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    Biology Practical Reports For Form 4 Experiment 3.6 (Practical Textbook Page 30)

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    1) The document describes a biology experiment to determine the concentration of a sucrose solution isotonic to potato cell sap. Potato strips were immersed for 1 hour in distilled water, 5% sucrose solution, and 30% sucrose solution. 2) The initial and final mass of each strip was measured and the percent mass difference calculated. A graph of percent mass difference versus sucrose concentration was plotted to identify the isotonic point. 3) The experiment found that potato strips immersed in the isotonic 5% sucrose solution experienced no change in mass, supporting the hypothesis that plant cells in an isotonic solution experience no net change in size.

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    Biology Practical Reports For Form 4 Experiment 3.6 (Practical Textbook Page 30)

    Uploaded by

    ke2
    3K views2 pages
    1) The document describes a biology experiment to determine the concentration of a sucrose solution isotonic to potato cell sap. Potato strips were immersed for 1 hour in distilled water, 5% sucrose solution, and 30% sucrose solution. 2) The initial and final mass of each strip was measured and the percent mass difference calculated. A graph of percent mass difference versus sucrose concentration was plotted to identify the isotonic point. 3) The experiment found that potato strips immersed in the isotonic 5% sucrose solution experienced no change in mass, supporting the hypothesis that plant cells in an isotonic solution experience no net change in size.

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    bio practical report

    Original Title

    bio practical report Activity 3.6StudentIsotonic Solution

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    1) The document describes a biology experiment to determine the concentration of a sucrose solution isotonic to potato cell sap. Potato strips were immersed for 1 hour in distilled water, 5% sucrose solution, and 30% sucrose solution. 2) The initial and final mass of each strip was measured and the percent mass difference calculated. A graph of percent mass difference versus sucrose concentration was plotted to identify the isotonic point. 3) The experiment found that potato strips immersed in the isotonic 5% sucrose solution experienced no change in mass, supporting the hypothesis that plant cells in an isotonic solution experience no net change in size.

    Copyright:

    © All Rights Reserved
    Download as DOC, PDF, TXT or read online from Scribd
    Download as doc, pdf, or txt
    3K views2 pages

    Biology Practical Reports For Form 4 Experiment 3.6 (Practical Textbook Page 30)

    Uploaded by

    ke2
    1) The document describes a biology experiment to determine the concentration of a sucrose solution isotonic to potato cell sap. Potato strips were immersed for 1 hour in distilled water, 5% sucrose solution, and 30% sucrose solution. 2) The initial and final mass of each strip was measured and the percent mass difference calculated. A graph of percent mass difference versus sucrose concentration was plotted to identify the isotonic point. 3) The experiment found that potato strips immersed in the isotonic 5% sucrose solution experienced no change in mass, supporting the hypothesis that plant cells in an isotonic solution experience no net change in size.

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    © All Rights Reserved
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    BIOLOGY PRACTICAL REPORTS FOR FORM 4

    Experiment 3.6 (Practical textbook page 30)


    Problem Statement : What is the concentration of an external solution which is isotonic to
    the cell sap of plant tissue?
    Objective : To determine the concentration of an external solution which is isotonic to the cell
    sap of plant tissues.
    Hypothesis :When plant cells are immersed in an isotonic solution, there is no net gain in mass
    and size.
    Variables: manipulated variable: concentration of sucrose solution
    responding variable: mass of strips of potato
    constant variable:
    time of immersion of the potato strips /
    volume of sucrose solution used / type of plant tissue
    used
    Materials: potato strips, distilled water, 5%sucrose solutions, 30% sucrose solution, tissue
    paper
    Apparatus: knife, cork borer, 3 beakers 250ml, electronic weighing scale, ruler
    Technique: record the change in mass of the potato strips that has been immersed for 1 hour in
    different sucrose concentrations using an electronic weighing scale
    Cork Borer

    Potato
    Strip

    Distilled
    Water
    L

    Sucrose
    Solution
    M

    Sucrose
    Solution
    N

    Apparatus set-up to determine the concentration of an external solution which is isotonic to the
    cell sap of plant tissues.
    Procedure:
    1. 3 beakers were labelled as L, M and N. Beaker L was filled with distilled water, beaker M
    with 5% sucrose solution and beaker N with 30% sucrose solution.
    2. A cork borer was used to obtain 3 potato strips. Each strip was cut to a length of 5 cm. The
    potato strips were wiped dry using filter paper. Each potato strip was weighed using an
    electronic weighing scale and the initial mass recorded in a table of results.

    3. The potato strips were placed immersed in the respective sucrose solutions and left for 1 hour.
    After 1 hour, each potato strip was taken out from its respective beaker, wiped dry and its
    mass weighed. The final mass was recorded in a table of results.
    4. The texture was recorded and its appearance noted.
    5. The % difference in mass was calculated.
    6. A graph of % difference in mass against concentration of sucrose solution was drawn and
    from the graph the concentration of sucrose solution which is isotonic to the cell sap of the
    potato was determined.
    7. Safety precaution: The potato strips were wiped dry using tissue paper before weighing to
    get a more accurate mass.
    Results:
    Beaker Concentration
    of sucrose
    solution
    L
    Distilled
    Water
    M
    5%
    N
    30%

    Final
    Initial
    Mass/g Mass/g

    % difference in mass =
    Final mass Initial mass x 100%
    Initial mass

    Texture and
    appearance

    Discussion:
    1. What is the texture and appearance of the potato strips after being immersed in the various
    concentrations of sucrose solution?
    2. What do the axes on your graph represent?
    3. How do you determine the concentration of sucrose solution which is isotonic to the cell sap
    of the potato from your graph?
    4. Based on the experiment, discuss the process of osmosis in the various concentrations of
    sucrose solutions.
    Conclusion:
    The hypothesis is accepted. When plant cells are immersed in an isotonic solution, there is no net
    gain in mass and size.

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