Cell Cycle and Mitosis
Cell Cycle and Mitosis
Cell Cycle and Mitosis
Lectures by
Erin Barley
Kathleen Fitzpatrick
INTERPHASE
G1 S
(DNA synthesis)
G2
G1 checkpoint
Cleavage
furrow
100 m
Figure 12.UN01
P
G1 S
Cytokinesis
Mitosis G2
Prophase
Telophase and
Cytokinesis
Prometaphase
Anaphase
Metaphase
Meiosis I
Figure 12.1
• In unicellular organisms, division of one cell
reproduces the entire organism
• Multicellular organisms depend on cell division for
– Development from a fertilized cell
– Growth
– Repair
• Cell division is an integral part of the cell cycle,
the life of a cell from formation to its own division
200 m
(b) Growth and
development
20 m
(c) Tissue renewal
Figure 12.2a
200 m
(b) Growth and
development
Figure 12.2c
20 m
(c) Tissue renewal
Concept 12.1: Most cell division results in
genetically identical daughter cells
• Most cell division results in daughter cells with
identical genetic information, DNA
• The exception is meiosis, a special type of division
that can produce sperm and egg cells
20 m
• Eukaryotic chromosomes consist of chromatin, a
complex of DNA and protein that condenses
during cell division
• Every eukaryotic species has a characteristic
number of chromosomes in each cell nucleus
• Somatic cells (nonreproductive cells) have two
sets of chromosomes
• Gametes (reproductive cells: sperm and eggs)
have half as many chromosomes as somatic cells
Sister
chromatids
Centromere 0.5 m
• During cell division, the two sister chromatids of
each duplicated chromosome separate and move
into two nuclei
• Once separate, the chromatids are called
chromosomes
Chromosome
arm
Figure 12.5-2
Chromosomal
Chromosomes DNA molecules
1 Centromere
Chromosome
arm
Chromosome duplication
(including DNA replication)
and condensation
2
Sister
chromatids
Figure 12.5-3
Chromosomal
Chromosomes DNA molecules
1 Centromere
Chromosome
arm
Chromosome duplication
(including DNA replication)
and condensation
2
Sister
chromatids
Separation of sister
chromatids into
two chromosomes
3
• Eukaryotic cell division consists of
– Mitosis, the division of the genetic material in the
nucleus
– Cytokinesis, the division of the cytoplasm
• Gametes are produced by a variation of cell
division called meiosis
• Meiosis yields nonidentical daughter cells that
have only one set of chromosomes, half as many
as the parent cell
10 m
G2 of Interphase Prophase Prometaphase Metaphase Anaphase Telophase and Cytokinesis
Centrosomes Chromatin Fragments Nonkinetochore
(with centriole pairs) (duplicated) Early mitotic Aster of nuclear microtubules Metaphase Cleavage Nucleolus
spindle Centromere envelope plate furrow forming
Plasma
Nucleolus Nuclear membrane Chromosome, consisting Kinetochore Kinetochore Nuclear
envelope of two sister chromatids microtubule Spindle Centrosome at Daughter envelope
one spindle pole chromosomes forming
Figure 12.7a
Plasma
Nucleolus membrane Kinetochore Kinetochore
Chromosome, consisting
Nuclear of two sister chromatids microtubule
envelope
Figure 12.7b
Nuclear
Spindle Centrosome at Daughter envelope
one spindle pole chromosomes forming
Figure 12.7c
10 m
G2 of Interphase Prophase Prometaphase
Figure 12.7d
10 m
Metaphase Anaphase Telophase and Cytokinesis
Figure 12.7e
Figure 12.7f
Figure 12.7g
Figure 12.7h
Figure 12.7i
Figure 12.7j
The Mitotic Spindle: A Closer Look
• The mitotic spindle is a structure made of
microtubules that controls chromosome movement
during mitosis
• In animal cells, assembly of spindle microtubules
begins in the centrosome, the microtubule
organizing center
• The centrosome replicates during interphase,
forming two centrosomes that migrate to opposite
ends of the cell during prophase and
prometaphase
Centrosome
Aster
Metaphase
Sister plate
chromatids (imaginary) Microtubules
Chromosomes
Kineto-
chores Centrosome
1 m
Overlapping
nonkinetochore
microtubules Kinetochore
microtubules
0.5 m
Figure 12.8a
Kinetochores
Kinetochore
microtubules
0.5 m
Figure 12.8b
Microtubules
Chromosomes
Centrosome
1 m
• In anaphase, sister chromatids separate and move
along the kinetochore microtubules toward
opposite ends of the cell
• The microtubules shorten by depolymerizing at
their kinetochore ends
Spindle
pole
Mark
RESULTS
CONCLUSION
Chromosome
movement
Microtubule Kinetochore
Spindle
pole
Mark
RESULTS
Figure 12.9b
CONCLUSION
Chromosome
movement
Microtubule Kinetochore
(a) Cleavage of an animal cell (SEM) (b) Cell plate formation in a plant cell (TEM)
100 m
Cleavage furrow Vesicles Wall of parent cell
forming 1 m
cell plate Cell plate New cell wall
100 m
Cleavage furrow
Daughter cells
Figure 12.10d
Chromatin
Nucleus condensing
10 m
Nucleolus Chromosomes Cell plate
Chromatin
Nucleus condensing
Nucleolus
10 m
1 Prophase
Figure 12.11b
Chromosomes
10 m
2 Prometaphase
Figure 12.11c
10 m
3 Metaphase
Figure 12.11d
10 m
4 Anaphase
Figure 12.11e
10 m
Cell plate
5 Telophase
Binary Fission in Bacteria
• Prokaryotes (bacteria and archaea) reproduce by
a type of cell division called binary fission
• In binary fission, the chromosome replicates
(beginning at the origin of replication), and the
two daughter chromosomes actively move apart
• The plasma membrane pinches inward, dividing
the cell into two
3 Replication
finishes.
