2006 by the Society for the Study of Reproduction, Inc.

INDUCTION, DEDUCTION, AND THE SCIENTIFIC METHOD

AN ECLECTIC OVERVIEW OF THE PRACTICE OF SCIENCE
IRVING ROTHCHILD
Emeritus Professor of Reproductive Biology
Case Western Reserve University School of Medicine
Cleveland, Ohio


2006 by the Society for the Study of Reproduction, Inc.

CONTENTS



ABSTRACT 1

INTRODUCTION 1

INDUCTION AND DEDUCTION 2
Etymology 2
Definitions 2
Induction 2
Deduction 3

THE SCIENTIFIC METHOD 3

BEING A SCIENTIST 4
Making Observations 4
Point of View 5
Asking the Right Question 6
Theorizing 6
The theory (or finding) that questions authority 7
Defending the controversial theory or finding 8
Eurekas 8
Experimentation 9
The failed experiment 9
Publishing 10
Statistics 10
Recognition 10

ACKNOWLEDGEMENTS 10

REFERENCES 11

2006 by the Society for the Study of Reproduction, Inc.

1
INDUCTION, DEDUCTION, AND THE SCIENTIFIC METHOD
AN ECLECTIC OVERVIEW OF THE PRACTICE OF SCIENCE
IRVING ROTHCHILD*
Emeritus Professor of Reproductive Biology
Case Western Reserve University School of Medicine
Cleveland, Ohio

ABSTRACT: Science is a never-ending, always changing process through which we learn to
know the material nature of the universe. Science does not deal with nonmaterial entities such as
gods, for there is no way their existence can be either proved or disproved. No single, identifiable
method applies to all branches of science; the only method, in fact, is whatever the scientist can
use to find the solution to a problem. This includes induction, a form of logic that identifies
similarities within a group of particulars, and deduction, a form of logic that identifies a particular
by its resemblance to a set of accepted facts. Both forms of logic are aids to but not the solution
of the scientists problem.
Being a good scientist requires patience, perseverance, imagination, curiosity, and skepticism;
the essence of science is to doubt without adequate proof. Science also requires knowing how to
make and interpret observations (which presupposes a broad point of view), how to ask the right
questions, how to theorize without getting lost in the details, and knowing when to do
experiments and apply statistical tests. Recognition of ones work is desirable but should not be
the primary goal, and publishing papers should be used primarily as a test of the scientists ability
to pursue good science.

*Correspondence: [email protected]


