Papers by PULIPATI SOWJANYA
African Journal of Biological Sciences, 2024
This research looked into how well Aloe vera juice works as an antioxidant in the production of s... more This research looked into how well Aloe vera juice works as an antioxidant in the production of selenium nanoparticles. A UV-visible spectrophotometer, Transmission electron microscopy and the ABTS and DPPH tests were used to do the testing. The UV-Vis spectrum of aloe vera extract at 350 nm shows that it contains round and hollow particles with sizes between 7 and 48 nm. These particles are more stable over longer periods of time. The results from the ABTS and DPPH tests show that the aloe vera extract that was used to make selenium nanoparticles worked better than the extract that was used in its original form. Selenium nanoparticles were made when proteins in the Aloe vera extract joined together to form a cap and keep the particles stable. Even though it is poisonous, selenite has antioxidant qualities and is now being used as a chemopreventive drug to help find cancer. Selenium that is in the form of elements or nanoparticles is not as dangerous.
International Journal of Pharmaceutical Research & Allied Sciences, 2024
Preclinical testing hepatoprotective potential of hydroalcoholic leaf extract of Calycopteris flo... more Preclinical testing hepatoprotective potential of hydroalcoholic leaf extract of Calycopteris floribunda against
liver damage caused by atorvastatin and clopidogrel. Atorvastatin and clopidogrel were delivered
intraperitoneally for 14 days at 80mg/kg and 20mg/kg respectively, while leaf extract was given orally for the
same period at 200mg/kg and 400mg/kg. To evaluate the impact of the leaf extract on the atorvastatin and
clopidogrel-induced liver damage, many biochemical markers, Serum glutamate pyruvate transaminase (SGPT),
serum glutamate oxaloacetate transaminase (SGOT), total protein, and serum alkaline phosphatase were
measured. Blood samples from mice given hydroalcoholic Calycopteris floribunda leaf extract showed a
noteworthy decrease in serum indicators, suggesting that the leaf extract had an impact on the hepatocytes' ability
to operate normally again. On the other hand, the mice receiving atorvastatin and clopidogrel treatment showed
increased levels, which suggested serious liver injury. Silymarin (100 mg/kg) was given as a benchmark. The
current investigation found that Calycopteris floribunda hydroalcoholic extract significantly protected the liver
against atorvastatin and clopidogrel-induced hepatic damage.
Asian Journal of Chemistry, 2024
Seven semicarbazone derivatives incorporating either substituted furan or thiophene were synthesi... more Seven semicarbazone derivatives incorporating either substituted furan or thiophene were synthesized by reacting phenylurea with semicarbazide and various heteroaryl aldehydes. The newly synthesized compounds were characterized using IR, NMR, mass spectrometry and elemental analysis. Additionally, their antibacterial and anti-tubercular activities were also evaluated. In silico tools such as Swiss ADME and Pro Tox II were employed to assess molecular targets, medicinal chemistry properties, as well as toxicity and safety profiles. Compounds 3a-g demonstrated significant antibacterial activity at a 100 µg/mL when compared to the standard ciprofloxacin. Notably, compounds 3c, 3d, 3f and 3g exhibited the highest activity against all bacterial strains. Furthermore, compound 3d demonstrated antitubercular activity at all tested concentrations.
International Journal of Creative Research Thoughts (IJCRT), 2023
The WHO classified COVID-19 a global pandemic, and its emergence has caused a disaster in global ... more The WHO classified COVID-19 a global pandemic, and its emergence has caused a disaster in global public health. Vitamin D is one of the main micronutrients being researched for its potential to have a preventive effect against the COVID-19 condition. Serum 25-hydroxyvitamin D (25(OH)D) concentrations and the risk or severity of coronavirus disease 2019 (COVID-19) have been found to be inversely related in observational studies. The significance of vitamin D in COVID-19 has been attributed to a number of pathways, such as the modulation of immunological and inflammatory responses, control of the renin-angiotensin-aldosterone system, and participation in glucose metabolism and the cardiovascular system. Vitamin D supplementation aids to lower the risk of severe disease progression of patients with COVID-19. In order to better understand the potential impact of vitamin D insufficiency on COVID-19 status and health outcomes in COVID-19 patients, this systematic review aims to find and consolidate the relevant literature.
