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    Determining Glucose in Blood

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    Anggraini Nugroho P
    This document outlines the process for determining glucose levels in blood samples. It involves: 1) Preparing a blood sample by adding reagents and centrifuging to remove proteins. 2) Determining glucose content by adding the sample to copper alkalis, boiling, cooling, adding arsenomolybdat, and measuring absorbance at 660nm. 3) Creating a standard curve by measuring absorbance of glucose solutions at different concentrations and using it to calculate glucose levels in samples.

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    Determining Glucose in Blood

    Uploaded by

    Anggraini Nugroho P
    8 views2 pages
    This document outlines the process for determining glucose levels in blood samples. It involves: 1) Preparing a blood sample by adding reagents and centrifuging to remove proteins. 2) Determining glucose content by adding the sample to copper alkalis, boiling, cooling, adding arsenomolybdat, and measuring absorbance at 660nm. 3) Creating a standard curve by measuring absorbance of glucose solutions at different concentrations and using it to calculate glucose levels in samples.

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    This document outlines the process for determining glucose levels in blood samples. It involves: 1) Preparing a blood sample by adding reagents and centrifuging to remove proteins. 2) Determining glucose content by adding the sample to copper alkalis, boiling, cooling, adding arsenomolybdat, and measuring absorbance at 660nm. 3) Creating a standard curve by measuring absorbance of glucose solutions at different concentrations and using it to calculate glucose levels in samples.

    Copyright:

    © All Rights Reserved
    Download as DOC, PDF, TXT or read online from Scribd
    Download as doc, pdf, or txt
    8 views2 pages

    Determining Glucose in Blood

    Uploaded by

    Anggraini Nugroho P
    This document outlines the process for determining glucose levels in blood samples. It involves: 1) Preparing a blood sample by adding reagents and centrifuging to remove proteins. 2) Determining glucose content by adding the sample to copper alkalis, boiling, cooling, adding arsenomolybdat, and measuring absorbance at 660nm. 3) Creating a standard curve by measuring absorbance of glucose solutions at different concentrations and using it to calculate glucose levels in samples.

    Copyright:

    © All Rights Reserved
    Download as DOC, PDF, TXT or read online from Scribd
    Download as doc, pdf, or txt
    of 2

    Determining Glucose in blood

    1. Preparing sampel
    1 mL blood sample
    Poured into test tube
    Stirred
    added with 1 mL Ba(OH)2 0,3 N
    Mixed with mL ZnSO4.7H2O 5%
    Mixed
    Added2 mL (NH4)2SO4 0,5 N
    Stayed for 15 minutes
    Centrifuged 15 minutes in 3500 rpm
    Decantace

    Filtrate residue
    taken 1 mL
    added 5 drops burette
    changing color
    sample zero protein

    2. Determining glucose content in blood


    1 mL blood sample zero protein
    Poured into test tube
    Added 3 mL Cu alkalis
    Inserted in boiling water for 15 minutes
    Inserted in cooled water 10 minutes
    Added 3-5 drops 2 drops arsenomolybdat
    Mixed
    Read adsorbance with spectrotonic 20 in wave 660 nm

    Adsorbancy

    Making of standard Curve

    1 mL glucose 1 mL glucose 1 mL glucose 1 mL glucose


    [0,3 mg/ml] [0,5 mg/ml] [0,7 mg/ml] [0,9 mg/ml]

    poured into test tube


    Added 3 mL Cu alkalis
    Inserted into boiling water 15 minutes
    Inserted into cooled water for 10 minutes
    Added 2 drops arsenmollibdat
    Read adsorbance with spektrotonic 20 in wave 660 nm

    Adsorbancy
    Blanko solution

    1 mL aquades
    Poured into test tube
    Added 3 mL Cu alkalis
    Inserted into boiling water 15 minutes
    Inserted into cooled water for 10 minutes
    Added 2 drops arsenmollibdat
    Stirred
    Read adsorbance with spektrotonic 20 in wave 660 nm

    Adsorbancy

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