NanoDrop 2000

NanoDrop Products
Thermo Scientific
NANODROP 2000 | 2000c
Thermo Scientific
NANODROP 8000
Thermo Scientific
NANODROP 3300
Spectrophotometer
Spectrophotometer
Fluorospectrometer
Part of Thermo Fisher Scientific
As the industry leader in micro-sample quantitation, Thermo Scientific NanoDrop

Products meet the needs of todays laboratory scientistinstruments that are smart,
simple and robust. We combine our extensive expertise in micro-sample analysis with
an in-depth understanding of real-life applications to deliver the latest in UV-Vis and
Fluorescence instrumentation.
Advantages of Our Innovative Technology Include

Small sample size: Our patented sample retention system allows you to analyze
samples as small as 0.5 l quickly and easily.
Fast and easy sample processing: Fastmeasure a sample in less than 5 seconds.
Easysimply pipette a sample onto the pedestal and measure, then wipe the
pedestal and move to the next sample.
Versatile measurement options: You can now measure a micro-volume sample directly
on a pedestal or use a cuvette when neededall within the same instrument.
Small footprint: We understand the need to maximize space in a laboratory, so our
instruments are ultra-compact.
Pipetting 1l onto pedestal
Measuring 1l sample
No dilutions: Even high concentration samples can be measured without dilutions.

Unsurpassed technical support: Our customer service experts are life science
specialists with extensive experience in micro-volume analysis, so we are able to
help with specific applications to ensure optimal instrument use.
Innovative software: Our instruments are both powerful and easy to use, and as
always free software updates are available for downloading on our website.e
NANODROP 2000 | 2000c

Spectrophotometer
Thermo Scientific NanoDrop 2000 Spectrophotometer

Everything You Loved in the Thermo Scientific NanoDrop 1000, Plus More
The New Thermo Scientific NanoDrop 2000 delivers the same high-quality
performance youve come to expect from our full-spectrum UV-Vis instruments,
and it provides many enhancements:
Fast measurement time of less than five seconds
New, innovative software that allows you to create custom methods and provides
flexible options to design reports and export your data
Improved capability for proteins with low wavelength absorbance, such as peptides
at 205 nm
Sample volumes as small as 0.5 l, which is ideal for precious high concentration samples
Higher concentration measurement capability up to 15,000 ng/l (dsDNA), which
eliminates the need to dilute highly concentrated samples
Thermo Scientific NanoDrop 2000c Spectrophotometer

The Best of Both Worlds in One Spectrophotometer
The Thermo Scientific NanoDrop 2000c does everything the
NanoDrop 2000 does, plus more. With its unique, patentpending technology, the NanoDrop 2000c combines microvolume pedestal technology and cuvette capability in a single
instrument. This innovative technology allows you the flexibility
to choose the best measuring technique for your samples:
Innovative technology that makes this the only UV-Vis
spectrophotometer your lab will ever need
Expanded measurement options for all types of samples
you choose the measuring option right for your sample:
cuvette or pedestal
Broader concentration range for measuring very low
concentrations and very high concentrations
Cuvette capability allows for kinetics (time or time/temperature
studies) and cell culture (OD 600) measurements
NANODROP 8000
Spectrophotometer
Thermo Scientific NanoDrop 8000 Spectrophotometer

Process More Samples in Less Time
With the Thermo Scientific NanoDrop 8000, you can measure more samples in less
time without sacrificing the reliability and easy-to-use technology of the single
sample model. The NanoDrop 8000 spectrophotometer takes full-spectrum UV-Vis
absorbance measurements of up to eight samples simultaneously. Using an eightchannel pipette to dispense samples on a linear array of pedestals, you can easily
measure 96 samples in less than six minutes. This instrument offers:
Improved productivity with capability of analyzing up to eight 1 l samples at
one time
New, innovative software for creating custom methods with unit selection, oligo
calculator and flexible options to design reports and export data
Increased efficiency with the Sample Position Illuminator, which reduces error by
keeping track of the samples to be measured
High throughput for environments such as biorepositories, genotyping facilities
and quality control labs
Improved productivity for busy labs where multiple users currently use the single
sample model
Sample Position Illuminator
Multi-Sample Loading
Multi-Sample Measurement
NANODROP 3300
Fluorospectrometer
Thermo Scientific NanoDrop 3300

Fluorospectrometer
Full-Spectrum Fluorescent Analysis
Using our patented technology, the Thermo Scientific NanoDrop 3300 Fluorospectrometer
performs broad spectrum fluorescent analysis in a versatile, high-performance
instrument. The NanoDrop 3300 significantly lowers the mass detection limit more
than an order of magnitude, compared to conventional fluorometers. The NanoDrop
3300 is a powerful instrument that offers many benefits:
Sample size as small as 1l, which conserves precious samples for other
applications and testing
Wide excitation range without the need to change filters or use of a monochromator
Multiple emission profiles from a single sample by exciting multiple fluorophores
simultaneously due to broad excitation wavelength range
Easy to use even for those with limited fluorescence expertise
FRET
Real-time PCR
NanoDrop 3300 Fluorospectrometer Applications
UV LED max = 365 nm; equipped with cut filter that eliminates excitation above 400 nm
Example Applications:
GFP wt
Hoechst 33258
4-MU
Q Dots various
Fluoraldehyde OPA
Fluorescamine
FRET
Small Molecule
Crystallization
365 nm
UV LED
Sequencing
Histocompatibility
Blue LED max = 470 nm

