As one of the ex situ conservation sites, Purwodadi Botanic Garden (PBG) has Intsia bijuga as its... more As one of the ex situ conservation sites, Purwodadi Botanic Garden (PBG) has Intsia bijuga as its collection with high economic value for its high quality wood. It is categorised as Near Threatened in the IUCN Red List. Its efficacy as herb is due to the presence of its various chemical compounds. The purpose of this study was to characterize the morphology of I. bijuga cultivated in PBG and to reveal its phytochemical compounds, as well as their health benefits. This research was conducted at the PBG in April–May 2020. The plant material was obtained from PBG collection. The plant morphology was characterized by direct observation in the field, while information regarding phytochemical compounds of I. bijuga along with their benefits was obtained by literature review. The data obtained was analyzed descriptively. The results showed that I. bijuga collected by PBG came from Maluku and Java. Both have morphological characteristics that are not very different, i.e., they are trees, th...
Kepel is a common name for Stelochocarpus burahol, a plant that spreads naturally in Indonesia. I... more Kepel is a common name for Stelochocarpus burahol, a plant that spreads naturally in Indonesia. It is a member of Annonaceae with fruit the size of a hand or kepel (Javanese) hence it is called kepel. The plant commonly found in the Special Region of Yogyakarta (a territory ruled by the Sultan), and is designated as a Flora of Provincial Identity. In ancient times kepel was considered as a plant of the royal household. Besides its small flesh the fruit was economically less prospective thus not too attractive to locals. Therefore its current population is quite limited and has begun to be rarely found. Recent studies have shown that the species has potential as medicinal plants for its antioxidant, antifungal, and antiseptic properties. This added value is expected to attract people’s interest to put investment on the plant’s potential future use as well as improving its conservation status. Kepel is a plant that spre...
Pencemaran tembaga di daerah Rungkut-Surabaya telah melebihi ambang batas sehingga dapat menganca... more Pencemaran tembaga di daerah Rungkut-Surabaya telah melebihi ambang batas sehingga dapat mengancam kehidupan organisme di laut. Tembaga tidak dapat didegradasi dan beracun pada konsentrasi tinggi sehingga diperlukan pengolahan limbah yang dapat menurunkan konsentrasi tembaga di lingkungan. Khamir resisten tembaga dapat diisolasi dari daerah tercemar dan dapat dijadikan sebagai agen bioremediasi untuk mengatasi pencemaran tembaga. Penelitian ini bertujuan untuk melakukan isolasi dan uji resistensi khamir resisten tembaga dari limbah industri. Sampel diambil dari lumpur aktif pusat pengolahan limbah PT. SIER Surabaya. Isolasi dilakukan dengan pengenceran sampel limbah kemudian diinokulasikan dengan metode sebar ke dalam medium Yeast Extract Peptone Dextrose Agar yang diperkaya dengan CuSO 4. Uji resistensi dilakukan dengan menginokulasikan isolat murni dengan metode gores ke dalam medium Agar yang mengandung berbagai konsentrasi CuSO 4 untuk menentukan nilai Minimum Inhibitory Concentration (MIC). Hasil isolasi diperoleh delapan isolat khamir dengan kode ES6.1, ES8.1, ES9.2, ES9.3, ES9.4, ES9.5, ES10.2, dan ES10.4. Nilai MIC isolat khamir tersebut berkisar antara 16-20 Mm CuSO 4. Isolat yang paling resisten adalah isolat dengan kode ES9.3 dengan nilai MIC= 20 mM CuSO 4 , sehingga isolate ini dipilih sebagai isolate yang paling resisten terhadap polutan di Wilayah Rungkut,
AbstrakSARS-CoV-2 dikenal sebagai agen penyebab COVID-19. Penyakit ini pertama kali muncul di Cin... more AbstrakSARS-CoV-2 dikenal sebagai agen penyebab COVID-19. Penyakit ini pertama kali muncul di Cina yang dalam beberapa bulan telah menyebar secara masif ke 198 negara lain termasuk Indonesia, dan menyebabkan pandemik global. Sekalipun vaksin telah ditemukan namun laju mutasi virus yang sangat cepat menjadikannya kurang efektif. Beberapa dekade terakhir, para ilmuwan telah mengidentifikasi senyawa-senyawa tumbuhan yang mempunyai potensi sebagai aktivitas anti-coronavirus. Tujuan penelitian ini adalah untuk mengkaji senyawa aktif yang dapat menghambat aktivitas coronavirus dan menentukan koleksi tumbuhan Kebun Raya Purwodadi yang mengandung senyawa tersebut. Penelitian dilakukan melalui review dan kompilasi berbagai literatur terkait. Hasil penelitian menunjukkan bahwa KR Purwodadi memiliki 49 jenis, 38 marga, dan 24 suku koleksi tumbuhan yang diprediksi mengandung 12 jenis senyawa anti-coronavirus, yaitu amentoflavone, beta-sitosterol, betulinic acid, curcumin, emodin, epigallocatech...