Journal of Advanced Zoology, 2023
Background and Objective: The tiny, fragrant shrub Artemisia
nilagirica (Clarke) is a member of t... more Background and Objective: The tiny, fragrant shrub Artemisia
nilagirica (Clarke) is a member of the Asteraceae family. This plant's
leaves and flowering top have been used in traditional medicine as an
antimalarial, nerve tonic, and anticancer drug, and there is evidence of
anti-leishmanial efficacy. Material and Methods: The plant specimen
was obtained from the Ooty district in Tamil Nadu, India, during the
month of December. It was subsequently authenticated by NMKRV
College for Women, Bengaluru, Karnataka, India. The plant
specimens were stored in the herbarium of the NMKRV College for
Women, Bengaluru, Karnataka, India. Results: The morphological,
microscopic, organoleptic, and other WHO-recommended procedures
for standardization were investigated in a powdered sample of
Artemisia nilagirica (Clarke) leaf. Phytochemical investigation
revealed the existence of numerous elements such as alkaloids, amino
acids, carbohydrates, flavonoids, glycosides, terpenoids, steroids,
saponins, essential oils, tannins and phenol in diverse extracts.
Conclusion: Pharmacognostic and phytochemical analysis of
Artemisia nilagirica (Clarke) leaves would be useful for authenticating
raw material or crude medication.
Journal of Chemical Health Risks, 2023
There is a rapidly expanding discipline dedicated to the creation of non-biodegradable nanopartic... more There is a rapidly expanding discipline dedicated to the creation of non-biodegradable nanoparticles for use in nanomedicine and nanotechnology. The purpose of this study is to create and test the efficacy of colloidal silver nanoparticles as an antibacterial and antifungal agent. Standard chemical reduction techniques have been used to prepare the colloidal silver nanoparticles. The nanoparticles of colloidal silver were studied using TEM, zeta potential, photo correlation spectroscopy, and in vitro release kinetics. The resulting particles were spherical, with an average particle size between 5 and 50 nm and zeta potentials between -10.0 and -30.0 mV; their release kinetics followed zero-order kinetics with a correlation coefficient of 0.96 or higher. According to the results of the dissolution tests, the release of silver nanoparticles is proportional to their size, i.e., the release is higher for smaller particles. These findings point to the stability of Ag NPs in pharmaceutical formulations and their potential for rapid delivery to the site of infection. There was a wide range of bacterial species that were killed by the colloidal silver nanoparticles. Bacteria such as Escherichia coli, Salmonella, and Pseudomonas aeruginosa were the focus of the research. Aspergillus and Penicillium's antifungal properties were also studied. Nanoparticle cytotoxicity was investigated in human fibroblast cells. The results indicated that cytotoxicity depends on concentration. In light of these findings, silver nanoparticles may be worth investigating further as a potential antibacterial and antifungal agent that would avoid the drawbacks of conventional antibiotics while yet being effective.
Journal of Advanced Zoology, 2023
Background and Introduction: The prevalence of hyperglycemic diseases known collectively as diabe... more Background and Introduction: The prevalence of hyperglycemic diseases known collectively as diabetes has reached epidemic proportions in the current century. Diabetics are particularly vulnerable to infections, which can have devastating health consequences. The purpose of this research was to examine the effects of an aqueous extract of Cyperus rotundus roots on diabetic complications in rats with diabetes caused by Alloxan. Materials and Methods: Specifically: Alloxan monohydrate, Borosilicate, and a diagnostic kit. Specifically: a diagnostic kit, a phrase, or a paraphrase. Centrifuge Micropippet, Glucose check monitoring device, electronic digital scales, EDDY's Hot plate analgesometer MK-11, and the Biofuse pico. All chemicals employed were of the AR grade variety, including the alloxan monohydrate, metformin, chloroform, diethyl ether, and ethyl ether.Results: No deaths or toxicity symptoms were observed in the AECR acute toxicity test in mice, indicating that the extract was well tolerated and the test doses were safe in the animals. The effect of AECR on fasting blood glucose level in alloxan-induced diabetic rats was measured using an auto analyzer glucose kit to determine the compound's antidiabetic activity. The plasma or blood glucose level is measured after an individual has fasted as part of a carbohydrate metabolic test. The hormone glucagon is secreted into the bloodstream during fasting to facilitate the catabolic release of glucose. Conclusion: The results show that in alloxan-induced diabetic rats, the oral administration of an aqueous extract of Cyperus rotundus exhibited neuroprotective, nephroprotective, and hepatoprotective activities by increasing insulin production and decreasing glucogan production and an SGOT and SGPT level. CC License CC-BY-NC-SA 4.0
Nigella sativa: A Potential Natural Antidote for Poisoning Cases, 2023
In several cultures, black cumin, also known as Nigella sativa, has long been used medicinally.
