Papers by José Vicente Tagliarini
Langenbeck's Archives of Surgery, Jan 8, 2024
Annales françaises d'Oto-rhino-laryngologie et de Pathologie Cervico-faciale, Oct 1, 2013
American Journal of Clinical Pathology, Aug 1, 2006
Laryngeal carcinomas are aggressive neoplasms with controversial association with the human papil... more Laryngeal carcinomas are aggressive neoplasms with controversial association with the human papillomavirus (HPV) and Epstein-Barr virus (EBV). So far, the impairment of p53 protein function and its impact on cellular proliferation has not been studied adequately in these tumors. In this work, molecular biologic techniques were used to assess the frequency of HPV and EBV in 110 squamous cell carcinomas of the larynx. In addition, accumulation of p53 and Ki-67 cell proliferation antigen expression in malignant cells was assessed by immunohistochemical analysis. High-grade HPV was found in 37.3% of cases, and none had demonstrable EBV infection. Accumulation of p53 was found in 78.2% of the cases, and it was related to a high Ki-67 labeling index and higher histologic grade. The results demonstrate association of HPV with more than one third of laryngeal carcinomas studied, mainly glottic tumors. Tumors with increased cell proliferation were more frequently high grade, with p53 accumulation and lymph node metastasis. Head and neck cancers, which include tumors from the oral cavity, pharynx, paranasal sinus, and larynx, have a high prevalence and complex pathogenesis. 1 In this setting, laryngeal malignant tumors deserve special attention among all head and neck cancers. According to the International Agency for Research on Cancer and World Health Organization, 161,403 new cases of laryngeal cancer were reported worldwide in 2000. Laryngeal cancer represents 1.5% of all new cases of cancer diagnosed yearly and 30% and 12% of all new cases of head and neck tumors in males and females, respectively. These tumors are responsible for high mortality rates (1.4% of all deaths due to cancer each year) and high morbidity. 2 Histologically, more than 95% of all laryngeal cancers are squamous cell carcinomas (SCCs). Although it is well known that tobacco and alcohol abuse are major risk factors for the development of laryngeal SCC, 3 infectious agents also may be implicated in some cases. Human papillomavirus (HPV) is the most frequently studied virus that seems to be associated with laryngeal SCC, but some authors claim that Epstein-Barr virus (EBV) also might have a role in the pathogenesis of these tumors. 4 In addition, there are conflicting data in the literature about the importance of some putative prognostic markers in laryngeal SCC, such as cell proliferation markers and expression of the product of the TP53 tumor suppressor gene, the p53 protein. The aims of the present study were to assess the frequency of HPV infection, EBV infection, p53 accumulation, and Ki-67 cell proliferation antigen in SCC of the larynx and to study a possible association of each with clinicopathologic data.
Revista Brasileira De Oftalmologia, Dec 1, 2007
Departamento de Oftalmologia Otorrinolaringologia e Cirurgia de Cabeca e Pescoco da Faculdade de ... more Departamento de Oftalmologia Otorrinolaringologia e Cirurgia de Cabeca e Pescoco da Faculdade de Medicina Universidade Estadual Paulista Julio Mesquita Filho - UNESP, Botucatu (SP)
Revista Brasileira De Oftalmologia, Dec 1, 2006
Departamento de OFT/ORL/CCP Faculdade de Medicina de Botucatu Universidade Estadual Paulista Juli... more Departamento de OFT/ORL/CCP Faculdade de Medicina de Botucatu Universidade Estadual Paulista Julio Mesquita Filho - UNESP, Botucatu, SP
Brazilian Journal of Otorhinolaryngology, Mar 1, 2005
Medical Case Studies, Jun 30, 2011
Endocrine Connections
Background Thyroid nodules diagnosed as 'atypia of undetermined significance/follicular lesio... more Background Thyroid nodules diagnosed as 'atypia of undetermined significance/follicular lesion of undetermined significance' (AUS/FLUS) or 'follicular neoplasm/suspected follicular neoplasm' (FN/SFN), according to Bethesda’s classification, represent a challenge in clinical practice. Computerized analysis of nuclear images (CANI) could be a useful tool for these cases. Our aim was to evaluate the ability of CANI to correctly classify AUS/FLUS and FN/SFN thyroid nodules for malignancy. Methods We studied 101 nodules cytologically classified as AUS/FLUS (n = 68) or FN/SFN (n = 33) from 97 thyroidectomy patients. Slides with cytological material were submitted for manual selection and analysis of the follicular cell nuclei for morphometric and texture parameters using ImageJ software. The histologically benign and malignant lesions were compared for such parameters which were then evaluated for the capacity to predict malignancy using the classification and regression t...
