Meat Science 62 (2002) 373380

www.elsevier.com/locate/meatsci

Antimicrobial food packaging in meat industry

Stefania Quintavalla*, Loredana Vicini
Experimental Station for the Food Preserving Industry, V.le Tanara, 31/A, 43100 Parma, Italy
Received 20 March 2002; received in revised form 4 May 2002; accepted 14 May 2002

Abstract
Antimicrobial packaging, an active packaging concept, can be considered an extremely challenging technology that could have a
signicant impact on shelf-life extension and food safety of meat and meat products. Use of antimicrobial substances can control
the microbial population and target specic microorganisms to provide higher safety and quality products. Many classes of antimicrobial compounds have been evaluated in lm structures, both synthetic polymers and edible lms: organic acids and their salts,
enzymes, bacteriocins, and miscellaneous compounds such as triclosan, silver zeolites, and fungicides.
The characteristics of some antimicrobial packaging systems are reviewed in this article. The regulatory status of antimicrobial
packaging in EU is also examined. # 2002 Elsevier Science Ltd. All rights reserved.
Keywords: Active packaging; Antimicrobial substances; Meat; Meat products; Pathogens

1. Introduction
Active packaging is one of the innovative food
packaging concepts that have been introduced as a
response to the continuous changes in current consumer
demands and market trends. It has been dened as a
type of packaging that changes the condition of the
packaging to extend shelf-life or improve safety or sensory properties while maintaining the quality of the
food. This denition of active packaging was chosen
for the European FAIR-project CT 98-4170 (Vermeiren, Devlieghere, van Beest, de Kruijf, & Debevere,
1999).
In general, active food packaging can provide several
functions that do not exist in conventional packaging
systems. The active functions may include scavenging of
oxygen, moisture or ethylene, emission of ethanol and
avours, and antimicrobial activity.
Microbial contamination reduces the shelf-life of
foods and increases the risk of foodborne illness. Traditional methods of preserving foods from the eect of
microbial growth include thermal processing, drying,
freezing, refrigeration, irradiation, modied atmosphere
packaging, and adding antimicrobial agents or salts.
* Corresponding author. Tel.: +39-0521-795265; fax: +39-0521771829.
E-mail address: [email protected] (S. Quintavalla).

Unfortunately, some of these techniques cannot be

applied to some food products, such as fresh meats and
ready-to-eat products.
Antimicrobial packaging is a promising form of active
food packaging, in particular for meat products. Since
microbial contamination of these foods occurs primarily
at the surface, due to post-processing handling,
attempts have been made to improve safety and to delay
spoilage by use of antibacterial sprays or dips. However,
direct surface application of antibacterial substances
onto foods have limited benets because the active substances are neutralized on contact or diuse rapidly
from the surface into the food mass. On the other hand,
incorporation of bactericidal or bacteriostatic agents
into meat formulations may result in partial inactivation of the active substances by product constituents
and is therefore expected to have only limited eect on
the surface microora.
Therefore, the use of packaging lms containing antimicrobial agents could be more ecient, by slow
migration of the agents from the packaging material to
the surface of the product, thus helping maintain high
concentrations where they are needed. If an antimicrobial can be released from the package during an
extended period, the activity can also be extended into
the transport and storage phase of food distribution.
Antimicrobial substances incorporated into packaging materials can control microbial contamination by

0309-1740/02/$ - see front matter # 2002 Elsevier Science Ltd. All rights reserved.
PII: S0309-1740(02)00121-3

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S. Quintavalla, L. Vicini / Meat Science 62 (2002) 373380

reducing the growth rate and maximum growth population and/or extending the lag-phase of the target
microorganism, or by inactivating microorganisms by
contact.

2. Developing the antimicrobial packaging systems

Most food packaging systems represent either a
package/food system or a package/headspace/food system (Fig. 1).
A package/food system is a solid food product in
contact with the packaging material, or a low-viscosity
or liquid food without headspace. Individually wrapped
ready-to-eat meat products, sous-vide cooked products and deli products are good examples. Diusion
between the packaging material and the food and partitioning at the interface are the main migration phenomena involved in this system. Antimicrobial agents
may be incorporated into the packaging materials initially and migrate into the food through diusion and
partitioning (Han, 2000).
Package/headspace/food systems are represented by
foods packed in exible packages, cups, and cartons.
Evaporation or equilibrated distribution of a substance
among the headspace, packaging material and/or food
has to be considered as a part of main migration
mechanisms to estimate the interfacial distribution of
the substance. A volatile active substance can be used in