R... more In several cultures, black cumin, also known as Nigella sativa, has long been used medicinally.
Recent research has revealed that this plant has potent anti-inflammatory and antioxidant qualities,
making it a possible treatment for several medical conditions. Additionally, because of its capacity
to detoxify the liver and protect it from harm, Nigella sativa has demonstrated positive results as an
antidote for poisoning. Consumption of hazardous substances by accident or planned poisoning
are two prevalent causes of poisoning. The liver is the primary organ in detoxification. According
to studies, Nigella sativa can help the liver operate better and defend it from toxins’ harmful effects.
Additionally, it has been demonstrated that Nigella sativa protects against heavy metal toxicity.
Thymoquinone and thymohydroquinone, the plant’s active components, have been demonstrated
to bond with heavy metals and stop the body from absorbing them. Further studies are needed to
evaluate the efficacy and safety of Nigella sativa as an antidote for poisoning cases. Nigella sativa
presents an interesting natural alternative for treating poisoning cases, potentially complementing
traditional medical approaches. The main goal of this review is to explore the potential application
of Nigella sativa as an antidote for poisoning cases. The article discusses the plant’s strong ability to
detoxify and protect the liver. The review highlights preclinical studies that have shown promising
results but also emphasizes the need for further clinical trials to determine the efficacy and safety of
Nigella sativa as a natural alternative for treating poisoning cases.
RSC Advances, 2023
We identified twenty-two new sacubitril derivatives (5a-v) as lead compounds for various biologic... more We identified twenty-two new sacubitril derivatives (5a-v) as lead compounds for various biologically active targets. These compounds were synthesized by reacting an intermediate compound (2R,4S)-5-([1,1 ′biphenyl]-4-yl)-4-(amino)-2-methylpentanoic acid ethyl ester hydrochloride with respective carboxylic acid (RCOOH). The molecular structures of all the newly synthesized compounds were determined by 1 H and 13 C NMR, ESI mass spectrometry, FTIR spectroscopy, and CHN analysis. Moreover, compound 5n was characterized by a single-crystal X-ray diffraction (SXRD) study to confirm the structure obtained from spectral data. All these compounds were screened for various biological functions such as antifungal, antibacterial, and anti-TB activities. Among these twenty-two compounds (5a-v), some exhibited good to moderate anti-bacterial properties. Similarly, some compounds showed moderate anti-TB and antifungal activities. In addition, the anti-TB activity of compound 5q was estimated against M. tuberculosis in a nutrient starvation model (NSM). Similarly, toxicity was examined against RAW 264.7 cells. These biological activity studies were also correlated with molecular docking studies.
Journal of Coastal Life Medicine, 2022
Controlled-release systems also offer a sustained-release profile but, in contrast to sustained-r... more Controlled-release systems also offer a sustained-release profile but, in contrast to sustained-release forms, controlled-release systems are designed to lead to predictably constant plasma concentrations, independently of the biological environment of the application site. This means that they are actually controlling the drug concentration in the body, not just the release of the drug from the dosage form, as is the case in a sustainedrelease system. The main objective of this formulation development was to design an osmotic drug delivery system acting as a controlled release drug delivery system. In this formulation osmogen and release retardant were used to obtain suitable formulation. In the present study, attempts were made to formulate and evaluate API in extended release dosage form using osmotic drug delivery.
