Code No: RR412310 Set No.

1
IV B.Tech I Semester Supplementary Examinations, February 2007
SPECTROSCOPIC ANALYSIS OF BIOMOLECULES
(Bio-Technology)
Time: 3 hours Max Marks: 80
Answer any FIVE Questions
All Questions carry equal marks
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1. Spectroscopic analysis of biomolecules - Justify it. [16]

2. (a) Write the principle involved in IR Spectroscopy.

(b) Explain the different functional groups identification by IR spectra. [8+8]

3. (a) The wavelength of the near I.R. is 0.8µ to 2.5 µ. Calculate the corresponding
frequency in wave numbers.
(b) How IR is useful in the study of nucleosides and nucleotides? [8+8]

4. Discuss the various types of electronic transitions and explain the effect of the
polarity of the solvent on each type of transition. [16]

5. (a) Describe the Woodword and Fischer rules for calculating absorption maximum
in dienes.
(b) Write a note on B-bands. [8+8]

6. Explain with examples about singlet, doublet, triplet and multiplet in NMR spec-
tra. [16]

7. Explain the NMR spectra (Signals, splitting patterns, Chemical shift values) for
the following compounds. [16]

(a) Steric acid (C17 H35 COOH)

(b) Dihydroxy acetone.

8. (a) ESR spectra for spectroscopic analysis of biomolecules - Justify.

(b) Calculate the magnetic field at which the resonance occurs if the g value of
the benzene radical anion is 2.0025 and the ESR spectrometer is operating at
9302 MHz. [8+8]

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Code No: RR412310 Set No. 2
IV B.Tech I Semester Supplementary Examinations, February 2007
SPECTROSCOPIC ANALYSIS OF BIOMOLECULES
(Bio-Technology)
Time: 3 hours Max Marks: 80
Answer any FIVE Questions
All Questions carry equal marks
⋆⋆⋆⋆⋆

1. (a) What is photoelectric effect? Discuss its theory

(b) What are photoelectric cells? Describe their construction and working. [8+8]

2. (a) Explain the different sampling techniques in IR.

(b) Illustrate various types of bending and stretching vibrations that can take
place in triatomic molecules. [8+8]

3. (a) Discuss the IR spectra of Haemoglobin and carboxy peptidase.

(b) Write the limitations of IR spectroscopy. [8+8]

4. (a) Explain the Beer-Lambert principle.

(b) Calculate the absorbance of a concentration 5 × 10−5 M at 440 nm in 5 cm
cell if the molar absorptivity is 3 × 103 L mol−1 cm−1 . [8+8]

5. Discuss the applications of UV Sspectroscopy in qualitative analysis and in quan-

titative analysis. [16]

6. Explain in detail about splitting of the signals. [16]

7. Explain each of the following: [16]

(a) Chemical shift

(b) Spin-spin coupling
(c) Equivalent hydrogens
(d) Up field and Down field.

8. Explain the instrumentation of ESR. [16]

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Code No: RR412310 Set No. 3
IV B.Tech I Semester Supplementary Examinations, February 2007
SPECTROSCOPIC ANALYSIS OF BIOMOLECULES
(Bio-Technology)
Time: 3 hours Max Marks: 80
Answer any FIVE Questions
All Questions carry equal marks
⋆⋆⋆⋆⋆

1. (a) What is meant by dual nature of light?

(b) Define each of the following: absorption, emission, transmission, dispersion,
Scattering and refraction. [4+12]

2. (a) Explain the different sampling techniques in IR.

(b) Illustrate various types of bending and stretching vibrations that can take
place in triatomic molecules. [8+8]

3. Describe the importance of I.R in the research field. [16]

4. Write a detailed account of the various types of transitions involved in the ultra-
violet spectrum. What is the effect of hydrogen bonding on UV absorption? [16]

5. (a) Explain Molar absorptivity and absorbance.

(b) Describe the Woodward-Fischer rules for calculating the absorption maximum
in unsaturated carbonyl compounds. [8+8]

6. (a) Explain in detail about number of signals in NMR Spectra.

(b) Discuss the NMR peaks in diethyl succinate and trimethyl benzene (mesity-
lene). [10+6]

7. Explain each of the following: [16]

(a) Chemical shift

(b) Spin-spin coupling
(c) Equivalent hydrogens
(d) Up field and Down field.

8. (a) How the microwaves are used in ESR spectra.

(b) Write the ESR spectra of benzene, benzene cation and benzene anion. [6+10]

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Code No: RR412310 Set No. 4
IV B.Tech I Semester Supplementary Examinations, February 2007
SPECTROSCOPIC ANALYSIS OF BIOMOLECULES
(Bio-Technology)
Time: 3 hours Max Marks: 80
Answer any FIVE Questions
All Questions carry equal marks
⋆⋆⋆⋆⋆

1. (a) What is photoelectric effect? Discuss its theory

(b) What are photoelectric cells? Describe their construction and working. [8+8]

2. (a) Explain different molecular vibrations in I.R spectroscopy.

(b) How IR spectra is useful to study the biomolecules. [8+8]

3. Explain the different important tips for interpreting an I.R Spectrum. [16]

4. Write a detailed account of the various types of transitions involved in the ultra-
violet spectrum. What is the effect of hydrogen bonding on UV absorption? [16]

5. Describe the different type of chromophores and auxochromes and their study by
using U.V. visible spectrophotometer. [16]

6. (a) Write the importance of NMR spectra.

(b) Calculate the ratio in the Heights of the signals. [6+10]

7. (a) Predict the appearance of high resolution NMR spectrum of acetaldehyde and
acetic acids.
(b) The compound aromatic ester with molecular formula C9 H10 O2 , in the proton
NMR recorded in CDCl3 /TMS showed signals at δ1.2 (t, 3H), δ 2.8 (q, 2H),
δ 7.7 7.8 (m, 5H). Deduce the structure of the compound. [8+8]

8. (a) Sketch the ESRspectrum arising from the interaction of an unpaired electron
with three equivalent protons.
(b) Explain the importance of ESRspectra in the study of Metallo proteins. [8+8]

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