INTRODUCTION
In an essay entitled Is the Scientific Paper a Fraud?
[1], Peter Medewar claimed that induction, in contrast
to deduction, had no place in science. His implication
of fraud was aimed, not at the papers contents, but at
how they were presented, and here he strongly implied
that this presentation was an inductive process. Mede-
war was a great admirer of Karl Popper, a philosopher
of science. In The Logic of Scientific Discovery [2],
Popper rejected induction as a legitimate form of logic
in the practice of science. To bolster his argument a-
gainst induction in science, Medewar cited an unsuc-
cessful attempt by John Stuart Mill to solve problems
in sociology by induction, but neglected to mention
Francis Bacons contribution to the birth of modern
science in the 17th century by the use of induction as a
powerful alternative to Aristotelian and scholastic
dogma.
Popper and Medewar argued vehemently for a
method of scientific practice based on the so-called
hypothetico-deductive system, the essence of which is
the formulation of a hypothesis derived from a collec-
tion of facts, testing the hypothesis by trying to falsi-
fy it, collecting more facts if falsification fails, and
repeating the falsification tests until either you and the
hypothesis agree on a draw or one of you admits de-
feat. Medewar (19151987) shared the 1960 Nobel
Prize in Medicine or Physiology with Sir Frank Mac-
Farlane for their work on the mechanism of tolerance
to acquired immunity. Karl Popper (19021994) was
knighted by Queen Elizabeth II in 1965 and elected a
Fellow of the Royal Society in 1976, so theres no
question here about the kinds of minds were dealing
with.
It is perhaps not too hard to understand that a phil-
osopher, even of science, could make judgments about
any aspect and especially the methods of science, but
what confuses me and Im sure would confuse any
graduate student or postdoc or, in fact, anyone with an
inquiring mind is why someone like Medewar, a prac-
ticing scientist, and certainly no dummy, should get
worked up enough about induction to write an essay
excommunicating it from the scientific community. Is
it really that wicked? Or useless? Should I, as a grad-
uate student, watch my step to make sure I dont ever
use induction in my research? Can I still become a sci-
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2006 by the Society for the Study of Reproduction, Inc.
entist if I do? Should I be careful to use only deductive
reasoning and not lift a finger to make my next ad-
vance into knowledge without first having formulated a
hypothesis? What if I just want to ask a question?
Medewars essay and Poppers philosophy of sci-
ence are a good example of an idiosyncratic viewpoint
about what science is and how it should be practiced. It
is not my own point of view. The purpose of this essay
is to make three main points that emerge from this dif-
ference. The first is that induction is an integral part of
the practice of science and Propper and Medewar,
therefore, in spite of their membership in the class of
intellectual giants, are not only talking nonsense about
induction having no place in science, but are com-
mitting a logical heresy by doing so. The next is that
scientific methods such as hypothetico-deductive [1],
Kochs postulates [3], or any other system based on
rules of procedure or analysis, while they may be legit-
imate ways to practice science, are far from the only
ways to do so. The final point is that certain features of
the practice of science, theorizing, for example, are
essential parts of all branches of science and far more
important than searching for a non-existent only true
scientific method. Most of what I have to say should
be seen only as a perspective of my own ideas about
how we practice science, arising from my familiarity
with the practical and theoretical methods of science,
or having read or heard about or observed being used
by other scientists. Most (if not all) of this has been
said before but that doesnt matter. Each viewpoint,
like each human being, is different and the differences
can sometimes be more interesting than the similar-
ities. A close friend and colleague, for example, disa-
grees with my definition of science! Thats the point.
There is no consensus, even among scientists, about
exactly what science is and every viewpoint, therefore,
can be at least potentially, valuable. It hardly needs
saying that the views expressed in this essay are not
necessarily those of the SSR or its Web site.
Before we get into the nitty-gritty of this essay,
however, a small light touch may help to set the stage,
especially since it serves very well as a pleasant ex-
ample of what science can be all about. In a charming
essay entitled Can an Ape Tell a Joke? [4], Vickie
Hearn describes a problem-solving study in which a
chimpanzee and an orangutan, housed separately, were
each given a small hexagonal block of wood and an
assortment of differently shaped openings into only
one of which the block would fit. They knew they
would be rewarded for making the right choice.
The chimp examined every detail of the floor,
walls, and ceiling; the openings and every side of the
hexagonal block; smelled it, tasted it, and, after trying
one opening after another, found one the block would
fall into. The orangutan scratched his back with the
block, and then sat with a far away look in his eyes for
what seemed to the human observer like forever. He
then put the block directly into the hexagonal opening.
Was the chimp an inductivist? Did the orangutan
consider the problem, form a hypothesis, then test it?
Which one was the scientist? Lets reserve the answer
for the section below called THE SCIENTIFIC
METHOD.
INDUCTION AND DEDUCTION
A commonly held idea of the distinction between
these logical paths to knowledge is that induction is the
formation of a generalization derived from examina-
tion of a set of particulars, while deduction is the iden-
tification of an unknown particular, drawn from its re-
semblance to a set of known facts. For example, if we
examine enough feral cats we can generalize that feral
cats are a rich sources of fleas (induction). If, like Rob-
inson Crusoe, we come across footprints on the beach
of a desert island, we can conclude from our know-
ledge of the human footprint that another human is or
was on the island (deduction).
In fact, however, both terms can have more subtle
meanings. Lets start with a look at their etymology
and definitions.
Etymology
The etymology of these words does not seem to
have any of the judgmental qualities attributed to them
by Popper and Medewar. They come from the Latin
verb ducere, to draw on or along, to pull or drag, to
draw to oneself, to lead, and with the Latin propensity
for prefixes. suffixes, and the modification of the verb
itself, ducere has spawned an enormous population of
derivatives [5]. Even with only the prefixes in and de,
meaning in and from, respectively, both words may
have many more than one meaning. Simply put, to
induce could mean to lead or draw into, to infer, to
persuade, and induction, to lead to the conclusion that
etc.... To deduce could mean to lead from, to draw
from and deduction, to draw a conclusion from etc....
The official lexicographic and practical definitions are
not always much more distinctive.
Definitions
Induction. From The Oxford English Dictionary
(OED); to induce (in relation to science and logic)
means to derive by reasoning, to lead to something as
a conclusion, or inference, to suggest or imply, and
induction as the process of inferring a general law or
principle from observation of particular instances.
Another version is the adducing (pulling together) of
INDUCTION, DEDUCTION, AND THE SCIENTIFIC METHOD 3