NeuroQuantology, 2022
Background: The current study assesses the anthelmintic impact of root extracts of MANSOA ALLIACE... more Background: The current study assesses the anthelmintic impact of root extracts of MANSOA ALLIACEAE on Pheretima posthuma compiled by molecular docking analysis of phytocompounds steemed from the plant with the -Tubulin (PDB ID: 1SA0). Methods: In this study, P. tomentosa root was subjected to extraction using methanol and water. In vitro, anthelmintic activity was assessed by utilizing the Pheretima posthuma and in silico molecular docking was executed making use of Autodock 4.0. Results: The outcomes revealed that the methanolic extract has the most significant dose-dependent anthelmintic activity at various doses, followed by aqueous extracts of root. Amongst all the substances, -eudesmol revealed the most effective docking rating of-6.53, which is nearer to Albendazole, i.e.,-6.79, ensuring that -eudesmol has a strong binding fondness in between protein and ligand. Conclusion: From the examinations, a conclusion can be drawn that the anthelmintic activity of P. tomentosa root in both in vitro and insilico assays. The information sustains -eudesmol to be a useful anthelmintic compound beneficial to future clinical examinations.
Research Journal of Biotechnology, 2023
Acquired immunodeficiency syndrome is caused by a retrovirus human immunodeficiency virus (HIV). ... more Acquired immunodeficiency syndrome is caused by a retrovirus human immunodeficiency virus (HIV). Resistance to antiviral drugs is alarming in antiretroviral therapy and medical management of HIV-infected patients. Thus, identifying novel antiviral drugs is critical and medicinal plants are an excellent source for such discoveries. The current study aimed to isolate and characterize the chemical constituents from leaves of Amaranthus tricolor (L) and to discover novel HIV-1 protease inhibitors. Amaranthus tricolor methanolic extract (ATME) was fractionated into chloroform and aqueous phases. The chloroform fraction was separated by column chromatography using mobile phase n-hexane: ethyl acetate (6:4 v/v). Among various fractions, SOWIS-III was purified and studied for structural interpretation. The compounds were docked with HIV-1 protease as a drug target. The structural interpretation of the SOWIS-III fraction was identified as flavanol glycoside 24methylene cycloartanol and SOWIS-P as a gallic acid derivative methyl 3,4,5-trihydroxybenzoate. The docking studies of 24-Methylenecycloartanol and HIV-1 protease showed 10 amino acid interactions with the estimated free energy of binding of-10.14 kcal/mol and the antiviral activity depicted as 36.88 nM. Methyl 3,4,5-trihydroxybenzoate and HIV-1 protease show five amino acid interactions with the estimated free energy of binding of-5.03 kcal/mol with 205.24 μM estimated antiviral activity. Hence, 24-methylene cycloartanol and methyl 3,4,5-trihydroxybenzoate can serve as drug candidates for HIV.
Research Journal of Biotechnology, 2021
Cancer is a serious concern at present. Development of affective and side effects lacking antican... more Cancer is a serious concern at present. Development of affective and side effects lacking anticancer therapy is the trending research direction in healthcare. Bioactive phytochemicals are preferred as they pretend differentially on cancer cells only without altering normal cells. The present study is aimed to isolate, characterize and to evaluate anticancer activity of a lead phytochemical from leaves of Amaranthus tricolor (L). The methanolic extract of A. tricolor (L) leaves was prepared by cold maceration and labelled as ATME. The ATME extract was fractionated with equal volume of chloroform and water. The chloroform extract was further fractionated with n-hexane:ethyl acetate (6:4 v/v) as mobile phase suggested by HPTLC. Based on bioautography the third fraction was selected for further in-silico analysis and anticancer activity. The structural interpretation of isolated compound SOWIS-III was determined as a flavonol glycoside 24methylene cycloartanol. It was docked with human oestrogen receptor and confirmed as anticancer lead molecules. The antioxidant property for 24-methylene cycloartanol was determined by DPPH method. It showed strong radical scavenging property in dose dependent manner. The IC50 values of 24-methylene cycloartanol and standard ascorbic acid was found to be 31.03 and 14.29 µg/mL respectively. The compound 24-methylene cycloartanol inhibited the growth of MCF-7 cells from human breast cancer with IC50 value 16.93 μg/mL and cisplatin with IC50 value 4.586 μg/mL determined by MTT assay. It was observed that the tested phytochemical showed promising anticancer property towards the selected cancer cell lines.