Background: CoVID-19, caused by a new type of coronavirus named SARS-CoV-2, has become a pandemic... more Background: CoVID-19, caused by a new type of coronavirus named SARS-CoV-2, has become a pandemic. Together with SARS-CoV and MERS-CoV, CoVID-19 is a large global outbreak of coronavirus infection; however, its rate of spread is much higher. Since the vaccines and anti-SARS-CoV-2 have not been found, a faster control mechanism is much needed. Traditional herbs have shown the potential for this purpose, as has been demonstrated by the Chinese Government with a high success rate. One of the herbs used was Lindera aggregata, which is part of the collection in Purwodadi Botanic Gardens. Objectives: Through in silico study, this research aims to reveal the secondary metabolites contained in L. aggregata that have the potential to serve as anti-SARS-CoV-2 medication as well as showcase their inhibitory mechanisms. The research was conducted through molecular docking analysis of terpenoids and alkaloids contained in the root of L. aggregata, with target proteins 3CLpro, PLpro, Spike, and ACE 2 playing a role in SARS-CoV-2 infection. Result: All analyzed compounds tended to interact with all four target proteins with different binding affinity values, but the interaction seemed stronger with 3CLpro and Spike. Terpenoids, linderane and linderalactone had the strongest interaction tendency with 3CLpro, PLpro, and Spike; the compound norboldine, an alkaloid, had the strongest interaction with ACE 2, with a binding affinity value of -8.2 kcal/mol. Terpenoids and alkaloids contained in the root of L. aggregata, which caused inhibition of adsorption and replication of SARS-CoV-2, could serve as anti-SARS-CoV-2.
Journal of Tropical Biodiversity and Biotechnology, 2022
Wild bananas provide important genetic materials for further banana improvement, therefore they n... more Wild bananas provide important genetic materials for further banana improvement, therefore they need to be conserved and studied. This study aimed to describe morphological characteristics of plant and seed and also to study the seed germination of wild banana M. acuminata var. flava (Ridl.) Nasution. The morphological characteristics were observed descriptively by referring to the descriptor for banana. The internal and external morphology of the seeds were observed using a digital microscope. The germination testing was carried out by a completely randomized design, using fresh seeds extracted from a bunch of fruits with two ripeness levels i.e. fully-ripe (yellow peel) and under-ripe (green-yellow peel). The data resulted was then analyzed using an independent t-test. The results showed that M. acuminata var. flava is characterized as a perennial herb; pseudostem height ≥3 m; male bud like a top with prominent green-yellow bracts; fruit curved and tasted mild-sweet when ripe. The...
Pomegranate (Punica granatum L.) is a shrub with a height of up to 5 meters belonging to Lythrace... more Pomegranate (Punica granatum L.) is a shrub with a height of up to 5 meters belonging to Lythraceae. It came from Iran and is now spread throughout the world. The use of this plant for traditional medicine has long been known, including by people in Indonesia. Purwodadi Botanical Gardens as one of the ex-situ conservation areas also collects this plant. This writing aims to determine the existence of pomegranate as a collection of the Purwodadi Botanical Gardens, explore the use of pomegranate in traditional medicine in Indonesia, and describe scientific experimental research that supports the potential of pomegranate as a medicine. The data were obtained through a literature study by browsing the collections catalog of the Purwodadi Botanical Gardens, while information on the use of pomegranate as an ingredient in traditional medicine and scientific experimental research on the medical benefits of pomegranate was obtained through relevant journals. Purwodadi Botanical Gardens has four collection numbers of pomegranate, namely XIV.G.I. and XXII.A.I. Pomegranate has long been used in traditional Indonesian medicine, especially in the areas of Madura, Luwu Timur, Baubau, and Bima. Some of the diseases that can be treated with pomegranate include canker sores, fever, intestinal worms infection, hypertension, and cough; and the parts of the plant often used are the fruit and seeds. Scientific studies have proven that pomegranate has oral antibacterial activity so that it can maintain oral health and has potential as an anticancer drug. This information is expected to increase the value of the benefits of pomegranate and encourage the community to participate in conserving it through cultivation efforts.
Perbanyakan vegetatif Hoya melalui stek daun pada tiga macam media, yaitu tanah, pasir, dan cocop... more Perbanyakan vegetatif Hoya melalui stek daun pada tiga macam media, yaitu tanah, pasir, dan cocopeat. Parameter yang diamati meliputi persentase tumbuhnya akar dan jumlah akar
Penyakit-penyakit neurodegeneratif telah menjangkiti jutaan orang di seluruh dunia dengan angka k... more Penyakit-penyakit neurodegeneratif telah menjangkiti jutaan orang di seluruh dunia dengan angka kejadian yang semakin meningkat. Hingga saat ini belum ada pendekatan terapeutik untuk menghentikan perkembangan penyakit-penyakit neurodegeneratif tersebut. Di sisi lain, produk-produk alami, terutama yang berasal dari tumbuhan, memiliki senyawa-senyawa dengan aktivitas anti-neurodegeneratif. Kebun Raya Purwodadi (KRP), sebagai salah satu lembaga konservasi tumbuhan ex situ , diduga menyimpan kekayaan plasma nutfah yang berpotensi sebagai agen neuroproteksi. Penulisan ini bertujuan untuk menginventarisasi koleksi KRP yang berpotensi sebagai agen neuroproteksi melalui studi literatur dan penelusuran katalog KRP. Hasil inventarisasi menunjukkan bahwa sebanyak 53 jenis koleksi KRP dari 21 suku berpotensi sebagai agen neuroproteksi. Lima di antaranya memiliki senyawa aktif lebih dari satu, yaitu Intsia bijuga ( resveratrol & dihydromyricetin ), Syzygium cumini ( kaempferol & dihydromyricetin...
