Papers by Andrea Mantesso
Acta Cirurgica Brasileira, 2013
To analyze the effects of aqueous ozone irrigation over bone healing in hyperglycemia-induced rats.
Oncotarget, 2015
A small sub-population of cells characterized by increased tumorigenic potential, ability to self... more A small sub-population of cells characterized by increased tumorigenic potential, ability to self-renew and to differentiate into cells that make up the tumor bulk, has been characterized in some (but not all) tumor types. These unique cells, namedcancer stem cells, are considered drivers of tumor progression in these tumors. The purpose of this work is to understand if cancer stem cells play a functional role in the tumorigenesis of salivary gland mucoepidermoid carcinomas. Here, we investigated the expression of putative cancer stem cell markers (ALDH, CD10, CD24, CD44) in primary human mucoepidermoid carcinomas by immunofluorescence, in vitro salisphere assays, and in vivo tumorigenicity assays in immunodeficient mice. Human mucoepidermoid carcinoma cells (UM-HMC-1, UM-HMC-3A, UM-HMC-3B) sorted for high levels of ALDH activity and CD44 expression (ALDHhighCD44high) consistently formed primary and secondary salispheres in vitro, and showed enhanced tumorigenic potential in vivo (defined as time to tumor palpability, tumor growth after palpability), when compared to ALDHlowCD44low cells. Cells sorted for CD10/CD24, and CD10/CD44 showed varying trends of salisphere formation, but consistently low in vivo tumorigenic potential. And finally, cells sorted for CD44/CD24 showed inconsistent results in salisphere formation and tumorigenic potential assays when different cell lines were evaluated. Collectively, these data demonstrate that salivary gland mucoepidermoid carcinomas contain a small population of cancer stem cells with enhanced tumorigenic potential and that are characterized by high ALDH activity and CD44 expression. These results suggest that patients with mucoepidermoid carcinoma might benefit from therapies that ablate these highly tumorigenic cells.
O objetivo deste trabalho foi determinar a capacidade de um programa de subtração radiográfica em... more O objetivo deste trabalho foi determinar a capacidade de um programa de subtração radiográfica em detectar pequenas alterações ósseas alveolares. Defeitos medindo 1,55 mm de diâmetro foram confeccionados na crista óssea alveolar interproximal, mesial e distalmente ao segundo molar superior, em 3 crânios humanos. Esses defeitos foram progressivamente aumentados para 2,46 mm e 2,86 mm de diâmetro. Radiografias padronizadas foram tomadas antes da criação dos defeitos e após cada ampliação dos mesmos defeitos. Em seguida, elas foram processadas automaticamente e digitalizadas. As imagens foram corrigidas quanto a diferença nos tons de cinza e analisadas por meio de um programa específico para subtração de imagens. As imagens subtraídas foram interpretadas em triplicata por um examinador treinado. A sensibilidade e especificidade foram calculadas, assim como a correlação entre o tamanho real do defeito e o tamanho estimado pelo programa. A sensibilidade e especificidade do examinador em detectar os defeitos foram, respectivamente, 66,67% e 75%, para defeitos pequenos (1,55 mm) na distal do molar e 100% para os demais defeitos. Houve uma forte correlação entre o tamanho real do defeito e o tamanho estimado pelo programa (r 2 = 0,88, p < 0,0001). Um aumento de 0,4 mm do defeito não resultou em mudança no nível de cinza detectável pelo programa. Concluindo, o programa foi preciso em correlacionar o tamanho real do defeito com o tamanho estimado, entretanto, uma mudança óssea de 0,4 mm no tamanho do defeito não foi diagnosticada como mudança no nível de cinza e a sensibilidade e especificidade foram baixas para defeitos pequenos na distal dos molares. DESCRITORES Periodontia. Radiografia digital. In vitro.
International Journal of Case Reports and Images, 2013
The Journal of clinical pediatric dentistry, 2001
Hand-Schuller-Christian disease is a multifocal variant of eosinophilic granuloma, characterised ... more Hand-Schuller-Christian disease is a multifocal variant of eosinophilic granuloma, characterised by the classical triad of bony lesions, exophthalmos and diabetes insipidus. This case relates recurrent Langerhans' cell histiocytosis lesions presented as destruction of periodontal support associated with diabetes in a 10-year-old patient. Medical history suggests that the case represents a case of Hand-Schuller Christian disease.
Oral Surgery, Oral Medicine, Oral Pathology and Oral Radiology, 2014
General dentistry
Chondrosarcoma is a malignant cartilaginous tumor that rarely occurs in the maxillofacial bones. ... more Chondrosarcoma is a malignant cartilaginous tumor that rarely occurs in the maxillofacial bones. A 44-year-old woman complained about swelling and mild pain during mastication in the right parasymphysis region. Clinical and radiographic examinations revealed characteristics of osteosarcoma. A microscopic examination revealed an abundant proliferation of malignant neoplastic cartilage cells of varying sizes arranged as immature tissue and the absence of an osteoid matrix. This article presents a case of chondrosarcoma of the jaw and discusses the differences between osteosarcoma and chondrosarcoma.
