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    Test For Anthraquinone Glycosides

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    kaveesh_93
    This document describes two tests for detecting anthraquinone glycosides in plant extracts. The Bortrager's test involves boiling an extract with diluted sulfuric acid, separating with benzene or chloroform, and observing a pink or red color in the ammonia layer. The modified Borntrager's test detects C-glycosides by heating an extract with iron chloride and hydrochloric acid, extracting with an organic solvent, and observing a pinkish red color in the ammonia layer. Both tests identify anthraquinone glycosides through color changes induced by acids and bases.

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    Test For Anthraquinone Glycosides

    Uploaded by

    kaveesh_93
    2K views1 page
    This document describes two tests for detecting anthraquinone glycosides in plant extracts. The Bortrager's test involves boiling an extract with diluted sulfuric acid, separating with benzene or chloroform, and observing a pink or red color in the ammonia layer. The modified Borntrager's test detects C-glycosides by heating an extract with iron chloride and hydrochloric acid, extracting with an organic solvent, and observing a pinkish red color in the ammonia layer. Both tests identify anthraquinone glycosides through color changes induced by acids and bases.

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    TEST

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    Test for Anthraquinone Glycosides

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    This document describes two tests for detecting anthraquinone glycosides in plant extracts. The Bortrager's test involves boiling an extract with diluted sulfuric acid, separating with benzene or chloroform, and observing a pink or red color in the ammonia layer. The modified Borntrager's test detects C-glycosides by heating an extract with iron chloride and hydrochloric acid, extracting with an organic solvent, and observing a pinkish red color in the ammonia layer. Both tests identify anthraquinone glycosides through color changes induced by acids and bases.

    Copyright:

    © All Rights Reserved
    Download as DOCX, PDF, TXT or read online from Scribd
    Download as docx, pdf, or txt
    2K views1 page

    Test For Anthraquinone Glycosides

    Uploaded by

    kaveesh_93
    This document describes two tests for detecting anthraquinone glycosides in plant extracts. The Bortrager's test involves boiling an extract with diluted sulfuric acid, separating with benzene or chloroform, and observing a pink or red color in the ammonia layer. The modified Borntrager's test detects C-glycosides by heating an extract with iron chloride and hydrochloric acid, extracting with an organic solvent, and observing a pinkish red color in the ammonia layer. Both tests identify anthraquinone glycosides through color changes induced by acids and bases.

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    © All Rights Reserved
    Download as DOCX, PDF, TXT or read online from Scribd
    Download as docx, pdf, or txt
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    Test for Anthraquinone Glycosides

    Bortragers test for Anthraquinone glycosides: To 3 mL extract, add diluted H2SO4. Boil and filter.
    To cold filtrate, add equal volume benzene or chloroform. Shake well. Separate the organic
    solvent. Add ammonia. Ammoniacal layer turns pink or red.
    Modified Borntragers test for C-glycosides: To 5 mL extract, add 5 mL 5% FeCl3 and 5 mL diluted
    HCl. Heat for 5 minutes in boiling water bath. Cool and add benzene or an organic solvent. Shake
    well. Separate organic layer, add equal volume diluted ammonia. Ammoniacal layer shows
    pinkish red colour.

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