Valerie Griffin Aug 21 2014

RE: Ministry of Natural Resources Wildlife Scientific Collectors Authorization #1076879,
as approved for the Monarch Teacher Network of Canada.
To Valerie Griffin,
We are very pleased to have been approved for our Wildlife Scientific Collectors
Authorization by the Ontario Ministry of Natural Resources (MNR). Please read all of the
attached information carefully so that you are knowledgeable with all expectations and
commitments of this permit. To use this permit, you must abide by all expectations of this
permit. This permit is for the period between present and September 30, 2016.
Please note that this authorization:
Is not valid in provincial parks, park reserves, national parks, conservation areas,
crown game preserves or sanctuaries established under the Migratory Birds
Convention Act without written permission from the authorized person in charge of
the area concerned.
Does not allow access to any property without permission of the landowner.
Does not allow for propagation, buying or selling of species.
Covers only the species and numbers listed, which does not include any species at
risk in Ontario.
As part of this permit, we are required to report on your collecting, rearing and releasing
practices. Note that data reporting is important and that we are asking you to assist by
providing us with your data once per year. Data collection sheets for 2014-2016 are
provided (see attached sheets for each year of this permit and submission process).
Please note that you must have this permit with you at any time you are collecting, rearing
and/or releasing of the Monarch Butterfly species (Danaus Plexippus).
Our permit number with the MNR is #1076879, while your individual number as registered
with Toronto and Region Conservation (TRCA) is #14-07-2930-009. You are not entitled to
share this permit with any other individuals.
If you have any questions or concerns, please contact Vicky An, Coordinator - Education
Program Services at [email protected] or 289 268-3915.
Collection, Rearing and Release Data 2014 || Danaus Plexippus

Under the Authorization #1076879, we are required to record and submit all data related to the Monarch collection, rearing and release activities.
Please return all data to Vicky An at [email protected] for compilation under the Authorization #1076879, by November 2014. To reach Vicky An by telephone,
please call (289) 268-3915.
Date of collection
Location of collection site
(e.g. lot and concession,
address or GPS
coordinates)
Description of collection
site
Method of collection
Number and species of
individuals targeted
and collected
Disposition of
and collected (e.g. Died
in captivity, kept
temporarily and
released)
Name of collector

Please return all data to Vicky An at [email protected] for compilation under the Authorization #1076879, by November 2015. To reach Vicky An by telephone,
please call (289) 268-3915.
Date of collection
address or GPS
coordinates)
site
and collected
Disposition of
in captivity, kept
temporarily and
released)
Name of collector

Please return all data to Vicky An at [email protected] for compilation under the Authorization #1076879, by August 2016. To reach Vicky An by telephone, please
call (289) 268-3915.
Date of collection
address or GPS
coordinates)
site
and collected
Disposition of
in captivity, kept
temporarily and
released)
Name of collector
ANIMAL CARE & HANDLING PROTOCOL:

MONARCH TEACHER NETWORK OF CANADA/TRCA
Danaus plexippus Specimen Collection and Care (Standard)
HANDLING PROTOCOL
Handling of livestock at any stage should be minimized. If handling is necessary, the please follow the protocol below:
For all life stages, ensure that your hands are free from lotions, repellants, and/or soaps as these are often impactful
to the Monarch. Wash and dry your hands well.
EGG STAGE
Never touch the egg directly. Remove the leaf upon which it is sitting (from the Milkweed plant) and transport it to a
container where it can sit undisturbed until it hatches. Ensure that the stem of the leaf is wrapped in paper towel and
kept moist.
YOUNGEST INSTARS (first, second, and even third)

These instars are very fragile! Touching them directly should be avoided for the safety of the caterpillar. Ensure that
the instars always have fresh food milkweed. It is safest for the caterpillar to leave it on the leaf upon which it is
stationed, and set fresh leaves into the container. Attempting to move a motionless caterpillar by prodding may disturb
the molting process and injure the caterpillar it is best to allow it to move on its own. Remove frass from the container
daily.
OLDER INSTARS (third, fourth, and fifth instars)