IN-VITRO ANTIBACTERIAL POTENTIAL OF ALTERNANTHERA PHILOXEROIDES (MART) GRISEB AGAINST MULTI-DRUG RESISTANT UROPATHOGENS, 2020
Antimicrobial drug resistance is one of the major threats due to the wide usage of broad-spectrum... more Antimicrobial drug resistance is one of the major threats due to the wide usage of broad-spectrum antimicrobial drugs in the general population. The present study was undertaken to investigate the antibacterial potential of Alternanthera philoxeroides methanolic extract (APME) against multidrug-resistant UTI pathogens. The preliminary phytochemical screening of the extract showed the presence of carbohydrates, amino acids, proteins, alkaloids, glycosides, steroids, flavonoids, tannins, and phenolic compounds. The quantitative estimation of total phenolic, tannin, and flavonoid contents was determined for the crude extract of APME through standard curves of gallic acid and rutin. The phenolic content of APME was found to be 12.4 mg of gallic acid equivalent (GAE)/g of extract. The tannin content was found to be 5.6 mg of GAE/g of extract, whereas the flavonoid content was found to be 3.2 mg of rutin/g of extract. The antibacterial potential of the extract was observed as maximum against Staphylococcus saprophyticus, moderate against Escherichia coli and Proteus vulgaris, and minimum against Proteus mirabilis. The minimum inhibitory concentration was ranged from 12.5 to 25 mg/ml. The Alternanthera philoxeroides were found to contain some bioactive compounds with pronounced antibacterial activity, therefore further phytochemical studies and their characterization should be needed to isolate the active constituents and evaluate the antimicrobial activities against a wide range of microbial pathogens.
WORLD JOURNAL OF PHARMACY AND PHARMACEUTICAL SCIENCES, 2019
Fermentation is a process which involves slow decomposition of organic substances, induced by enz... more Fermentation is a process which involves slow decomposition of organic substances, induced by enzymes or microorganisms that basically convert carbohydrates into organic acids. Fermented foods and beverages have a diversity of traditions and cultural preferences based on the geographical areas from which they have originated.
Journal of Pharmacognosy and Phytochemistry, 2018
Medicinal plants are important in the traditional medicine and as well as in modern pharmaceutica... more Medicinal plants are important in the traditional medicine and as well as in modern pharmaceutical drugs. Pongamia pinnata exhibits many pharmacological attributes. In traditional system of medicine various plant parts like leaves, stems, seeds and even whole parts are used for treatment. Traditionally the leaves, seeds and entire plant are used in the treatment of many diseases. Its uses includes Anti-ulcer, Anti-diarrhoeal, Anti-plasmodial, Anti-inflammatory, Anti-viral, Anti-bacterial, Anti-lice. This review encompasses the available literature on Pongamia pinnata with respect to its pharmacognostic characters, physicochemical parameters, synopsis of pharmacological activities and traditional uses. This attempt provides a direction towards further research.
International Journal of Advanced Research, 2021
We look at the clinical development of remdesivir, a prodrug that has been shown to inhibit SARS-... more We look at the clinical development of remdesivir, a prodrug that has been shown to inhibit SARS-CoV-2 replication, indicating that it could be used to treat COVID-19. Remdesivir is a nucleotide analogue prodrug that disrupts viral replication and was first tested in clinical trials in 2014 to combat the Ebola outbreak.The ability of remdesivir to inhibit coronavirus replication, including SARS-CoV-2, was later demonstrated by numerous virology laboratories. Well go over how remdesivir was discovered, how it works, and what studies are currenty being done to see how effective it is in the clinic.
