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    Clustalw

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    bioenviron
    The document provides instructions for a bioinformatics lab experiment involving multiple sequence alignment and molecular phylogeny. The objective is to understand and perform multiple sequence alignment using CLUSTAL W and phylogenetic analysis using PHYLIP. The experiment involves logging into the EMBL/EBI website, selecting CLUSTAL Omega, choosing the molecule type, appending sequences, running the default parameters, and pasting the alignment results. Multiple sequence alignment is used to find evolutionary relationships and common ancestry between three or more biological sequences.

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    Clustalw

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    bioenviron
    215 views5 pages
    The document provides instructions for a bioinformatics lab experiment involving multiple sequence alignment and molecular phylogeny. The objective is to understand and perform multiple sequence alignment using CLUSTAL W and phylogenetic analysis using PHYLIP. The experiment involves logging into the EMBL/EBI website, selecting CLUSTAL Omega, choosing the molecule type, appending sequences, running the default parameters, and pasting the alignment results. Multiple sequence alignment is used to find evolutionary relationships and common ancestry between three or more biological sequences.

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    The document provides instructions for a bioinformatics lab experiment involving multiple sequence alignment and molecular phylogeny. The objective is to understand and perform multiple sequence alignment using CLUSTAL W and phylogenetic analysis using PHYLIP. The experiment involves logging into the EMBL/EBI website, selecting CLUSTAL Omega, choosing the molecule type, appending sequences, running the default parameters, and pasting the alignment results. Multiple sequence alignment is used to find evolutionary relationships and common ancestry between three or more biological sequences.

    Copyright:

    © All Rights Reserved
    Download as DOC, PDF, TXT or read online from Scribd
    Download as doc, pdf, or txt
    215 views5 pages

    Clustalw

    Uploaded by

    bioenviron
    The document provides instructions for a bioinformatics lab experiment involving multiple sequence alignment and molecular phylogeny. The objective is to understand and perform multiple sequence alignment using CLUSTAL W and phylogenetic analysis using PHYLIP. The experiment involves logging into the EMBL/EBI website, selecting CLUSTAL Omega, choosing the molecule type, appending sequences, running the default parameters, and pasting the alignment results. Multiple sequence alignment is used to find evolutionary relationships and common ancestry between three or more biological sequences.

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    LABORATORY MANUAL

    Exercise/Experiment Number:
    Lab Code / Lab
    : BTY412 Bioinformatics Lab
    Course / Branch
    : IV B.Tech. Biotechnology
    Title of the exercise/experiment : Multiple sequence alignment (CLUSTAL W) and
    Molecular phylogeny
    ______________________________________________________________

    STEP 1: INTRODUCTION
    OBJECTIVE OF THE EXERCISE/EXPERIMENT
    To understand and perform the multiple sequence alignment and
    phylogenetic analysis using CLUSTAL W and PHYLIP, respectively.

    STEP 2: ACQUISITION
    INTRODUCTION
    A multiple sequence alignment (MSA) is a sequence alignment of three or more
    biological sequences (protein, DNA, or RNA). From the query sequences we can find the
    evolutionary relationship by which they share a lineage and the descended from a common
    ancestor. Because three or more sequences of biologically relevant length can be difficult
    and are almost always time-consuming to align by hand, computational algorithms are
    used to produce and analyze the alignments. MSAs require more sophisticated
    methodologies than pairwise alignment because they are more computationally complex.
    Procedure:
    Step 1: Login to EMBL/ EBI website and select CLUSTAL Omega option under
    sequence analysis

    Step 2: Select the type of the molecule to be clustered

    Step 3: Select and append the sequences in the appropriate box

    Step 4: Run the default parameters and submit. Paste the screenshot of the
    alignment produced

    Result:
    The Clustal program was used to align related sequences

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