These instars are hardier than the younger instars, but it is still best to minimize handling. It is not uncommon to see
these larger caterpillars on walk-a-bouts, which may demand their retrieval. The safest way to collect them is to allow
them to crawl onto a leaf or piece of paper, and then return them to the safety of their holding cage/container by
allowing them to crawl off of the paper or leaf onto a milkweed plant. Never try to pick them up by rolling them or
pinching them in your fingers.
PRE-PUPAE AND CHRYSALIS

When the caterpillar is going into the J, be careful not to touch, move, or disturb it.
If the chrysalis can remain safely where it has attached, leave it.
If it must be moved, create a slipknot in a piece of unflavoured dental floss, and slip it up to the point of attachment
(where the cremaster hooks into the silk). Gently tighten and secure the knot. Do not tie the knot lower on the
cremaster touching the silk is best, and should ensure that there is not damage to the forming adult.
ADULTS
When adults emerge they are very fragile! DO NOT TOUCH or try to move them, as they need time to expand their
wings fully, dry, and zip together the two pieces of the proboscis. It is best not to handle them at all on their first day
as an adult unless absolutely necessary.
When handling adults, (again, wait 24 hours after emergence) keep in mind that each contact will remove scales from
the Monarchs wings handling should be minimized. To safely transfer the adult from one cage to another, cupping
both hands around the adult is appropriate. For feeding the adult, you should use the four-pinched-wings method
gently pinching all four wings between the middle finger and thumb, and arch the first finger in front of the Monarch
upon which it can crawl. If the butterfly is not in a position to crawl onto a finger, gently ease it from its perch DO NOT
PULL, as you could cause injury to legs or clasps on the feet. Hold the Monarch so that its feet are in the puddle of sugar
water, which will prompt them to unfurl the proboscis and draw up the sugar water. Allow them to feed until they
retract the proboscis naturally, typically about two minutes.
If Monarchs are not being released immediately (should be released within 24 hours so that they can feed naturally),
you must be prepared to feed them twice per day, and provide water for puddling. Though fruits or flowering plants
may provide the nutrients they need and can be left in the holding cages, a sugar water mixture can be prepared (5 parts
water: 1 part sugar) for them.
ADDITIONAL NOTES:
Ensure that adults are released in an area safe from cars, roads, or activities that could cause injury while they are reorienting to the new environment.
Workshop
Classroom
Stages and maximum

quantity collected (wild)
Eggs (3-5), caterpillars (all
instars (15)), chrysalids (25), adults (2-15)
Eggs (2-3), caterpillars (all

instars (5)), chrysalids (12), adults (2-3)
*in most cases, teachers
collect just 2-3 eggs and
watch the complete
metamorphosis with these
specimens
Time in Captivity
1-2 weeks from
collection to workshop
(released on last day of
workshop, or as the
adults emerge)
2- 5 weeks
*released either as the
adults emerge, or
maintained through bidaily feeding for
tagging/group release
Care Protocol
Containment in rearing and holding cages, with appropriate
space, air movement.
Daily removal of frass.
Daily replacement of fresh milkweed.
Adults (bi-daily) feeding with appropriate sugar solution
and/or fruit/nectar sources.
Handling for training purposes only after proper instruction
on appropriate handling techniques.
Containment in rearing and holding cages, with appropriate
space, air movement.
Daily removal of frass.
Daily replacement of fresh milkweed.
Adults (bi-daily) feeding with appropriate sugar solution
and/or fruit/nectar sources (or immediate release.
Restricted handling primarily observation.

Valerie Griffin Aug 21 2014

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RE: Ministry of Natural Resources Wildlife Scientific Collectors Authorization #1076879,

as approved for the Monarch Teacher Network of Canada.

Collection, Rearing and Release Data 2014 || Danaus Plexippus

Collection, Rearing and Release Data 2015 || Danaus Plexippus

Collection, Rearing and Release Data 2016 || Danaus Plexippus

ANIMAL CARE & HANDLING PROTOCOL:

YOUNGEST INSTARS (first, second, and even third)

OLDER INSTARS (third, fourth, and fifth instars)

PRE-PUPAE AND CHRYSALIS

Stages and maximum

Eggs (2-3), caterpillars (all

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