Fig. 1. Food packaging systems and relative behaviour of active substances (Han, 2000).

these systems, as it can migrate through the headspace

and air gaps between the package and the food.
Besides diusion and equilibrated sorption, some
antimicrobial packaging uses covalently immobilized
antibiotics or fungicides, or active moieties such as
amine groups. This case utilizes surface inhibition of
microbial growth by immobilization of the non-foodgrade antimicrobial substance without diusional mass
transfer.
Fig. 2 shows the mass transfer phenomena of an
active substance incorporated into a lm or coating,
with dierent applications.
The incorporation of an antimicrobial substance into
a food packaging system can take several approaches.
One is to put the antimicrobial into the lm by adding it
in the extruder when the lm or the co-extruded lm is
produced. The disadvantage of doing so is poor cost
eectiveness since antimicrobial material not exposed
to the surface of the lm is generally not totally available to antimicrobial activity. An alternative to extrusion
is to apply the antimicrobial additive in a controlled
matter where the material is needed and not lost; for
example, it can be incorporated into the food-contact
layer (usually also serving as the inner heat-seal layer) of
a multilayer packaging material.
According to Han (2000), several factors must be
taken into account in the design or modelling of the
antimicrobial lm or package:
Chemical nature of lms/coatings, casting process conditions and residual antimicrobial activity. The choice
of the antimicrobial is often limited by the heat lability of the component during extrusion or by the
incompatibility of the component with the packaging
material. For example, 1% potassium sorbate in a
LDPE lm inhibited the growth of yeast on agar
plates. The LDPE resin and potassium sorbate powder can be mixed, extruded and pelletized to produce
a master batch. These pellets can be added to LDPE
resin. The master batch should be produced at low
temperature to prevent heat decomposition of the
potassium sorbate (Han & Floros, 1997). Another
study (Weng & Hotchkiss, 1993), however, found the
relatively polar sorbate, benzoate and propionate to
be incompatible with the apolar LDPE. Acid anhydrides were thought to be more compatible than free
acids and their salts because of their lower polarity.
The residual antimicrobial activity is the eective
activity of the antimicrobial agents utilized after the
casting (extrusion) and converting processes (lamination, printing, drying). The eects of adhesives and
solvents should also be characterized quantitatively.
Characteristics of antimicrobial substances and foods.
Food components signicantly aect the eectiveness
of the antimicrobial substances and their release.
Physico-chemical characteristics of food could alter the

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375

Fig. 2. Migration of active substance in dierent applications of antimicrobial packaging systems (Han, 2000).

activity of antimicrobial substances. For example,

the pH of food inuences the ionisation (dissociation/association) of most active chemicals, and could
change the antimicrobial activity of organic acids
and their salts. The antimicrobial activity and chemical stability of incorporated active substances
could be inuenced also by the water activity of
food. Moreover, each food has its own characteristic
microora. The release kinetics of antimicrobial
agents has to be designed to maintain the concentration above the critical inhibitory concentration
with respect to the contaminating microorganisms
that are likely to be present.
Storage temperature. Storage temperature can aect
the antimicrobial activity of chemical preservatives.
Generally, increased storage temperature can accelerate the migration of the active agents in the lm/
coating layers, while refrigeration slows down the
migration rate. The temperature conditions during
production and distribution have to be predicted to
determine their eect on the residual antimicrobial
activity of the active compounds.
Mass transfer coecients. The simplest system is
the diusional release of active substances from the

package into the food. A multilayer design has the

advantage that the antimicrobial can be added in one
thin-layer and its migration and release controlled by
the thickness of the lm layer or coating. In practice,
a matrix of several layers are used to control the rate
of release of the active substance. Control of the
release rates and migration amounts of antimicrobial
substances from food packaging is very important. A
mass transfer model of the migration phenomena can
be used to describe the concentration prole in the
lm/coating layer and food over time. Han (2000)
summarized traditional mass transfer models and his
own proposed models that may be used to describe
the migration of active agents through food packaging systems consisting of single, double, or triple
layers. By using mass transfer models it is possible to
calculate the storage periods that maintain the active
agent concentration above the critical eectiveness
concentration, so safety shelf-life of the food could be
calculated.
Physical properties of packaging materials. When
antimicrobial activity is added to packaging materials
to reduce microbial growth, it may aect the general
physical properties of the packaging materials. Han

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S. Quintavalla, L. Vicini / Meat Science 62 (2002) 373380

and Floros (1997) found that the transparency of the

plastic lm under study decreased with the addition
of the active agent. The performance of the packaging
materials must be maintained after the addition of the
active substances, even though the materials contain
more heterogeneous formulations.

