Alleleustrious pmWasabi-Cx43

Catalog Number: Introduction All ustrious pmWasabi-Cx43 is a mammalian expression vector that encodes a fusion of rat alpha-1 connexin-43 (protein Cx43, gene Gja1) N-terminal to mWASABI. It can be a great tool for your research on gap junctions. All ustrious mWasabi is a monomeric green fluorescent protein that can be easily detected using standard GFP filter sets. mWasabi may be used as a direct replacement for EGFP or other GFPs for superior performance, and may be co-imaged with blue and red fluorescent labels without substantial bleed-through. Connexins are gap junction proteins that assemble to form 6-member hemichannels in vertebrates. These structures can move around freely within the lipid bilayer and with help from specific proteins, mainly cadherins, the hemichannels are able to dock with hemichannels of adjacent cells, forming gap junctions. Connexin protein family members are diverse, Cx43 refers to the protein with 43 kD molecular weight. It is a gap junction component involved in cell-cell communications.
ele ele

ABP-FP-WCX43

Source Engineered variant of mTFP1, originally derived from Clavularia sp. coral. Recommended Use mWasabi has been optimized for use with standard GFP/FITC filter sets. Features About 2-fold brighter than EGFP Similar photostability to EGFP Uses standard filter sets Can be co-imaged with blue and red FPs or dyes Mammalian expression vector ready to transfect your favorite cells Low sensitivity to acidic pH (fluorescence pKa=4.3) True monomer that will not aggregate or cause nonspecific interactions

Reconstitution 5 g provided in lyophilized powder form. Reconstitute with 5 L of nuclease-free water for a final concentration of 1 g/L. Storage Store at -20C or at -80C for long-term preservation.

Human CMV Immediate-Early Promoter (CMV Promoter)........1-589 Cx43............................................824-1969 Linker......1970-1990 Wasabi.1991-2701 SV40 PolyA Signal.....................2853-2903 bla Promoter...............................3442-3546 Ampicillin Resistance Gene......3531-4391 pUC Origin..................................4540-5182 Upstream Sequencing Primer: Universal CMV Promoter Primer Downstream Sequencing Primer: SV40 Primer:GCTTT ATTTG TGAAA TTTGT GATGC TATTG C BLA SV40 Poly A Wasabi AMPR pUC Ori PCMV

pmWasabiCx43 5264bp

Cx43

Linker

References: Ai H, Olenych SG, Wong P, Davidson MW, Campbell RE. Hue-shifted monomeric variants Clavularia cyan fluorescent protein: identification of the molecular
determinants of color and applications in fluorescence imaging. BMC Biology. 2008 Mar; 6:13. Shaner NC, Patterson GH, Davidson MW. Advances in fluorescent protein technology. J Cell Sci. 2007 Dec 15;120(Pt 24):4247-60. Ai HW, Hazelwood KL, Davidson MW, Campbell RE. Fluorescent protein FRET pairs for ratiometric imaging of dual biosensors. Nature Methods. 2008 5(5): 401-03. Ai HW, Henderson JN, Remington SJ, Campbell RE. Directed evolution of a monomeric, bright, and photostable version of Clavularia cyan fluorescent protein: structural characterization and applications in fluorescence imaging. Biochem J. 2006. Shaner NC, Steinbach PA, Tsien RY. A guide to choosing fluorescent proteins. Nat Methods. 2005 2(12):905-09. Saffitz JE, Laing JG, Yamada KA. "Connexin expression and turnover : implications for cardiac excitability." Circ. Res. 2000 86 (7): 7238. Aronica E, Gorter J, Jansen G et al. "Expression of connexin 43 and connexin 32 gap-junction proteins in epilepsy-associated brain tumors and in the perilesional epileptic cortex". Acta Neuropathol. 2001 101 (5): 44959.