Archives of Endocrinology and Metabolism, Jan 17, 2023
Objective: Our aim was to assess the ability of serum magnesium (Mg), measured on the first posto... more Objective: Our aim was to assess the ability of serum magnesium (Mg), measured on the first postoperative day (Mg1PO), to predict the need for calcium (Ca) replacement in patients undergoing total thyroidectomy (TT). Subjects and methods: Eighty patients undergoing TT, with Mg1PO and PTH dosage in the first (PTH1h) and eighth (PTH8h) hours after TT, were evaluated for the need for Ca replacement. Data were evaluated by uni/multivariate logistic regression and Receiver Operating Characteristic (ROC) curve. Results: 32 patients (40%) required Ca replacement. Median PTH1h, PTH8h and Mg1PO were higher in the no replacement group: 17 versus (vs) 3 pg/mL (p < 0.001), 18.2 vs 3.0 pg/mL (p < 0.001) and 2 vs 1.6 mg/dL (p < 0.001), respectively. Mg1PO was the isolated predictor for this replacement (odds ratio = 0.0004, 95% confidence interval: 0.000003-0.04; p = 0.001), with the cutoff value of 1.8 mg/dL showing sensitivity and specificity of 78.1% and 87.5%, respectively. Conclusions: In this group of patients, serum Mg1PO was the isolated predictor for the need for Ca replacement.
Archives of Endocrinology and Metabolism
Objective: Cytological analysis and Bethesda classification of thyroid nodules is the standard me... more Objective: Cytological analysis and Bethesda classification of thyroid nodules is the standard method of diagnosing differentiated thyroid carcinoma (DTC). However, even for nodules with a non-malignant cytological diagnosis, there is a not insignificant risk of cancer. There are doubts whether this lack of certainty would influence patient prognosis. Our aim was to compare patients with DTC, classified according to the preoperative cytological diagnosis, regarding their evolution. Subjects and methods: A retrospective study was carried out with 108 DTC patients submitted to total thyroidectomy (TT) between 2009 and 2015, divided into three groups according to preoperative cytological diagnosis (Bethesda classification): classes I/II, III/IV, and V/VI. Groups were compared for evolution considering response to treatment at last evaluation as well as time disease free. Statistical analysis used ANOVA, chi squared, and Kaplan-Meier curves with p<0.05 considered significant. Results: Groups differed for time between nodule puncture and TT [in months; V/VI (2.35 ± 2.48) < III/IV (7.32 ± 6.34) < I/II (13.36 ± 8.9); p < 0.0001]. There was no significant difference between groups for evolution at final evaluation (disease free status; classes I/II: 71.4%; classes III/IV: 60%; classes V/VI: 66.6%; p = 0.7433), as well as time disease free (in months; classes I/II: 34.57 ± 25.82; classes III/IV: 38.04 ± 26.66; classes V/VI: 30.84 ± 26.34; p = 0.3841). Conclusions: DTC patients classified according to preoperative cytological diagnosis did not differ for evolution. Although patients with non-malignant cytological diagnoses were submitted to TT later, this did not affect the evolution of the cases.
