Papers by Alfredo Lagares
Salud Uninorte
Introduction: Dermatophagoides farinae (DF) is a cosmopolitan mite present in the indoor house du... more Introduction: Dermatophagoides farinae (DF) is a cosmopolitan mite present in the indoor house dust. This mite is an important source of aeroallergens triggering of allergic asthma and rhinitis. The purpose of the present study was the production, and immunochemical and enzymatic characterization of allergenic extracts for use in allergologic diagnosis and therapy. Materials and methods: DF somatic extracts were prepared from pure In vitro culture of the mite from Santa Marta mite fauna, a Caribbean Colombian City. Mite proteins were extracted by osmotic lysis with 0.1 M of NH4HCO3 buffer. The soluble proteins were separated of the cellular debris by centrifugation, dialysis and filtration. Protein concentration was measured by the Bradford microassay test and the electrophoretic profile was identified by sodium dodecyl sulphate polyacrylamide gel electrophoresis (SDS-PAGE). The enzymatic activity was evaluated with the commercial Kit, Api Zym (Biomeriux ®). A Dot blot immunoassay w...
Salud Uninorte
Introduction: Dermatophagoides farinae (DF) is a cosmopolitan mite present in the indoor house du... more Introduction: Dermatophagoides farinae (DF) is a cosmopolitan mite present in the indoor house dust. This mite is an important source of aeroallergens triggering of allergic asthma and rhinitis. The purpose of the present study was the production, and immunochemical and enzymatic characterization of allergenic extracts for use in allergologic diagnosis and therapy. Materials and methods: DF somatic extracts were prepared from pure In vitro culture of the mite from Santa Marta mite fauna, a Caribbean Colombian City. Mite proteins were extracted by osmotic lysis with 0.1 M of NH4HCO3 buffer. The soluble proteins were separated of the cellular debris by centrifugation, dialysis and filtration. Proteins Protein concentration was measured by the Bradford microassay test and the electrophoretic profile was identified by sodium dodecyl sulphate polyacrylamide gel electrophoresis (SDS-PAGE). The enzymatic activity was evaluated with the commercial Kit, Api Zym (Biomeriux ®). A Dot blot immu...
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Papers by Alfredo Lagares