Allele Biotechnology and Pharmaceuticals 9924 Mesa Rim Road San Diego, CA 92121 Tel: (800) 991-RNAi (858) 587-6645 Fax: (858) 587-6692 [email protected] Copyright 2008-2010

Alleleustrious pmWasabi-Cx43
Sequence
1 61 121 181 241 301 361 421 481 541 601 661 721 781 841 901 961 1021 1081 1141 1201 1261 1321 1381 1441 1501 1561 1621 1681 1741 1801 1861 1921 1981 2041 2101 2161 2221 2281 2341 2401 2461 2521 2581 2641 2701 2761 2821 2881 2941 3001 3061 3121 3181 3241 3301 3361 3421 3481 3541 3601 3661 3721 3781 3841 3901 3961 4021 4081 4141 4201 4261 4321 4381 TAGTTATTAA CGTTACATAA GACGTCAATA ATGGGTGGAG AAGTACGCCC CATGACCTTA CATGGTGATG ATTTCCAAGT GGACTTTCCA ACGGTGGGAG CCGGACTCAG AAGGCGTGAG GAGCCTGAAC GCGCCGGCTT CTTGGGGAAG GTCAGTGCTC TGATGAACAG TGACAAGTCC TGTGCCCACA AAACAAGAAA CCTGAAGCAG AATGAGGGGC GGTGGCCTTC CTGCAAGAGA AACCATCTTC TGAGCTCTTC TTACCACGCC CTACTTCAAT CAAGCTGGTT CGAGCAAAAC CATCTCCAAC AGTTGCTGCT AGCCAGCAGC GGTCGCCACC GAAGATCAAG GGGCGAGGGC CCCCCTGCCC CACCAAGTAC TTGGGAGCGC CATGGAGGAG CGGCCCCGTG GCGCGACGGC CCACCGCGTT TCACTTTGTG CGTTTACGAG AGCGGCCGCG TAAAAAACCT TTAACTTGTT CAAATAAAGC CTTAACGCGT ACCGCTACAC GCCACGTTCG TTTAGTGCTT GGGCCATCGC AGTGGACTCT TTATAAGGGA TTTAACGCGA ATGTGCGCGG TGAGACAATA AACATTTCCG ACCCAGAAAC ACATCGAACT TTCCAATGAT CCGGGCAAGA CACCAGTCAC CCATAACCAT AGGAGCTAAC AACCGGAGCT TGGCAACAAC AATTAATAGA CGGCTGGCTG TTGCAGCACT GTCAGGCAAC AGCATTGGTA TAGTAATCAA CTTACGGTAA ATGACGTATG TATTTACGGT CCTATTGACG TGGGACTTTC CGGTTTTGGC CTCCACCCCA AAATGTCGTA GTCTATATAA ATCTCGAGCT GAAAGTACCA TCTCATTTTT CACTTTCATT CTTCTGGACA TTCATATTCA TCTGCCTTTC TTCCCCATCT CTCCTGTACT GAAGAGGAGC ATTGAAATCA GGCTTGCTGA CTGCTCATCC GATCCCTGCC ATCATCTTCA TACGTCTTCT ACCACTGGCC GGCTGCTCCT ACTGGTGACA TGGGCGAACT TCGCACGCCC GGACATGAAC CGCGCCAGCA ATGGTGAGCA CTGAAGATGG AAGCCCTACG TTCTCCTACG CCCGACGACA ACCATGACCT GACTCCTTCA