3. Examples of antimicrobial packaging concepts

As previously mentioned, approaches to antimicrobial
packaging can be classied as either of two types. The
rst consists of binding an agent to the surface of the
package and this would require a molecular structure
large enough to retain activity on the microbial cell wall
even though bound to the plastic. Such agents are likely
to be limited to enzymes or other antimicrobial proteins.
The second approach involves the release of active
agents onto the surface of the food.
Non-edible packaging systems may contain any type
of food grade additives in their packaging materials.
Some chemical agents naturally exist in plants or fermented products. However, they mainly are chemically
synthetized and categorized as chemical additives
sometimes with restricted regulations required.
In Japan, silver-substituted zeolite has been developed
as the most common antimicrobial agent incorporated
into plastics. Silver-ions, which inhibit a wide range of
metabolic enzymes, have strong antimicrobial activity
with a broad spectrum. The zeolite, which has some of
its surface atoms replaced by silver, is laminated as a
thin layer (36 mm) in the surface of the food contact
polymer and appears to release silver ions as aqueous

solution from the food enters the exposed cavities of the

porous structure (Ishitani, 1995). Less than 0.001% of
the silver is in the coating itself. On 9 June 2000 the
AgIONTM Silver Ion Technology received the approval
of the Food and Drug Administration for use in all
types of food-contact polymers in USA market (http://
www.cfsan.fda.gov/
dms/opa-fcn.html).
Several other compounds have been proposed and
tested for antimicrobial activity in food packaging
including organic acids such as sorbate, propionate and
benzoate or their respective acid anhydrides (Table 1),
bacteriocins e.g. nisin and pediocin or enzymes such as
lysozyme (Table 2), and fungicides such as benomyl
(Halek & Garg, 1989) and imazalil (Weng & Hotchkiss,
1992).
An interesting commercial development is the recent
marketing of food-contact approved Microban1
(Microban Products Co., USA) wide range of products such as cutting boards and dishcloths, which
contain triclosan (2,4,40 -trichloro-20 -hydroxydiphenylether, also used in soaps, shampoos, and toothbrushes).
Cutter (1999) investigated the eectiveness of triclosanincorporated plastic (TIP) against populations of foodborne pathogenic bacteria as well as bacteria associated
with meat surface. Plate overlay assays indicated that
plastic containing 1500 ppm of triclosan inhibited the
growth of Brochotrix thermosphacta ATCC 11509, Salmonella Typhimurium ATCC 14028, Staphylococcus
aureus ATCC 12598, Bacillus subtilis ATCC 6051, Shigella exneri ATCC 12022, Escherichia coli ATCC 25922
and several strains of Escherichia coli O157:H7. However,
the same lm did not eectively reduce bacterial populations on refrigerated, vacuum-packaged meat surfaces.

Table 1
Applications of antimicrobial food packaging. Incorporation of organic acids and their salts in plastic or edible lms
Antimicrobial agent

Packaging materiala

Substrate

Reference

Acetic acid

Chitosan
Chitosan
PE-co-MA
LDPE
MC/chitosan
WPI
Alginate
Corn zein lm
Corn zein lm
Chitosan
Chitosan
WPI
PE
MC/HPMC/fatty acid
MC/palmitic acid
Starch/glycerol
MC/chitosan
LDPE
LDPE

Water
Bologna, cooked ham, pastrami
Culture media
Culture media
Culture media
Culture media
Lean beef muscle
Culture media
Culture media
Water
Bologna, cooked ham, pastrami
Culture media
Culture media
Culture media
Culture media
Chicken breast
Culture media
Cheese
Culture media

Ouattara et al. (2000a)

Ouattara et al. (2000b)
Weng and Chen (1997)
Weng and Hotchkiss (1993)
Chen, Yeh, and Chiang (1996)
Cagri, Ustunol, and Ryser (2001)
Siragusa and Dickinson (1992)
Padgett, Han, and Dawson (2000)
Homan, Han, and Dawson (2001)
Ouattara et al. (2000a)
Ouattara et al. (2000b)
Cagri et al. (2001)
Weng and Chen (1997)
Vojdani and Torres (1990)
Rico-Pena and Torres (1991)
Baron and Summer (1993)
Chen et al. (1996)
Han (1996)
Han and Floros (1997)

Benzoic acid
Benzoic anhydride
Sodium benzoate
p-aminobenzoic acid
Lactic acid
Lauric acid
Propionic acid
Sorbic acid
Sorbic acid anhydride
Potassium sorbate

LDPE, low-density polyethylene; MC, methyl cellulose; HPMC, hydroxypropyl MC; CMC, carboxyl MC; PE, polyethylene; MA, methacrylic
acid; SPI, soy protein isolate; WPI, whey protein isolate.

