Parts of Microscope and Their Functions
Parts of Microscope and Their Functions
Parts of Microscope and Their Functions
Lenses are colour coded, the shortest lens is of the lowest power, and the longest lens is high power lenses. Diaphragm: Diaphragm helps in controlling the amount of light that is passing through the opening of the stage. It is helpful in the adjustment of the control of light that enters. Coarse adjustment knob: Used for focus on scanning. Usually the low power lens is used enabling the movement of the tube. Fine adjustment knob: Used for focus on oil. Moves the body tube for focussing the high power lens. Arm: It supports the tube of the microscope and connects to the base of the microscope. Stage: The platform that is flat used for placing the slides under observation.
The following are the parts of microscope:Stage clip: Stage clips hold the slides in Eyepiece or ocular lens: Eyepiece is the lens, present at the top and is used to see the objects under study. Eyepiece lens contains a magnification of 10X or 15X. Tube: Tube or the body tube, connects the eyepiece to the objective lenses. Resolving nosepiece: It is also known as the Turret. Resolving nosepiece has holders for the different objective lenses. It allows the rotation of the lenses while viewing. Objective lenses: Generally, three or four objective lenses are found on a microscope, with ranges of 10X, 40X, 100X powers. Base: Provides basal support for the microscope. Power switch: The main power switch that turns the illumination on or off. Condensor: The main function of condenser lens is focussing the light on the specimen under observation. When very high powers of 400X are used, condenser lenses are very important. Presence of condenser lens gives a sharper image as compared to the microscope with no condenser lens. proper place.
A standard microscope has three, four, or five objective lenses that range in power from 4X to 100X. When focusing the microscope, be careful that the objective lens doesnt touch the slide, as it could break the slide and destroy the specimen. Specimen or slide: The specimen is the object being examined. Most specimens are mounted on slides, flat rectangles of thin glass. The specimen is placed on the glass and a cover slip is placed over the specimen. This allows the slide to be easily inserted or removed from the microscope. It also allows the specimen to be labeled, transported, and stored without damage. Stage: The flat platform where the slide is placed. Stage clips: Metal clips that hold the slide in place. Stage height adjustment (Stage Control): These knobs move the stage left and right or up and down. Eyepiece: The lens the viewer looks through to see the specimen. The eyepiece usually contains a 10X or 15X power lens. Diopter Adjustment: Useful as a means to change focus on one eyepiece so as to correct for any difference in vision between your two eyes. Body tube (Head): The body tube connects the eyepiece to the objective lenses. Arm: The arm connects the body tube to the base of the microscope. Coarse adjustment: Brings the specimen into general focus. Fine adjustment: Fine tunes the focus and increases the detail of the specimen. Nosepiece: A rotating turret that houses the objective lenses. The viewer spins the nosepiece to select different objective lenses. Objective lenses: One of the most important parts of a compound microscope, as they are the lenses closest to the specimen. Aperture: The hole in the middle of the stage that allows light from the illuminator to reach the specimen. On/off switch: This switch on the base of the microscope turns the illuminator off and on. Illumination: The light source for a microscope. Older microscopes used mirrors to reflect light from an external source up through the bottom of the stage; however, most microscopes now use a lowvoltage bulb. Iris diaphragm: Adjusts the amount of light that reaches the specimen. Condenser: Gathers and focuses light from the illuminator onto the specimen being viewed. Base: The base supports the microscope and its where illuminator is located.