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Mitosis

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Cell Continuity -is the unbroken succession of cells since life evolved 3.8 billion years ago.

New cells can only be produced by cell division.


New cells are needed for
o
reproduction,
o
formation of multicellular organisms, and
o
cell replacement.

Cell Cycle
There are three stages of the cell cycle:
1. Interphase -The cell grows, proteins are made, the
number of organelles increases, DNA
replication.
2. Division of the Nucleus:

Mitosis: two daughter nuclei, genetically


identical the original nucleus, are formed.

Meiosis: formation of four haploid genetically


different daughter nuclei from the
original diploid nucleus.
3. Cytokinesis:
The cytoplasm divides between the new daughter nuclei.
Therefore each nucleus with its allocation of cytoplasm
becomes a new cell.
Mitosis
All nuclei can undergo mitosis.
Haploid (n): one set of chromosomes is present in the nuclei (i.e. only one of each different
chromosome is present.)
Diploid (2n): two sets of chromosomes are present in the nuclei (i.e. two of each different
chromosome are present.)
Role of Mitosis
Formation of a multicellular organism.
Asexual reproduction e.g. Amoeba, yeast and vegetative reproduction of plant.
Cell replacement and regeneration.
Faithful copying of genes and their transfer to the next generation of nuclei or cells.
Maintains the correct chromosome number of somatic (= normal body) cells.

Stages of Mitosis (Interphase); Metaphase, Anaphase, Telophase. (Interphase)


(Mnemonic I Pee MAT)
Prophase
chromatin condenses forming chromosomes
chromosomes shorten, thicken, coil, dehydrate
each chromosome consists of two identical sister
chromatids
o connected at the centromere
centriole divides forming the spindle fibres
nuclear envelope breaks down at the end of

prophase
Metaphase
chromosomes placed individually along the equator of the cell
each chromosome is connected by two sets of spindle fibres attached to
the centromeres

Anaphase
separation of sister chromatids, now termed chromosomes
the centromeres spit when the spindle fibres shorten
shortening results in two identical sets of chromosomes at opposite sides
of the cell
Telophase
each chromosome group becomes a nucleus when a nuclear envelope is
formed around it
the chromosomes uncoil to chromatin
o chromosomes lengthen, uncoil, rehydrate

Definitions
Chromatin: DNA in its normal, i.e. functional state condenses to form chromosomes.
Chromosomes:
condensed chromatin showing up as a group of short thread-like structures
only visible with the light microscope in nuclei during mitosis and meiosis
each chromosome carries a specific set of genes in linear order at particular loci (= sites)
Chromatid:
often described as half a chromosome
it is one of two threads of condensed chromatin forming one chromosome
the two threads are connected together at the centromere after DNA replication (S phase)
they exist only during prophase and metaphase (i.e. when chromosomes are X-shaped)
Centromere: a non-DNA region of a chromosome where sister chromatids are held together and
spindle fibres attach.

Meiosis
Meiosis: the division of a diploid nucleus to form four haploid genetically different daughter nuclei.
Role of Meiosis
Increases genetic variation in the population role in evolution
Makes sexual reproduction possible
Gamete formation in animals, fungi, protoctista e.g. Man
Site of Meiosis
Animals
testis - forming sperm, the haploid male gametes;
ovary - forming egg cells, the haploid female gametes.
Flowering plants:
anther of the stamen - forming the haploid male spores (microspores),
ovule of ovary - forming the haploid female spores (megaspores).

IHW March 2005

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