John Ihedioha

University of Nigeria, Nsukka, Veterinary Pathology and Microbiology, Faculty Member

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Phone: +2348035387156
Address: Department of Veterinary Pathology and Microbiology, Faculty of Veterinary Medicine, University of Nigeria, Nsukka, Enugu State, Nigeria.

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Papers by John Ihedioha

Hematological and serum biochemical profiles of a natural African swine fever virus infection in pigs

PubMed, Dec 31, 2022

African swine fever (ASF) is a contagious viral disease that affects pigs of all ages, inducing h... more African swine fever (ASF) is a contagious viral disease that affects pigs of all ages, inducing hemorrhagic fever with high mortality and severe threat to pig production. This study investigated the hematological and serum biochemical abnormalities associated with a natural ASF infection in pigs. A total of 100 serum samples of pigs from piggery suspected of ASFV infection were screened for antibodies by ELISA. Thirty‑two blood samples from serologically positive pigs and 32 negative pigs were undergo to hematological and serum biochemical analyses following standard procedures. The results showed that the mean values of the red blood cell (RBC) count, total white blood cell (TWBC) count, absolute lymphocyte count, absolute monocyte count, serum total protein (TP) and globulin were significantly (p < 0.05) lower while the mean corpuscular volume (MCV), mean corpuscular hemoglobin concentration (MCHC), absolute neutrophil count and serum gamma glutamyl transferase (GGT) were significantly (p < 0.05) higher in the infected than the healthy pigs. There were no significant differences (p > 0.05) in the mean values of the packed cell volume (PCV), hemoglobin concentration, absolute eosinophil count, cholesterol, alanine aminotransferase (ALT) and aspartate aminotransferase (AST) activities between the infected and healthy pigs. Hence, natural ASFV infection may have caused alterations in the hematological and serum biochemical parameters in the infected pigs. The generated data could complement the existing laboratory diagnostic techniques such as polymerase chain reaction, direct fluorescence antibody test, indirect fluorescent antibody test and ELISA in the diagnosis of ASF in pigs.

Demographic Profiling of Dogs and Dog Ownership in Nsukka Metropolis of Enugu State, Nigeria: a Case study using the Free Anti-Rabies Vaccination Campaign conducted at the Veterinary Teaching Hospital of the University of Nigeria, Nsukka: January-April, 2015

American journal of agricultural research, 2018

This Dog ownership in Nigeria, patterns a combination of supervised and stray dogs. The latter co... more This Dog ownership in Nigeria, patterns a combination of supervised and stray dogs. The latter constituting Public health challenges. This study relates ecology; dynamics of dog breeds, ownership, management; adherence/non-adherence to extant laws on dog ownership with Rabies endemicity in Nsukka. It proffers recommendations for sustainable Rabies control & vaccination campaign Key Message Nsukka is endemic for Rabies. Statutory control and prevention strategies do not suffice. Understanding the human-dog-interactions as core risk factors for endemicity of Rabies and breaking this link can lead to a Rabies free environment. Abbreviations: FAO: Food and Agricultural Organisation; DFID: Department for International Development; PME: Planning Monitoring and Evaluation, WHO: World Health Organisation

Haematological and Serum Biochemical Changes in Chronic Laminitis Affected Horses

Indian Journal of Animal Research, Aug 17, 2020

Background: Equine laminitis is considered as one of the major health problems affecting equine s... more Background: Equine laminitis is considered as one of the major health problems affecting equine species. Haematological and serum biochemical assessments are often used for diagnosis of both human and animal diseases. Despite numerus findings on induced acute laminitis in horses, little is known about the haematological and serum biochemical changes associated with chronic laminitis. The current study aimed to evaluate the hematological and biochemical changes in horses with chronic laminitis. Methods: A total of 60 horses comprising of 22 apparently healthy horses (group A) and 38 horses radiographically diagnosed as laminitis (group B) were studied during 2018-2019 laboratory investigation. Blood collection for hematology and serum biochemistry were carried out aseptically via the jugular vein. The packed cell volume (PCV) and hemoglobin concentration (Hb) were determined using micro-haematocrit and cyanomethaemoglobin methods respectively. White blood cell (WBC) and red blood cell (RBC) counts were determined using haemocytometer method. Serum alanine aminotransferase (ALT) and aspartate aminotransferase (AST) activities were determined by the Reitman-Frankel method. Serum alkaline phosphatase (ALP) activity, total protein, albumin and creatinine were determined using phenolphthalein monophosphate, biuret, bromocresol green and modified Jaffe methods respectively. Parameters were analyzed using student t-test. Results: The PCV, HB, RBC, WBC and neutrophil counts in groups B were significantly lower compared to group A (control). Lymphocytes, monocytes, basiophils, eosinophils and the biochemical parameters in the control group (group A) horses did not vary significantly (P greater than 0.05) with group B (horses with laminitis). The haematological profiles depict anaemic state of the laminitis in horses. Non-differing enzymatic activities in the two groups could suggest that there may not be muscular, renal, intestinal and hepatic disorders involvement with the occurrence of chronic laminitis in the assessed horses.

Radiographic morphometry of the hoof and third phalanx of apparently healthy Nigerian horses

Indian Journal of Animal Research, Mar 25, 2018

The objective of the study was to establish reference values for radiographic soft tissue indices... more The objective of the study was to establish reference values for radiographic soft tissue indices of the hooves of apparently healthy Nigerian horses and to identify sex and age-related variations if any. Lateromedial radiographic examinations of the forelimb hooves of 52 apparently healthy Nigerian horses of both sexes aged young (1-5years), adult (6-10years) and old (11years and above) were carried out in the cross-sectional survey design. Radiographic soft tissue indices such as hoof wall distal phalanx distance (HWDPD), hoof wall proximal phalanx distance (HWPPD), coronary extensor distance (CED), sole depth (SD), hoof cup (HC), palmarocortical length (PCL) of the third phalanx, hoof wall angle (HWA), angle of third phalanx (AP3) and angle of rotation (AR) were measured and compared between sexes and among age groups. There was no significant sex variations in the indices investigated. However, the mean SD and HC showed age-related significant variation. The mean SD was only significantly (P < 0.05) lower in old horses compared to young and adult horses. The mean values of HC were significantly (P < 0.05) lower in adult and old horses compared to young horses. Radiographic morphometric indices assessed in this study may differ with those reported from other breeds, so they can be used in the future as reference values for diagnosis of laminitis in front feet of Nigerian horses.

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