Inventi Rapid: Med Chem, 2018
Benzal derivatives were synthesized by crossed aldol. Benzalacetone synthesis was done by crossed... more Benzal derivatives were synthesized by crossed aldol. Benzalacetone synthesis was done by crossed aldol condensation between benzaldehyde and acetone with 1:1 mol ratio, while dibenzalacetone in 2:1 mol ratio. Benzalacetone derivatives were synthesized by replacing benzaldehyde with its derivatives, i.e. anisaldehyde, salicylacetone, cinnamaldehyde and p- hydroxyl benzalacetone. The mono derivatives and di derivatives were synthesized by changing the concentration of benzaldehyde. The synthesized compounds were showed good antibacterial activity. The antibacterial was determined by agar well diffusion method against E.coli and Bacillus subtilis for all benzal derivatives and its was found that mono salicylalacetone exhibited highest antibacterial activity against B. subtilis and least activity was produced by p- hydroxyl benzalacetone for both E. coli and B. subtilis.
Key words: benzaldehyde, aldol condensation, benzal derivatives, antibacterial activity
Journal of Pharmacognosy and Phytochemistry, 2018
Medicinal plants are important in the traditional medicine and as well as in modern pharmaceutica... more Medicinal plants are important in the traditional medicine and as well as in modern pharmaceutical drugs. Pongamia pinnata exhibits many pharmacological attributes. In traditional system of medicine various plant parts like leaves, stems, seeds and even whole parts are used for treatment. Traditionally the leaves, seeds and entire plant are used in the treatment of many diseases. Its uses includes Anti-ulcer, Anti-diarrhoeal, Anti-plasmodial, Anti-inflammatory, Anti-viral, Anti-bacterial, Anti-lice. This review encompasses the available literature on Pongamia pinnata with respect to its pharmacognostic characters, physicochemical parameters, synopsis of pharmacological activities and traditional uses. This attempt provides a direction towards further research.
Inventi Rapid: Pharm Biotech & Microbio, 2018
The artificial generation of tissues, organs or even more complex living organisms was throughout... more The artificial generation of tissues, organs or even more complex living organisms was throughout the history of
mankind a matter of myth and dream. During the last decades this vision became feasible and has been recently introduced in
clinical medicine. Tissue engineering and regenerative medicine are terms in biomedical field that deal with the
transformations. Tissue Engineering (TE) is a scientific field mainly focused on the development of tissue and organ substitutes
by controlling biological, biophysical and/or biomechanical parameters in the laboratory. This results in elaboration of threedimensional
cellular constructs with properties more similar to natural tissues than classical monolayer cultures. These systems
enable the in-vitro study of human physiology and physiopathology more accurately, while providing a set of biomedical tools
with potential applicability in toxicology, medical devices, tissue replacement, repair and regeneration. To succeed in these
purposes, TE uses nature as an inspiration source for the generation of extracellular matrix analogues (scaffolds), either from
natural or synthetic origin as well as bioreactors and bio-devices to mimic natural physiological conditions of particular tissues.
These scaffolds embed cells in a three dimensional milieu that display signals critical for the determination of cellular fate, in
terms of proliferation, differentiation and migration, among others. The aim of this review is to analyze the state of the art of TE
and some of its application fields.
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Papers by PULIPATI SOWJANYA
liver damage caused by atorvastatin and clopidogrel. Atorvastatin and clopidogrel were delivered
intraperitoneally for 14 days at 80mg/kg and 20mg/kg respectively, while leaf extract was given orally for the
same period at 200mg/kg and 400mg/kg. To evaluate the impact of the leaf extract on the atorvastatin and
clopidogrel-induced liver damage, many biochemical markers, Serum glutamate pyruvate transaminase (SGPT),
serum glutamate oxaloacetate transaminase (SGOT), total protein, and serum alkaline phosphatase were
measured. Blood samples from mice given hydroalcoholic Calycopteris floribunda leaf extract showed a
noteworthy decrease in serum indicators, suggesting that the leaf extract had an impact on the hepatocytes' ability
to operate normally again. On the other hand, the mice receiving atorvastatin and clopidogrel treatment showed
increased levels, which suggested serious liver injury. Silymarin (100 mg/kg) was given as a benchmark. The
current investigation found that Calycopteris floribunda hydroalcoholic extract significantly protected the liver
against atorvastatin and clopidogrel-induced hepatic damage.
