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    Biuret Test: Objectives

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    chappi0013
    The Biuret test is used to detect the presence of proteins and peptide bonds. Albumin, casein, and gelatin solutions were reacted with sodium hydroxide and copper sulfate, resulting in magenta, eggplant purple, and royal violet colors respectively, indicating the presence of proteins. The test works because copper forms colored chelate complexes with peptide bonds, and the intensity of color corresponds to the number of peptide bonds and protein concentration.

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    Biuret Test: Objectives

    Uploaded by

    chappi0013
    581 views3 pages
    The Biuret test is used to detect the presence of proteins and peptide bonds. Albumin, casein, and gelatin solutions were reacted with sodium hydroxide and copper sulfate, resulting in magenta, eggplant purple, and royal violet colors respectively, indicating the presence of proteins. The test works because copper forms colored chelate complexes with peptide bonds, and the intensity of color corresponds to the number of peptide bonds and protein concentration.

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    Protein detection test Biuret test

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    Biuret Test

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    The Biuret test is used to detect the presence of proteins and peptide bonds. Albumin, casein, and gelatin solutions were reacted with sodium hydroxide and copper sulfate, resulting in magenta, eggplant purple, and royal violet colors respectively, indicating the presence of proteins. The test works because copper forms colored chelate complexes with peptide bonds, and the intensity of color corresponds to the number of peptide bonds and protein concentration.

    Copyright:

    © All Rights Reserved
    Download as DOCX, PDF, TXT or read online from Scribd
    Download as docx, pdf, or txt
    581 views3 pages

    Biuret Test: Objectives

    Uploaded by

    chappi0013
    The Biuret test is used to detect the presence of proteins and peptide bonds. Albumin, casein, and gelatin solutions were reacted with sodium hydroxide and copper sulfate, resulting in magenta, eggplant purple, and royal violet colors respectively, indicating the presence of proteins. The test works because copper forms colored chelate complexes with peptide bonds, and the intensity of color corresponds to the number of peptide bonds and protein concentration.

    Copyright:

    © All Rights Reserved
    Download as DOCX, PDF, TXT or read online from Scribd
    Download as docx, pdf, or txt
    of 3

    Biuret test

    Objectives:
    To determine the presence of proteins and peptide bonds in the solution.
    Materials and Methods:
    2mL each of Albumin, Casein and Gelatin were placed in clean labeled test tubes. An equal volume of 10%
    NaOH solution was then added to each test tube and the solutions were thoroughly mixed. To the resulting
    mixture, 0.5% CuSO
    4
    was added drop wise until a pinkish or violet color was visible.
    Results:
    Solution Results
    Albumin Magenta-colored solution
    Casein Eggplant purple solution
    Gelatin Royal Violet solution



    Discussion:
    The Biuret test is dependent on the ability of Cu (II) to form violet-colored chelate complexes with peptide bonds. It could be
    considered as a qualitative and a quantitative test since the amount violet-colored chelate can be measured using a
    spectrophotometer. Measurement and comparison is made possible by the fact that the intensity of the color produced is
    dependent on the number of peptide bonds reacting, the more protein in the solution the greater the intensity of its color. This is in
    accordance with the Beer-Lambert law which states that absorbance of the sample is directly proportional to the concentration of
    the species.
    The Biuret reagent is a mixture of: Hydrated Copper sulphate which provides the Cu (II) ions which form the chelate complex.
    The Cu (II) ions also gives the reagent its characteristic blue color, Potassium hydroxide solution which does not participate in the
    reaction but provides the alkaline medium needed and Potassium sodium tartrate which works to stabilize the chelate complex.
    Note: This test yields negative for single amino acids and dipeptides because the formation of a chelate complex requires at least
    two peptide bonds.





    Conclusion:
    The Biuret test is a test used for the detection of proteins through reactions with peptide bonds. In the
    experiment all three solutions were positive for peptides but to varying degrees.
    Reference:
    Biuret Test for Protein. (2013). Retrieved August 21, 2014, from http://brilliantbiologystudent.weebly.com/biuret-test-for-
    protein.html

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