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    Molecular Biotechnology

    Uploaded by

    Bhaskar Ganguly
    The document discusses the fundamentals and applications of molecular biotechnology. It defines molecular biotechnology as dealing with the deliberate use of biomolecules for creating products and services, focusing on principles like recombinant DNA technology. Examples of applications discussed include using genetic engineering to produce insulin in E. coli, creating transgenic goats to express proteins in their milk, and developing gene therapy and gene knockout technologies.

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    Molecular Biotechnology

    Uploaded by

    Bhaskar Ganguly
    148 views40 pages
    The document discusses the fundamentals and applications of molecular biotechnology. It defines molecular biotechnology as dealing with the deliberate use of biomolecules for creating products and services, focusing on principles like recombinant DNA technology. Examples of applications discussed include using genetic engineering to produce insulin in E. coli, creating transgenic goats to express proteins in their milk, and developing gene therapy and gene knockout technologies.

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    Principles and Applications of Molecular Biotechnology

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    The document discusses the fundamentals and applications of molecular biotechnology. It defines molecular biotechnology as dealing with the deliberate use of biomolecules for creating products and services, focusing on principles like recombinant DNA technology. Examples of applications discussed include using genetic engineering to produce insulin in E. coli, creating transgenic goats to express proteins in their milk, and developing gene therapy and gene knockout technologies.

    Copyright:

    © All Rights Reserved
    Download as PDF, TXT or read online from Scribd
    Download as pdf or txt
    148 views40 pages

    Molecular Biotechnology

    Uploaded by

    Bhaskar Ganguly
    The document discusses the fundamentals and applications of molecular biotechnology. It defines molecular biotechnology as dealing with the deliberate use of biomolecules for creating products and services, focusing on principles like recombinant DNA technology. Examples of applications discussed include using genetic engineering to produce insulin in E. coli, creating transgenic goats to express proteins in their milk, and developing gene therapy and gene knockout technologies.

    Copyright:

    © All Rights Reserved
    Download as PDF, TXT or read online from Scribd
    Download as pdf or txt
    of 40

    from principles to applications

    Dr. Bhaskar Ganguly


    Ph.D., M.V.Sc., B.V.Sc. & A.H.

    Molecular Biology
    Probably coined by Sir William Astbury
    Study of biology in molecular terms
    Central Dogma of Molecular Biology:

    Biotechnology
    Coined by Karl Ereky, a Hungarian
    agriculturist, in 1919
    bios = life
    teuchos = tools
    A science that deals with studying, creating
    and using biological tools

    Molecular Biotechnology
    Deals with the deliberate use of biomolecules
    for the creation of services and products
    Until recently, it largely concerned the
    principles and applications of recombinant
    DNA (exploitation of the Central Dogma)
    RNA interference: new paradigm in molecular
    biotechnology

    Fundamentals of Molecular
    Biotechnology:
    The Molecules

    Fundamentals of Molecular Biotechnology

    DNA: biochemical structure

    DNA: biomolecular structure

    Fundamentals of Molecular Biotechnology

    A typical gene structure

    Fundamentals of Molecular Biotechnology

    DNA: Replication

    Fundamentals of Molecular Biotechnology

    The Eukaryotic transcription is much complex!

    DNA: The transcription machinery

    Fundamentals of Molecular Biotechnology

    DNA: Transcription

    Fundamentals of Molecular Biotechnology

    RNA: 5S rRNA and tRNA

    Fundamentals of Molecular Biotechnology

    1: Genetic Code

    2: Translation

    Fundamentals of Molecular Biotechnology

    Expression of Genetic Information

    Fundamentals of Molecular Biotechnology

    Control of expression

    Fundamentals of Molecular
    Biotechnology:
    Recombinant (r) DNA Technology

    Fundamentals of Molecular Biotechnology

    A: Electrophoresis Unit

    2: Agarose Gel Electrophoresis

    Fundamentals of Molecular Biotechnology

    Action of Restriction Enzyme on DNA

    Fundamentals of Molecular Biotechnology

    Restriction Fragment Length Polymorphism

    Fundamentals of Molecular Biotechnology

    Cloning Vectors

    Fundamentals of Molecular Biotechnology

    Cloning Vectors

    Fundamentals of Molecular Biotechnology

    Poly-linker/ Multiple Cloning Site

    Fundamentals of Molecular Biotechnology

    Transformation

    Fundamentals of Molecular Biotechnology

    Selecting positive clones: insertional inactivation

    Fundamentals of Molecular Biotechnology

    Selecting positive clones: alpha complementation

    Fundamentals of Molecular Biotechnology

    Creating a DNA library

    Fundamentals of Molecular Biotechnology

    A typical expression vector

    Fundamentals of Molecular Biotechnology

    Using Tags for protein isolation: 6His Tag

    Fundamentals of Molecular Biotechnology

    PCR based cloning

    Fundamentals of Molecular Biotechnology

    PCR based cloning

    Fundamentals of Molecular Biotechnology

    PCR based cloning

    Antisense RNA Technology

    Applications of Molecular
    Biotechnology

    Applications of Molecular Biotechnology

    Pharming of Insulin in E. coli

    Applications of Molecular Biotechnology

    A typical milk expression construct for goats

    Applications of Molecular Biotechnology

    Comparison of expression systems

    Applications of Molecular Biotechnology

    Gene Therapy

    Applications of Molecular Biotechnology

    Gene Knockout Technology

    Applications of Molecular Biotechnology

    Transgenesis

    Applications of Molecular Biotechnology

    Bioluminescent art

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