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377

Table 2
Applications of antimicrobial food packaging. Incorporation of pediocins and enzymes in plastic or edible lms
Antimicrobial agent

Packaging materiala

Substrate

Reference

Glucose oxidase
Lysozyme

Alginate
PVOH, nylon, cellulose acetate
SPI lm, corn zein lm
Silicon coating
SPI, corn zein lm
PE
Corn zein lm
PVC, LDPE, nylon
PE
HPMC
SPI, WPI, WG, EA
Corn zein lm
Cellulose

Fish
Culture media
Culture media
Beef tissue
Culture media
Broiler drumstick skin
Culture media
Culture media
Phospate buer
Culture media
Phospate buer
Culture media
Cooked meats

Field, Pivarnick, Barnett, and Rand (1986)

Appendini and Hotchkiss (1996)
Padgett et al. (1998)
Daeschel, McGuire, and Al-Makhla (1992)
Padgett et al. (1998)
Siragusa, Cutter, and Willett (1999)
Padgett et al. (2000)
Natrajan and Sheldon (2000)
Cutter, Willett, and Siragusa (2001)
Coma, Sebti, Pardon, Deschamps, and Pichavant (2001)
Ko, Janes, Hettiarachchy, and Johnson (2001)
Homan et al. (2001)
Ming et al. (1997)

Nisin

Pediocin

a
LDPE, low-density polyethylene; MC, methyl cellulose; HPMC, hydroxypropyl MC; PE, polyethylene; PVOH, polyvinyl alcohol; PVC, polyvinyl chloride; SPI, soy protein isolate; WPI, whey protein isolate; WG, wheat gluten; EA, egg albumen.

The presence of fatty acids or other components associated with adipose tissue might diminish the antibacterial activity of TIP on meat surfaces. Recently, the
use of triclosan for food-contact applications has been
allowed in EU countries by the SCF (Scientic Committee for Food) in the 10th additional list of monomers and
additives for food contact materials (SCF, 2000), with a
quantitative restriction on migration of 5 mg/kg of food.
In contrast to conventional antimicrobial lms, some
functional groups that have antimicrobial activity have
been immobilized on the surface of polymer lms by
modied chemical methods to prevent the transfer or
migration of antimicrobial substances from the polymer
to the food (Haynie, Crum, & Doele, 1995).
Modifying surface composition of polymers by electron irradiation in such a way that the surface would
contain amine groups has also been shown to exhibit
antimicrobial activity that inactivates microorganisms
by contact. Cohen et al. (1995) reported that exposure
of nylon yarn or fabric to 13 J/cm2 of 193 nm light
from an excimer laser in air caused an apparent 10%
conversion of amides to amines (resulting in 51012
surface amines/cm2), which are still bound in the polymer chain.
The surface-bound amine groups proved active in
phosphate buer against Staphylococcus aureus ATCC
25923, Pseudomonas uorescens ATCC 13525 and
Enterococcus faecalis ATCC 19433. The decrease in
bacterial concentration was more likely due to bactericidal action rather than surface adsorption of the cells
(Paik, Dhanasekharan, & Kelly, 1998).
Microbial activity on such nylon lms is closely related to the laser wavelength used in the process, because
laser irradiation at 248 nm does not change surface
chemistry or initiate the conversion of amide moieties
on the surface to amine groups.
Shearer, Paik, Hoover, Haynie, and Kelley (2000)
irradiated a nylon 6,6 lm with a 193 nm UV excimer.