2006 by the Society for the Study of Reproduction, Inc.
a number of separate facts, particulars, etc. especially
for the purpose of proving a general statement.
My 1967 edition of the Encyclopedia Britannica (E.
Brit.) gives two versions by John Stuart Mill: the
operation of discovering and proving general proposi-
tions or that operation of the mind by which we infer
that what we know to be true in a particular case or
cases will be true in all cases that resemble the former
in certain assignable respects.
A paraphrase of Francis Bacons view (also from
the E. Brit.) is a selective process of elimination
among a number of alternative possibilities.
The E. Brit. in a separate entry defines primary
induction as the deliberate attempt to find more laws
about the behavior of the thing that we can observe and
so to draw the boundaries of natural possibility more
narrowly (that is, to look for a generalization about
what we can observe), and secondary induction as the
attempt to incorporate the results of primary induction
in an explanatory theory covering a large field of
enquiry (that is, to try to fit the generalization made
by primary induction into a more comprehensive
theory).
E. Mayr in his Growth of Biologic Thought [6]
offers this definition: inductivism claims that (we) can
arrive at objective unbiased conclusions only by
recording, measuring, and describing what we encoun-
ter without any root hypothesis.
Deduction. Sherlock Holmes Elementary, my
dear Watson! has made deduction common know-
ledge a more familiar feature than induction in problem
solving. The OED definition of to deduce is to show
or hold a thing to be derived from etc... or to draw as
a conclusion from something known or assumed, to
infer; deduction thus is inference by reasoning from
generals to particulars, or the process of deducing
from something known or assumed...
Both terms define systems of logic the purpose of
which is to solve problems, in the one case by looking
for a general characteristic (generalization, conclusion,
conjecture, supposition, inference, etc.) in a set or
group of observations, in the other to identify a particu-
lar instance through its resemblance to a set or group of
known instances or observations. Poppers ridicule of
induction was based on the premise that induction re-
quires the observation of every instance of a given
phenomenon for the generalization to be truean
obvious impossibility; the fact that all known crows are
black, for example, doesnt prove that no white crows
exist. Of course it is ridiculous when looked at in this
way, but what really matters is that most if not all
crows are black, and even if a white one should show
up and prove to be a crow and not another kind of bird,
most crows would still be black. His argument can also
be used to make deduction useless for it, too, is based
on an incomplete set of known facts. Even if the
identified fact resembles the members of the set, how
can we be sure that every possible feature of either the
unknown or the members of the set itself has been
considered? As we will see in what follows, in many of
the examples of the way science is practiced, induction
is as much a part of this practice as is deduction or any
system of logic that serves the purpose of advancing
knowledge. Induction and deduction are two, usually
different but never contradictory, approaches to prob-
lem solving. The problem must be solved by testing the
validity of the conclusion or inference, etc. reached
from either direction. Induction and deduction are thus
valuable, often complementary, tools that facilitate
problem solving.
THE SCIENTIFIC METHOD
In spite of what I have said so far, is there a
particular method we can call THE scientific method?
To answer this question it is essential that we first ask
another question: what do we mean by science? The
word comes from Latin scire, to know, and scire
comes from an earlier Latin root meaning to cut
through, i.e. to take apart, to analyze. But science is
more than just knowing by analysis. Science is a
process of learning to know the nature of everything in
the material world, from atoms to the most complex of
living organisms and inanimate objects. Nonmaterial
things, like gods, whose existence can be neither
confirmed nor disproved, are excluded, for science
deals only with those elements of the universe that can
be shown, at least potentially, to exist. Science,
therefore, is never-ending and always changing.
Although its goal is knowledge, it is more than and
different from knowledge itself, for knowledge is its
product not its essence. Its essence is to doubt without
adequate proof. Science is the offspring of philosophy,
and differs from it mainly in the methods used in
learning to know.
As with almost all systems of classification, we
cant draw a sharp distinction between science, as
defined here, and other forms of scholarship as sources
of knowledge such as the OED, Groves Dictionary of
Music and Musicians, the Dickson Baseball Diction-
ary, etc. or even history, for example. In many
respects, history is a science but it is poorly endowed
with or even lacks the ability to predict, one of the
important things that separates science from other
forms of learning.
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2006 by the Society for the Study of Reproduction, Inc.
In all respects science is logically incompatible with
the belief in a nonmaterial intelligent entity that con-
trols the universe and is called God [7, 8], yet many
scientists, especially among the chemists and physicists
but even among some biologists have such a religious
belief. I can think of only three resolutions of this
paradox. The scientists God either is not an intelligent
entity or has no control over the universe. The second
is to accept the concept of science as defined here with
a part of ones mind and that of God with another, with
an impermeable barrier between the two parts. The
third is either not to be a scientist or not to believe in
God, i.e. to be an atheist, or euphemistically, a non-
believer since among many people atheist is a dirty
word. The funny thing about these solutions is that
they all work! The troublemakers are the zealots, i.e.
the proponents of Intelligent Design on the one hand,
and the Russian communists idiotic attempt to prohibit
religion on the other.
A firm distinction between the so-called hard and
soft sciences, e.g. physics and sociology, can also not
be made simply because it is easier to test reality in
some more than in other sciences. Science itself,
therefore, answers our question with a simple but firm
No. There cannot be one method that every kind of
scientific studyin the field, the library, or the lab-
oratorymust follow; the things scientists are curious
about differ too much from one another for all of them
to be studied according to the same or any set of rules
or algorithms.
Medewars caricature of the scientific paper boils
down to a matter of beating a dead horse. He labels it
inductivist because the authors often present their re-
sults without comment and reserve interpretation of
them for the Discussion. In the first place, this isnt
induction. Even Bacon, its chief proponent, saw induc-
tion mainly as a way to separate particulars from one
another into groups of similarities. This is exactly what
the taxonomist does. But even if it were induction (the
meaning of which seems to depend on who defines it),
whats unscientific about saying, Heres what we
found. How would you interpret them? Then well tell
you what we think? Shouldnt a scientific paper be at
least as much fun to read as a good detective story?
There are plenty of things wrong with the way many
scientific papers are written (freight-train adjectives,
misplaced clauses, redundancies, mistakes in grammar
and/or syntax, teleologisms, etc.), but presenting the
results without comment is not one of them. I have a
hunch that Medewar was not a lover of who-done-its!
The only true scientific method is to use whatever
tools we can to make observations, ask and answer
questions, solve problems, test a theory, etc., and it
doesnt matter whether we use induction, deduction, or
any other kind of reasoning to do so; it would be a
heresy to deny the validity of any method that helps us
learn to know. Induction, in fact, resembles what prose
was to Molierres bourgeois gentleman [9]. We use
some form of induction in almost every kind of
scientific endeavor: no matter how it is defined, induc-
tion amounts to making and collating observations.
This was Francis Bacons great contribution to science,
i.e. induction as a path to knowledge through direct
observation of nature [10].
Lets come back now to the chimp and the oran-
gutan. Were both scientists? Yes. Was the orangutan
more so than the chimp? No. He was only different.
Who can say which was better? Are Mayrs contribu-
tions to evolution through ornithology [11] less valu-
able than Dobzhanskys through genetics [12]? Was
Vesalius less a scientist than Mendel because he des-
cribed human anatomy while Mendel did experiments?
Both made observations, one by dissection and the
other by making hybrids. Both increased our know-
ledge of the natural world. Yes, some are better than
others; its how the game is played. We cant all play
the violin like Heifetz, and the likes of a Copernicus, a
Newton, a Darwin, and an Einstein dont make the
headlines every day. But we can all be scientists.
BEING A SCIENTIST
Just as it takes more than talent alone to become a
professional musician, it takes patience, perseverance,
curiosity, imagination, and skepticism to become a
good scientist. These qualities come more as gifts that
can be refined by practice rather than ones that can be
learned. Certain features of the practice of science,
however, can and should be learned for they apply to
every kind of science with equal force. Well look at
them one by one, even though in the real world they
are inseparable and can be fully grasped only as parts
of a whole system.
Making Observations
Observations are the meat and potatoes of science.
We start a research project with observations made
either in the field, the library, or the laboratory. How
these observations are collected, classified, interpreted,
and used as the basis of theorizing (from a hunch to a
eureka) is, more or less, what science is about.
Regardless of our objective, having an open, unbiased
mind with no preconceptions about what we are
looking for is a distinct advantage as we start our
search. Medewar derided this mindset as part of his
indictment of induction, but his prejudice made him
miss its significance. The trick is to have this mindset
INDUCTION, DEDUCTION, AND THE SCIENTIFIC METHOD 5