Background: CoVID-19, caused by a new type of coronavirus named SARS-CoV-2, has become a pandemic... more Background: CoVID-19, caused by a new type of coronavirus named SARS-CoV-2, has become a pandemic. Together with SARS-CoV and MERS-CoV, CoVID-19 is a large global outbreak of coronavirus infection; however, its rate of spread is much higher. Since the vaccines and anti-SARS-CoV-2 have not been found, a faster control mechanism is much needed. Traditional herbs have shown the potential for this purpose, as has been demonstrated by the Chinese Government with a high success rate. One of the herbs used was Lindera aggregata, which is part of the collection in Purwodadi Botanic Gardens. Objectives: Through in silico study, this research aims to reveal the secondary metabolites contained in L. aggregata that have the potential to serve as anti-SARS-CoV-2 medication as well as showcase their inhibitory mechanisms. Methods: The research was conducted through molecular docking analysis of terpenoids and alkaloids contained in the root of L. aggregata, with target proteins 3CLpro, PLpro, Spi...
Mangrove forest is located along the coastal area and regularly waterlogged because it is influen... more Mangrove forest is located along the coastal area and regularly waterlogged because it is influenced by the tides, and has an important ecological function for life. Mangrove forest, especially in urban areas, is the most threatened and damaged ecosystem. Surabaya has a mangrove forest in the Mangrove Botanic Garden of Gunung Anyar (MBGGA) with an area of 32,761 m2 and has the potential for educational tourism. Scientific information concerning plant species in MBGGA is needed to support this potential. This study aimed to inventory the mangrove plants and their associated species in MBGGA and their potential as medicinal plants. The inventory was carried out by tracking method while information on medicinal potential was obtained through literature study. The results showed that MBGGA had 14 mangrove species and 11 mangrove-associated species. Around 18 species are included in the least concern category and one in the vulnerable category of the IUCN red list. All plant species in M...
The effect of gibberellin at multiplication stage on somatic embryo growth and maturation and pla... more The effect of gibberellin at multiplication stage on somatic embryo growth and maturation and plantlet regeneration of tangerine (Citrus reticulata Blanco.) var. Batu 55 was assessed. Somatic embryo at globular phase was cultured on MT media + 30 gL-1 sucrose and various concentrations of gibberellin (0, 2, 4, 6, and 8 ppm). The somatic embryo was maturated on MT media + 500 ppm malt extract + 73 mM sorbitol + 73 mM galactose. Cotyledonary somatic embryo was regenerated into plantlet on MT media + 500 ppm malt extract + 30 gL-1 sucrose + 2 ppm GA 3. The results showed that the addition of gibberellin in somatic embryo multiplication stage increased somatic embryo growth and maturation and plantlet regeneration of tangerine. Optimum concentration of gibberellin needed for somatic embryo growth was 4 ppm which yielded twofold fresh weight compared to control. The percentage of maturation was very low below 5%. The addition of gibberellin in media at embryo multiplication stage slightly increased the percentage of maturation about 1-2%. Thirty percent of cotyledonary embryo was able to regenerate into plantlet. The addition of gibberellin in media at embryo multiplication stage increased the regeneration percentage, even the addition of 8 ppm gibberelline yielded regeneration percentage up to 70%.
Physalis angulata L. (Ciplukan) is one member of Solanaceae that has a potential as herbal medici... more Physalis angulata L. (Ciplukan) is one member of Solanaceae that has a potential as herbal medicine. This plant grows wild in the crop fields, forest edges, etc. However, ciplukan is increasingly difficult to find recently. In vitro callus is an alternative source to produce secondary metabolite production as well as to regenerate plants through indirect organogenesis. This study aims to identify the response of hypocotyl explants on in vitro callus formation induced by a combination of auxin and cytokinins. Two types of cytokinins, Kinetin and BAP (0.5 ppm) were combined with three types of auxin, i.e. 2.4-D, IBA and IAA, at three concentrations 0.5, 1.0 and 1.5 ppm. In all combinations of cytokinin and auxin, 50-100% of hypocotyl explants derived from in vitro seedling were able to produce callus either in a compact or watery friable texture. In MS medium supplemented with 2.4-D, callus FW (fresh weight) began to decline in the fourth week after culture. Callus FW that increased until 5 weeks of culture was obtained in medium IAA 0.5 + Kin 0.5, IBA 1.0 + Kin 0.5 and IBA 1 + BA 0.5. Almost all calli induced on a medium + Kinetin also produced roots. While medium + BAP was able to induce shoots regeneration.
