The present study was conducted at Gangajalghati, a village near the forest of Bankura district f... more The present study was conducted at Gangajalghati, a village near the forest of Bankura district from West Bengal that has a tropical wet and dry climate where moth diversity has not been explored before. The village was surveyed between January 2016 and December 2018. The present study has recorded a total of 1,328 individual moths belonging to 13 families, 31 subfamilies, 80 genera, and 90 species. Three species—Condylorrhiza diniasalis (Walker, 1859), Argyrocosma inductaria (Guenée, 1858), and Oraesia emarginata (Fabricius, 1794)—are reported for the first time from West Bengal and Eublemma roseonivea (Walker, 1863) shows its westernmost distribution in West Bengal, India. It was earlier reported from India (Assam), China, Taiwan, the Philippines, Malaya, and Borneo.
A double-blind controlled field trial of doxycycline and albendazole in combination for the treat... more A double-blind controlled field trial of doxycycline and albendazole in combination for the treatment of bancroftian filariasis in India, Acta Tropica (2010), doi:10.1016/j.actatropica.2012.10.011 This is a PDF file of an unedited manuscript that has been accepted for publication. As a service to our customers we are providing this early version of the manuscript. The manuscript will undergo copyediting, typesetting, and review of the resulting proof before it is published in its final form. Please note that during the production process errors may be discovered which could affect the content, and all legal disclaimers that apply to the journal pertain.
A bio-assay guided fractionation and purification approach was used to examine in vitro antifilar... more A bio-assay guided fractionation and purification approach was used to examine in vitro antifilarial activities of the crude methanolic extract of Nyctanthes arbortristis as well as fractions and isolated compound. From ethyl-acetate fraction we isolated and identified a triterpenoid compound which has been characterized as ursolic acid (UA) by HPLC and NMR data. We are reporting for the first time isolation and identification of UA from the leaves of N. arbortristis. The crude extract and UA showed significant micro- as well as macrofilaricidal activities against the oocyte, microfilaria and adult of Setaria cervi (S. cervi) by dye exclusion test and MTT reduction assay. Significant microfilaricidal activity of UA was further proved against mf of W. bancrofti by viability assay. The findings thus provide a new lead for development of a suitable filaricide from natural products. The molecular mechanism of UA was investigated by performing TUNEL, Hoechst staining, Annexin V-Cy3, flow cytometric analysis and DNA fragmentation assay. Differential expressions of pro- and anti-apoptotic genes were observed at the transcription and translational levels in a dose-dependent manner. Depletion in the worm GSH level and elevation in the parasite GST, SOD and super oxide anion indicated the generation of ROS. In this investigation we are reporting for the first time that UA acts its antifilarial effect through induction of apoptosis and by downregulating and altering the level of some key antioxidants like GSH, GST and SOD of S. cervi.
In the reported work the in vitro activity of a methanolic extract of leaves of Hibiscus mutabili... more In the reported work the in vitro activity of a methanolic extract of leaves of Hibiscus mutabilis (Malvaceae) against bovine Setaria cervi worms has been investigated. Bioassay-guided fractionation led to isolation of ferulic acid from ethyl acetate fraction. The crude extract and ferulic acid, the active molecule, showed significant microfilaricidal as well as macrofilaricidal activities against the microfilaria (L 1) and adult of S. cervi by both a worm motility and MTT reduction assay. The findings thus provide a new lead for development of a filaricidal drug from natural products. To examine the possible mechanism of action of ferulic acid, the involvement of apoptosis in adult worms of S. cervi was investigated. We found extreme cellular disturbances in ferulic acid-treated adult worms characterized by chromatin condensation, in situ DNA fragmentation and nucleosomal DNA laddering. In this work we are reporting for the first time that ferulic acid exerts its antifilarial effect through induction of apoptosis and by downregulating and altering the level of some key antioxidants (GSH, GST and SOD) of the filarial nematode S. cervi. Our results have provided experimental evidence supporting that ferulic acid causes an increased proapoptotic gene expression and decreased expression of anti-apoptotic genes simultaneously with an elevated level of ROS and gradual dose dependent decline of parasitic GSH level. We also observed a gradual dose dependent elevation of GST and SOD activity in the ferulic acid treated worms.
A study was conducted at the Banaras Hindu University (BHU) campus of Varanasi, Uttar Pradesh, In... more A study was conducted at the Banaras Hindu University (BHU) campus of Varanasi, Uttar Pradesh, India to assess the moth fauna of the area. A preliminary checklist was compiled as a base-line contribution to the status of the Lepidoptera diversity of the campus. The campus was surveyed from January to December 2019 and moths were recorded through 83-night surveys and a large number of opportunistic visits in 18 different sites of the campus. The study has recorded a total of 1248 individual moths belonging to 99 morphospecies, 84 genera, and 11 families across different parts of the study area. The most species rich family was Erebidae with 35 species under 30 genera followed by Crambidae (33 species; 28 genera), Geometridae (15 species; 11 genera), Noctuidae (seven species; six genera), and others. However, family-wise abundance data indicated that Crambidae (38.70%) was the most abundant family having highest proportion of moths recorded followed by Erebidae (34.85%), Geometridae (...
