the deterioration of
the raw material has a significant negative effect on
B. Proximate Composition consumption and growth of shrimp (Tsironi et al., 2009).
The results of proximate composition are presented in Table Biochemical alterations increase the pH of shrimp muscle

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 Journal of Agricultural Science and Applications (J. Agric. Sci. Appl.)

even under freezing conditions resulting in decreased coliforms in cultured P. indicus was 230 g, while Ali et al.
product quality over time. This increment of pH value can (2012) reported a total coliform count of >240 MPN g-1 in
be attributed to compounds accumulated from endogenous tropical shrimps. Ali et al. (2012) reported faecal coliform
and microbial enzymatic reactions Seabra et al., 2011). The counts of 11 to 240 MPN g-1, 2 to >2400 MPN g-1 and 0 to
results of pH in the fresh shrimp muscle were measured 1600 MPN g-1, respectively in freshly caught penaeid
above 7.0, this agrees with the findings by Reddy et al. shrimps. As the microbiological condition of the shrimp
(2012), Seabra et al. (2011) and Tsironi et al. (2009). studied this far below those reported for other species.
Greater ash content was noticeable in this work than in
V. CONCLUSIONS
shrimp from the farms in Songkhla and Suratthani provinces
(Rødde et al., 2008). The Sinaloa shrimp had significant The results of our study suggested that muscle of white
(P<0,05) higher concentrations of ash than those obtained in and blue shrimp are a good source of protein and lipids. The
the Nayarit state, these results may be attributed to the high differences in physicochemical compositions and
ash content of the diet, the high levels of chitin found in microbiological properties between groups might be
crustaceans consumed by the shrimp (Fall et al., 2012; associated with the origin and handling. Blue shrimp tend to
Khanafari et al., 2008). Proximate compositions (protein have better protein content than white shrimp, and white
and fat) of the edible part of shrimp are slightly different shrimp tend to have better lipid content than blue shrimp.
from that found by Rødde et al. (2008) for these species.
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