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    Pglo Write Up

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    The student conducted an experiment transforming E. coli bacteria with a pGLO plasmid containing genes for GFP fluorescence and ampicillin resistance. When the transformed bacteria were grown on an ampicillin plate, they did not fluoresce or grow, indicating successful transformation. On a plate with ampicillin and arabinose, the bacteria grew large and yellow with fluorescence, showing plasmid gene expression. The experiment demonstrated how external factors like antibiotics and sugars can control bacterial gene expression through transformation of exogenous DNA.

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    Pglo Write Up

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    The student conducted an experiment transforming E. coli bacteria with a pGLO plasmid containing genes for GFP fluorescence and ampicillin resistance. When the transformed bacteria were grown on an ampicillin plate, they did not fluoresce or grow, indicating successful transformation. On a plate with ampicillin and arabinose, the bacteria grew large and yellow with fluorescence, showing plasmid gene expression. The experiment demonstrated how external factors like antibiotics and sugars can control bacterial gene expression through transformation of exogenous DNA.

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    The student conducted an experiment transforming E. coli bacteria with a pGLO plasmid containing genes for GFP fluorescence and ampicillin resistance. When the transformed bacteria were grown on an ampicillin plate, they did not fluoresce or grow, indicating successful transformation. On a plate with ampicillin and arabinose, the bacteria grew large and yellow with fluorescence, showing plasmid gene expression. The experiment demonstrated how external factors like antibiotics and sugars can control bacterial gene expression through transformation of exogenous DNA.

    Copyright:

    © All Rights Reserved
    Download as DOCX, PDF, TXT or read online from Scribd
    Download as docx, pdf, or txt
    109 views3 pages

    Pglo Write Up

    Uploaded by

    api-317634731
    The student conducted an experiment transforming E. coli bacteria with a pGLO plasmid containing genes for GFP fluorescence and ampicillin resistance. When the transformed bacteria were grown on an ampicillin plate, they did not fluoresce or grow, indicating successful transformation. On a plate with ampicillin and arabinose, the bacteria grew large and yellow with fluorescence, showing plasmid gene expression. The experiment demonstrated how external factors like antibiotics and sugars can control bacterial gene expression through transformation of exogenous DNA.

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    © All Rights Reserved
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    Download as docx, pdf, or txt
    of 3

    Conor Wight

    Mrs. Lyon
    AP Biology
    1/28/2016
    pGLO Transformation write up
    Introduction
    In this experiment, plasmids, promoters, antibiotics and
    antibiotic resistance were all applied in order to achieve the result of
    transformation. A plasmid is a small, circular piece of DNA that
    comprises of genes that control one or more traits that can have a
    positive impact on a bacterias ability to survive. In this experiment
    specifically, the pGLO plasmid was analyzed. pGLO encodes the gene
    for both a GFP and a gene for resistance to antibiotic ampicillin. As the
    name suggests, an antibiotic is used to inhibit the growth of and/or
    destroy bacteria, which makes the benefit of the pGLO plasmid obvious
    in the face of ampicillin. In addition to resisting antibiotics, pGLO
    employs a gene regulation system in which a promoter, a sugar called
    arabinose, is used to control the expression of the fluorescent protein
    (GFP) in transformed cells. All of these factors contribute to the
    transformation of the E. Coli bacteria. Transformation is the action of
    inserting a gene into an organism in order to alter or create a
    phenotype in a given organism. In this case, the E. Coli bacteria was
    transformed in order to give it the ability to produce bioluminescence,
    the same trait found in many jellyfish, among other organisms.
    Petri Dish Physical Descriptions
    Petri Dish
    Colour
    Growth
    +pGLO
    White, no
    Large
    LB/amp
    glow

    Pictures

    +pGLO
    LB/amp/ara

    Yellow, glow

    Large

    -pGLO LB/amp Yellow, glow

    Growth*

    -pGLO LB

    Lawn
    growth

    Yellow, no
    glow

    *No growth should have occurred as the ampicillin was accidentally


    killed off, making conditions identical to the -pGLO LB plate
    Transformation was successful. This is due to the fact that the
    coloration of the bacteria became white whe n the plasmid and
    ampicillin was added to the bacteria, while the bacteria in the second
    petri dish exhibited similar coloration and the trait to glow under a UV
    light as a result of the arabinose sugar. Both of these were the desired
    phenotypes from the attempted, and successful, transformation.
    This lab could be made more successful with more trials being
    run, as well as the possibility of better backdrops and/or lighting in
    order to truly see the desired colors, as each plate looked relatively the
    same to the unprofessional eye. This would have also eliminated the
    inevitable human error over the timing of the heat shocking of the
    plasmid.
    Organisms stand to gain from the ability to turn a gene on or off
    in the face of varying conditions. One such example of this would be in
    the realm of camouflage; the ability to turn a gene on in order to

    match the coloration of an organisms surroundings is advantageous


    for both predators and prey.
    One potential application of transforming bacteria exists in
    medicine, a field in which bacteria can be genetically altered to assist
    humans against illness. If transformed with the correct genes, bacteria
    can be applied within medicines and vaccinations in order to stop
    diseases and viruses from afflicting humans and other organisms.

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