ATGCAGAAGG GTGCTGAAGG GACTTCAAGA GACCACCGCA ATCGCCGTGG ACTCTAGATC CCCACACCTC TATTGCAGCT ATTTTTTTCA CCTGTAGCGG TTGCCAGCGC CCGGCTTTCC TACGGCACCT CCTGATAGAC TGTTCCAAAC TTTTGCCGAT ATTTTAACAA AACCCCTATT ACCCTGATAA TGTCGCCCTT GCTGGTGAAA GGATCTCAAC GAGCACTTTT GCAACTCGGT AGAAAAGCAT GAGTGATAAC CGCTTTTTTG GAATGAAGCC GTTGCGCAAA CTGGATGGAG GTTTATTGCT GGGGCCAGAT TATGGATGAA ACTGTCAGAC TTACGGGGTC ATGGCCCGCC TTCCCATAGT AAACTGCCCA TCAATGACGG CTACTTGGCA AGTACATCAA TTGACGTCAA ACAACTCCGC GCAGAGCTGG CAAGCTTCGA AACAGCAGCA CCTTTGACTT AAGTGAAAGA AGGTCCAAGC GAATCCTGCT GCTGTAACAC CTCACGTGCG TGGCCCATGT TCAAAGTGGC AGAAGTTCAA GAACCTACAT AGTGGTACAT CCCACCAGGT TGCTGGTGGT TCAAAGGCGT CACTGAGCCC CACCAACGGC GAAACAATTC ACAGCGCAGA AGCCGTTCGA TCCAGCCATT GCAGGCCTCG AGGGCGAGGA AGGGCAACGT ACGGCACCAA ACATTCTGAC TCCCCAACTA TCGAGGACAA TCTACGAGAT AGACCACCGG GCGACGTCAA CCATCTACAG TCGAGATCCT CCCGCAACTC ATAATCAGCC CCCCTGAACC TATAATGGTT CTGCATTCTA CGCATTAAGC CCTAGCGCCC CCGTCAAGCT CGACCCCAAA GGTTTTTCGC TGGAACAACA TTCGGCCTAT AATATTAACG TGTTTATTTT ATGCTTCAAT ATTCCCTTTT GTAAAAGATG AGCGGTAAGA AAAGTTCTGC CGCCGCATAC CTTACGGATG ACTGCGGCCA CACAACATGG ATACCAAACG CTATTAACTG GCGGATAAAG GATAAATCTG GGTAAGCCCT CGAAATAGAC CAAGTTTACT ATTAGTTCAT TGGCTGACCG AACGCCAATA CTTGGCAGTA TAAATGGCCC GTACATCTAC TGGGCGTGGA TGGGAGTTTG CCCATTGACG TTTAGTGAAC ATTCGGCAGC GACTTTTAAA CAGCCTCCAA GAGGTGCCCA CTACTCCACC CCTGGGGACA TCAACAACCT CTTCTGGGTC GTTCTATGTG CCAGACTGAC GTACGGGATT CATCAGCATC CTATGGGTTC AGACTGCTTC GTCCTTGGTG TAAGGATCGC ATCAAAAGAC TCCACTCTCG CTCGTGCCGC GCAAAATCGC TTTCCCCGAC AGCCATCGTG GCCTGATGAC GACCACAATG GAATGGCCAC CACCATCAAC CACCGCGTTC CTTCAAGCAG GGGCATCGTG ACACCTCAAG CTGGGACGCC GATGAAGCTG GGCCAAGAAG GAACCACGAC CACCGACGGC ATACCACATT TGAAACATAA ACAAATAAAG GTTGTGGTTT GCGGCGGGTG GCTCCTTTCG CTAAATCGGG AAACTTGATT CCTTTGACGT CTCAACCCTA TGGTTAAAAA CTTACAATTT