Papers by AMEUR AMEUR Abdelkader
In sheep, the polymorphism of major milk proteins is reduced as compared with other ruminant spec... more In sheep, the polymorphism of major milk proteins is reduced as compared with other ruminant species, like cattle and goats. As in other ruminant species, in sheep these polymorphisms were studied relative to milk quantity, quality and its manufacturing properties or for authenticity identification of dairy products. In Algeria, data concerning the characterization of the sheep breeds in the six loci codifying for major milk proteins genes are almost absent. The purpose of our study was to determine milk protein polymorphism in local sheep breed, using 30 samples of milk from local sheep breeds Hamra. Our work consisted in the isolation and characterization of milk proteins. For this, we used the precipitation of caseins pHi (pH 4.6). After extraction, purification and assay, both casein and serum protein fractions were then assayed by the Bradford method and separated by SDS-PAGE. Milk protein variants were determined by the molecular weight in gel using a molecular weight proteins ranging from 10 to 250 kDa. The electrophoretic pattern of milk samples showed the presence of four major caseins variants (αs1-, αs2-β-and k-casein) and two whey proteins (β-lactoglobulin, α-lactalbumin). Our study on percentage analysis of protein fractions of interest revealed that caseins represented 79.16% of the total protein of sheep milk, followed by whey proteins with 20.43%. According to the standards, the total ovine milk proteins showed a molecular weights, which are respectively (33, 32, 28 and 27 kDa) for caseins fraction and The β-lg, α-la fractions were determined to be around 19 and 15 kDa respectively. Further studies (Real Time qPCR) are requisite for certitude of the results on the polymorphic genes of proteins from sheep milk, in order to identify the genetic variants from the locus of each protein.
Assessment of genetic diversity of Turkish and Algerian native sheep breeds Abstract: In Algeria ... more Assessment of genetic diversity of Turkish and Algerian native sheep breeds Abstract: In Algeria and Turkey, the sheep production systems are based on the under extensive rural conditions and their genetic management has led to increased homozygosity and hence productivity loss. The identification of inter-breed and intra-breed genetic diversity plays a key role in the shaping of conservation and breeding programs. The present study was conducted to investigate the genetic diversity of native sheep breeds reared in Turkey and Algeria. A total of 240 animals from four Algerian (Hamra, Ouled Djellal, Sidaou, and Tazegzawt) and four Turkish (White Karaman, South Karaman, Karacabey Merino, and Kıvırcık) native sheep breeds were gen-otyped with fourteen microsatellite markers recommended by FAO. A total of 340 alleles were detected from fourteen markers studied. All the eight breeds exhibited moderate to high levels of genetic diversity, with a slight superiority of the Algerian sheep breeds. Overall FIS value was low, but highly significant (p < 0.001). It may have been due to the high inbreeding within the population. The mean global coefficient of gene differentiation (GST) showed that approximately 94.0 % of the genetic variation was within-population. The highest number of private alleles with a frequency above 5 % was observed in Ouled Djel-lal sheep. Structure analysis of populations studied revealed the most appropriate K with four genetic clusters. As the result, the dendrogram showed that the Algerian sheep breeds were completely separated from the Turkish sheep breeds furthermore the Bayesian clustering revealed a high level of admixture, especially in Algerian sheep populations. Pregled genetske pestrosti turških in alžirskih avtohotnih po-pulacij ovac Izvleček: Sistemi reje ovac v Alžiriji in Turčiji temeljijo na ekstenzivni kmečki reji, upravljanje s temi populacijami pa je privedlo do povečanja homozigotnosti in s tem do poslab-šanja proizvodnih lastnosti. Ocena medpasemske in znotraj-pasemske genske pestrosti igra ključno vlogo pri oblikovanju programov za ohranjanje teh pasem. Pričujoča študija je bila izvedena z