Quintessence international (Berlin, Germany : 1985)
To analyze the expression of tenascin, fibronectin, collagens I and III, osteonectin, and bone mo... more To analyze the expression of tenascin, fibronectin, collagens I and III, osteonectin, and bone morphogenetic protein 4 (BMP4) in the extracellular matrix of pulp tissue in primary teeth during physiologic root resorption. Eighteen teeth were decalcified and equally distributed into 3 groups (group I, teeth with two-thirds root length; group II, teeth with one-third root length; and group III, teeth lacking the root). Immunohistochemical analysis showed that all the proteins were expressed. Tenascin, collagen I, and osteonectin showed strong and broad reactivity in group I, with weaker and rare reactivity in groups II and III. The expression of fibronectin, collagen III, and BMP4 did not vary with root resorption phase. The expression of tenascin, collagen I, and osteonectin was reduced in the extracellular matrix and odontoblasts during root resorption. This fact may be related to the decreasing pulp response to damage and treatment during the progression of root resorption.
Brazilian Oral Research, 2011
Vimentin is a cytoeskeletal intermediate filament protein commonly observed in mesenchymal cells;... more Vimentin is a cytoeskeletal intermediate filament protein commonly observed in mesenchymal cells; however, it can also be found in malignant epithelial cells. It is demonstrated in several carcinomas, such as those of the cervix, breast and bladder, in which it is widely used as a marker of the epithelial to mesenchymal transition that takes place during embryogenesis and metastasis. Vimentin is associated with tumors that show a high degree of invasiveness, being detected in invasion front cells. Its expression seems to be influenced by the tumor microenvironment. The aim of this study was to evaluate vimentin expression in head and neck squamous cell carcinoma (HNSCC) cell lines, and to investigate the contribution of the microenvironment to its expression. HNSCC cell lines (HN6, HN30 and HN31) and an immortalized nontumorigenic cell line (HaCaT) were submitted to a three-dimensional assay with Matrigel. Cytoplasmatic staining of the HN6 cell line cultured without Matrigel and of the HN30 and HN31 cell lines cultured with Matrigel was demonstrated through immunohistochemistry. Western Blotting revealed a significant decrease in vimentin expression for the HN6 cell line and a significant increase for the HN30 and HN31 cell lines cultured with Matrigel. The results suggest that vimentin can be expressed in HNSCC cells and its presence is influenced by the microenvironment of a tumor.
Brazilian Oral Research, 2014
Dental pulp has been identified as a novel and promising stem cell source. The following systemat... more Dental pulp has been identified as a novel and promising stem cell source. The following systematic review presents and summarises in vivo studies that have used stem cells from the dental pulp of permanent and deciduous teeth to repair or regenerate non-dental tissues. An electronic customised search was performed using 4 different databases (Entrez PubMed, Cab Abstracts, Scopus and Web of Science). Only full-text research manuscripts published in English between the years of 2000 and 2012 were included. The manuscripts were retrieved based on the following keywords and/or abbreviations: [Stem Cells from Human Exfoliated Deciduous teeth (SHED)] AND/OR [Dental Pulp Stem Cells (DPSC)] AND [tissue regeneration] AND [tissue repair]. Only manuscripts involving in vivo applications of SHED or DPSC for the repair and/or regeneration of non-dental tissues were included. The search strategy produced 2309 papers, from which 14 were eligible according to the predetermined inclusion and exclusion criteria. Although human tissue was the source of cells in half of the studies included in our review, all of the studies involved transplantation into animals of other species, such as pigs, rats and mice. Most of the manuscripts reported the successful use of DPSCs or SHED for non-dental tissue repair or regeneration. While these cell populations represent promising alternative sources of stem cells for tissue engineering and cell-based regenerative medicine therapies, it is not yet possible to guarantee the appropriate clinical management of this technique.
Acta Cirurgica Brasileira, 2013
To analyze the effects of aqueous ozone irrigation over bone healing in hyperglycemia-induced rats.
BMC Research Notes, 2014
Background: Gingival hyperplasias are peculiar conditions that may produce extreme growth that im... more Background: Gingival hyperplasias are peculiar conditions that may produce extreme growth that impairs masticatory function and causes psychological and aesthetic disturbances. They can vary from mild interdental papillae localized growth to marked swelling affecting both jaws. Case presentation: The aim of this case report is to present a rare case of generalized gingival growth diagnosed in a 4 year-old Caucasian child and followed for 9 years. The lesion covered almost all of the upper and lower teeth and recurred thirty times with the same clinical and histopathological aspects. The clinical features suggested the diagnosis of idiopathic gingival fibromatosis, but the histopathological aspects did not confirm this hypothesis and were consistent with peripheral ossifying fibroma. Conclusion: The present case reports a rare gingival growth with challenging diagnosis and treatment.
Oral Diseases, 2000
Paracoccidioidomycosis is an important deep mycosis, endemic in some areas of the South American ... more Paracoccidioidomycosis is an important deep mycosis, endemic in some areas of the South American countryside, with great incidence in males bearing rural activities, being unusual in urban developed centres. Cell-mediated immunity is the main host defence against the P. brasiliensis, and HIV-positive patients have been increasingly affected by the disease, although only a few reports are available in the literature. We present a case of paracoccidiodomicosis in an HIV-positive female, with unusual clinical history and histopathological aspects.
Otolaryngology - Head and Neck Surgery, 2007
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Papers by Andrea Mantesso