TCTAAATACA AATATTGAAA TTGCGGCATT CTGAAGATCA TCCTTGAGAG TATGTGGCGC ACTATTCTCA GCATGACAGT ACTTACTTCT GGGATCATGT ACGAGCGTGA GCGAACTACT TTGCAGGACC GAGCCGGTGA CCCGTATCGT AGATCGCTGA CATATATACT AGCCCATATA CCCAACGACC GGGACTTTCC CATCAAGTGT GCCTGGCATT GTATTAGTCA TAGCGGTTTG TTTTGGCACC CAAATGGGCG CGTCAGATCC ACTTTTCTTT CTTTAAACAG GGAGTTCCAC GACATGGGTG GCTGGAGGGA GCTGTTGAGT GGCTGCGAAA CTTCAGATCA ATGAGGAAGG GGGGTCAACG GAAGAGCACG CTCTTCAAGT AGCTTGAGCG CTCTCACGTC TCTCTCGCTT GTGAAGGGAA TGCGGATCTC CCTATGTCTC AATTACAACA ATGGGGCAGG GACAACCAGA GACCAACGAC CTGGAGATTG GGCGTAATCA GCCTTCGTGA CTGGAGGTGA AGTTACGGCA TCCTTCCCCG AAGGTGAAGT GGCGAGAACT TCCACCGAGA CTGCTGGAGG GCGGTGAAGC AAGGACTACA ATGGACGAGC TGTAGAGGTT AATGAATGCA CAATAGCATC GTCCAAACTC TGGTGGTTAC CTTTCTTCCC GGCTCCCTTT AGGGTGATGG TGGAGTCCAC TCTCGGTCTA ATGAGCTGAT AGGTGGCACT TTCAAATATG AAGGAAGAGT TTGCCTTCCT GTTGGGTGCA TTTTCGCCCC GGTATTATCC GAATGACTTG AAGAGAATTA GACAACGATC AACTCGCCTT CACCACGATG TACTCTAGCT ACTTCTGCGC GCGTGGGTCT AGTTATCTAC GATAGGTGCC TTAGATTGAT TGGAGTTCCG CCCGCCCATT ATTGACGTCA ATCATATGCC ATGCCCAGTA TCGCTATTAC ACTCACGGGG AAAATCAACG GTAGGCGTGT GCTAGCGCTA CATTGGGGGG ACAGGTCTGA CAACTTTGGC ACTGGAGTGC AGGTGTGGCT CAGCTTGGGG ACGTCTGCTA TATTCGTGTC AAGAGAAGCT TGGAGATGCA GCAAGGTGAA CTGTCTTCGA CGGTCTACAC CCACGGAGAA TGAACATCAT GAAGCGATCC CAAAATACGC CTCCTGGGTA AGCAAGCTAG CCGGAAGCAC ATGCCAAAAA CTTCCAGCAG CGGATCCACC AGCCCGACAT TCGAGGGCGA AGGAGGGAGC ACAGGGCCTT AGGGCTACTC CCGACATCTC TCCCCCCCAA GGATGTACGT GCGGCGGCCA TGCCCGACTA ACAAGGTGAC TGTACAAGTA TTACTTGCTT ATTGTTGTTG ACAAATTTCA ATCAATGTAT GCGCAGCGTG TTCCTTTCTC AGGGTTCCGA TTCACGTAGT GTTCTTTAAT TTCTTTTGAT TTAACAAAAA TTTCGGGGAA TATCCGCTCA ATGAGTATTC GTTTTTGCTC CGAGTGGGTT GAAGAACGTT CGTATTGACG GTTGAGTACT TGCAGTGCTG GGAGGACCGA GATCGTTGGG CCTGTAGCAA TCCCGGCAAC TCGGCCCTTC CGCGGTATCA ACGACGGGGA TCACTGATTA TTAAAACTTC

