International Journal of Engineering and Techniques - Volume 4 Issue 3, May - June 2018

RESEARCH ARTICLE OPEN ACCESS

The Estimation of Mepivacaine Drug

rug in Mepivacaine
Hydrochloride
ydrochloride Injection by Reverse Phase High Performance
erformance
Liquid
iquid Chromatography

C. Hazarathaiah yadav1 ,Mr.

Dr.C. Mr. Mallibabu Alahari2
(Department off Chemistry, Vel Tech Rangarajan Dr.Sagunthala R&D institute of Science and Technology,
Technology Avadi, Chennai-
600062, India)

Abstract:
A simple, precise, rapid and accurate reverse phase HPLC method was developed for the estimation of Mepivacaine drug in
Mepivacaine Hydrochloride Injection. Develosil ODS-MG-5,
ODS 5, (150 x 4.6 mm), 5µm with mobile phase consisting of Potassium
dihydrogen phosphateate buffer and acetonitrile were mixed in the ratio 65:35 (v/v) respectively and degassed for about 10
minutes. Isocratic mode of chromatography technique was used. The flow rate was 1.5 mL/min and the eluents were monitored
at 220 nm. The retention time off the Mepivacaine peak is about 1.6 minutes. The detector response is linear in the
concentration of 10.060 to 30.180 mcg/mL, Y-intercept
Y is -726.11468,
726.11468, Residual sum of square is 72329401.8155and slop is
54607.2822.The percentage assay of Mepivacaine is 99.9 %. The method is validated by determining its accuracy, precision
and linearity.
Keywords — Mepivacaine Hydrochloride Injection, RP-HPLC
RP HPLC method and Isocratic mode of chromatography.
chromatography

localanaesthetics.
anaesthetics. It contains an amide linkage
I. BACKGROUND
between the aromatic nucleus and the amino group
Mepivacainehydrochloride is 2-
Piperidinecarboxamide, N-(2,6-dimethylphenyl)
dimethylphenyl)-1- Composition of Available Solutions In Water For Injection
methyl-,, monohydrochloride and has the following 1%
1%
1.5%
2%
structural formula. Multi Mult
Single Single 2%
ple iple
Dose Dose Single
Dose Dose
30 30 Dose 20
Dose Details 50 50
mL mL mL
mL mL
Vial Vial Vial
Vial Vial
mg/m mg/m mg/mL
mg/m mg/
L L
L mL
Mepivacaine
10 10 15 20 20
hydrochloride
Sodium
6.6 7 5.6 4.6 5
chloride
Potassium
Fig 1: Structure of Mepivacaine Hydrochloride 0.3 Nil 0.3 0.3 Nil
chloride
The molecular formula C 15 H 22 N 2 O • HCl Calcium
0.33 Nil 0.33 0.33 Nil
chloride
Mepivacaine hydrochloride
drochloride is a local anesthetic Methylparaben Nil 1 Nil Nil 1
available as sterile isotonic solutions (clear, *In Water for Injection
colorless) in concentrations of 1%, 1.5% and 2% Table 1: Available Water for Injections
for injection via local infiltration, peripheral nerve
block, and caudal and lumbar epidural blocks. The pH of the solution is adjusted between 4.5 and
Mepivacaine hydrochloride is related chemically 6.8 with sodium hydroxide or hydrochloric acid.
and pharmacologically to the amide-typetype

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 International Journal of Engineering and Techniques - Volume 4 Issue 3, May - June 2018
In the literature, several analytical techniques like A. Preparation of Diluent:
HPLC method, LCMS methods, Voltammetry, GC Water used as diluent for entire experiment
and UV spectrophotometric methods have been
B. Preparationof Standard solution: (20 mcg/mL)
reported for its determination in biological fluids
and formulations. The main purpose of the present A standard stock solution of the drug was
study was to establish relatively simple, sensitive prepared by 20.0 mg of Mepivacaine Hydrochloride working standard
and validated liquid chromatographic methods for was weighed and transferred into a 100 mL
the determination of Mepivacaine in Mepivacaine volumetric flask and diluted to volume with the
Hydrochloride Injection. The method was validated diluent and mixed well. 10.0 mL of the above
by determining its accuracy, precision and linearity standard stock solution was pipetted into 100 mL
as per ICH guidelines, Q2R1 [5-9] volumetric flask, diluted to volume with diluent and
mixed well.
II. OBJECTIVES C. Preparationof Sample solution: (20 mcg/mL)
The main objective of this study is to develop a 5.0 mL of the sample solution (1% of
simple, precise, linear and accurate reverse phase POLOCAINE-MPF solution) was taken and diluted
HPLC method for estimation of Mepivacainedrug with diluent up to 100 mL and mixed well. 4.0 mL
in Mepivacaine Hydrochloride Injection. of this solution was pipetted into 100 mL
A. Materials and Requirements volumetric flask, diluted to volume with diluent and
Commercially available Mepivacaine mixed well
Hydrochloride Injection of POLOCAINE-MPF. D. Linearity:
Acetonitrile, Potassium dihydrogen phosphate Linearity of Detector response was established by
anhydrous and HPLC grade water (Qualigens) were plotting a graph of concentration (in mcg/mL)
procured from market. Mepivacaine Hydrochloride versus peak area and determining correlation
Working standard was procured from the Centaur coefficient, y-intercept, slope, %y-intercept and
Laboratories Pvt. Ltd as a gift material residual sum of squares. Mepivacaine
B. Instruments Hydrochloride linearity solutions were prepared in
HPLC, Waters Alliance system with equipped the concentration range from about 50% (10
Diode Array Detector and automatic injector with mcg/mL) to about 150 % (30 mcg/mL) level of the
injection volume 50 µl. The HPLC data was target concentration (20 mcg/mL) and injected into
analyzed with Empower-3 Software, the HPLC system. The detector response was found
SARTOURIUS Analytical balance with model of to be linear and the results were found to be within
MSA225P-100-DA and FISHER SCIENTIFIC pH limit, refer table 2 and 3.
Concentration Peak
METER with model of XL15. % of Linearity Level
(mcg/mL) Area(AU)
III. METHODS 50 10.060 549906

