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    FAO Modified Starches

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    Sylviana
    This document provides specifications for trehalose, including its definition as a non-reducing disaccharide made from liquefied starch, its identification tests, purity standards, and assay method using liquid chromatography. The Joint FAO/WHO Expert Committee on Food Additives established an Acceptable Daily Intake of "not specified" for trehalose in 2000.

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    FAO Modified Starches

    Uploaded by

    Sylviana
    69 views2 pages
    This document provides specifications for trehalose, including its definition as a non-reducing disaccharide made from liquefied starch, its identification tests, purity standards, and assay method using liquid chromatography. The Joint FAO/WHO Expert Committee on Food Additives established an Acceptable Daily Intake of "not specified" for trehalose in 2000.

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    This document provides specifications for trehalose, including its definition as a non-reducing disaccharide made from liquefied starch, its identification tests, purity standards, and assay method using liquid chromatography. The Joint FAO/WHO Expert Committee on Food Additives established an Acceptable Daily Intake of "not specified" for trehalose in 2000.

    Copyright:

    © All Rights Reserved
    Download as PDF, TXT or read online from Scribd
    Download as pdf or txt
    69 views2 pages

    FAO Modified Starches

    Uploaded by

    Sylviana
    This document provides specifications for trehalose, including its definition as a non-reducing disaccharide made from liquefied starch, its identification tests, purity standards, and assay method using liquid chromatography. The Joint FAO/WHO Expert Committee on Food Additives established an Acceptable Daily Intake of "not specified" for trehalose in 2000.

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    © All Rights Reserved
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    of 2

    TREHALOSE

    New specifications prepared at the 55th JECFA (2000) and published in


    FNP 52 Add 8 (2000). An ADI "not specified" was established at the 55th
    JECFA (2000).

    SYNONYMS

    alpha,alpha-trehalose

    DEFINITION

    A non-reducing disaccharide consisting of two glucose moieties joined


    together by an alpha-1,1 glucosidic bond. It is made from liquefied starch by
    a multistep enzymatic process. The commercial product is the dihydrate.

    Chemical names

    alpha-D-glucopyranosyl-alpha-D-glucopyranoside, dihydrate

    C.A.S. number

    6138-23-4

    Chemical formula

    C12H22O11 2H2O

    Structural formula

    Formula weight

    378.33

    Assay

    Not less than 98% on the dry basis

    DESCRIPTION

    White or almost white crystals

    FUNCTIONAL USES Texturizer, stabilizer, humectant, sweetener


    CHARACTERISTICS
    IDENTIFICATION
    Solubility (Vol. 4)

    Soluble in water, slightly soluble in ethanol

    Specific rotation (Vol. 4) alpha (20, D): +199 (5% aqueous solution)
    Melting point (Vol. 4)

    97

    PURITY
    Loss on drying (Vol. 4)

    Not more than1.5% (60, 5h)

    Total ash (Vol. 4)

    Not more than 0.05%

    Lead (Vol. 4)

    Not more than 1mg/kg


    Determine using an atomic absorption technique appropriate to the
    specified level. The selection of sample size and method of sample
    preparation may be based on the principles of the method described in
    Volume 4, Instrumental Methods.

    METHOD OF
    ASSAY

    Determine by liquid chromatography using the following conditions.


    Sample preparation
    Weigh accurately about 3g of dry sample into a 100ml volumetric flask and
    add about 80 ml of deionized water. Bring sample to complete dissolution
    and dilute to mark with deionized water. Filter through a 0.45 micron filter.
    Preparation of standard solution
    Dissolve accurately weighed quantities of dry standard reference trehalose
    (available from Hayashibara Co., Ltd, 2-3 Shimoishii 1-chome, Okayama
    700, Japan) in water to obtain a solution having known concentration of
    about 30 mg of trehalose per ml.
    Apparatus
    Liquid chromatograph equipped with a refractive index detector and an
    integrating recorder.
    Conditions
    Column and packing : Shodex Ionpack KS-801 or equivalent
    -length : 300mm
    -diameter : 10mm
    -temperature : 50C
    Mobile phase : water
    Flow rate : 0.4 ml/min
    Injection volume : 8l
    Procedure
    Separately inject equal volumes of the sample solution and the standard
    solution into the chromatograph. Record the chromatograms and measure
    the response of the trehalose peak. Calculate the quantity in mg of
    trehalose in 1 ml of the sample solution by the following formula:
    % trehalose = 100 x (RU /RS) (Ws/WU)
    where
    RS = Peak area of trehalose in the standard preparation
    RU = Peak area of trehalose in the sample preparation
    WS = Weight in mg of trehalose in the standard preparation
    WU = Weight of dry sample in mg

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