Allele Biotechnology and Pharmaceuticals 9924 Mesa Rim Road San Diego, CA 92121 Tel: (800) 991-RNAi (858) 587-6645 Fax: (858) 587-6692 [email protected] Copyright 2008-2010

Alleleustrious pmWasabi-Cx43
4441 4501 4561 4621 4681 4741 4801 4861 4921 4981 5041 5101 5161 5221 ATTTTTAATT CTTAACGTGA CTTGAGATCC CAGCGGTGGT TCAGCAGAGC TCAAGAACTC CTGCCAGTGG AGGCGCAGCG CCTACACCGA GGAGAAAGGC AGCTTCCAGG TTGAGCGTCG ACGCGGCCTT CGTTATCCCC TAAAAGGATC GTTTTCGTTC TTTTTTTCTG TTGTTTGCCG GCAGATACCA TGTAGCACCG CGATAAGTCG GTCGGGCTGA ACTGAGATAC GGACAGGTAT GGGAAACGCC ATTTTTGTGA TTTACGGTTC TGATTCTGTG TAGGTGAAGA CACTGAGCGT CGCGTAATCT GATCAAGAGC AATACTGTTC CCTACATACC TGTCTTACCG ACGGGGGGTT CTACAGCGTG CCGGTAAGCG TGGTATCTTT TGCTCGTCAG CTGGCCTTTT GATAACCGTA TCCTTTTTGA CAGACCCCGT GCTGCTTGCA TACCAACTCT TTCTAGTGTA TCGCTCTGCT GGTTGGACTC CGTGCACACA AGCTATGAGA GCAGGGTCGG ATAGTCCTGT GGGGGCGGAG GCTGGCCTTT TTACCGCCAT TAATCTCATG AGAAAAGATC AACAAAAAAA TTTTCCGAAG GCCGTAGTTA AATCCTGTTA AAGACGATAG GCCCAGCTTG AAGCGCCACG AACAGGAGAG CGGGTTTCGC CCTATGGAAA TGCTCACATG GCAT ACCAAAATCC AAAGGATCTT CCACCGCTAC GTAACTGGCT GGCCACCACT CCAGTGGCTG TTACCGGATA GAGCGAACGA CTTCCCGAAG CGCACGAGGG CACCTCTGAC AACGCCAGCA TTCTTTCCTG

Enzyme AatI AatII Acc113I Acc16I AccB1I AccB7I AccBSI AccI AcsI AcyI AfeI AflIII AgeI AhdI Alw21I Alw44I AlwNI Ama87I Aor51HI ApaLI ApoI AscI AseI AsnI Asp700I AspEI AspHI AspI AtsI AvaI AviII BalI BamHI BanI BanII BbiII BbrPI BbsI Bbv12I Bbv16II BcgI BcoI BglI BglII BpiI BpmI Bpu14I BpuAI BsaAI BsaBI BsaHI BsaI BsaMI BsaOI BsaWI Bse118I Bse8I BsePI BseRI Bsh1285I Bsh1365I BshNI BsiEI BsiHKAI BsiI BsmI BsoBI

Cuts 1 5 1 1 5 1 2 2 4 7 1 2 1 1 8 2 1 2 1 2 4 1 2 2 2 1 8 2 2 2 1 1 1 5 6 7 1 2 8 2 1 2 5 1 2 7 1 2 4 2 7 3 3 5 5 5 2 1 5 5 2 5 5 8 3 3 2

Positions 1945 125 178 261 447 2487 3837 4095 465 813 2123 3134 4365 2430 3034 3479 1374 1410 629 2873 3358 3369 122 175 258 444 2436 2484 3778 596 2945 5206 1978 4318 620 909 1161 1248 1506 3650 3735 4896 3646 4892 4797 613 2267 596 3646 4892 629 2873 3358 3369 779 7 4143 7 4143 1705 3718 4318 620 909 1161 1248 1506 3650 3735 4896 860 1181 860 1181 613 2267 4095 2076 1972 465 813 2123 3134 4365 620 1161 1506 1589 2161 3104 122 175 258 444 2436 2484 3778 1045 1318 1519 620 909 1161 1248 1506 3650 3735 4896 1318 1519 3802 613 2267 90 212 283 2558 4200 609 1318 1519 837 887 1885 1966 2146 2509 4233 627 1318 1519 340 1045 2458 3175 1214 2721 122 175 258 444 2436 2484 3778 2014 2425 4251 1854 2809 2908 1983 2706 3800 3949 4872 600 1978 4022 4853 5000 783 1978 2426 3070 4233 1214 2721 779 1159 1641 1673 2011 2350 1983 2706 3800 3949 4872 1214 2721 465 813 2123 3134 4365 1983 2706 3800 3949 4872 620 909 1161 1248 1506 3650 3735 4896 1716 3654 5038 1854 2809 2908 613 2267

