Glukosa Dialab

Liquid Reagent ready to use
GLUCOSE
GOD - PAP
Single Reagent
REAGENT COMPOSITION
STANDARD
COMPONENTS
Phosphate Buffer, pH 7.5
Phenol
4-Aminoantipyrine
Glucose Oxidase
Peroxidase
(has to be ordered separately)

Concentration:
100 mg/dl
Storage:
2 25C
Stability:
up to the expiration date
CLOSE IMMEDIATELY AFTER USE!
CONCENTRATIONS
250 mmol/L
5 mmol/L
0.5 mmol/L
> 10 KU/L
> 1 KU/L
INTERFERING SUBSTANCES
Diagnostic reagent for quantitative in vitro

determination of glucose in human serum or plasma
on photometric systems
REAGENT PREPARATION
no interference up to:
The reagent is ready to use.
ascorbic acid
bilirubin
hemoglobin
triglycerides
REAGENT STABILITY AND STORAGE

Conditions:
D95218B
1 x 1000 ml Single Reagent
D08220
D00221
Storage:
Stability:
protect from light

close immediately after use
at 2 - 8C
up to the expiration date
do not freeze the reagent!
Note: The measurement is not influenced by occasionally

occurring color changes, as long as the absorbance of the
reagent is < 0.3 at 546 nm.
Additionally offered:
D95223
D98485
D98481
D98482
1 x 3 ml
5 x 3 ml
12 x 5 ml
12 x 5 ml
Glucose Standard
Calibrator
Control normal
Control abnormal
Diacal Auto
Diacon N
Diacon P
SAMPLE STABILITY AND STORAGE

Separate at the latest 1h after blood collection from
cellular contents.
15 mg/dl
40 mg/dl
200 mg/dl
2000 mg/dl
MANUAL TEST PROCEDURE

Bring reagents and samples to room temperature.
Pipette into test tubes
Blank
Std/Cal Sample
Reagent
1000 l 1000 l 1000 l
Sample
10 l
Std./Cal.
10 l
Mix. Incubate 10 min. at 37 C or 20 min. at 20 25C.
Measure absorbance of sample and std/cal within
60 minutes against the reagent blank.
Stability after addition of a glycolytic inhibitor (Fluoride,

monoiudacetate, mannose) [4]:
TEST PARAMETERS
Method:
Colorimetric, Endpoint, Increasing

Reaction, GOD - PAP.
Wavelength
500 nm, Hg 546 nm
Temperature:
20-25C or 37C
Sample:
Serum, heparinized or EDTA-plasma,
Linearity:
up to 490 mg/dl (on Hitachi 911)
Sensitivity:
The lower limit of detection is 1 mg/dl.
S:\pm\allg\inserts\inserts_word\clinical chemistry\glucose_god_rev04.doc
Stability:
at 20 25 C 2 days
at 4 8 C 7 days
Stabilityin serum (separated from cellular contents,

hemolysis free) without adding a glycolytic inhibitor [2,5]:
Stability:
at 25 C
at 4 C
8 hours
72 hours
CALCULATION (light path 1 cm)

Glucose (mg/dl) =
A Sample
A Std/Cal
x Conc. of Std/Cal (mg/dl)
UNIT CONVERSION
mg/dl x 0.05551 = mmol/L
Discard contaminated specimens.
Page 1 of 2
DI M. Wagner
Rev. 04, 2009, 01. 16.
REFERENCE RANGE * [1] (mg/dl)

Newborns:
Cord blood
1h
2h
5 14 h
10 28 h
44 52 h
63 - 158
36 - 99
36 89
34 77
46 81
48 79
WASTE MANAGEMENT
A comparison between Dialab Glucose (y) and a

commercially available test (x) using 78 samples gave
following results: y = 1.00 x + 1.00 mg/dl; r= 0.996.
Please refer to local legal requirements.
REFERENCES
QUALITY CONTROL
1.
All control sera with Glucose values determined by this