2006 by the Society for the Study of Reproduction, Inc.
simultaneously with one oriented toward the specific
goal of our investigation, so that, while searching for
information to satisfy our goal, we are also on the alert
for the unexpected, our antennae always ready for the
odd-ball fact that may even change the course of our
investigation. But even if we start making observations
with no specific goal except to learn more about a
given subject, a glimmer of meaning in the form of a
generalization, sometimes even a eureka, will emerge
from the mass of facts and we now look back on all of
them from a different point of view.
For example, Steno, a 17th century anatomist [10],
while dissecting the head of a Great White shark, was
struck by the resemblance of the sharks front teeth to a
common Maltese fossil that the local people called
tongues of stone. Steno eventually identified them as
fossils of shark teeth [13]. This observation, in turn, led
later to the recognition of similar evidence hidden in
sedimentary rocks [13], and this eventually, through a
long series of related discoveries, to Darwins Origin
of Species [14].
Another example, which also brings out the im-
portance of our point of view and of asking the right
question (see below), comes from the science of pale-
ontology. Like the other sciences that rely heavily on
description (e.g. archeology, sociology, linguistics,
even astronomy), the principal source of paleontologic
information comes from the collection of fossils. This
evidence, however, is full of gaps in the record of how
species, genera, families, and so on evolved. Eldredge
and Gould, by seeing these gaps as positive evidence
of how things changed rather than as ignorance about
how they changed, formulated the theory of punctuated
equilibrium [15]. Its essence is that a significant
element in the evolution of life forms consists of long
periods of stasis in form and function punctuated by
relatively short but very active periods of change, too
rapid for fossils to be deposited. Not all biologists
agree with this theory, but many do. It is typical of the
way gathering information by observations alone
(induction) can help the way we learn to know things.
Point of View
Human history is loaded with instances of how a
particular point of view influences or, in fact, deter-
mined it. In science, the importance of our viewpoint
when examining information of any kind cannot be too
strongly emphasized, for how we look at a thing deter-
mines what we see.
The most forceful example, for it so clearly violated
common sense, was Copernicus theory of helio-
centrism. He did not see anything that had escaped the
notice of millions of people for thousands of years; he
simply saw what theyd been looking at from a dif-
ferent point of view, and by doing so explained the
movements of the planets, the daily rotation of the
earth, and the seasonal changes in the patterns of the
constellations (zodiac).
Before William Harvey, the absence of a visible
connection between arteries and veins prevented peo-
ple from even imagining that the blood could circulate
and, therefore, also made them fail to see the heart as a
pump. Harvey, however, by observing it in a living
creature (a poor pig, tied down with its chest cut open)
saw it as a muscular pump, and by measuring the
volume of its ventricles and calculating the number of
beats per unit of time, he concluded that the blood must
circulate, even though he didnt know how it got from
the arteries to the veins [16].
It had virtually always (in modern times, of course)
been assumed that the vertebrate oocyte grew to ma-
turity as a passive occupant of the ovarian follicle, but
Andy Nalbandov
1
saw it otherwise. The follicular cells
undergo a metamorphic process called luteinization
2

(i.e. formation of the corpus luteum
3
) normally almost
only after the oocyte leaves the follicle at ovulation.
Nalbandov saw this as evidence that the oocyte pre-
vents luteinization. To test this viewpoint he and his
co-workers removed the oocytes from preovulatory
rabbit follicles
4
and found indeed that the follicle cells
luteinized. This finding was the progenitor of a series
of further studies that supported the idea of the oocyte
as an active, indeed essential, factor in the physiology
of its own and its follicles development [17] and,
among other things, led me to write a theoretical paper
on the role of yolk in the evolution of mammalian
viviparity [18]. The absence of yolk in the mammalian
oocyte, in fact, changed my own viewpoint of how rep-