Sequence agg/cct gacgt/c agt/act tgc/gca g/gyrcc ccannnn/ntgg gagcgg gt/mkac r/aatty gr/cgyc agc/gct a/crygt a/ccggt gacnnn/nngtc gwgcw/c g/tgcac cagnnn/ctg c/ycgrg agc/gct g/tgcac r/aatty gg/cgcgcc at/taat at/taat gaann/nnttc gacnnn/nngtc gwgcw/c gacn/nngtc gacn/nngtc c/ycgrg tgc/gca tgg/cca g/gatcc g/gyrcc grgcy/c gr/cgyc cac/gtg gaagac gwgcw/c gaagac cgannnnnntgc c/ycgrg gccnnnn/nggc a/gatct gaagac ctggag tt/cgaa gaagac yac/gtr gatnn/nnatc gr/cgyc ggtctc gaatgc cgry/cg w/ccggw r/ccggy gatnn/nnatc g/cgcgc gaggag cgry/cg gatnn/nnatc g/gyrcc cgry/cg gwgcw/c ctcgtg gaatgc c/ycgrg

Bsp119I Bsp1407I Bsp143II Bsp19I BspCI BspHI BspLU11I BspMI BsrBI BsrBRI BsrDI BsrFI BsrGI BssAI BssHII BssSI BssT1I BstBI BstD102I BstDSI BstEII BstH2I BstI BstMCI BstPI BstSFI BstSNI BstX2I BstXI BstYI BstZI CciNI Cfr10I CfrI Csp45I DraI DraII DraIII DrdI DsaI EaeI EagI Eam1104I Eam1105I EarI Ecl136II EclHKI EclXI Eco105I Eco130I Eco147I Eco24I Eco255I Eco31I Eco47III Eco52I Eco57I Eco64I Eco72I Eco88I Eco91I EcoICRI EcoO109I EcoO65I EcoRI EcoT14I EcoT22I

1 627 1 2691 4 598 3020 3028 4966 3 360 1989 2340 1 3949 2 3478 4486 1 5206 1 1384 2 3034 3479 2 1214 2721 2 4082 4264 5 783 1978 2426 3070 4233 1 2691 5 783 1978 2426 3070 4233 1 779 3 1716 3654 5038 6 360 757 840 1473 1989 2340 1 627 2 3034 3479 5 360 1431 1989 2340 2655 1 2635 4 598 3020 3028 4966 1 1972 5 1983 2706 3800 3949 4872 1 2635 6 1759 2545 2952 4072 4750 4941 1 340 12 585 609 1390 1604 1972 2604 3671 3688 4456 4468 4554 4565 1 2140 12 585 609 1390 1604 1972 2604 3671 3688 4456 4468 4554 4565 1 2703 1 2703 5 783 1978 2426 3070 4233 4 2074 2515 2703 3925 1 627 6 697 704 2761 3740 4432 4451 2 1057 2214 2 2587 3178 2 3222 5104 5 360 1431 1989 2340 2655 4 2074 2515 2703 3925 1 2703 7 913 1135 1156 1246 1306 1510 3529 1 4318 7 913 1135 1156 1246 1306 1510 3529 3 618 1159 1504 1 4318 1 2703 1 340 6 360 757 840 1473 1989 2340 1 1945 6 620 1161 1506 1589 2161 3104 1 3837 3 2014 2425 4251 1 596 1 2703 7 753 1066 1207 2056 2479 3636 4684 5 465 813 2123 3134 4365 1 1045 2 613 2267 1 2635 3 618 1159 1504 2 1057 2214 1 2635 1 629 6 360 757 840 1473 1989 2340 1 5263