Tumbuhan telah dikenal sebagai sumber obat alami yang dapat menyembuhkan berbagai macam penyakit ... more Tumbuhan telah dikenal sebagai sumber obat alami yang dapat menyembuhkan berbagai macam penyakit karena mengandung senyawa metabolit sekunder dengan aktivitas biomedik. Tumbuhan paku merupakan kelompok tumbuhan tertua di bumi yang memiliki lebih dari 12.000 jenis. Tumbuhan paku mengandung senyawa metabolit sekunder yang berpotensi obat berdasarkanstudi etnobotani dan farmakologi. Sebanyak 20 suku dan 38 marga tumbuhan paku dikoleksi dan dikonservasi secara ex situ oleh Kebun Raya (KR) Purwodadi. Nephrolepis merupakan salah satu marga dari koleksi tumbuhan paku KR Purwodadi dengan jumlah spesimen paling banyak. Nephrolepis termasuk kelompok tumbuhan paku epifit atau setengah epifit yang daunnya tersusunmenyirip tunggal seperti pedang. Selama ini Nephrolepis hanya dikenal sebagai bahan sayuran dan tanaman hias oleh masyarakat. Namun belum ada informasi mengenai potensi obat dari koleksi Nephrolepis tersebut. Oleh karena itu, penelitian ini dilakukan untuk menginventarisasi dan mengara...
BromoTenggerSemeruNationalPark(BTSNP)isoneofnaturalconservationareasinEastJavawitharound1,025plan... more BromoTenggerSemeruNationalPark(BTSNP)isoneofnaturalconservationareasinEastJavawitharound1,025plantspecies.Impatiensplatypetala,locallyknownaspacartere,isaplantspeciesmostlybefoundinmountainousareasofJavaandwithhighpotentialasmedicinalandornamentalplant.However,researchonthisspeciesisstilllimited.Thisstudywasconductedtoinventory,describethemorphologicalcharacteristicsandtesttheseedviabilityofpacartereinfourresortsofBTSNP,namelyRPTNGunungPenanjakan,CobanTrisula,Senduro,andRanuDarungan.Surveymethodwasusedtoinventorytheplantpopulations.Directfieldobservationwasusedtocharacterizetheplantmorphology.Seedsviabilitytestwasconductedthroughgerminationonstrawpapermedia.TheresultsshowedthatpacarterepopulationswerefoundinthosefourBTSNPresorts,onshadeandhumidhabitats,alsoinopenareaswithwetsoilconditions.Theplantischaracterizedasanerectparennialherb,spursedpinkflower,capsulafruitwhichdehiscentwhenripe,withflattenedcone-shapedandsmoothhairyseeds.Seedgerminationtestshowedthattheseedsbegantogerminateo...
Biosaintifika: Journal of Biology & Biology Education
Bauhinia winitii Craib (Fabaceae, Caesalpinioideae) is a woody climber which is currently include... more Bauhinia winitii Craib (Fabaceae, Caesalpinioideae) is a woody climber which is currently included in endangered species list. B. winitii seeds are orthodox seeds in which it has hard coat morphologically. Therefore, B. winitii seeds often undergo physical dormancy which can be broken through immersion in certain liquid media. This study aimed to characterize the morphology of B. winitii seeds and determine the pre-sowing treatment method to accelerate the seeds germination of B. winitii. External morphological characterization was carried out by observing the seeds quantitative and qualitative parameters. Characterization of seeds internal morphology was conducted using a digital microscope. Physical dormancy breaking was carried out by immersion in warm and cold water for 24 hours. Morphological characterization data were analyzed descriptively, while seed germination data were analyzed quantitatively by using one-way ANOVA followed by LSD test (with confidence level of 95%). B. ...
Purwodadi Botanic Garden (KR Purwodadi) is an ex situ conservation institution for dry climate lo... more Purwodadi Botanic Garden (KR Purwodadi) is an ex situ conservation institution for dry climate lowland plants aiming at saving and protecting biodiversity from extinction. In supporting ex situ conservation efforts, plant exploration activities in their natural habitat need to be carried out. Alas Purwo National Park has a lowland forest area with abundant plant diversity. In addition to functioning as a conservation forest, this area is also a natural and religious tourist attraction, so it is likely to undergo ecosystem damage. Therefore, this plant diversity study with the activity of plants exploration and collection was carried out for the purpose of ex situ conservation in KR Purwodadi. This study was conducted in August to September 2019 using an explorative method. A total of 24 species were obtained, of which 8 species were not yet cultivated by KR Purwodadi. Besides, there were 5 species included in the IUCN red list, namely Aglaonema simplex, Brucea javanica, Cycas rumphi...