GFP wt
eGFP
FITC-FAM
Alexa 488
PicoGreen
RiboGreen
Alexa 555
B-Phycoerythrin
Q Dot various
SybrGreen
SybrGold
FRET
470 nm
Blue LED
Microarrays
DNA RNA
Proteins
White LED range = 460 650 nm; uses virtual filtering

Cy3, Alexa 555
Alexa 568
Cy5, Alexa 647
Sulforhodamine 101
5-CMTR
Q Dots various
TET
Hex
Molecular beacons
460 nm
White LED
650 nm
Antibodies
Genotyping
Microgenomics
Specifications
NanoDrop 2000 | 2000c
NanoDrop 8000
NanoDrop 3300
Spectrophotometer
Fluorospectrometer
(pedestal)
Instrument Type
Minimum Sample Size
Sample Number
Path Length
Light Source(s)
Excitation Maxima
of LEDs
Detector Type
Wavelength Range
Wavelength Accuracy
Spectral Resolution
Absorbance Precision
Absorbance Accuracy
Absorbance Range
Fluorescence Range
Detection Limit
Maximum
Concentration
Measurement Time
Footprint
Weight
Sample Pedestal
Material of
Construction
Operating Voltage
Spectrophotometer
0.5 l
1 l
1
up to 8
1 mm (auto-ranging to 0.05 mm) 1 mm (auto-ranging to 0.2 mm)
Xenon flash lamp
N/A
2048 - element linear

silicon CCD array
190 840 nm
220 750 nm
1nm
< 1.8 nm (FWHM at Hg 253.7 nm) 3 nm (FWHM at Hg 546 nm)
0.002 (1 mm path)
0.003 (1 mm path)
2% (at 0.76 at 257 nm)
0.02 300 (10 mm equivalent) 0.02 75 (10 mm equivalent)
1 l
1
N/A
3 light emitting diodes (LEDs)

UV: 365 nm, Blue: 470 nm,
White: 460 650 nm
2048 - element linear
silicon CCD array
400 750 nm
1 nm
8 nm (FWHM at Hg 546 nm)
< 5% CV (10 nM fluorescein)
N/A
N/A
2 ng/l (dsDNA)
2.5 ng/l (dsDNA)
> 4 logs fluorescein

< 1 fmol fluorescein
15,000 ng/l (dsDNA)
3,700 ng/l (dsDNA)
N/A
< 5 seconds
14 x 20 cm
2.0 kg
< 20 seconds
24 x 32 cm
3.4 kg
2 10 seconds
14 x 20 cm
1.5 kg
N/A
303 stainless steel and

quartz fiber
303 stainless steel and

quartz fiber
12 vdc
5 vdc (all power supplied

by USB port)
Operating Power
1218 W (max 30 W)
Consumption
Standby Power
5W
Consumption
Software Compatibility Windows XP and Vista (32 bit)

Specifications NanoDrop 2000c cuvette:
Beam Height: 8.5 mm
Heating: 37 0.5C
Stirrer: 150 850 rpm
Path Length: 10, 5, 2, 1 mm
Absorbance Range: 0.002 1.5 A
Detection Limit: 0.4 ng/l (dsDNA)
Maximum Concentration: 750 ng/l (dsDNA)
Measurement Time: < 3 seconds
Weight: 2.1 kg
2W
30 W
1W
6W
Windows 2000, XP and Vista (32 bit)
All NanoDrop instruments are approved to CE

and UL/CSA standards.
www.nanodrop.com | 302-479-7707
2009 Thermo Fisher Scientific Inc. All rights reserved. All trademarks are the property of Thermo Fisher Scientific Inc. and its subsidiaries.

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What are the advantages of Thermo Scientific NanoDrop products?

What are the advantages of Thermo Scientific NanoDrop products?

What are some of the features of the NanoDrop 2000 spectrophotometer?

What are some of the features of the NanoDrop 2000 spectrophotometer?

NanoDrop Products

Part of Thermo Fisher Scientific

As the industry leader in micro-sample quantitation, Thermo Scientific NanoDrop

Advantages of Our Innovative Technology Include

No dilutions: Even high concentration samples can be measured without dilutions.

NANODROP 2000 | 2000c

Thermo Scientific NanoDrop 2000 Spectrophotometer

Thermo Scientific NanoDrop 2000c Spectrophotometer

Thermo Scientific NanoDrop 8000 Spectrophotometer

Sample Position Illuminator

Thermo Scientific NanoDrop 3300

Blue LED max = 470 nm

White LED range = 460 650 nm; uses virtual filtering

2048 - element linear

3 light emitting diodes (LEDs)

2.5 ng/l (dsDNA)

> 4 logs fluorescein

15,000 ng/l (dsDNA)

3,700 ng/l (dsDNA)

303 stainless steel and

303 stainless steel and

5 vdc (all power supplied

Software Compatibility Windows XP and Vista (32 bit)

Windows 2000, XP and Vista (32 bit)

All NanoDrop instruments are approved to CE

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