namenom raziskovanja genske pestrosti avtohto-nih pasem ovac v Turčiji in v Alžiriji. Skupno 240 živali štirih alžirskih (hamra, ouled djellal, sidaou in tazegzawt) in štirih turških (white karaman, south karaman, karacabey merino in kıvırcık) pasem je bilo genotipiziranih s štirinajstimi mi-krosatelitnimi markerji, ki jih priporoča FAO. Na štirinajstih analiziranih markerskih lokusih je bilo zabeleženih 340 alelov. Vseh osem pasem je imelo zmerno do visoko stopnjo genske pestrosti z rahlo prevlado alžirskih pasem ovac. Skupna vre-dnost FIS je bila nizka, vendar statistično značilna (p < 0,001). To je verjetno posledica visokega inbridinga v populaciji. Pov-prečni globalni koeficient diferenciacije genov (GST) je poka-zal, da je približno 94,0 % genske pestrosti znotraj populacije. Največ privatnih alelov s pogostostjo nad 5 % je bilo ugoto-vljenih pri ovcah pasme ouled djellal. Analiza strukture popu-lacij je pokazala, da je K, ki predvideva štiri genetske klastre najprimernejši. Dendrogram, ki je rezultat študije, je pokazal, da so alžirske pasme ovac popolnoma ločene od turških, poleg tega pa je Bayesovo klastriranje pokazalo visoko stopnjo kri-žanja, zlasti v alžirskih populacijah ovac. Ključne besede: drobnica; avtohtone pasme ovac; gen-ska pestrost; mikrosateliti; genetske distance Acta agriculturae Slovenica, 115/1-2020 6 A. AMEUR AMEUR et al.
Exposition of variations between breeds is very important for genetic diversity. Determination of... more Exposition of variations between breeds is very important for genetic diversity. Determination of this variation is needed to reveal population structure and relationship between populations and planning national breeding and conservation programmes. This study was carried out in 296 animals from 12 different local sheep breeds (Barbarine, Ouled Djellal, Ifilene, Srandi, Darâa, Rembi, Berbere, Taâdmit, Hamra, Sidaou, Tazegzawt and D'men) reared in different regions of Algeria. Fifteen microsatellite markers were used to determine between breed genetic diversity. The population of 12 sheep breeds studied from Algeria exhibited a high number of alleles (24.67) and polymorphic information content (0.90). Observed heterozygosity values were lower than expected for all molecular markers except INRA0123 locus. Obtained G ST value from the present study indicated that 1.9% of total genetic variation resulted from the differences between the breeds. The present study supplied important information to understand between breed genetic differences. Moreover, it has provided the opportunity to discuss with previously reported results. In light of these findings, it can be said that studied microsatellite markers can be successfully used to determine genetic diversity and population structure in Algerian sheep breeds.
Exposition of variations between breeds is very important for genetic diversity. Determination of... more Exposition of variations between breeds is very important for genetic diversity. Determination of
this variation is needed to reveal population structure and relationship between populations
and planning national breeding and conservation programmes. This study was carried out in
296 animals from 12 different local sheep breeds (Barbarine, Ouled Djellal, Ifilene, Srandi, Dar^aa,
Rembi, Berbere, Ta^admit, Hamra, Sidaou, Tazegzawt and D’men) reared in different regions of
Algeria. Fifteen microsatellite markers were used to determine between breed genetic diversity.
The population of 12 sheep breeds studied from Algeria exhibited a high number of alleles
(24.67) and polymorphic information content (0.90). Observed heterozygosity values were lower
than expected for all molecular markers except INRA0123 locus. Obtained GST value from the
present study indicated that 1.9% of total genetic variation resulted from the differences
between the breeds. The present study supplied important information to understand between
breed genetic differences. Moreover, it has provided the opportunity to discuss with previously
reported results. In light of these findings, it can be said that studied microsatellite markers
can be successfully used to determine genetic diversity and population structure in Algerian
sheep breeds.