1
Nalbandov, Andrew V. (1912-1986). Andy Nalbandov was one of
L.E. Casidas grad students in the School of Agriculture during the
late 1930s at the same time that I was one of R.K. Meyers grad
students in Zoology at the University of Wisconsin. Andy spent
most of his career at the University of Illinois, Urbana/Champaign
and was one of the founding members of the Society for the Study
of Reproduction. We disagreed about almost everything in
reproduction but remained friends.
2
Luteinization is a term applied to a metamorphosis of the cells of
the post-ovulatory ovarian follicle resulting in the formation of the
corpus luteum, hence the name.
3
Corpus Luteum (pl.: corpora lutea) is an organelle formed from
the post-ovulatory ovarian follicle in most vertebrates. Its secretion
of progesterone is essential for the establishment and, in some
species, the maintenance of pregnancy in mammals.
4
Female rabbits during the breeding season remain in constant
oestrus (heat) and their ovaries always contain a crop of preovu-
latory follicles; these are ovulated only in response to coitus.
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2006 by the Society for the Study of Reproduction, Inc.
tiles may have evolved from amphibians, and mam-
mals from reptiles [18].
The idea of geologic change with time and of the
evolution of different forms of life existed at least two
centuries before Darwin wrote Origin of Species, but it
was the industrial revolution and Malthus writings
about the relation between population growth and food
production that sparked a point of view leading Darwin
to theorize that natural selection was the driving force
of evolution.
Our point of view is by no means always valuable
in our search for knowledge. Columbus, for example,
was so fixed on the idea that he could reach the Far
East by sailing west from Europe that when he did
touch land his fixed viewpoint prevented him from see-
ing that the land and its people in no way resembled
those Marco Polo had described. Even worse, he sailed
around Cuba only enough to convince himself that it
was the peninsula Marco Polo had described. As a
result, he never knew that he had discovered the New
World [19].
Root-Bernsteins Discovering [20] has many more
such examples. Luck or serendipity is often given the
credit for a discovery, even a eureka, but the fact is that
the discoverer saw something that others could have
seen but didnt. The discoverer possessed a mindset
flexible enough to change when confronted with an
oddity, and curious enough to ask the right question:
whats it doing there? Luck helps, but we must be
awake when its offered. This means cultivating as
broad and flexible a point of view as possible [21].
Asking the Right Question
With observations as the meat and potatoes of sci-
ence, its obvious that our appetite and hunger for them
come from our curiosity, our never-ending desire to
know. This is why we begin most (if not all) research
projects with a question and, therefore, why it is so
important that we ask the right one. Troy Duster, I
think, expressed this better than I can in an article in
Science [22]: The procedures for answering any in-
quiry into the empirical world determine the scientific
legitimacy of claims to validity and reliable know-
ledge, but the prior question will always be: Why that
particular question? The first principle of knowledge
construction is, therefore, which question gets asked in
the research enterprise? He then discusses the dangers
of asking the wrong question, using as examples quo-
tations concerning relationships between race and
susceptibility to diseases, responsiveness to therapeutic
drugs, criminal behavior, and warns against the con-
veniences of DNA databases as primary or sole sources
of answers to such questions.
An excellent example of the difference between
asking the wrong question and asking the right one is
Which came first, the chicken or the egg? Because
the question arises from a point of view limited to the
chicken and its egg there is no rational way of
answering it, but from the viewpoint of evolution,
however, there is, i.e. At what stage in the evolution
of the vertebrates did the precursor of the cleidoic
5
egg
appear? We have shifted our focus from the finished
product to its origin, and now have a reasonable chance
of finding an answer.
If the proponents of Intelligent Design could also
shift their focus to origins before asking how complex
designs in nature occur, our kids could learn how we
acquire knowledge without interference from crea-
tionists [8].
Fleming didnt look at his moldy cultures and ask,
How can I get rid of these pesky molds? Instead, he
asked, Why are there no bacteria near the molds?
and penicillin was conceived. Copernicus saw more
than just the sun and earth. Before him, the movements
of the planets and the seasonal changes in fixed stars
were explained only by a crazy-quilt patchwork of
guesses. But, by shifting his point of view from the
earth to the sun and asking, Which of us is moving?
he showed us our solar system. Harvey was equally
ingenious; by looking at a beating heart as a muscle,
and asking Is it behaving like my biceps when I flex
and then extend my arm? he gave us our circulatory
system. If Columbus had asked, How can I be sure
Cuba is a peninsula? and had tried to sail all the way
around it, he would have known he was nowhere near
the Far East.
Theorizing
Making observations of any given subject will in-
evitably generate a question, a hunch, even a theory
about a secret hidden somewhere in the mass of
findings. Darwin, as the naturalist aboard the Beagle
6
,
in the course of collecting samples of fossils, and of
plant and animal life at each port of call, began to
wonder about the meaning of some of themsuch as
evidence of marine animals in sediments on or near
mountaintops. The simplest response to such curiosity
is to accumulate more observations, perhaps more
specialized ones or more oriented toward comparisons.
Darwin continued making observations at home, in-

5
Cleidoic egg: contains all the nutrients, liquid, mRNAs, and pro-
teins necessary for a zygote to complete embryogenesis enclosed in
a protective shell outside the body of its mother. All bird and most
reptile eggs are cleidoic.
6
The Beagle was a ship of the Royal Navy assigned to a circum-
navigation exploration of the globe that lasted for five years.
INDUCTION, DEDUCTION, AND THE SCIENTIFIC METHOD 7



2006 by the Society for the Study of Reproduction, Inc.
cluding the breeding of pigeons, dogs, farm animals,
etc. and eventually was led to theorize that the secret of
biodiversity was evolution through natural selectiona
good example, incidentally, of the use of induction in
science.
The production of milk by mammary glands helped
to distinguish mammals from other vertebrates, but
further observations told us that pigeons also made
milk, although the milk was made of different
components and in their crops, not in mammary
glands. Like mammalian milk, however, pigeon milk
was used to feed their young. Scientists later found that
some species of fish and amphibia produce skin
exudates on which their young feed. Since prolactin, a
pituitary hormone, was already known to stimulate
milk production in both mammals and pigeons, this
knowledge led to speculation that prolactin may be
involved in other forms of maternal behavior. As a
result of research to follow up such ideas, we now
know that prolactin is responsible not only for the skin
exudates in fish and amphibia, it is a necessary, al-
though not always sufficient luteotropin
7
in several
mammalian species, it induces broodiness in birds,
maintains the brooding of young in the skin pouch of
the sea horse, and is a crucial agent in the control of
kidney function in fish that migrate to spawn either
from marine to fresh water or vice versa; these findings
have led to theories about the evolution of prolactin as
a hormone intimately connected with post-fertilization
reproduction, especially the expression and regulation
of maternal behavior in vertebrates [23].
R.K. Meyer
8
, who at the University of Wisconsin
taught me how to become a scientist, used to say half
seriously, I dont theorize; I just experiment and col-
lect facts. However, every one of his facts was both
the offspring and the parent of a theory. It doesnt
matter whether it comes as a question, a hunch, a hypo-
thesis, or a theory as important as or at least similar in
kind to Copernicus heliocentricism; theorizing is what
moves science forward. Theory is more important than
the facts, Einstein told Heisenberg, because theory tells
us what the facts mean [20]. Theory is the most
powerful tool through which we acquire knowledge,

