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    RBC Inclusions

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    jingx5
    The document describes various developmental organelles and inclusions seen in red blood cells, including Howell-Jolly bodies, basophilic stippling, Pappenheimer bodies, polychromatophilic red cells, and Cabot rings. Abnormal hemoglobin precipitations such as Heinz bodies and hemoglobin H inclusions are also discussed. Finally, the stages of the malaria parasites Plasmodium vivax, P. ovale, P. malariae, and P. falciparum are outlined, with descriptions of ring, trophozoite, schizont, and gametocyte stages for each species.

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    RBC Inclusions

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    jingx5
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    The document describes various developmental organelles and inclusions seen in red blood cells, including Howell-Jolly bodies, basophilic stippling, Pappenheimer bodies, polychromatophilic red cells, and Cabot rings. Abnormal hemoglobin precipitations such as Heinz bodies and hemoglobin H inclusions are also discussed. Finally, the stages of the malaria parasites Plasmodium vivax, P. ovale, P. malariae, and P. falciparum are outlined, with descriptions of ring, trophozoite, schizont, and gametocyte stages for each species.

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    Hematology: Red Blood cell Inclusions

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    The document describes various developmental organelles and inclusions seen in red blood cells, including Howell-Jolly bodies, basophilic stippling, Pappenheimer bodies, polychromatophilic red cells, and Cabot rings. Abnormal hemoglobin precipitations such as Heinz bodies and hemoglobin H inclusions are also discussed. Finally, the stages of the malaria parasites Plasmodium vivax, P. ovale, P. malariae, and P. falciparum are outlined, with descriptions of ring, trophozoite, schizont, and gametocyte stages for each species.

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    Download as docx, pdf, or txt
    1K views6 pages

    RBC Inclusions

    Uploaded by

    jingx5
    The document describes various developmental organelles and inclusions seen in red blood cells, including Howell-Jolly bodies, basophilic stippling, Pappenheimer bodies, polychromatophilic red cells, and Cabot rings. Abnormal hemoglobin precipitations such as Heinz bodies and hemoglobin H inclusions are also discussed. Finally, the stages of the malaria parasites Plasmodium vivax, P. ovale, P. malariae, and P. falciparum are outlined, with descriptions of ring, trophozoite, schizont, and gametocyte stages for each species.

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    of 6

    MTEC 216 Assignment (Midterm) A. Developmental Organelles Inclusions 1.

    Howell Jolly Bodies Description - small, round fragments of the nucleus (resulting from karyorrhexis or nuclear disintegration) of a late nucleated red cell or metarubricyte - stain reddish-blue with Wright stain - may also result from incomplete extrusion of the nucleus or from chromosomes that were separated from the spindle during abnormal mitotic division

    2. Basophilic Stippling

    - fine or coarse, deep blue to purple staining inclusion that appears in erythrocytes on a dried Wright-stained film. - much smaller than Howel-Jolly bodies - usually irregularly shaped - appear homogenously throughout the Hb portion of the erythrocyte represents aggregates of ribosomes that appear during the drying and staining of films.

    3. Pappenheimer Bodies - also called siderotic granules - small, irregular, dark-staining granules that appear near the periphery of a young erythrocyte in a film stained with Wright or supravital stain. - with Perls Prussian blue stain, these bodies stain positively 4. Polychromatophilic Red cells - aka diffusely basophilic red cells - young red cells that no longer have a nucleus but still contain some RNA - as the name implies, this RNA stains diffusely blue with Wright stain - slightly larger cells than mature red cells - contain ribosomes, mitochondria, and other granules

    5. Cabot Rings

    - thin ringlike structures - stain reddish violet in Wright stain - may be circular or appear at the cell periphery, - may form a figure of eight, incomplete rings, or other configurations - may be observed in a nucleated red cell or be associated with stippling or a HowellJolly body in the same erythrocyte

    B. Abnormal Hemoglobin Precipitation Inclusions 1. Heinz Bodies Description - round, refractile inclusions not visible on a Wrightstained film - range in size from about 1 to 3 m - attached to the erythrocyte membrane - best identified by supravital staining with basic dyes such as crystal violet, methylene blue, or brillian cresyl blue 2. Hemoglobin H Inclusions - small greenish-blue inclusion bodies appearing in erythrocytes after 4 drops of blood is incubated with 0.5 mL of 1% brilliant cresyl blue for 20 minutes at 37 0C. - represent precipitated Hb H - can also be seen in early nucleated red cells in the bone marrow of patients with Hb H disease.