Your article is protected by copyright and all rights are held exclusively by Springer-Verlag. Th... more Your article is protected by copyright and all rights are held exclusively by Springer-Verlag. This e-offprint is for personal use only and shall not be self-archived in electronic repositories. If you wish to self-archive your work, please use the accepted author's version for posting to your own website or your institution's repository. You may further deposit the accepted author's version on a funder's repository at a funder's request, provided it is not made publicly available until 12 months after publication.
Your article is protected by copyright and all rights are held exclusively by Springer-Verlag. Th... more Your article is protected by copyright and all rights are held exclusively by Springer-Verlag. This e-offprint is for personal use only and shall not be self-archived in electronic repositories. If you wish to self-archive your work, please use the accepted author's version for posting to your own website or your institution's repository. You may further deposit the accepted author's version on a funder's repository at a funder's request, provided it is not made publicly available until 12 months after publication.
Setaria cervi, a filarial nematode of cattle, inhabits in the peritoneal cavity and has been used... more Setaria cervi, a filarial nematode of cattle, inhabits in the peritoneal cavity and has been used as a suitable model for screening antifilarial agents. Albendazole (ABZ), a tubulin-disrupting benzimidazole (BZ) and a potent microfilaricide binds to b-tubulin, is causing structural impairment of cytoskeleton and worm death. Our present study has revealed that exposure of microfilaria (Mf) and adult to gradually increasing concentration of ABZ leads to a dose-dependent gradual impairment of their motility followed by early death in vitro. We found extreme cellular disturbances in ABZ-treated worms characterized by nucleosomal DNA laddering and chromatin condensation. However, in the treated Mf no nucleosomal DNA laddering was found although presence of TUNEL reactive DNA was evident, thus indicating an apoptotic pathway independent of DNA fragmentation. We present data from molecular studies to provide evidence for ABZ-induced apoptosis in Mf and adult worms of S. cervi.
The present study was conducted at Gangajalghati, a village near the forest of Bankura district f... more The present study was conducted at Gangajalghati, a village near the forest of Bankura district from West Bengal that has a tropical wet and dry climate where moth diversity has not been explored before. The village was surveyed between January 2016 and December 2018. The present study has recorded a total of 1,328 individual moths belonging to 13 families, 31 subfamilies, 80 genera, and 90 species. Three species—Condylorrhiza diniasalis (Walker, 1859), Argyrocosma inductaria (Guenée, 1858), and Oraesia emarginata (Fabricius, 1794)—are reported for the first time from West Bengal and Eublemma roseonivea (Walker, 1863) shows its westernmost distribution in West Bengal, India. It was earlier reported from India (Assam), China, Taiwan, the Philippines, Malaya, and Borneo.
A double-blind controlled field trial of doxycycline and albendazole in combination for the treat... more A double-blind controlled field trial of doxycycline and albendazole in combination for the treatment of bancroftian filariasis in India, Acta Tropica (2010), doi:10.1016/j.actatropica.2012.10.011 This is a PDF file of an unedited manuscript that has been accepted for publication. As a service to our customers we are providing this early version of the manuscript. The manuscript will undergo copyediting, typesetting, and review of the resulting proof before it is published in its final form. Please note that during the production process errors may be discovered which could affect the content, and all legal disclaimers that apply to the journal pertain.
A bio-assay guided fractionation and purification approach was used to examine in vitro antifilar... more A bio-assay guided fractionation and purification approach was used to examine in vitro antifilarial activities of the crude methanolic extract of Nyctanthes arbortristis as well as fractions and isolated compound. From ethyl-acetate fraction we isolated and identified a triterpenoid compound which has been characterized as ursolic acid (UA) by HPLC and NMR data. We are reporting for the first time isolation and identification of UA from the leaves of N. arbortristis. The crude extract and UA showed significant micro- as well as macrofilaricidal activities against the oocyte, microfilaria and adult of Setaria cervi (S. cervi) by dye exclusion test and MTT reduction assay. Significant microfilaricidal activity of UA was further proved against mf of W. bancrofti by viability assay. The findings thus provide a new lead for development of a suitable filaricide from natural products. The molecular mechanism of UA was investigated by performing TUNEL, Hoechst staining, Annexin V-Cy3, flow cytometric analysis and DNA fragmentation assay. Differential expressions of pro- and anti-apoptotic genes were observed at the transcription and translational levels in a dose-dependent manner. Depletion in the worm GSH level and elevation in the parasite GST, SOD and super oxide anion indicated the generation of ROS. In this investigation we are reporting for the first time that UA acts its antifilarial effect through induction of apoptosis and by downregulating and altering the level of some key antioxidants like GSH, GST and SOD of S. cervi.
