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    Chromatograp Hy: Tranninhg Report ON

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    jaiprakash saini
    This document is a training report on chromatography submitted by Jaiprakash Saini to his supervisor Dr. Arvind Gupta. It discusses different types of chromatography techniques including gas chromatography, column chromatography, thin layer chromatography, high pressure liquid chromatography, ion exchange chromatography, and affinity chromatography. The report describes the basic components and processes of chromatography equipment and provides details on how each chromatography technique separates mixtures based on interactions between a stationary and mobile phase.

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    Chromatograp Hy: Tranninhg Report ON

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    jaiprakash saini
    81 views12 pages
    This document is a training report on chromatography submitted by Jaiprakash Saini to his supervisor Dr. Arvind Gupta. It discusses different types of chromatography techniques including gas chromatography, column chromatography, thin layer chromatography, high pressure liquid chromatography, ion exchange chromatography, and affinity chromatography. The report describes the basic components and processes of chromatography equipment and provides details on how each chromatography technique separates mixtures based on interactions between a stationary and mobile phase.

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    chromatography

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    This document is a training report on chromatography submitted by Jaiprakash Saini to his supervisor Dr. Arvind Gupta. It discusses different types of chromatography techniques including gas chromatography, column chromatography, thin layer chromatography, high pressure liquid chromatography, ion exchange chromatography, and affinity chromatography. The report describes the basic components and processes of chromatography equipment and provides details on how each chromatography technique separates mixtures based on interactions between a stationary and mobile phase.

    Copyright:

    Attribution Non-Commercial (BY-NC)
    Download as PPT, PDF, TXT or read online from Scribd
    Download as ppt, pdf, or txt
    81 views12 pages

    Chromatograp Hy: Tranninhg Report ON

    Uploaded by

    jaiprakash saini
    This document is a training report on chromatography submitted by Jaiprakash Saini to his supervisor Dr. Arvind Gupta. It discusses different types of chromatography techniques including gas chromatography, column chromatography, thin layer chromatography, high pressure liquid chromatography, ion exchange chromatography, and affinity chromatography. The report describes the basic components and processes of chromatography equipment and provides details on how each chromatography technique separates mixtures based on interactions between a stationary and mobile phase.

    Copyright:

    Attribution Non-Commercial (BY-NC)
    Download as PPT, PDF, TXT or read online from Scribd
    Download as ppt, pdf, or txt
    of 12

    TRANNINHG REPORT

    ON

    chromatograp
    hy
    Under Supervision Submitted by
    Dr. Arvind Gupta Jaiprakash Saini
    (Senior scientist) B.sc Biotech(Int.)3ed year

    Submitted to
    Lords International collage Chikani, Alwar
    Chromatography is the
    separation of a mixture into
    individual components using
    a stationary and a mobile
    phase.
    Chromatography Equipment

    1. Solvent Tank
    2. Pump
    3. Feed injection
    4. Oven
    5. Detector
    1. GAS-LIQUID
    CHROMATOGRAPHY
    2.COLUMN CHROMATOGRAPHY
    3.THIN LAYER
    CHROMATOGRAPHY
    4.HIGH PRESSURE LIQUID
    CHROMATOGRAPHY
    5.ION EXCHANGE
    CHROMATOGRAPHY
    Gas- liquid Chromatography Which
    the mobile phase is carrier gas
    Stationary phase is a microscopic
    liquid on an inert solid support, inside
    glass or metal tubing called a Colum.
    And this instrument used to perform
    gas Chromatographic separations is
    called a gas Chromatography
    (TLC) is a chromatography
     technique used to separate
    mixtures. Thin layer
    chromatography is performed on
    a sheet of glass, plastic, or
    aluminum foil, which is coated
    with a thin layer of adsorbent
     material, usually silica gel,
    aluminium oxide, or cellulose.
    This layer of adsorbent is known
    as the stationary phase.
    •High performance liquid
    chromatography (or high
    pressure liquid
    chromatography, HPLC) is a
    form of column chromatography
     used frequently inbiochemistry
     and analytical chemistry to
    separate, identify, and quantify
    compounds based on their
    idiosyncratic polarities and
    interactions with the column's
    stationary phase.
    •Ion-exchange chromatography (or ion
    chromatography) is a process that allows the
    separation of ions and polar molecules
     based on their charge. It can be used for
    almost any kind of charged molecule
    including large proteins, small nucleotides
     and amino acids. The solution to be injected
    is usually called a sample, and the
    individually separated components are
    called analytes. It is often used in protein
    purification, water analysis, and quality
    control.
    Affinity chromatography is
    a method of
    separating biochemical mixtu
    res, based on a highly
    specific biological interaction
    such as that between antigen
    and antibody, enzyme
    and substrate,
    or receptor and ligand

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