nilagirica (Clarke) is a member of the Asteraceae family. This plant's
leaves and flowering top have been used in traditional medicine as an
antimalarial, nerve tonic, and anticancer drug, and there is evidence of
anti-leishmanial efficacy. Material and Methods: The plant specimen
was obtained from the Ooty district in Tamil Nadu, India, during the
month of December. It was subsequently authenticated by NMKRV
College for Women, Bengaluru, Karnataka, India. The plant
specimens were stored in the herbarium of the NMKRV College for
Women, Bengaluru, Karnataka, India. Results: The morphological,
microscopic, organoleptic, and other WHO-recommended procedures
for standardization were investigated in a powdered sample of
Artemisia nilagirica (Clarke) leaf. Phytochemical investigation
revealed the existence of numerous elements such as alkaloids, amino
acids, carbohydrates, flavonoids, glycosides, terpenoids, steroids,
saponins, essential oils, tannins and phenol in diverse extracts.
Conclusion: Pharmacognostic and phytochemical analysis of
Artemisia nilagirica (Clarke) leaves would be useful for authenticating
raw material or crude medication.
Recent research has revealed that this plant has potent anti-inflammatory and antioxidant qualities,
making it a possible treatment for several medical conditions. Additionally, because of its capacity
to detoxify the liver and protect it from harm, Nigella sativa has demonstrated positive results as an
antidote for poisoning. Consumption of hazardous substances by accident or planned poisoning
are two prevalent causes of poisoning. The liver is the primary organ in detoxification. According
to studies, Nigella sativa can help the liver operate better and defend it from toxins’ harmful effects.
Additionally, it has been demonstrated that Nigella sativa protects against heavy metal toxicity.
Thymoquinone and thymohydroquinone, the plant’s active components, have been demonstrated
to bond with heavy metals and stop the body from absorbing them. Further studies are needed to
evaluate the efficacy and safety of Nigella sativa as an antidote for poisoning cases. Nigella sativa
presents an interesting natural alternative for treating poisoning cases, potentially complementing
traditional medical approaches. The main goal of this review is to explore the potential application
of Nigella sativa as an antidote for poisoning cases. The article discusses the plant’s strong ability to
detoxify and protect the liver. The review highlights preclinical studies that have shown promising
results but also emphasizes the need for further clinical trials to determine the efficacy and safety of
Nigella sativa as a natural alternative for treating poisoning cases.
Key words: benzaldehyde, aldol condensation, benzal derivatives, antibacterial activity
mankind a matter of myth and dream. During the last decades this vision became feasible and has been recently introduced in
clinical medicine. Tissue engineering and regenerative medicine are terms in biomedical field that deal with the
transformations. Tissue Engineering (TE) is a scientific field mainly focused on the development of tissue and organ substitutes
by controlling biological, biophysical and/or biomechanical parameters in the laboratory. This results in elaboration of threedimensional
cellular constructs with properties more similar to natural tissues than classical monolayer cultures. These systems
enable the in-vitro study of human physiology and physiopathology more accurately, while providing a set of biomedical tools
with potential applicability in toxicology, medical devices, tissue replacement, repair and regeneration. To succeed in these
purposes, TE uses nature as an inspiration source for the generation of extracellular matrix analogues (scaffolds), either from
natural or synthetic origin as well as bioreactors and bio-devices to mimic natural physiological conditions of particular tissues.
These scaffolds embed cells in a three dimensional milieu that display signals critical for the determination of cellular fate, in
terms of proliferation, differentiation and migration, among others. The aim of this review is to analyze the state of the art of TE
and some of its application fields.