Revista Brasileira De Otorrinolaringologia, 2000
Revista Brasileira De Otorrinolaringologia, 2021
Revista Brasileira De Oftalmologia, 2006
Departamento de OFT/ORL/CCP Faculdade de Medicina de Botucatu Universidade Estadual Paulista Juli... more Departamento de OFT/ORL/CCP Faculdade de Medicina de Botucatu Universidade Estadual Paulista Julio Mesquita Filho - UNESP, Botucatu, SP
Paediatric respiratory physiology and sleep, 2018
Introduction: Obstructive sleep apnea syndrome (OSA) in children is related to hypertrophy of the... more Introduction: Obstructive sleep apnea syndrome (OSA) in children is related to hypertrophy of the tonsils, adenotonsillectomy being the treatment of choice. However, perioperative risks are not well established, assessment of routine preoperative polysomnography not feasible. The objective of the study was to evaluate the economic and technical viability (frequency of failure) of unsupervised polysomnography in children before adenotonsillectomy. Methods: The prospective study was approved by the local research ethics committee. 146 children, 57 male, aged 3 to 11 years, with indication of adenotonsillectomy, were invited to preoperative polygraphic monitoring. We analyzed the frequency of failed exams, failures per sensor, its correlation to age of the children and compared costs to standard full-night polysomnography. Results: General failure rate was 28.08% (n = 41), with no difference between genders (χ2=0.0644 p=0.7997), but a greater risk for preschool children (RR =1.2386 (CI 95%: = 0.724 to 2.118). Failure of oximetry was observed in 14.3%, nasal cannula in 10.2%, combination of both in 4.1%., thoracoabdominal belt in none. Costs of the unsupervised PSG was estimated to be 63% of the standard PSG, even if including the high failure rate being more cost saving. Conclusion: Unsupervised polysomnography was technically and economically feasible, its installation to be performed by trained professionals to avoid failure of sensors, mostly oximetry.
Brazilian Journal of Otorhinolaryngology, 2017
Introduction: Dysphonia is a common symptom after thyroidectomy. Objective: To analyze the vocal ... more Introduction: Dysphonia is a common symptom after thyroidectomy. Objective: To analyze the vocal symptoms, auditory-perceptual and acoustic vocal, videolaryngoscopy, the surgical procedures and histopathological findings in patients undergoing thyroidectomy. Methods: Prospective study. Patients submitted to thyroidectomy were evaluated as follows: anamnesis, laryngoscopy, and acoustic vocal assessments. Moments: pre-operative, 1st post (15 days), 2nd post (1 month), 3rd post (3 months), and 4th post (6 months). Results: Among the 151 patients (130 women; 21 men). Type of surgery: lobectomy + isthmectomy n = 40, total thyroidectomy n = 88, thyroidectomy + lymph node dissection n = 23. Vocal symptoms were reported by 42 patients in the 1st post (27.8%) decreasing to 7.2% after 6 months. In the acoustic analysis, f0 and APQ were decreased in women. Videolaryngoscopies showed that 144 patients (95.3%) had normal exams in the preoperative moment. Vocal fold palsies were diagnosed in 34 paralyzes at the 1st post, 32 recurrent laryngeal nerve (lobectomy + isthmectomy n = 6; total thyroidectomy n = 17; thyroidectomy + lymph node dissection n = 9) and 2 superior laryngeal nerve (lobectomy + isthmectomy n = 1; Total thyroidectomy + lymph node dissection n = 1). After 6 months, 10 patients persisted with paralysis of the recurrent laryngeal nerve (6.6%). Histopathology and correlation with vocal fold palsy: colloid nodular goiter (n = 76; palsy n = 13), thyroiditis (n = 8; palsy n = 0), and carcinoma (n = 67; palsy n = 21).
Endocrine Research, 2016
Stimulated thyroglobulin (STg) levels in patients with differentiated thyroid carcinomas (DTCs) a... more Stimulated thyroglobulin (STg) levels in patients with differentiated thyroid carcinomas (DTCs) after total thyroidectomy (TT) and before radioactive iodine ((131)I) ablation/therapy (RIT) are predictive of therapeutic success but can be influenced by the thyroid-stimulating hormone (TSH) level. This study compared the reliability of the STg/TSH ratio and STg measurement in predicting the success of RIT. Sixty-three DTC patients submitted to TT were assessed retrospectively to compare the ability of STg level and the STg/TSH ratio to predict successful RIT. In this study 48 (76.2%) patients had successful RIT. The successful and unsuccessful groups received different (131)I doses and had different STg levels and STg/TSH ratios. The STg and STg/TSH ratio cutoff values that predicted successful RIT were 4.41 ng/mL (sensitivity of 86.7% and specificity of 77%) and 0.093 (sensitivity of 80% and specificity of 79.2%), respectively. Age, STg level, STg/TSH ratio, and (131)I dose were associated with successful RIT, but after multivariate analysis only STg remained associated (p &amp;amp;amp;amp;lt; 0.05). In conclusion, our data suggest that the STg/TSH ratio and measurement of STg are equally reliable in predicting successful RIT in DTC patients.
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Papers by José Vicente Tagliarini