7
A luteotropin is any substance that directly or otherwise increases
the secretion of progesterone by the CL or placenta.
8
Roland Kenneth Meyer earned a Ph.D. in Zoology under
Frederick Hisaw at the University of Wisconsin and returned to its
Zoology Department in the fall of 1935 as its specialist in
endocrinology and reproduction when Hisaw moved to Harvard.
He inherited two grad students left over from Hisaw, but I was his
first full-time grad student. He was a tough but dearly beloved
teacher and researcher. The University of Wisconsins Department
of Zoology has recently initiated a lecture series in his honor.
but like any heavy-duty tool it must be handled with
care and used with discretion.
Like making observations and asking the right
question, theorizing cant be separated from our point
of view. It is here that its value is most seriously
threatened, mainly in three ways. A good theory tends
to block our ability, even our urge, to find a better one.
Of course, the better a theory is the greater is this dan-
ger. A theory can lead to a fixed point of view, several
examples of which Ive mentioned above. The failure
of two labs to discover that the pituitary secretes
prolactin autonomously is an especially pertinent ex-
ample, which I will describe in detail below under
Experimentation since it is also applicable there. Be-
coming emotionally attached to ones theory as though
to a beloved possession is extremely dangerous, for it
can even lead to the temptation to fudge the data. Faith
in the validity of a good theory, however, is another
story (see below).
The theory (or finding) that questions authority.
Proponents of such a theory (which Kuhn would call
the basis of scientific revolution [24]) should have
strong stomachs, stout hearts, and temperaments ag-
gressive enough to enjoy a good fight. Eldredge and
Gould and their theory of punctuated equilibrium [15]
are a good example. The scientific community tends to
be hostile to attacks on authority; whether the theory
persists depends on the effectiveness of the theorys
few supporters in keeping it alive. There is both good
and bad news behind the hostile reception. The good
news is that the resistance of scientists to the ques-
tioning of authority (a peculiar aspect of our skep-
ticism) protects us from the Lysenkos.
9
The bad news
is that the benefits of a superior theory may be delayed
for a very long time, to say nothing of the effect of
such hostility on its proponents.
Copernicus was lucky. He died without knowing
that Tycho Brahe, a contemporary authority of astron-
omy, never accepted his theory or that Galileo suffered
for defending it, or that it was almost one hundred
years before his theory found general acceptance.
Marcelino Sautuola, a Spanish archeologist, died in
disgrace; he was accused in 1875 of forging the cave
paintings of animals and hunts by primitive humans
when the prevailing dogma was that the cave man was
incapable of intellectual creativity [25]. Even Darwins
theory wasnt accepted by most biologists until the

9
Trofim Lysenko was a Russian agronomist who persuaded Stalin
in the 1930s that only the environment, not genes, determined the
quality and productivity of agricultural products. He blocked the
development of genetics in the Soviet Union for more than twenty
years.
8 ROTHCHILD



2006 by the Society for the Study of Reproduction, Inc.
synthesis of genetics and evolution in the mid 1930s
[6, 12], and some scientists even now do not believe in
evolution.
In the mid 1920s, Cecelia Payne-Gaposchkin, as a
graduate student in astronomy at Harvard, found that
her spectroscopic observations of the sun meant that
hydrogen, not iron, was the most common element in
the universe. She described this finding in her Ph.D.
thesis, but it was criticized by Henry Norris Russell,
the worlds authority on stellar spectroscopy as clear-
ly impossible. Several years later Russell published
his own confirmation of her data but without acknow-
ledging her priority. As a graduate student she did not
feel up to defending herself against an authority like
Russell and so added a remark that her data were
probably not real to her Ph.D. thesis. She went on,
however, to a successful career in astronomy. She was
the first woman to become a full professor at Harvard,
and even received the Henry Norris Russell award for
her achievements a few years before she died in 1979
[26].
Defending the controversial theory or finding. The
keystone of Poppers logic of scientific discovery [2] is
falsification, that is, testing the validity of a theory
(or finding) by how well it withstands attempts to
falsify (i.e. disprove) it. The term itself is distasteful
(at least to me) since it implies fake or counterfeit, but
thats beside the point. In the first place, there is
nothing new about his premise. As I said in defining
science, its essence is to doubt without adequate proof,
and the scientists gift of skepticism sees to that. But in
the second and even more important place, disproving
a theory is not the only test of its validity. Any theory
or finding, especially if controversial, always rests on a
fine knife edge, balanced by the weight of disproving
evidence on one side and corroborating evidence on
the other. To claim that only one of these is the only
true test of validity is, to put it very simply, not true
[27].
It is a truism that nothing is sadder than the murder
of a beautiful theory by a nasty little fact. This old
chestnut, however, leaves out the question: How do we
know the fact is a fact? Copernicus could not explain
the movements of the planets as well as did Ptolemy
but his theory explained most of the available facts
much better than did Ptolemys, and he did not aban-
don it. He had assumed that the planets orbits were
perfect circles. Kepler later found them to be elliptical,
and Copernicus faith in his theory was justified. Har-
veys faith in his theory was similarly justified when
capillaries were discovered.
Richard Feynmans and Murray Gelmans theory of
beta decay went against accepted concepts of the inter-
actions between the atomic nucleus and the electrons.
One paper in particular, by a physicist Feynman
respected highly, disproved their theory; but Feynman
waited patiently, and the so-called disproving data
turned out to be erroneous [28].
A somewhat related instance concerns the case of
the oddball fact. Ernest Hooten, the professor of phys-
ical anthropology at Harvard, severely and in public
criticized Sherwood Washburn, his former graduate
student and now a well-established evolutionary an-
thropologist, for failing even to mention Piltdown man
(Eoanthropus dawsonii) in a 1950 paper summarizing
his ideas about human evolution. Washburns point
was that there was something too queer about Piltdown
for him to fit with any of the other known human
fossils. Washburn felt intuitively that Piltdown didnt
belong [29]. It takes guts to take such a scientific gam-
ble. To Hooten, a fact is a fact and we have no right to
decide, without a justifiable reason, to exclude one
from our data. However, in the end, Washburn was
proved to be correct. Four years later, Piltdown was
shown to be a hoax.
The point of these and many similar examples is
that if a theory explains most of what is known better
than any other, a discrepant fact will not kill it unless it
is indeed a fact; even then it may turn out not to be
discrepant. The key to high quality theorizing is Dont
get lost in the details [20]. A good theory, at whatever
level of revolutionary content, will rarely if ever ex-
plain everything. Even Darwin knew that natural selec-
tion did not explain all of evolution. The principle of
theorizing is not necessarily to explain every piece of
information in a particular field of knowledge. It is
intended to explain what is known about the subject
better and more comprehensively than any other has
done so far. Its validity as knowledge depends on its
future history.
Eurekas. When Archimedes leaped from his bath
shouting, I found it! in Greek, he couldnt know that
scientists would love to use his shout to express their
delight in discovering one of natures secrets. I think of
eurekas only as very exciting, big discoveries, but there
is no harm in thinking of them quantitatively as well,
e.g., from barely audible whispers to shouts loud
enough to wake the dead.
The high decibel eureka occupies a special place in
theorizing. No matter what form it comes in, it func-
tions eventually as the basis of a very good theory
pointing to what seem like unlimited possibilities for
exploration. No one knows how such eurekas occur
and it wouldnt make much difference if we did, for
they cant be learned, and are as rare and exciting as
being dealt a straight flush in poker. In the almost
INDUCTION, DEDUCTION, AND THE SCIENTIFIC METHOD 9