The irradiated nylon demonstrated antagonistic activity

against Staphylococcus aureus ATCC 25923 and
Escherichia coli TV1058 with 4.5 and 6.0 log reductions,
respectively, of an initial population of 106 cfu/ml. The
irradiated nylon was ineective against Pseudomonas
uorescens ATCC 13525 and Enterococcus faecalis
ATCC 19433 under similar conditions. However, protein and salt inhibited the antimicrobial eect of the
irradiated nylon lm.
Other applications of the laser-induced surface modications may be used to produce antimicrobial packaging systems in the future. UV irradiation, like that
produced by an UV excimer laser at an appropriate
wavelength, can be used to oxidize O2, previously
absorbed on a modied surface layer, to O3 by photochemical means (Rooney, 1995). The formed O3 can
then be desorbed or subjected to controlled-release from
the polymer matrix to the interior of the package. Even
ppm concentrations of O3 are sucient to inhibit
microbial growth and this approach could signicantly
overcome the necessity of direct contact between the
food and antimicrobial lm to be eective.
Not only UV treatments can be used to modify lm
surfaces. Plasma treatments have gained considerable
attention in the food packaging area as methods to
improve adhesion, sealability, wettability and other
characteristics of polymers (Ozdemir, Yurteri, & Sadikoglu, 1999). For example, uorine-based plasmas can
be used to form a uorinated surface layer on food
packaging polymer surfaces, and the antimicrobial
properties of halogens have been known for years.
3.1. Antimicrobial edible coatings and lms
There is a growing interest in edible coatings due to
factors such as environmental concerns, need for new
storage techniques, and opportunities for creating
new markets for under-utilized agricultural commodities

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S. Quintavalla, L. Vicini / Meat Science 62 (2002) 373380

with lm-forming properties. Edible coatings and lms

prepared from polysaccharides, proteins and lipids have
a variety of advantages such as biodegradability, edibility, biocompatibility, aesthetic appearance and barrier
properties against oxygen and physical stress.
Some advantages of using edible coatings and lms
on meat and meat products have been discussed recently
by Gennadios, Hanna, and Kurth (1997). Edible coatings could:
 help alleviate the problem of moisture loss during storage of fresh or frozen meats;
 hold juices of fresh meat and poultry cuts when
packed in retail plastic trays;
 reduce the rate of rancidity-causing lipid oxidation and brown coloration-causing myoglobin
oxidation;
 reduce the load of spoilage and pathogen microorganisms on the surface of coated meats; and
 restrict volatile avour loss and foreign odour
pick-up.
As an application of active packaging, edible coatings
carrying antioxidants or antimicrobials can be used for
direct treatment of meat surfaces.
In the case of edible lms and coatings, selection of
the incorporated active agents is limited to edible compounds. Because they have to be consumed with edible
lm/coating layers and foods together, their edibility
and safety are essential.
Antimicrobial edible lms have received attention as a
potential pathogen intervention strategy for various
muscle foods. Siragusa and Dickinson (1993) demonstrated that alginate coatings containing organic acids
were marginally eective on beef carcasses, reducing
levels Listeria monocytogenes, Salmonella Typhimurium
and Escherichia coli O157:H7 by 1.80, 2.11, and 0.74
logs, respectively.
Complete inhibition of Listeria monocytogenes on
ham, turkey breast meat and beef was obtained using
pediocin or nisin xed on a cellulose casing (Ming,
Weber, Ayres, & Sandine, 1997). Commercial application of this technology is described in US Patent
5,573,797 assigned to Viskase Co. The package is a lm,
such as a polymer lm or a regenerated cellulose
lm, containing a heat resistant, Pediococcus-derived
bacteriocin in a synergistic combination with a chelating
agent to inhibit or kill Listeria monocytogenes on contact with food (Katz, 1999).
Dawson, Han, and Padgett (1997) and Padgett, Han,
and Dawson (1998) used nisin and lysozyme in soy
protein and corn zein lms to inhibit Lactobacillus
plantarum and Escherichia coli on laboratory media.
Lactobacillus plantarum was inhibited by lm containing
nisin or lysozyme; the addition of EDTA increased the
inhibitory eect of lms against Escherichia coli.

Ouattara, Simard, Piette, Begin, and Holley (2000a,

2000b) prepared antimicrobial lms by inclusion of
various organic acids and essential oils into a chitosan
matrix, and investigated the ability of these lms to
inhibit the growth of indigenous (lactic acid bacteria
and Enterobacteriaceae) or inoculated bacteria (Lactobacillus sakei and Serratia liquefaciens) onto the surfaces
of vacuum-packed cured meat products. Release of
organic acids (acetic and propionic acid) was found to
be initially fast, when the gradient of ion concentration
between the inside of the polymer matrix and the outside environment was high, then decreased as the release
of acids progressed. Whereas the antimicrobial lms
under study did not aect lactic acid bacteria, the
growth of Enterobacteriaceae and Serratia liquefaciens
was delayed or completely inhibited after a storage of 21
days at 4  C. Strongest inhibition was observed on surfaces with lower water activity value (bologna), onto
which acid release was slower, and with lms containing
cinnamaldeyde, as a result of its greater antimicrobial
activity under these conditions.

