B.tech. Biotechnology Notes

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L . r ' ! cs c r 0 sc(rcn u a n rsrrrc(ttr.nc rg Evenr elativety Uvoy .r sl) NA liagmctr ls !nrlL bnclcrirL gor]li]lrls conl.nrDrilLioN nu0lcolnbs. thc!!!u!!u!t!9ma!qD{ ol and rlorcoll.1i,rsrhoN 246nriLlnnr srncrp!ruachcscoDsistofcuninB !.!!q\ rlicrLtorc.

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nDlccule As ir,lc!rhf dcl.dntr,nrllr)Js rt ollcr Dolscrsiln0rnoushlor siru,le gcnclale mrr.pproac|.sus.rn t, rtrd clonirgslcnro nrany copiesofqch scqNfciir8. DNAntolccules i , r L i f n n tr,l (rl 0 i ' n l \ 1 ,,L j (l ( i r o r ! tr cLhod- iogiDdividud u is,l"r lIl)l,l.s \vil[id otroil fl'Nc. A B!]!rE&lg rfurt\irri,r]nr'(nrrc(lbc ls ir n(ttrtotr\ rrr r r' 1 . .1 . , hr Jr qr l .ofcsotr [cisoLar edlibr ar y l . ! L . . ! t t r r 'l r .(, ' lu' J . , ' - t . . ". 1 ' n .-." ..'-," ." ,-,tru 1 l )i r.r'ol/fJior lar er scqLcncing.Em llsion

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and I'oreca er al. (alsoknown 6 . .i !qi9r!!!, rccenily acquircdby Rocho)and shendure { " ! I 'polory .l o) aUencourland Eenrlj acquiedby Apblied . 'eqLercins.(otrirneruia|ao ,*L llioj: r(r N).Ea Anorl,er merhoLl dr r tf, clonslmplincalion is "bndg. PCR",, Jt, . "rbr *h e t , B g m efl sa -e a n ,p l i fi mu p o ro,,nc' scr kchedr u.soldsqhc,dev;lopdad.* !- ..- '.- dr both Lscd Solexa by (rF* ohnedby ll. ir-).'fheseInerhods prcd(e m&y physicallXji:'.'i!..,"*, q The3ln8l.- 1 q . s u l l r c t l . o co ri u n sw h rcl ru a (h cor r Jii' r liycop,cs!ibsr ql.r r asm en' *l Lto'y{rar$ cofrn.Eitll4d bt. .1. .. drvcl.p6 by skplrcr QLo\.! ltrbu' molccu,jn,erho'l ,

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B'l3liLliltlci|llslsisl
nre to positioos srdmc,lri:i: Onc.clomlDNAscqucoccs p|ysi.alltlocilizod sctaFle ona \i ,o .'" '.q uenc i .'g"pp,oo,h$,r' J J b eo d ,(rd , m . n - r h e DNA r q L e n c e " o f a.ll us c loca(ions,padllcl. SequencinBbysyrdresis".lilie$epopula,dye'leminalion..i in lscs flo$ss oIDNA srndEeh DNAholvmcE*to electophoreric sequemitrg. rLrc by idcnlil'rlt h6cst'cscnr lIc oon!loNcrlary in l)NA nroLcc!lc. Rcverciblc lcrminabr v.rsions rr{hds (Ncdb} llltrnrirM.trd llclioog$c rcversitrlc ofdys*rminarors, dcreclnig nrcEsccnce corespondingth.l posilion, lo ddlns onenucleoridca rirDc, !L rlrtrLcnrovirsllEbloctnrssroupro!llo* 1epolylr$ialionoldotd.ucleodde, | \ rn ro u e n(i ns Alsouscs DNApolynr.arion roodd nucleotides, ore type adding of addedI,o-.d,16 rn(nd(recunc ,t&r r 'yindIhpndnbs oi nucrmLids ,n.l ' In,e. ,, udl .hel ,s nre.l n c J b ] r h e Rlc 6 . o ln r a c h . d p y lo p h o s p h a t e s.T t$ t S( ,r n ( tr cinlht l i c x ri ,tl ]

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