In the reported work the in vitro activity of a methanolic extract of leaves of Hibiscus mutabili... more In the reported work the in vitro activity of a methanolic extract of leaves of Hibiscus mutabilis (Malvaceae) against bovine Setaria cervi worms has been investigated. Bioassay-guided fractionation led to isolation of ferulic acid from ethyl acetate fraction. The crude extract and ferulic acid, the active molecule, showed significant microfilaricidal as well as macrofilaricidal activities against the microfilaria (L 1) and adult of S. cervi by both a worm motility and MTT reduction assay. The findings thus provide a new lead for development of a filaricidal drug from natural products. To examine the possible mechanism of action of ferulic acid, the involvement of apoptosis in adult worms of S. cervi was investigated. We found extreme cellular disturbances in ferulic acid-treated adult worms characterized by chromatin condensation, in situ DNA fragmentation and nucleosomal DNA laddering. In this work we are reporting for the first time that ferulic acid exerts its antifilarial effect through induction of apoptosis and by downregulating and altering the level of some key antioxidants (GSH, GST and SOD) of the filarial nematode S. cervi. Our results have provided experimental evidence supporting that ferulic acid causes an increased proapoptotic gene expression and decreased expression of anti-apoptotic genes simultaneously with an elevated level of ROS and gradual dose dependent decline of parasitic GSH level. We also observed a gradual dose dependent elevation of GST and SOD activity in the ferulic acid treated worms.
A study was conducted at the Banaras Hindu University (BHU) campus of Varanasi, Uttar Pradesh, In... more A study was conducted at the Banaras Hindu University (BHU) campus of Varanasi, Uttar Pradesh, India to assess the moth fauna of the area. A preliminary checklist was compiled as a base-line contribution to the status of the Lepidoptera diversity of the campus. The campus was surveyed from January to December 2019 and moths were recorded through 83-night surveys and a large number of opportunistic visits in 18 different sites of the campus. The study has recorded a total of 1248 individual moths belonging to 99 morphospecies, 84 genera, and 11 families across different parts of the study area. The most species rich family was Erebidae with 35 species under 30 genera followed by Crambidae (33 species; 28 genera), Geometridae (15 species; 11 genera), Noctuidae (seven species; six genera), and others. However, family-wise abundance data indicated that Crambidae (38.70%) was the most abundant family having highest proportion of moths recorded followed by Erebidae (34.85%), Geometridae (...
Your article is protected by copyright and all rights are held exclusively by Springer-Verlag. Th... more Your article is protected by copyright and all rights are held exclusively by Springer-Verlag. This e-offprint is for personal use only and shall not be self-archived in electronic repositories. If you wish to self-archive your work, please use the accepted author's version for posting to your own website or your institution's repository. You may further deposit the accepted author's version on a funder's repository at a funder's request, provided it is not made publicly available until 12 months after publication.
Your article is protected by copyright and all rights are held exclusively by Springer-Verlag. Th... more Your article is protected by copyright and all rights are held exclusively by Springer-Verlag. This e-offprint is for personal use only and shall not be self-archived in electronic repositories. If you wish to self-archive your work, please use the accepted author's version for posting to your own website or your institution's repository. You may further deposit the accepted author's version on a funder's repository at a funder's request, provided it is not made publicly available until 12 months after publication.
Setaria cervi, a filarial nematode of cattle, inhabits in the peritoneal cavity and has been used... more Setaria cervi, a filarial nematode of cattle, inhabits in the peritoneal cavity and has been used as a suitable model for screening antifilarial agents. Albendazole (ABZ), a tubulin-disrupting benzimidazole (BZ) and a potent microfilaricide binds to b-tubulin, is causing structural impairment of cytoskeleton and worm death. Our present study has revealed that exposure of microfilaria (Mf) and adult to gradually increasing concentration of ABZ leads to a dose-dependent gradual impairment of their motility followed by early death in vitro. We found extreme cellular disturbances in ABZ-treated worms characterized by nucleosomal DNA laddering and chromatin condensation. However, in the treated Mf no nucleosomal DNA laddering was found although presence of TUNEL reactive DNA was evident, thus indicating an apoptotic pathway independent of DNA fragmentation. We present data from molecular studies to provide evidence for ABZ-induced apoptosis in Mf and adult worms of S. cervi.
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