4. Regulatory status of antimicrobial packaging in EU

The general problem arising from the use of food
contact materials depends on their content of substances capable of migrating into the contacted food.
Therefore, to protect the consumer, an assessment of
the potential hazards from oral exposure to those constituents that migrate into the food must be made.
Gergely (2001) provided a clear vision of the regulatory status regarding the use of antimicrobials in
food applications in EU.
In general, antimicrobial substances fall under the
denition of biocidal products:
active substances (and preparations containing one
or more active substances, put up in the form in
which they are supplied to the user), intended to
destroy, deter, render harmless, prevent the action
of, or otherwise exert a controlling eect of any
harmful organism by chemical or biological means.
provided in Directive 98/8/EC, called Biocide Directive (EU, 1998). This Directive excludes many product
categories, falling under other EU directives; hence, on
the basis of this exclusion, the category lm preservatives, listed in one of the Annexes of the directive,
would only cover those antimicrobial products used for
the preservation of plastic materials in general, but not
antimicrobials used in food contact plastic applications.
Also nished packaging materials containing antimicrobial substances would not be covered by the Biocide Directive, even for general applications, because
the directive limits the denition of biocides to cover

S. Quintavalla, L. Vicini / Meat Science 62 (2002) 373380

substances that are put in the form in which they are

supplied to the user.
As a consequence, antimicrobial additives are permitted in food packaging under the Plastics Directive,
Directive 90/128/EEC as last updated by Directive
2002/17/EC (EU, 2002) and, for substances that are not
covered by a listing in the Plastics Directive, the
national legislation in place in each of the individual
Member Sates must be consulted.
However, the use of biocides is only compliant with
the requirements of these laws if they are required to
manufacture a nished product, without having a direct
impact on the quality of the packaged food.
In practice, the producer presenting an application for
safety assessment of a substance to be used in food
contact materials should provide evidence (1) that any
migration into food is not intentional but only incidental; (2) that its use does not exert any preservative
eect on the food; (3) that it does not allow the selection
of non-sensitive organisms on the surface of the food
contact materials and that it does not allow the development of biocide resistance in sensitive microorganisms. The petitioner should also provide evidence that
the substance is not used to reduce the normal hygienic
measures required in handling foodstus (SCF, 2001).
If the second criterion is not met, the product must be
considered a direct food additive (a preservative that
prolongs the shelf-life of foodstus by protecting them
against deterioration caused by microorganisms) and
would fall under the Miscellaneous Additives Directive
(EU, 1995).
It is quite dicult to draw a strict line where the
technical function of an additive is solely its antimicrobial eect on the packaging without an impact on
the food itself. Most of the materials used in antimicrobial packaging systems only act as a reservoir for
the active substance and the target eect is on the food
in contact with the package. This is clearly in conict
with the Plastics Directive.
The EU-wide regulatory instruments covering antimicrobial substances dier distinctively in scope,
depending upon both the intended application and the
actual eect of the active substances. So far, it is not the
active product itself, but its use conditions that determine which directive applies in regulating the marketing
of food packaging products with antimicrobial activity
in the European Union.

5. Conclusions
Consumers continue to demand foods that are minimally processed and posses fresh-like quality, while
modern distribution systems require an adequate shelflife. Numerous type of food packaging can be used in
combination with food preservation techniques in order

379

to extend the eectiveness of food preservation chain.

The idea of combining antimicrobials with packaging
lms to control the growth of microorganisms in food
could have a signicant impact on shelf-life extension
and food safety.
The application of antimicrobial lms might allow for
migration of the antimicrobial to the lm surface and
therefore a continued antimicrobial eect at the food
surface during extended exposure. Direct addition of
antimicrobials to food will result in an immediate
reduction of bacterial population but this technique
may not account for the recovery of injured cells or the
growth of cells that were not destroyed by direct addition.
The use of antimicrobial packaging materials in food
packaging can minimize the microbial contamination of
food product surfaces during storage, transportation
and handling. The main action of these lms is based on
the release of antimicrobial substances into food products. Some of these agents could pose a safety risk to
consumers if the release is not tightly controlled by
some mechanism within the packaging material itself.
An interesting innovation could be the use of polymers
which surfaces have been modied by electron irradiation or plasma treatment to generate antimicrobial
activity without any transfer or migration of substances
to the food.
As the growth and death rates of bacteria will vary for
each growth medium, conclusions on how antimicrobial
lms will perform with a food product must be determined for each food application.

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