tt/cgaa t/gtaca rgcgc/y c/catgg cgat/cg t/catga a/catgt acctgc gagcgg gatnn/nnatc gcaatg r/ccggy t/gtaca r/ccggy g/cgcgc ctcgtg c/cwwgg tt/cgaa gagcgg c/crygg g/gtnacc rgcgc/y g/gatcc cgry/cg g/gtnacc c/tryag tac/gta r/gatcy ccannnnn/ntgg r/gatcy c/ggccg gc/ggccgc r/ccggy y/ggccr tt/cgaa ttt/aaa rg/gnccy cacnnn/gtg gacnnnn/nngtc c/crygg y/ggccr c/ggccg ctcttc gacnnn/nngtc ctcttc gag/ctc gacnnn/nngtc c/ggccg tac/gta c/cwwgg agg/cct grgcy/c agt/act ggtctc agc/gct c/ggccg ctgaag g/gyrcc cac/gtg c/ycgrg g/gtnacc gag/ctc rg/gnccy g/gtnacc g/aattc c/cwwgg atgca/t

Allele Biotechnology and Pharmaceuticals 9924 Mesa Rim Road San Diego, CA 92121 Tel: (800) 991-RNAi (858) 587-6645 Fax: (858) 587-6692 [email protected] Copyright 2008-2010

Alleleustrious pmWasabi-Cx43
ErhI Esp1396I FauNDI FriOI FspI GsuI HaeII Hin1I HincII HindII HindIII HpaI Hsp92I Ksp632I LspI MamI MfeI MflI MluI MluNI Mph1103I MroNI MscI MslI Msp17I MspA1I MunI Mva1269I NaeI NcoI NdeI NgoAIV NgoMI NheI NotI NsiI NspBII NspI NspV PaeR7I 6 1 1 6 1 7 4 7 3 3 2 1 7 7 1 2 1 12 1 1 1 2 1 8 7 5 1 3 2 3 1 2 2 2 1 1 5 1 1 1 360 757 840 1473 1989 2340 2430 234 620 1161 1506 1589 2161 3104 4095 837 887 1885 1966 2146 2509 4233 598 3020 3028 4966 122 175 258 444 2436 2484 3778 1186 2530 2822 1186 2530 2822 622 848 2822 122 175 258 444 2436 2484 3778 913 1135 1156 1246 1306 1510 3529 627 1214 2721 2809 585 609 1390 1604 1972 2604 3671 3688 4456 4468 4554 4565 2945 2076 5263 783 3070 2076 365 1073 1457 1991 2339 3547 3906 4065 122 175 258 444 2436 2484 3778 881 941 3682 4623 4868 2809 1854 2809 2908 785 3072 360 1989 2340 234 783 3070 783 3070 591 1736 2703 5263 881 941 3682 4623 4868 5210 627 613 c/cwwgg ccannnn/ntgg ca/tatg grgcy/c tgc/gca ctggag rgcgc/y gr/cgyc gty/rac gty/rac a/agctt gtt/aac gr/cgyc ctcttc tt/cgaa gatnn/nnatc c/aattg r/gatcy a/cgcgt tgg/cca atgca/t g/ccggc tgg/cca caynn/nnrtg gr/cgyc cmg/ckg c/aattg gaatgc gcc/ggc c/catgg ca/tatg g/ccggc g/ccggc g/ctagc gc/ggccgc atgca/t cmg/ckg rcatg/y tt/cgaa c/tcgag PflMI PinAI Ple19I PmaCI Pme55I PmlI Ppu10I PpuMI PshBI Psp124BI Psp1406I Psp5II PspEI PstNHI PvuI PvuII RcaI SacI SapI ScaI SexAI SfcI Sfr274I SfuI SnaBI SseBI SspBI SspI SstI StuI StyI Tth111I Van91I VneI VspI XbaI XcmI XhoI XhoII XmaIII XmnI Zsp2I 1 1 1 1 1 1 1 1 2 3 2 1 1 2 1 1 2 3 3 1 1 6 1 1 1 1 1 2 3 1 6 2 1 2 2 1 2 1 12 1 2 1 2430 1978 3949 1045 1945 1045 5259 1057 7 4143 620 1161 1506 3716 4089 1057 2635 591 1736 3949 941 3478 4486 620 1161 1506 913 1247 1510 3837 1404 1759 2545 2952 4072 4750 4941 613 627 340 1945 2691 3383 3513 620 1161 1506 1945 360 757 840 1473 1989 2340 860 1181 2430 3646 4892 7 4143 2713 1571 2677 613 585 609 1390 1604 1972 2604 3671 3688 4456 4468 4554 4565 2703 1705 3718 5263 ccannnn/ntgg a/ccggt cgat/cg cac/gtg agg/cct cac/gtg a/tgcat rg/gwccy at/taat gagct/c aa/cgtt rg/gwccy g/gtnacc g/ctagc cgat/cg cag/ctg t/catga gagct/c gctcttc agt/act a/ccwggt c/tryag c/tcgag tt/cgaa tac/gta agg/cct t/gtaca aat/att gagct/c agg/cct c/cwwgg gacn/nngtc ccannnn/ntgg g/tgcac at/taat t/ctaga ccannnnn/nnnntgg c/tcgag r/gatcy c/ggccg gaann/nnttc atgca/t