2006 by the Society for the Study of Reproduction, Inc.
seventy years since as a graduate student I took my
first step into the world of science, I have had only two
genuine eurekas and consider myself lucky to have had
that many! But just as poker is fun even when we lose,
practicing science is also and for much the same
reasons. Another of R.K. Meyers favorite sayings was
that its those rare little triumphs of discovery that
make science worthwhile, because 99% of being a re-
searcher is drudgery.
Experimentation
Its a common misconception, even among some
scientists, that science means doing nothing but experi-
ments. How can a paleontologist or an archeologist do
an experiment? Experimentation, of course, is a very
useful tool especially for chemists, physicists, and bi-
ologists, but it is not the only tool that even these sci-
entists use. Experimentation is a way of making obser-
vations under controlled conditions, so the value of
such observations is no greater than that of observa-
tions made in the field (including the astronomers) or
in the library, provided that the conditions under which
the observations were made can unquestionably be
identified and compared with one another.
Lets take as an example the prolactin experiments I
used to emphasize the importance of point of view. By
the late 1930s, biologists knew that a pituitary hormone
stimulated the corpus luteum (CL) to secrete proges-
terone, but didnt know which hormone or how its
secretion was regulated. Because mechanical stim-
ulation of the cervix (or sterile coitus) of a rat in heat
induced the secretion of this hormone (which in 1940
turned out to be prolactin), Alel Westman and Dora
Jacobsohn, two Swedish endocrinologists, theorized
that a neural reflex explained the effect of cervical
stimulation. To test this, they cut the connection be-
tween the central nervous system (CNS) and the
pituitary, but when they then stimulated the rats cer-
vix, they were surprised to find that the pituitary still
secreted the as yet unidentified prolactin. They could
not explain why.
Leon Desclin, a Belgian endocrinologist could. To
him, their experiment meant that the pituitary secreted
prolactin independently of the CNS, but in response to
the estrogens secreted when the rat was in heat. To test
his explanation, Desclin transplanted the rats pituitary
beneath the kidney capsule, together with a pellet of
stilbestrol, a synthetic estrogen. Sure enough! The de-
nervated pituitary, when exposed to enough estrogen,
secreted prolactin.
Westman, Jacobsohn, and Desclin were not clumsy,
bumbling amateurs or novices in science, yet they
overlooked one of the most important and obvious
requirements of good experimentation. When we test
the hypothetical solution to a problem by changing the
conditions of the problem itself, we must always be
aware that any of the changes from normal, even a
purely technical one, may account for our results rather
than the particular change we used to test our hypo-
thesis. In both experiments, the researchers point of
view was that the pituitary secreted prolactin only in
response to an external stimulus. Westman and Jacob-
sohn, therefore, did not consider it necessary to see if
denervation alone would induce the secretion of pro-
lactin. Desclin fell into the same trap. It never occurred
to any of them that the secretion of prolactin could be
autonomous. It did occur to John Everett
10
and he
proved it by repeating Desclins experiment without
the estrogen and got the same result [30].
A little later, Everett discovered that if he removed
a rats pituitary completely and transplanted it to the
kidney, the rats CL would maintain a high level of
progesterone secretion for months [31]. In the normal,
intact rat, the CL secretes progesterone for no more
than four weeks. This discovery broadened our under-
standing of the physiology of the CL enormously. It
also emphasizes my remarks on the failed experiment
(see next subheading).
In descriptive sciences like astronomy, paleon-
tology, archeology, etc. where it is impossible to de-
sign experimental changes in the environment being
studied, computer modeling is often used as a form of
experimentation, and lab studies of age, size, and com-
position of collected specimens usually are thought of
as experiments. In any case, the validity of any form of
experimentation depends on whether we ask the right
question, our point of view, the accuracy of our
observations, and the appropriateness of our controls.
The failed experiment. We have a good question
we want answered or a good hypothesis to test; our
experiment was well designed (at least we think so)
and gremlins did not infect our materials and methods,
but the results did not answer our question or support
our hypothesis. The failed experiment can be very
depressing especially if we are a grad student or a
postdocbut even well-seasoned researchers are not
immune to its effect. Nevertheless, the failed experi-
ment should have the opposite effect.
For example, lets look again at the Westman and
Jacobsohn experiment. Their theory of a neural reflex
was correct, but their failure was due to an incorrect