As one of the ex situ conservation sites, Purwodadi Botanic Garden (PBG) has Intsia bijuga as its... more As one of the ex situ conservation sites, Purwodadi Botanic Garden (PBG) has Intsia bijuga as its collection with high economic value for its high quality wood. It is categorised as Near Threatened in the IUCN Red List. Its efficacy as herb is due to the presence of its various chemical compounds. The purpose of this study was to characterize the morphology of I. bijuga cultivated in PBG and to reveal its phytochemical compounds, as well as their health benefits. This research was conducted at the PBG in April–May 2020. The plant material was obtained from PBG collection. The plant morphology was characterized by direct observation in the field, while information regarding phytochemical compounds of I. bijuga along with their benefits was obtained by literature review. The data obtained was analyzed descriptively. The results showed that I. bijuga collected by PBG came from Maluku and Java. Both have morphological characteristics that are not very different, i.e., they are trees, th...
Kepel is a common name for Stelochocarpus burahol, a plant that spreads naturally in Indonesia. I... more Kepel is a common name for Stelochocarpus burahol, a plant that spreads naturally in Indonesia. It is a member of Annonaceae with fruit the size of a hand or kepel (Javanese) hence it is called kepel. The plant commonly found in the Special Region of Yogyakarta (a territory ruled by the Sultan), and is designated as a Flora of Provincial Identity. In ancient times kepel was considered as a plant of the royal household. Besides its small flesh the fruit was economically less prospective thus not too attractive to locals. Therefore its current population is quite limited and has begun to be rarely found. Recent studies have shown that the species has potential as medicinal plants for its antioxidant, antifungal, and antiseptic properties. This added value is expected to attract people’s interest to put investment on the plant’s potential future use as well as improving its conservation status. Kepel is a plant that spre...
Pencemaran tembaga di daerah Rungkut-Surabaya telah melebihi ambang batas sehingga dapat menganca... more Pencemaran tembaga di daerah Rungkut-Surabaya telah melebihi ambang batas sehingga dapat mengancam kehidupan organisme di laut. Tembaga tidak dapat didegradasi dan beracun pada konsentrasi tinggi sehingga diperlukan pengolahan limbah yang dapat menurunkan konsentrasi tembaga di lingkungan. Khamir resisten tembaga dapat diisolasi dari daerah tercemar dan dapat dijadikan sebagai agen bioremediasi untuk mengatasi pencemaran tembaga. Penelitian ini bertujuan untuk melakukan isolasi dan uji resistensi khamir resisten tembaga dari limbah industri. Sampel diambil dari lumpur aktif pusat pengolahan limbah PT. SIER Surabaya. Isolasi dilakukan dengan pengenceran sampel limbah kemudian diinokulasikan dengan metode sebar ke dalam medium Yeast Extract Peptone Dextrose Agar yang diperkaya dengan CuSO 4. Uji resistensi dilakukan dengan menginokulasikan isolat murni dengan metode gores ke dalam medium Agar yang mengandung berbagai konsentrasi CuSO 4 untuk menentukan nilai Minimum Inhibitory Concentration (MIC). Hasil isolasi diperoleh delapan isolat khamir dengan kode ES6.1, ES8.1, ES9.2, ES9.3, ES9.4, ES9.5, ES10.2, dan ES10.4. Nilai MIC isolat khamir tersebut berkisar antara 16-20 Mm CuSO 4. Isolat yang paling resisten adalah isolat dengan kode ES9.3 dengan nilai MIC= 20 mM CuSO 4 , sehingga isolate ini dipilih sebagai isolate yang paling resisten terhadap polutan di Wilayah Rungkut,
AbstrakSARS-CoV-2 dikenal sebagai agen penyebab COVID-19. Penyakit ini pertama kali muncul di Cin... more AbstrakSARS-CoV-2 dikenal sebagai agen penyebab COVID-19. Penyakit ini pertama kali muncul di Cina yang dalam beberapa bulan telah menyebar secara masif ke 198 negara lain termasuk Indonesia, dan menyebabkan pandemik global. Sekalipun vaksin telah ditemukan namun laju mutasi virus yang sangat cepat menjadikannya kurang efektif. Beberapa dekade terakhir, para ilmuwan telah mengidentifikasi senyawa-senyawa tumbuhan yang mempunyai potensi sebagai aktivitas anti-coronavirus. Tujuan penelitian ini adalah untuk mengkaji senyawa aktif yang dapat menghambat aktivitas coronavirus dan menentukan koleksi tumbuhan Kebun Raya Purwodadi yang mengandung senyawa tersebut. Penelitian dilakukan melalui review dan kompilasi berbagai literatur terkait. Hasil penelitian menunjukkan bahwa KR Purwodadi memiliki 49 jenis, 38 marga, dan 24 suku koleksi tumbuhan yang diprediksi mengandung 12 jenis senyawa anti-coronavirus, yaitu amentoflavone, beta-sitosterol, betulinic acid, curcumin, emodin, epigallocatech...