The following enzymes do not cut: Acc65I, AccIII, AclNI, AflII, AocI, ApaI, Asp718I, AvrII, BanIII, BbeI, BbuI, BclI, BfrI, BlnI, BlpI, Bpu1102I, Bsa29I, BscI, Bse21I, BseAI, BseCI, BsgI, BsiMI, BsiWI, BsmBI, Bsp106I, Bsp120I, Bsp13I, Bsp1720I, Bsp68I, BspDI, BspEI, BspTI, BspXI, Bst1107I, Bst98I, Bsu15I, Bsu36I, CelII, Cfr42I, Cfr9I, ClaI, CpoI, CspI, CvnI, Eco32I, Eco81I, EcoNI, EcoRV, EheI, Esp3I, FbaI, FseI, KasI, Kpn2I, KpnI, Ksp22I, KspI, MroI, MspCI, NarI, NruI, PacI, PaeI, Pfl23II, PmeI, PshAI, PspAI, PspALI, PspLI, PspOMI, PstI, RsrII, SacII, SalI, SbfI, SfiI, Sfr303I, SgfI, SgrAI, SmaI, SmiI, SpeI, SphI, SplI, SrfI, Sse8387I, SstII, SunI, SwaI, Vha464I, XmaI

Related products:
Current All ustious Fluorescent Protein Family Members: The founding member is mTFP1. mTFPG3 is a green FP with 3 amino difference from mTFP1. It has a slightly red-shifted emission spectrum and is 1.5 fold brighter compared to EGFP. While being very bright, mTFPG3 can be photobleached within ~5 sec, about 30 times faster than EGFP, suitable for certain cell-based assays that require a bright FP with very short half-life. mTFP0.7 is a precursor during the evolution of mTFP1. It has photo-switchable properties like Dronpa that cycles between fluorescent and nonfluorescent states. It may be developed into components in PALM/SIM applications.
ele

Basic Vectors Three vectors are available: pNCS-mWasabi, pmWasabi-N and pmWasabi-C. Subcellular Marker Vectors Twenty six vectors are available. Vectors in Viral Vectors All plasmid format vectors in Allele's Phoenix Retroviral vector or HiTiter Lentiviral Vectors.

IMPORTANT NOTICE TO PURCHASER: This product may be subjects of pending U.S. and foreign patents. Allele Biotech grants academic/government research institutions a worldwide, non-exclusive, royalty-free, limited license to use this product for non-commercial life science research use only. Such license specifically prohibits the right to sell or otherwise transfer this product or its derivatives to a third party.

Industry For-Profit Institutions: Institutions that wish to use this product are required to obtain a sub-licensing agreement from Allele Biotech. For more information please visit Fluorescent Protein Sublicense, or contact us via phone or email.

Allele Biotechnology and Pharmaceuticals 9924 Mesa Rim Road San Diego, CA 92121 Tel: (800) 991-RNAi (858) 587-6645 Fax: (858) 587-6692 [email protected] Copyright 2008-2010