Esox lucius
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Most cited papers in Esox lucius
In this study we discriminate three freshwater fish species (the shad Alosa agone Scopoli 1786, the whitefish Coregonus macrophthalmus Nüsslin1882 and the roach Rutilus rutilus Linnaeus 1758) by Matrix-Assisted Laser... more
In this study we discriminate three freshwater fish species (the shad Alosa agone Scopoli 1786, the whitefish Coregonus macrophthalmus Nüsslin1882 and the roach Rutilus rutilus Linnaeus 1758) by Matrix-Assisted Laser Desorption/Ionization- Time Of Flight Mass Spectrometry (MALDI-TOF MS) using both muscle and liver tissues. The technology enables to analyze tissues after a simple single-step extraction procedure without any further purification. The molecular profile of muscle tissues showed the most intense peaks at m/z range of 11,354.0 (±2.0 SD) Da, 3508.5 (±1.5 SD) Da and 8567.2 (±1.1 SD) Da for the shad, the whitefish and the roach respectively.
The molecular profiles of liver tissues exhibit most of the highest peak intensities in the range between 2000 and 6000 m/z values.
The roach shows the clearest pattern with high intensities detected at mass ranges between 3000 and 3550 Da with maxima at m/z 3035.2 (±0.2) Da and 3468.7 (±0.3) Da. The shad shows a shared high peak at m/z 3429.0 (±0.3) Da. The whitefish shows a group of major peaks in the m/z range of 3000-3700 Da with the highest being at 3635 (±0.3) Da. The overall signal pattern generated is highly specific for each species and, according to cluster analyses based on the total number of peaks, we could discriminate the three species.
The molecular profiles of liver tissues exhibit most of the highest peak intensities in the range between 2000 and 6000 m/z values.
The roach shows the clearest pattern with high intensities detected at mass ranges between 3000 and 3550 Da with maxima at m/z 3035.2 (±0.2) Da and 3468.7 (±0.3) Da. The shad shows a shared high peak at m/z 3429.0 (±0.3) Da. The whitefish shows a group of major peaks in the m/z range of 3000-3700 Da with the highest being at 3635 (±0.3) Da. The overall signal pattern generated is highly specific for each species and, according to cluster analyses based on the total number of peaks, we could discriminate the three species.
- by pietro volta and +1
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- Proteomics, Fish Biology, Protected areas, Habitat complexity
In this research, Pike (Esox lusius) one of the most valuable commercial marine species has been evaluated for genetic structure in Anzali wetland using microsatellite markers. 60 specimens of adult pikes were sampled from two spawning... more
In this research, Pike (Esox lusius) one of the most valuable commercial marine species has been evaluated for genetic structure in Anzali wetland using microsatellite markers. 60 specimens of adult pikes were sampled from two spawning seasons, winter and spring in Anzali wetland. Five pairs of microsatellites, tested on the genomic deoxyribonucleic acid (DNA). All loci of microsatellite produced polymorphic bands as polymorphic loci were used to analyze the genetic variation of the pike. Analyses revealed that average of alleles per locus were 10.8 (range 9 to 13 alleles). All sampled seasons contained private alleles. The average observed and expected heterozygosity was 0.914 and 0.885, respectively. The inbreeding coefficient values of five microsatellite loci were negative. With the exception of a locus in spring, all loci significantly deviated from H-W equilibrium (P<0.01). Based on AMOVA, RST and FST values were significant between seasons (P<0.01). The genetic distance between populations was 0.442, which indicates that the genetic difference among the studied populations is significant. These results support the existence of different genetic populations in spawning seasons in this area.