Code No: RR212302 Set No.

1
II B.Tech I Semester Regular Examinations, November 2007
MICROBIOLOGY
(Bio-Technology)
Time: 3 hours Max Marks: 80
Answer any FIVE Questions
All Questions carry equal marks
⋆⋆⋆⋆⋆

1. Describe the similarities between Germ theory of disease and Germ theory of fer-
mentation. [8+8]

2. Describe different Immunological and biochemical tests used for identification of

bacteria. [16]

3. Describe the general characters of Protozoa with special reference to pathogenic

forms. [16]

4. How is energy supplied to drive a conc. gradient across the membrane of the cell?
[16]

5. Explain different methods of measuring microbial growth. Write the advantages

and disadvantages of each method. [16]

6. Define water activity. Explain how water activity influences the growth of different
microorganisms? [16]

7. Discuss about the factors that influence the effectiveness of Antimicrobial chemical
agents. [16]

8. What is a Standard operating procedure? Develop an SOP for autoclaving. [16]

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Code No: RR212302 Set No. 2
II B.Tech I Semester Regular Examinations, November 2007
MICROBIOLOGY
(Bio-Technology)
Time: 3 hours Max Marks: 80
Answer any FIVE Questions
All Questions carry equal marks
⋆⋆⋆⋆⋆

1. Justify the statement. “Microorganisms are more than just agents of disease”.
[16]

2. Define a “Bacterial species”. Explain the difficulties encountered in classification

of bacteria. [16]

3. Describe the economic and ecological importance of Gram negative Aerobic Rods
and Cocci. [8+8]

4. How is energy supplied to drive a conc. gradient across the membrane of the cell?
[16]

5. Classify bacteriological media based on their utility and explain about them with
suitable examples. [16]

6. Define water activity. Explain how water activity influences the growth of different
microorganisms? [16]

7. Describe Filtration as a method of sterilization and its practical application in a

microbiology laboratory and in every day life. [16]

8. Give an account of monitoring air quality. Mention methods for providing clean
air rooms and chambers. [16]

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Code No: RR212302 Set No. 3
II B.Tech I Semester Regular Examinations, November 2007
MICROBIOLOGY
(Bio-Technology)
Time: 3 hours Max Marks: 80
Answer any FIVE Questions
All Questions carry equal marks
⋆⋆⋆⋆⋆

1. Describe how the microbial world was discovered and significance of this discovery.
[8+8]

2. Describe Five-kingdom system of Whittaker’s classification of bacteria. [16]

3. Describe asexual reproduction in fungi with suitable examples. [16]

4. Discuss about different transport mechanisms in operation in bacteria. [16]

5. Explain different methods of measuring microbial growth. Write the advantages

and disadvantages of each method. [16]

6. Define water activity. Explain how water activity influences the growth of different
microorganisms? [16]

7. Explain why a single chemical may not be an ideal disinfectant. [16]

8. What is a Standard operating procedure? Develop an SOP for autoclaving. [16]

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Code No: RR212302 Set No. 4
II B.Tech I Semester Regular Examinations, November 2007
MICROBIOLOGY
(Bio-Technology)
Time: 3 hours Max Marks: 80
Answer any FIVE Questions
All Questions carry equal marks
⋆⋆⋆⋆⋆

1. Why is the period 1880-1900 considered as the Golden era for microbiology? [16]

2. What are “Signature sequences” and Polymerase chain reaction? How are they
useful in classification of bacteria? [16]

3. (a) Discuss the characteristics of algae that are used as a basis for algal classifi-
cation.
(b) What are the attributes Euglena shares with plants and animals? [8+8]

4. How is energy supplied to drive a conc. gradient across the membrane of the cell?
[16]

5. Differentiate between enrichment medium and enriched medium. Write on the

enrichment culturing and its importance. [16]

6. Define water activity. Explain how water activity influences the growth of different
microorganisms? [16]

7. Describe the Phenol coefficient method used for evaluation of disinfectants of phenol
type. [16]

8. Write briefly on

(a) QC of foods
(b) Sanitization [8+8]

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