liver damage caused by atorvastatin and clopidogrel. Atorvastatin and clopidogrel were delivered
intraperitoneally for 14 days at 80mg/kg and 20mg/kg respectively, while leaf extract was given orally for the
same period at 200mg/kg and 400mg/kg. To evaluate the impact of the leaf extract on the atorvastatin and
clopidogrel-induced liver damage, many biochemical markers, Serum glutamate pyruvate transaminase (SGPT),
serum glutamate oxaloacetate transaminase (SGOT), total protein, and serum alkaline phosphatase were
measured. Blood samples from mice given hydroalcoholic Calycopteris floribunda leaf extract showed a
noteworthy decrease in serum indicators, suggesting that the leaf extract had an impact on the hepatocytes' ability
to operate normally again. On the other hand, the mice receiving atorvastatin and clopidogrel treatment showed
increased levels, which suggested serious liver injury. Silymarin (100 mg/kg) was given as a benchmark. The
current investigation found that Calycopteris floribunda hydroalcoholic extract significantly protected the liver
against atorvastatin and clopidogrel-induced hepatic damage.
nilagirica (Clarke) is a member of the Asteraceae family. This plant's
leaves and flowering top have been used in traditional medicine as an
antimalarial, nerve tonic, and anticancer drug, and there is evidence of
anti-leishmanial efficacy. Material and Methods: The plant specimen
was obtained from the Ooty district in Tamil Nadu, India, during the
month of December. It was subsequently authenticated by NMKRV
College for Women, Bengaluru, Karnataka, India. The plant
specimens were stored in the herbarium of the NMKRV College for
Women, Bengaluru, Karnataka, India. Results: The morphological,
microscopic, organoleptic, and other WHO-recommended procedures
for standardization were investigated in a powdered sample of
Artemisia nilagirica (Clarke) leaf. Phytochemical investigation
revealed the existence of numerous elements such as alkaloids, amino
acids, carbohydrates, flavonoids, glycosides, terpenoids, steroids,
saponins, essential oils, tannins and phenol in diverse extracts.
Conclusion: Pharmacognostic and phytochemical analysis of
Artemisia nilagirica (Clarke) leaves would be useful for authenticating
raw material or crude medication.
Recent research has revealed that this plant has potent anti-inflammatory and antioxidant qualities,
making it a possible treatment for several medical conditions. Additionally, because of its capacity
to detoxify the liver and protect it from harm, Nigella sativa has demonstrated positive results as an
antidote for poisoning. Consumption of hazardous substances by accident or planned poisoning
are two prevalent causes of poisoning. The liver is the primary organ in detoxification. According
to studies, Nigella sativa can help the liver operate better and defend it from toxins’ harmful effects.
Additionally, it has been demonstrated that Nigella sativa protects against heavy metal toxicity.
Thymoquinone and thymohydroquinone, the plant’s active components, have been demonstrated
to bond with heavy metals and stop the body from absorbing them. Further studies are needed to
evaluate the efficacy and safety of Nigella sativa as an antidote for poisoning cases. Nigella sativa
presents an interesting natural alternative for treating poisoning cases, potentially complementing
traditional medical approaches. The main goal of this review is to explore the potential application
of Nigella sativa as an antidote for poisoning cases. The article discusses the plant’s strong ability to
detoxify and protect the liver. The review highlights preclinical studies that have shown promising
results but also emphasizes the need for further clinical trials to determine the efficacy and safety of
Nigella sativa as a natural alternative for treating poisoning cases.
Key words: benzaldehyde, aldol condensation, benzal derivatives, antibacterial activity
mankind a matter of myth and dream. During the last decades this vision became feasible and has been recently introduced in
clinical medicine. Tissue engineering and regenerative medicine are terms in biomedical field that deal with the
transformations. Tissue Engineering (TE) is a scientific field mainly focused on the development of tissue and organ substitutes
by controlling biological, biophysical and/or biomechanical parameters in the laboratory. This results in elaboration of threedimensional
cellular constructs with properties more similar to natural tissues than classical monolayer cultures. These systems
enable the in-vitro study of human physiology and physiopathology more accurately, while providing a set of biomedical tools
with potential applicability in toxicology, medical devices, tissue replacement, repair and regeneration. To succeed in these
purposes, TE uses nature as an inspiration source for the generation of extracellular matrix analogues (scaffolds), either from
natural or synthetic origin as well as bioreactors and bio-devices to mimic natural physiological conditions of particular tissues.
These scaffolds embed cells in a three dimensional milieu that display signals critical for the determination of cellular fate, in
terms of proliferation, differentiation and migration, among others. The aim of this review is to analyze the state of the art of TE
and some of its application fields.