    C. Protozoan Inclusions Plasmodium vivax Malaria Inclusions 1. Ring stage

    Appearance of RBC -normal to 1.25x, round; occasionally fine Schffners dots; multiple infection of RBC not uncommon

    Appearance of parasite -large cytoplasm with occasional pseudopods; large chromatin dot

    2. Trophozoite -enlarged 1.5 to 2x; may be distorted; fine Schuffners dots -large amoeboid cytoplasm; large chromatin; fine yellowish-brown pigment

    3. Schizont -enlarged 1.5 to 2x; may be distorted; fine Schffners dots -large, may almost fill RBC; mature = 12 to 24 merozoites; yellowish-brown, coalesced pigment

    4. Gametocyte -Enlarged 1.5 to 2x; may be distorted; fine Schuffners dots -Round to oval; compact; may almost fill RBC; chromatinb compact, eccentric (macrogametocyte) or diffuse (microgametocyte); scattered brown pigment

    Plasmodium ovale Malaria Inclusions 1. Ring Stage

    Appearance of RBC -normal to 1-1/4 X, round to oval; occasionally Schffners dots; occasionally fimbriated; multiple infection of RBC not uncommon

    Appearance of parasite -sturdy cytoplasm; large chromatin

    2. Trophozoite -normal to 1-1/4 X; round to oval; some fimbriated; Schffners dots -compact with large chromatin; dark-brown pigment

    3. Schizont -normal to 1-1/4 X; round to oval; some fimbriated; Schffners dots -mature = 6-14 merozoites with large nuclei, clustered around mass of dark-brown pigment

    4. Gametocyte -normal to 1-1/4 X; round to oval; some fimbriated; Schffners dots -round to oval; compact; may almost fill RBC; chromatin compact, eccentric (macrogametocyte) or more diffuse (micro- gametocyte); scattered brown pigment

    Plasmodium malariae Malaria Inclusion 1. Ring stage

    Description -P. malariae rings have sturdy cytoplasm and a large chromatin dot

    2. Trophozoite -The parasite is smaller than red blood cells and contains 6-12 merozoites, usually 8 in a single ring. The pigment is a brown / greenish central clump. The red cell slightly enlarged, stippled, frequently oval and fimbriated.

    3. Schizont -A schizont containing merozoites (6 to 12) giving a coarse granular appearance.

    4. Gametocyte -The gametocytes of P. malariae have a round shape about the size of red blood cells. They have a fine granular appearance. -Gametocytes contain large amounts of black pigment, with chromatin present as a compact mass in females and diffuse in males. They occupy less than two thirds of the red blood cell.

    Plasmodium falciparum Malaria stage 1. Ring stage

    Description -P. falciparum rings have delicate cytoplasm and one or two small chromatin dots. Rbcs that are infected are not enlarged; multiple infection of rbcs is more common in P. falciparum than in other species. Occasional appliqu forms (rings appearing on the periphery of the rbc) can be present.

    2. Trophozoite -The cytoplasm of mature trophozoites tends to be more dense than in younger rings. As P. falciparum trophozoites grow and mature, t they tend to retain their ring-like shape and sometimes trace amounts of yellow pigment can be seen within the cytoplasm. Growing trophozoites in P. falciparum can appear slightly amoeboid in shape.

    3. Schizont -P. falciparum schizonts are seldom seen in peripheral blood. Mature schizonts have 8 to 24 small merozoites; dark pigment, clumped in one mass.

    4. Gametocyte - P. falciparum gametocytes are crescent or sausage shaped. The chromatin is in a single mass (macrogamete) or diffuse (microgamete).

    References: CDC (n.d.) Retrieved from http://www.cdc.gov/dpdx/resources/pdf/benchAids/malaria/Pfalciparum_benchaidV2.pdf on January 19, 2014 CDC (n.d.) Retrieved from http://www.cdc.gov/dpdx/malaria/dx.html on January 19, 2014 Malaria (n.d.) Retrieved from http://www.phsource.us/PH/PARA/Chapter_9.htm on January 19, 2014 Microscopy Plasmodium species (1999) Retrieved from https://www.msu.edu/course/zol/316/psppscope.htm on January 19, 2014 Parasitology Illustrated (2011) Retrieved from http://parasitologyillustrated.com/classes_of_parasites/protozoa/sporozoa/p_ovale.html on January 16, 2013 Red Blood Cell Inclusions (2009) Retrieved from http://hemeplace.wikispaces.com/file/view/RBC_Inclusions.pdf on January 18, 2014 CDC (n.d.) Retrieved from http://www.cdc.gov/dpdx/resources/pdf/benchAids/malaria/Pfalciparum_benchaidV2.pdf on January 19, 2014

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