In order to study the race effect on the physico-chemical characteristics and protein profile, tw... more In order to study the race effect on the physico-chemical characteristics and protein profile, two sheep or ovine breeds Hamra and Ouled Djellal were raised in the same condition sat the ITELV (National institute of breeding) (Ain Hajar-Saida, Algeria). The analyses were focused on the pH, density, freezing, fat, total protein, dry defatted extract and total dry extract. The results obtained or these parameters showed no significant differences between the two races. Then the protein fractions were isolated and analyzed by Poly Acryl Amide Gel Electrophoresis (PAGE) under different conditions (native and in presence of urea). The resulting electrophoretic profiles have identified similarities between the two breeds milk.
Books by AMEUR AMEUR Abdelkader
Edition eldjazair, 2016
Ces dernières années, nous constatons que la science est subie une métamorphose. Ceci s'est tradu... more Ces dernières années, nous constatons que la science est subie une métamorphose. Ceci s'est traduit par la vague des ouvrages qui sont publiés dans les diverses disciplines, et ils sont présentés dans le Net, mais plus spécifiquement au niveau des bibliothèques universitaires. Ces livres sont volumineux et très compliqués, l'étudiant algérien se retrouve dans une situation très mal à gérer par ce nombre énorme des donnés dans leur recherche personnelle (exposés, mémoires et révision d'examen), se sont généralement des livres spécialisés destinent à la recherche appliquée. À la lumière de ces nouveaux éléments, nous avons l'idée de créer une série des ouvrages, appelée « Utile en Biologie » qui sera destinée aux étudiants de tronc commun de domaine de SNV (Biologie et Agronomie), cette série est caractérisée par la simplicité dans le contenu des cours et les chapitres sans l'entrer dans les détails.
Uploads
Papers by AMEUR AMEUR Abdelkader
this variation is needed to reveal population structure and relationship between populations
and planning national breeding and conservation programmes. This study was carried out in
296 animals from 12 different local sheep breeds (Barbarine, Ouled Djellal, Ifilene, Srandi, Dar^aa,
Rembi, Berbere, Ta^admit, Hamra, Sidaou, Tazegzawt and D’men) reared in different regions of
Algeria. Fifteen microsatellite markers were used to determine between breed genetic diversity.
The population of 12 sheep breeds studied from Algeria exhibited a high number of alleles
(24.67) and polymorphic information content (0.90). Observed heterozygosity values were lower
than expected for all molecular markers except INRA0123 locus. Obtained GST value from the
present study indicated that 1.9% of total genetic variation resulted from the differences
between the breeds. The present study supplied important information to understand between
breed genetic differences. Moreover, it has provided the opportunity to discuss with previously
reported results. In light of these findings, it can be said that studied microsatellite markers
can be successfully used to determine genetic diversity and population structure in Algerian
sheep breeds.
Books by AMEUR AMEUR Abdelkader
this variation is needed to reveal population structure and relationship between populations
and planning national breeding and conservation programmes. This study was carried out in
296 animals from 12 different local sheep breeds (Barbarine, Ouled Djellal, Ifilene, Srandi, Dar^aa,
Rembi, Berbere, Ta^admit, Hamra, Sidaou, Tazegzawt and D’men) reared in different regions of
Algeria. Fifteen microsatellite markers were used to determine between breed genetic diversity.
The population of 12 sheep breeds studied from Algeria exhibited a high number of alleles
(24.67) and polymorphic information content (0.90). Observed heterozygosity values were lower
than expected for all molecular markers except INRA0123 locus. Obtained GST value from the
present study indicated that 1.9% of total genetic variation resulted from the differences
between the breeds. The present study supplied important information to understand between
breed genetic differences. Moreover, it has provided the opportunity to discuss with previously
reported results. In light of these findings, it can be said that studied microsatellite markers
can be successfully used to determine genetic diversity and population structure in Algerian
sheep breeds.