Background: CoVID-19, caused by a new type of coronavirus named SARS-CoV-2, has become a pandemic... more Background: CoVID-19, caused by a new type of coronavirus named SARS-CoV-2, has become a pandemic. Together with SARS-CoV and MERS-CoV, CoVID-19 is a large global outbreak of coronavirus infection; however, its rate of spread is much higher. Since the vaccines and anti-SARS-CoV-2 have not been found, a faster control mechanism is much needed. Traditional herbs have shown the potential for this purpose, as has been demonstrated by the Chinese Government with a high success rate. One of the herbs used was Lindera aggregata, which is part of the collection in Purwodadi Botanic Gardens. Objectives: Through in silico study, this research aims to reveal the secondary metabolites contained in L. aggregata that have the potential to serve as anti-SARS-CoV-2 medication as well as showcase their inhibitory mechanisms. The research was conducted through molecular docking analysis of terpenoids and alkaloids contained in the root of L. aggregata, with target proteins 3CLpro, PLpro, Spike, and ACE 2 playing a role in SARS-CoV-2 infection. Result: All analyzed compounds tended to interact with all four target proteins with different binding affinity values, but the interaction seemed stronger with 3CLpro and Spike. Terpenoids, linderane and linderalactone had the strongest interaction tendency with 3CLpro, PLpro, and Spike; the compound norboldine, an alkaloid, had the strongest interaction with ACE 2, with a binding affinity value of -8.2 kcal/mol. Terpenoids and alkaloids contained in the root of L. aggregata, which caused inhibition of adsorption and replication of SARS-CoV-2, could serve as anti-SARS-CoV-2.
Journal of Tropical Biodiversity and Biotechnology, 2022
Wild bananas provide important genetic materials for further banana improvement, therefore they n... more Wild bananas provide important genetic materials for further banana improvement, therefore they need to be conserved and studied. This study aimed to describe morphological characteristics of plant and seed and also to study the seed germination of wild banana M. acuminata var. flava (Ridl.) Nasution. The morphological characteristics were observed descriptively by referring to the descriptor for banana. The internal and external morphology of the seeds were observed using a digital microscope. The germination testing was carried out by a completely randomized design, using fresh seeds extracted from a bunch of fruits with two ripeness levels i.e. fully-ripe (yellow peel) and under-ripe (green-yellow peel). The data resulted was then analyzed using an independent t-test. The results showed that M. acuminata var. flava is characterized as a perennial herb; pseudostem height ≥3 m; male bud like a top with prominent green-yellow bracts; fruit curved and tasted mild-sweet when ripe. The...
Pomegranate (Punica granatum L.) is a shrub with a height of up to 5 meters belonging to Lythrace... more Pomegranate (Punica granatum L.) is a shrub with a height of up to 5 meters belonging to Lythraceae. It came from Iran and is now spread throughout the world. The use of this plant for traditional medicine has long been known, including by people in Indonesia. Purwodadi Botanical Gardens as one of the ex-situ conservation areas also collects this plant. This writing aims to determine the existence of pomegranate as a collection of the Purwodadi Botanical Gardens, explore the use of pomegranate in traditional medicine in Indonesia, and describe scientific experimental research that supports the potential of pomegranate as a medicine. The data were obtained through a literature study by browsing the collections catalog of the Purwodadi Botanical Gardens, while information on the use of pomegranate as an ingredient in traditional medicine and scientific experimental research on the medical benefits of pomegranate was obtained through relevant journals. Purwodadi Botanical Gardens has four collection numbers of pomegranate, namely XIV.G.I. and XXII.A.I. Pomegranate has long been used in traditional Indonesian medicine, especially in the areas of Madura, Luwu Timur, Baubau, and Bima. Some of the diseases that can be treated with pomegranate include canker sores, fever, intestinal worms infection, hypertension, and cough; and the parts of the plant often used are the fruit and seeds. Scientific studies have proven that pomegranate has oral antibacterial activity so that it can maintain oral health and has potential as an anticancer drug. This information is expected to increase the value of the benefits of pomegranate and encourage the community to participate in conserving it through cultivation efforts.
Perbanyakan vegetatif Hoya melalui stek daun pada tiga macam media, yaitu tanah, pasir, dan cocop... more Perbanyakan vegetatif Hoya melalui stek daun pada tiga macam media, yaitu tanah, pasir, dan cocopeat. Parameter yang diamati meliputi persentase tumbuhnya akar dan jumlah akar
Penyakit-penyakit neurodegeneratif telah menjangkiti jutaan orang di seluruh dunia dengan angka k... more Penyakit-penyakit neurodegeneratif telah menjangkiti jutaan orang di seluruh dunia dengan angka kejadian yang semakin meningkat. Hingga saat ini belum ada pendekatan terapeutik untuk menghentikan perkembangan penyakit-penyakit neurodegeneratif tersebut. Di sisi lain, produk-produk alami, terutama yang berasal dari tumbuhan, memiliki senyawa-senyawa dengan aktivitas anti-neurodegeneratif. Kebun Raya Purwodadi (KRP), sebagai salah satu lembaga konservasi tumbuhan ex situ , diduga menyimpan kekayaan plasma nutfah yang berpotensi sebagai agen neuroproteksi. Penulisan ini bertujuan untuk menginventarisasi koleksi KRP yang berpotensi sebagai agen neuroproteksi melalui studi literatur dan penelusuran katalog KRP. Hasil inventarisasi menunjukkan bahwa sebanyak 53 jenis koleksi KRP dari 21 suku berpotensi sebagai agen neuroproteksi. Lima di antaranya memiliki senyawa aktif lebih dari satu, yaitu Intsia bijuga ( resveratrol & dihydromyricetin ), Syzygium cumini ( kaempferol & dihydromyricetin...
