Hplc ASSAY

Simple and rapid assay method is primary prerequisite in quality assurance department for any pharmaceutical dosage form. When two drugs are combined in a single dosage form, it becomes difficult to estimate them simultaneously with required accuracy and precision. Here we have proposed a simple and rapid UV spectrophotometric assay method for simultaneous determination of amlodipine besylate and valsartan in both bulk drug and commercial dosage forms. Method employed was Zero absorbance method where amlodipine and valsartan were determined at 363 nm and 290 nm respectively. At 363 nm valsartan don't have absorption and at 290 nm amlodipine has zero absorption. Linearity was established at 8 µg/mL -80 µg/mL for amlodipine (y= 0.012X + 0.002 R 2 = 0.999) and 4 µg/mL -300 µg/ml for Vasartan (y= 0.002X + 0.017 R 2 = 0.999). Method was found to be accurate and precise with %RSD<2 for all the validation parameters employed. Method was found to be very sensitive with LOD 2.4 µg/mL and 3.6 µg/mL whereas LOQ was 7.5 µg/mL and 11 µg/mL for Amlodipine besylate and Valsartan respectively. As method uses methanol as a solvent to dissolve, it is cost effective for routine analysis in quality assurance department for determination of these drugs individually or simultaneously.

Adhatoda vasica (L.) Nees (Acanthaceae) is a well known plant drug in Ayurvedic medicine, used for the treatment of different types of diseases and disorders. The tissue cultures of this plant species were established on Murashige and Skoog culture medium by using various concentrations of growth hormones (0.5-5.0 mg/L α-napththaleneacetic acid + 0.5-3.5 mg/L 6-benzylaminopurine). The concentration of vasicine acetate was found higher (0.51  0.519%) in six week old callus than in vivo (0.47  0.556%) plant material (roots). The antioxidant activity was evaluated by using DPPH-radical scavenging, reducing power and superoxide anion scavenging models. We found that 2-acetyl benzyl amine showed maximum antioxidant activity against various antioxidant models, including DPPH-radical scavenging (40.59 ± 0.774%), reducing power (461.55 ± 0.402%) and superoxide anion scavenging (52.54 ± 0.553%) than other isolated alkaloids at 10-30 μM concentrations. Our observed results suggest that the pyrroloquinazoline alkaloids of A. vasica demonstrated strong antioxidant activity, which could be used as potent drugs against different types of oxidative stresses.