10
John Everett spent most of his scientific career in the De-
partment of Anatomy at Duke University in Durham, N.C. He was
a highly respected researcher; a pioneer in the role of progesterone
in ovulation and in the control of the pituitary by the CNS.
10 ROTHCHILD



2006 by the Society for the Study of Reproduction, Inc.
point of view. The failure itself should have told them
to look for a better one, but it was Everett who looked
and found it. And how much more interesting his dis-
covery turned out to be than what Westman and Jacob-
sohn hoped to find! That is why the failed experiment
should cause elation not depression. Being a researcher
is, after all, playing a never-ending game of solving
fascinating puzzles; the failed experiment tells us that
the excitement of the game is not over, and off we go
again eager for another chance to win.
Perhaps it takes experience to react positively to the
disappointment of the failed experiment, but it is worth
waiting for it to develop.
Publishing
Aside from how publishing papers helps establish
us in our careers by increasing our chances of being
funded, and aside, also, from the sheer fun of telling
our families, colleagues, and friends about our discov-
eries, publishing the results of each of our studies is a
critical test of its validity. There can be a great differ-
ence between our opinion and the opinions of friends,
colleagues, and all those strangers out there about our
studyand we know this! We know that when we sit
down to write our paper we must convince the readers
that our findings are genuine and our interpretation of
them is correct. In the act of satisfying these obliga-
tions with the appropriate words we subject the way
our study was done and how we arrived at our con-
clusions to the ideal test of its validity. Regardless of
any other purpose in writing a paper, this is its indis-
pensable one. Unfortunately, not every scientist, when
writing a paper, seems to know this!
Statistics
Statistical tests have only one purpose in science: to
relieve our anxiety about whether what we have dis-
covered means anything. We cant logically decide
whether something is eternally true; e.g. David Hume
remarked that because the sun has risen every day so
far theres no absolute certainty that it will rise tomor-
row. All our judgments about the validity of a discov-
ery and our ability to predict the result of a given
procedure, therefore, are a matter of probabilities, and
we have a variety of statistical tests we can use to cal-
culate the probabilities. I am far from an expert in this
department. During almost my entire career in experi-
mental science I got by with calculating a standard
error and using either the chi-square or Student t-test,
where necessary. The last two words summarize my
next statement: that probabilities are part of everything
we deal with is not the same as saying that all items of
information must be subjected to a statistical test of
their probability. Statistical tests are too often treated
as if science were a religion and a statistical test a re-
quired ritual in its practice. Thats nonsense. Statistical
tests should be used only as aids in resolving an uncer-
tainty about whether a difference between one con-
dition and another is important.
Recognition
The desire for recognition affects the greats as well
as you and me. Newton and Leibnitz fought bitterly
over who invented calculus; Darwins reaction to the
letter asking his opinion about Wallaces theory of nat-
ural selection is a classic instance of the ubiquity of the
power of recognition.
The question, therefore, isnt whether recognition is
an inseparable part of being a scientist; the question is
how much of a part is it?
Its a matter of our priorities. Recognition brings a
variety of tempting rewards. Turning down an invita-
tion to this or that symposium or to address a plenary
session at an international meeting isnt always easy, to
say nothing of major league stuff like the Nobel Prize,
etc. With all of the varieties of recognition, how do we
handle the flood of requests from grad students and
postdocs to work with us? At what point do we have to
choose between being a good researcher and accepting
so many of its rewards that there is no room left for
being any kind of a researcher? Richard Feynman said
he didnt care about being recognized, and he may
have really meant it, even though he had practically all
the available honors including the Nobel, but he never
stopped being a researcher and never had more than a
handful of protges in his lab.
When weve done good work and received little or
no recognition, it hurts. We can easily imagine how
Mendel must have felt when Nageli, his contemporary
and an authority on inheritance, completely ignored his
findings. Nonetheless, Mendel didnt stop experiments
on how sweet pea flower colors are inherited.
We can also imagine that Aristarchus of Samos
(2nd century BCE), who had the same idea as Coperni-
cus, didnt enjoy being thought of as crazy (except per-
haps by a few of his friends) by all his contemporaries.
In the end, it comes down to this: We become sci-
entists because we want to learn how to solve at least a
few of natures infinite puzzles; if we are recognized
for our accomplishments, so much the better. But
desire for this recognition is not what made us become
scientists. If it is, were in the wrong profession.
ACKNOWLEDGEMENTS
My most sincere thanks to Michael MacGraw,
Chief Reference Librarian of the Cleveland Health Sci-
ences Library, for his assistance in tracking down most
INDUCTION, DEDUCTION, AND THE SCIENTIFIC METHOD 11



2006 by the Society for the Study of Reproduction, Inc.
of the references, and to Rosa Garnett for the many
hours she spent preparing the original and all the
revisions of this essay.

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