Figure 12.12-4
Origin of Cell wall
replication Plasma membrane
E. coli cell
Bacterial chromosome
1 Chromosome Two copies
replication of origin
begins.
3 Replication
finishes.
4 Two daughter
cells result.
The Evolution of Mitosis
• Since prokaryotes evolved before eukaryotes,
mitosis probably evolved from binary fission
• Certain protists exhibit types of cell division that
seem intermediate between binary fission and
mitosis
Chromosomes
Microtubules
(b) Dinoflagellates
Intact nuclear
envelope
Kinetochore
microtubule
(c) Diatoms and
some yeasts Intact nuclear
envelope
Kinetochore
microtubule
(d) Most eukaryotes
Fragments of
nuclear envelope
Figure 12.13a
Bacterial
chromosome
(a) Bacteria
Chromosomes
Microtubules
Intact nuclear
envelope
(b) Dinoflagellates
Figure 12.13b
Kinetochore
microtubule
Intact nuclear
envelope
Kinetochore
microtubule
Fragments of
nuclear envelope
(d) Most eukaryotes
Concept 12.3: The eukaryotic cell cycle is
regulated by a molecular control system
• The frequency of cell division varies with the type
of cell
• These differences result from regulation at the
molecular level
• Cancer cells manage to escape the usual controls
on the cell cycle
S G1 M G1
RESULTS
S S M M
When a cell in the S When a cell in the
phase was fused M phase was fused with
with a cell in G1, a cell in G1, the G1
the G1 nucleus nucleus immediately
immediately entered began mitosis—a spindle
the S phase—DNA formed and chromatin
was synthesized. condensed, even though
the chromosome had not
been duplicated.
The Cell Cycle Control System
• The sequential events of the cell cycle are directed
by a distinct cell cycle control system, which is
similar to a clock
• The cell cycle control system is regulated by both
internal and external controls
• The clock has specific checkpoints where the cell
cycle stops until a go-ahead signal is received
Control
system S
G1
M G2
M checkpoint
G2 checkpoint
• For many cells, the G1 checkpoint seems to be the
most important
• If a cell receives a go-ahead signal at the G1
checkpoint, it will usually complete the S, G2, and
M phases and divide
• If the cell does not receive the go-ahead signal, it
will exit the cycle, switching into a nondividing
state called the G0 phase
G0
G1 checkpoint
G1 G1
Time
(a) Fluctuation of MPF activity and cyclin concentration
during the cell cycle
Cdk
Degraded
cyclin G2 Cdk
checkpoint
Cyclin is
degraded
Cyclin
MPF
M G 1 S G2 M G1 S G2 M G1
MPF activity
Cyclin
concentration
Time
(a) Fluctuation of MPF activity and cyclin concentration
during the cell cycle
Figure 12.17b
Cdk
Degraded
cyclin G2 Cdk
checkpoint
Cyclin is
degraded
MPF Cyclin
10 m
• A clear example of external signals is density-
dependent inhibition, in which crowded cells
stop dividing
• Most animal cells also exhibit anchorage
dependence, in which they must be attached to a
substratum in order to divide
• Cancer cells exhibit neither density-dependent
inhibition nor anchorage dependence
Anchorage dependence
Density-dependent inhibition
Density-dependent inhibition
20 m 20 m
(a) Normal mammalian cells (b) Cancer cells
Figure 12.19a
20 m
Figure 12.19b
20 m
Loss of Cell Cycle Controls in Cancer Cells
• Cancer cells do not respond normally to the body’s
control mechanisms
• Cancer cells may not need growth factors to grow
and divide
– They may make their own growth factor
– They may convey a growth factor’s signal without
the presence of the growth factor
– They may have an abnormal cell cycle control
system
Lymph
vessel
Tumor
Blood
vessel
Glandular Cancer
tissue cell
Metastatic
tumor
1 A tumor grows 2 Cancer 3 Cancer cells spread 4 Cancer cells
from a single cells invade through lymph and may survive
cancer cell. neighboring blood vessels to and establish
tissue. other parts of the a new tumor
body. in another part
of the body.
• Recent advances in understanding the cell
cycle and cell cycle signaling have led to
advances in cancer treatment