Background: CoVID-19, caused by a new type of coronavirus named SARS-CoV-2, has become a pandemic... more Background: CoVID-19, caused by a new type of coronavirus named SARS-CoV-2, has become a pandemic. Together with SARS-CoV and MERS-CoV, CoVID-19 is a large global outbreak of coronavirus infection; however, its rate of spread is much higher. Since the vaccines and anti-SARS-CoV-2 have not been found, a faster control mechanism is much needed. Traditional herbs have shown the potential for this purpose, as has been demonstrated by the Chinese Government with a high success rate. One of the herbs used was Lindera aggregata, which is part of the collection in Purwodadi Botanic Gardens. Objectives: Through in silico study, this research aims to reveal the secondary metabolites contained in L. aggregata that have the potential to serve as anti-SARS-CoV-2 medication as well as showcase their inhibitory mechanisms. Methods: The research was conducted through molecular docking analysis of terpenoids and alkaloids contained in the root of L. aggregata, with target proteins 3CLpro, PLpro, Spi...
Mangrove forest is located along the coastal area and regularly waterlogged because it is influen... more Mangrove forest is located along the coastal area and regularly waterlogged because it is influenced by the tides, and has an important ecological function for life. Mangrove forest, especially in urban areas, is the most threatened and damaged ecosystem. Surabaya has a mangrove forest in the Mangrove Botanic Garden of Gunung Anyar (MBGGA) with an area of 32,761 m2 and has the potential for educational tourism. Scientific information concerning plant species in MBGGA is needed to support this potential. This study aimed to inventory the mangrove plants and their associated species in MBGGA and their potential as medicinal plants. The inventory was carried out by tracking method while information on medicinal potential was obtained through literature study. The results showed that MBGGA had 14 mangrove species and 11 mangrove-associated species. Around 18 species are included in the least concern category and one in the vulnerable category of the IUCN red list. All plant species in M...
The effect of gibberellin at multiplication stage on somatic embryo growth and maturation and pla... more The effect of gibberellin at multiplication stage on somatic embryo growth and maturation and plantlet regeneration of tangerine (Citrus reticulata Blanco.) var. Batu 55 was assessed. Somatic embryo at globular phase was cultured on MT media + 30 gL-1 sucrose and various concentrations of gibberellin (0, 2, 4, 6, and 8 ppm). The somatic embryo was maturated on MT media + 500 ppm malt extract + 73 mM sorbitol + 73 mM galactose. Cotyledonary somatic embryo was regenerated into plantlet on MT media + 500 ppm malt extract + 30 gL-1 sucrose + 2 ppm GA 3. The results showed that the addition of gibberellin in somatic embryo multiplication stage increased somatic embryo growth and maturation and plantlet regeneration of tangerine. Optimum concentration of gibberellin needed for somatic embryo growth was 4 ppm which yielded twofold fresh weight compared to control. The percentage of maturation was very low below 5%. The addition of gibberellin in media at embryo multiplication stage slightly increased the percentage of maturation about 1-2%. Thirty percent of cotyledonary embryo was able to regenerate into plantlet. The addition of gibberellin in media at embryo multiplication stage increased the regeneration percentage, even the addition of 8 ppm gibberelline yielded regeneration percentage up to 70%.
Physalis angulata L. (Ciplukan) is one member of Solanaceae that has a potential as herbal medici... more Physalis angulata L. (Ciplukan) is one member of Solanaceae that has a potential as herbal medicine. This plant grows wild in the crop fields, forest edges, etc. However, ciplukan is increasingly difficult to find recently. In vitro callus is an alternative source to produce secondary metabolite production as well as to regenerate plants through indirect organogenesis. This study aims to identify the response of hypocotyl explants on in vitro callus formation induced by a combination of auxin and cytokinins. Two types of cytokinins, Kinetin and BAP (0.5 ppm) were combined with three types of auxin, i.e. 2.4-D, IBA and IAA, at three concentrations 0.5, 1.0 and 1.5 ppm. In all combinations of cytokinin and auxin, 50-100% of hypocotyl explants derived from in vitro seedling were able to produce callus either in a compact or watery friable texture. In MS medium supplemented with 2.4-D, callus FW (fresh weight) began to decline in the fourth week after culture. Callus FW that increased until 5 weeks of culture was obtained in medium IAA 0.5 + Kin 0.5, IBA 1.0 + Kin 0.5 and IBA 1 + BA 0.5. Almost all calli induced on a medium + Kinetin also produced roots. While medium + BAP was able to induce shoots regeneration.
