Equipment Calibration/Maintenance: APPENDIX D - USFWS QA Program (5/04)

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The document discusses calibration and maintenance procedures for laboratory equipment used in fish health testing. Regular calibration and maintenance of equipment is important to ensure accurate and reliable results.

Equipment like autoclaves, balances, biosafety cabinets/hoods, centrifuges, electrophoresis units, ELISA readers, freezers, incubators, micro-pipettes, microscopes, pH meters, plate washers, thermal-cyclers, and timers require daily calibration according to the guidelines provided.

Calibration logs must document the date, results of calibration, and next calibration date. Maintenance logs must record the date, type of maintenance, and date for next maintenance. Repair logs need the date, type of repairs performed, and date repair was completed. All specific results and certifications must be filed.

APPENDIX D – USFWS QA Program (5/04)

EQUIPMENT CALIBRATION/MAINTENANCE

The following chart provides a guide for calibration of standard equipment used in the Fish Health Laboratory. All Intervals
suggested are subject to individual manufacturer and/or equipment manual.
EXTERNAL
CALIBRATION INTERNAL CHECK PARAMETERS TO INSTRUMENTS
EQUIPMENT INTERVAL INTERVAL CHECK REQUIRED
Temperature & pressure sustained Pressure gauge, safety valve,
Autoclaves Daily during operation temperature gauge, indicator strips
Balances 3 years Weekly Linearity, zero point, accuracy, Calibration reference weights
level
Biosafety Cabinets/hood 1 year Weekly Air flow, UV bulbs Anemometer, vacuum meter
Centrifuges During operation Balance, speed, temperature, timer Manufacturer provided
Electrophoresis Units Daily / as used Structural integrity, voltage Volt meter (power unit)
ELISA readers 1 year Monthly Lamp stability, optics, filters Calibration plate
Freezers Daily Visual, thermal stability Calibrated thermometer or
pyroprobe
Incubators Daily Temperature Calibrated thermometer
Daily / as used Dirt and damage, volume delivery 70% ethanol. If damaged, send for
Micro-pipettes 3 years accurate repair, graduated tips
Every three months Volume accuracy Gravimetric method
Micro-pipettor (automatic) Every three months Volume, accuracy Manufacturer’s procedure
Microscopes 3 years (cleaned) Daily / as used Alignment, bulbs Manufacturer procedure
pH meters Daily / as used Electrode drift or reduced response Check against two buffer solutions
Plate washer (automatic) Daily / as used Nozzles, hoses, vacuum, pH of Distilled water for flushing,
wash buffer instrument gauges, pH meter
Thermal-cyclers If needed Annually Block Temperature Probe or check with sample
replicate matrix
Thermometers (digital) 1 year 6 months Check at point in working range
against reference thermometer Certified reference thermometer
Thermometers (liquid in glass) 10 Years 6 months Check at point in working range Certified reference thermometer
against reference thermometer
Thermometers (reference) 10 years Before use Check at ice point
Timers 2 years Accuracy
Water baths Daily / as used Temperature and correlation with Calibrated thermometer
controls
Water Purification (deionizer) Daily / as used Conductivity and meter battery Conductivity meter, voltmeter
Water purification (glass Weekly / as used Conductivity, pH, hardness Conductivity and pH meter,
distillation) hardness test kit
APPENDIX D – USFWS QA Program (5/04)

EQUIPMENT LOGS
A separate file shall be maintained for each item of equipment, to contain updated information. The following is a
template set of required records to be kept for all equipment used for testing samples and calibration:

EQUIPMENT INFORMATION

ITEM:

INVENTORY NUMBER:

SERIAL NUMBER:

MANUFACTURER’S NAME AND CONTACT:

SOFTWARE USED (NAME)?

CUREENT LOCATION OF EQUIPMENT:

CURRENT STATUS: ¨ In Use

¨ In Repair ¨ Out of Service (see attached documentation for details)

Additional Items on File:


¨ Manufacturers Instructions
¨ Maintenance Plan
¨ Equipment Log (maintenance and calibration)
¨ Calibration Reports, Certificates, Adjustments made
¨ Documentation of Damage, Malfunction, modification or Repair
¨ Other:_________________________________________________

Signature Date
APPENDIX D – USFWS QA Program (5/04)

EQUIPMENT CALIBRATION LOG


Equipment Item/Inventory No.:
NEXT DATE FOR
DATE RESULTS OF CALIBRATION* INITIALS CALIBRATION

*All specific results and certifications must be documented and filed


APPENDIX D– USFWS QA Program (5/04)

EQUIPMENT MAINTENANCE LOG


Equipment Item/Inventory No.:
DATE FOR NEXT
DATE TYPE OF MAINTENENCE INITIALS MAINTENANCE

*All specific information must be documented and filed


APPENDIX D– USFWS QA Program (5/04)

EQUIPMENT REPAIR LOG


Equipment Item:
DATE REPAIR
DATE TYPE OF REPAIRS PERFORMED INITIALS COMPLETED

*All specific information must be documented and filed

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