The contents of mobile phase were mixture of 75 15.090 821778

0.02M Potassium Hydrogen Phosphate in Water 100 20.120 1100875
and Acetonitrile in the ratio of 65:35 (v/v)
125 25.351 1376991
respectively and degassed for about 10 minutes.
The run time was set about 5 minutes and the 150 30.180 1651299
column temperature was 40ºC. Prior to injection of Correlation coefficient,
0.999
Blank solution and drug solution, the column was NLT 0.997
equilibrated for at least 30 minutes with the mobile Y-intercept -726.11468
phase flowing through the system. The eluents were Slope 54607.2822
monitored at 220 nm
Residual sum of square 72329401.8155
Table 2: Linearity data

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 International Journal of Engineering and Techniques - Volume 4 Issue 3, May - June 2018
F. Accuracy:
Accuracy was determined by recovery studies of
Mepivacaine Hydrochloride, known amount of
Linearity of Mepivacaine
standard was added to the pre-analysed sample and
1800000
subjected to the proposed HPLC analysis. Results
1600000
of recovery study are shown in table 4. The study
Peak Response in AU

1400000
1200000 was done from about 50% (10 mcg/mL) to about
1000000 150 % (30 mcg/mL) level of the test concentration
800000
600000
(20 mcg/mL) at three different sample preparations
400000 for each level.
200000
0 Pr % RECOVERY
0.000 5.000 10.000 15.000 20.000 25.000 30.000 35.000 Recov ep
ery ar Mean (NLT Average
Concentration in mcg/mL
Level ati Inject Inject 95.0% & (NLT 95.0%
on ion-1 ion-2 NMT & NMT
s 105.0%) 105.0%)

Table 3: Linear Regression of Calibration curve 1 100.2 100.5 99.7

50% 2 99.8 100.8 99.1 100.0
Level 3 100.5 99.3 100.3
E. Precision: 1 100.0 100.9 100.7
The precision of test method was evaluated by 100% 100.5 2 100.6 100.5 100.1
Level 3 100.3 100.8 100.6
preparing six samples and analyzed as per the test
procedure. 50 µL of sample solution was injected 1 100.4 99.8 99.2
150% 2 100.1 100.8 100.6 100.1
into the injector of liquid chromatograph. The Level
retention time was found to be 1.6 minutes. The 3 100.6 100.5 99.3
amount of drug present per each sample preparation Table 5: Accuracy data
was calculated by comparing the peak area of the
sample solution with that of the standard solution. G. System Suitability:
The data are presented in table 4. Typical The system suitability tests were carried out on
chromatograms of Blank solution and Mepivacaine freshly prepared standard solution of Mepivacaine
Hydrochloride sample solution as shown in fig 2&3. Hydrochloride. The parameters studied to evaluate
the suitability of the system are given in table 6
% Assay of Ketorolac
Sample Preparations Tromethamine Observed Values
Analyst-1 Analyst-2 System suitability parameters
Analyst- 1 Analyst-2
Preparation – 1 99.8 100.6
USP Tailing factor for
Preparation – 2 100.2 100.1 Mepivacaine peak in standard
1.0 1.1
solution (NMT 2.0)
Preparation – 3 99.5 100.2
Relative Standard Deviation for
Preparation – 4 99.9 100.8 peak areas of Mepivacaine from
0.4 0.2
five replicate injections of
Preparation – 5 99.6 100.5 standard solution (NMT 2.0%)

Preparation – 6 100.5 100.1 USP Plate count for Mepivacaine

peak in standard solution (NLT 13000 11000
Individual Average 99.9 100.4 2000)
Individual (% RSD), NMT
0.4 0.3
2 Table 6: System suitability data
Overall Average 100.2

Overall % RSD, NMT 2 0.4

Table 4: Precision data

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 International Journal of Engineering and Techniques - Volume 4 Issue 3, May - June 2018
IV. RESULTS ACKNOWLEDGMENT
The results of the study showed that the proposed The author is grateful to the Management of
RP-HPLC method is simple, rapid, precise and VelTech University for their encouragement and
accurate, which is useful for the routine providing the necessary facilities to carry out the
determination of Mepivacaine drug in Mepivacaine research work.
Hydrochloride Injection. In the present developed
HPLC method, the standard and sample preparation REFERENCES
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Fig.2 Blank Chromatogram
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Fig.3Sample Chromatogram
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