Tumbuhan telah dikenal sebagai sumber obat alami yang dapat menyembuhkan berbagai macam penyakit ... more Tumbuhan telah dikenal sebagai sumber obat alami yang dapat menyembuhkan berbagai macam penyakit karena mengandung senyawa metabolit sekunder dengan aktivitas biomedik. Tumbuhan paku merupakan kelompok tumbuhan tertua di bumi yang memiliki lebih dari 12.000 jenis. Tumbuhan paku mengandung senyawa metabolit sekunder yang berpotensi obat berdasarkanstudi etnobotani dan farmakologi. Sebanyak 20 suku dan 38 marga tumbuhan paku dikoleksi dan dikonservasi secara ex situ oleh Kebun Raya (KR) Purwodadi. Nephrolepis merupakan salah satu marga dari koleksi tumbuhan paku KR Purwodadi dengan jumlah spesimen paling banyak. Nephrolepis termasuk kelompok tumbuhan paku epifit atau setengah epifit yang daunnya tersusunmenyirip tunggal seperti pedang. Selama ini Nephrolepis hanya dikenal sebagai bahan sayuran dan tanaman hias oleh masyarakat. Namun belum ada informasi mengenai potensi obat dari koleksi Nephrolepis tersebut. Oleh karena itu, penelitian ini dilakukan untuk menginventarisasi dan mengara...
BromoTenggerSemeruNationalPark(BTSNP)isoneofnaturalconservationareasinEastJavawitharound1,025plan... more BromoTenggerSemeruNationalPark(BTSNP)isoneofnaturalconservationareasinEastJavawitharound1,025plantspecies.Impatiensplatypetala,locallyknownaspacartere,isaplantspeciesmostlybefoundinmountainousareasofJavaandwithhighpotentialasmedicinalandornamentalplant.However,researchonthisspeciesisstilllimited.Thisstudywasconductedtoinventory,describethemorphologicalcharacteristicsandtesttheseedviabilityofpacartereinfourresortsofBTSNP,namelyRPTNGunungPenanjakan,CobanTrisula,Senduro,andRanuDarungan.Surveymethodwasusedtoinventorytheplantpopulations.Directfieldobservationwasusedtocharacterizetheplantmorphology.Seedsviabilitytestwasconductedthroughgerminationonstrawpapermedia.TheresultsshowedthatpacarterepopulationswerefoundinthosefourBTSNPresorts,onshadeandhumidhabitats,alsoinopenareaswithwetsoilconditions.Theplantischaracterizedasanerectparennialherb,spursedpinkflower,capsulafruitwhichdehiscentwhenripe,withflattenedcone-shapedandsmoothhairyseeds.Seedgerminationtestshowedthattheseedsbegantogerminateo...
Biosaintifika: Journal of Biology & Biology Education
Bauhinia winitii Craib (Fabaceae, Caesalpinioideae) is a woody climber which is currently include... more Bauhinia winitii Craib (Fabaceae, Caesalpinioideae) is a woody climber which is currently included in endangered species list. B. winitii seeds are orthodox seeds in which it has hard coat morphologically. Therefore, B. winitii seeds often undergo physical dormancy which can be broken through immersion in certain liquid media. This study aimed to characterize the morphology of B. winitii seeds and determine the pre-sowing treatment method to accelerate the seeds germination of B. winitii. External morphological characterization was carried out by observing the seeds quantitative and qualitative parameters. Characterization of seeds internal morphology was conducted using a digital microscope. Physical dormancy breaking was carried out by immersion in warm and cold water for 24 hours. Morphological characterization data were analyzed descriptively, while seed germination data were analyzed quantitatively by using one-way ANOVA followed by LSD test (with confidence level of 95%). B. ...
Purwodadi Botanic Garden (KR Purwodadi) is an ex situ conservation institution for dry climate lo... more Purwodadi Botanic Garden (KR Purwodadi) is an ex situ conservation institution for dry climate lowland plants aiming at saving and protecting biodiversity from extinction. In supporting ex situ conservation efforts, plant exploration activities in their natural habitat need to be carried out. Alas Purwo National Park has a lowland forest area with abundant plant diversity. In addition to functioning as a conservation forest, this area is also a natural and religious tourist attraction, so it is likely to undergo ecosystem damage. Therefore, this plant diversity study with the activity of plants exploration and collection was carried out for the purpose of ex situ conservation in KR Purwodadi. This study was conducted in August to September 2019 using an explorative method. A total of 24 species were obtained, of which 8 species were not yet cultivated by KR Purwodadi. Besides, there were 5 species included in the IUCN red list, namely Aglaonema simplex, Brucea javanica, Cycas rumphi...
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