method can be used.
We recommend:
Children (fasting):
1 6 years
7 19 years
METHOD COMPARISON
74 127
70 106
3.
Adults (fasting):
serum / plasma
2.
70 115
* It is recommended that each laboratory establishes its own normal range.
TEST PRINCIPLE
In the presence of glucose oxidase, glucose is oxidized to
gluconic acid and hydrogen peroxide.
Hydrogen peroxide reacts, in the presence of peroxidase,
with phenol and 4-aminoantipyrine to form a quinoneimine
dye (Trinders reaction) [3].
The intensity of the pink colour formed is proportional to
the glucose concentration.
PERFORMANCE CHARACTERISTICS
LINEARITY
The assay is linear up to 490 mg/dl (on Hitachi 911).
Above this concentration, dilute the sample with NaCl (9
g/L sodium chloride in water) and reassay multiplying the
result by the dilution factor.
PRECISION (at 37C)
Intra-assay
n = 20
Sample 1
Sample 2
Sample 3
Mean
[mg/dl]
64.2
122
296
SD
[mg/dl]
1.12
1.57
4.41
CV
[%]
1.74
4.41
1.49
Inter-assay
n = 20
Sample 1
Sample 2
Sample 3
Mean
[mg/dl]
92.5
121
292
SD
[mg/dl]
1.10
1.02
2.01
CV
[%]
1.19
2.01
0.69
S:\pm\allg\inserts\inserts_word\clinical chemistry\glucose_god_rev04.doc
D98481
12 x 5 ml
DIACON N
Assayed Control
Serum Normal
D98482
12 x 5 ml
DIACON P
Assayed Control
Serum Abnormal
4.
5.
st
Thomas L. Clinical Laboratory Diagnostics. 1 ed. Frankfurt:

TH-Books Verlagsgesellschaft; 1998. p. 131-7.
Sacks DB. Carbohydrates. In: Burtis CA, Ashwood ER,
rd
editors. Tietz Textbook of Clinical Chemistry. 3 ed.
Philadelphia: W.B Saunders Company; 1999. p. 750-808.
Barham D, Trinder P. An improved color reagent for the
determination of blood glucose by the oxidase system.
Analyst 1972;97:142-5.
Guder WG, Zawta B et al. The quality of Diagnostic
st
Samples. 1 ed. Darmstadt: GIT verlag; 2001;p.30-1.
Snacks DB, Bruns DE, Goldstein DE, Mac Laren NK, Mc
Donald JM, Parrott M. Guidelines and recommendations for
laboratory analysis in the diagnosis and management of
diabetes mellitus. Clin Chemi 2002; 48:436-72.
CALIBRATION
The assay requires the use of a Glucose Standard or
Calibrator.
We recommend:
D95223
1 x 3 ml
GLUCOSE STANDARD
D98485
5 x 3 ml
DIACAL
AUTO
Assayed Multi
Calibration Serum
AUTOMATION
Special adaptations for automated analyzers can be made
on request.
WARNINGS AND PRECAUTIONS

1.
2.
The reagent contains sodium azide (0.95 g/L) as

preservative. Do not swallow! Avoid contact with skin
and mucous membranes.
Take the necessary precautions for the use of
laboratory reagents.
Page 2 of 2
DIALAB Produktion und Vertrieb von chemisch technischen

Produkten und Laborinstrumenten Gesellschaft m.b.H.
A 2351 Wiener Neudorf, Austria
IZ-N Sd, Hondastrasse, Objekt M55
Phone: ++43 (0) 2236 660910-0
Fax: ++43 (0) 2236 660910-30 e-mail: [email protected]
DI M. Wagner
Rev. 04, 2009, 01. 16.

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Glukosa Dialab

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Liquid Reagent ready to use

(has to be ordered separately)

Diagnostic reagent for quantitative in vitro

The reagent is ready to use.

REAGENT STABILITY AND STORAGE

1 x 1000 ml Single Reagent

4 x 250 ml Single Reagent

5 x 100 ml Single Reagent

protect from light

Note: The measurement is not influenced by occasionally

SAMPLE STABILITY AND STORAGE

MANUAL TEST PROCEDURE

Stability after addition of a glycolytic inhibitor (Fluoride,

Colorimetric, Endpoint, Increasing

500 nm, Hg 546 nm

Serum, heparinized or EDTA-plasma,

up to 490 mg/dl (on Hitachi 911)

The lower limit of detection is 1 mg/dl.

Stabilityin serum (separated from cellular contents,

CALCULATION (light path 1 cm)

x Conc. of Std/Cal (mg/dl)

Discard contaminated specimens.

Rev. 04, 2009, 01. 16.

REFERENCE RANGE * [1] (mg/dl)

A comparison between Dialab Glucose (y) and a

Please refer to local legal requirements.

All control sera with Glucose values determined by this

* It is recommended that each laboratory establishes its own normal range.

Thomas L. Clinical Laboratory Diagnostics. 1 ed. Frankfurt:

WARNINGS AND PRECAUTIONS

The reagent contains sodium azide (0.95 g/L) as

DIALAB Produktion und Vertrieb von chemisch technischen

Rev. 04, 2009, 01. 16.

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