Papers by Eduardo Marcandalli Boleta
Protoplasma, 2009
Exposure of the filamentous turf green alga Urospora penicilliformis to ambient and artificial ul... more Exposure of the filamentous turf green alga Urospora penicilliformis to ambient and artificial ultraviolet radiation (UVR) revealed a considerable resilient species. This explains the ability of this alga to thrive in the middleupper intertidal zones of the Arctic sea where it is periodically exposed to environmental extremes. A transient UVR effect on photosynthesis under photosynthetically active radiation (PAR) + UV-A and PAR + UV-A + UV-B was found, but dynamic recovery of photoinhibition was observed immediately after reduction of the photon fluence rate of PAR in the absence or presence of background UVR under laboratory and natural solar radiation, respectively. Chlorophylls, carotenoids, and xanthophyll cycle pigments (violaxanthin, antheraxanthin, and zeaxanthin) concentrations were not significantly different between freshly collected samples and filaments exposed to additional laboratory radiation treatment. The ultrastructure of the U. penicilliformis gametophytes showed that the cells are well adapted to UVR. No significant ultrastructural alterations were observed in filaments exposed to different spectral irradiance in the laboratory compared to in situ acclimated specimen. The antioxidant α-tocopherol was detected in minute quantity while the search for flavonoid-like compounds was negative. Other UV screening strategies or certain genetically fixed physiological protective mechanism could be operating in this species responsible for their occurrence in higher shoreline and ecological success. Further molecular and biochemical studies are needed to elucidate the stress resistance in this turf alga. There is an indication that the extremely thick cell wall of U. penicilliformis gametophytes covered with mucilage sheath and dense layer of mineral depositions may provide a shield against unfavorable environmental conditions in general and against UVR in particular.
Nitrogen in Agriculture - Updates, Feb 1, 2018
The biological nitrogen fixation (BNF) process in wheat (Triticum aestivum L.) occurs by diazotro... more The biological nitrogen fixation (BNF) process in wheat (Triticum aestivum L.) occurs by diazotrophic bacteria, particularly Azospirillum brasilense. However, researches are lacking on BNF efficiency to define how much mineral nitrogen (N) can be applied to achieve more sustainable high yields, and if urea with the urease enzyme inhibitor is less harmful, benefiting BNF in grasses (cereals). Therefore, the objective was to evaluate the effect of N sources (urea and Super N, urea with urease enzyme inhibitor N-(n-butyl thiophosphoric triamide) (NBPT) and N rates (0, 50, 100, 150, and 200 kg ha −1) applied in topdressing associated with inoculation with A. brasilense, regarding the leaf N concentration, leaf chlorophyll index (LCI), accumulation of N in the straw and grains, the nitrogen utilization efficiency (NUE), recovery of the applied nitrogen (RAN), physiological efficiency (FE), agronomic efficiency (AE), and wheat grain yield in the Brazilian Cerrado (tropical savanna) region. The N sources provide similar N accumulations in straw and grains, and wheat grain yield. Inoculation with A. brasilense afforded higher N grain concentration (increase in protein content more sustainably) by applying less N fertilizer in topdressing. Inoculation with A. brasilense increased the AE, RAN, and NUE.
Advances in Legumes for Sustainable Intensification
Journal of Crop Science and Biotechnology
The rhizobacteria of genus Azospirillum and Bacillus are effective at fixing atmospheric nitrogen... more The rhizobacteria of genus Azospirillum and Bacillus are effective at fixing atmospheric nitrogen, promote plant growth and can largely contribute to beneficial recovery from degraded pastures. This study aimed to evaluate the development and nutritional status of Urochloa brizantha cv. Marandu in response to nitrogen doses along with inoculation of Azospirillum brasilense and Bacillus subtilis. The experiment was carried in a completely randomized design in a greenhouse with four replications and in factorial scheme 4 × 5. The treatments were consisted of seeds inoculation (without inoculation, A. brasilense (strains Ab-V5, Ab-V6), B. subtilis (strain CCTB04) and co-inoculation with strains of A. brasilense + strain of B. subtilis, and nitrogen doses (0, 50, 100, 150 and 200 kg ha−1, equivalent to 0, 25, 50, 75 and 100 mg dm−3 N) from urea. The sole inoculation of A. brasilense and B. subitilis promoted plant height of Marandu grass along with nitrogen fertilization. The co-inoculation of these bacteria increased potassium accumulation in Marandu grass along with nitrogen doses. Regardless of bacterial inoculation, number of tillers, leaves, dry matter accumulation, crude protein and accumulations of N, P, Ca, S and Mg were increased with increasing nitrogen doses in the first forage cycle.
European Journal of Agronomy, 2022
New studies are needed to optimize the nitrogen (N) amount that can be applied to utilize the <... more New studies are needed to optimize the nitrogen (N) amount that can be applied to utilize the <i>Azospirillum brasilense</i> benefits. In addition, information regarding the interaction between the urease inhibitor and biological nitrogen fixation (BNF) and how they affect the macronutrients accumulation are also needed. We evaluate the effect of N sources and doses associated with <i>A. brasilense</i> regarding the macronutrients accumulation in straw and grains and wheat grain yield in tropical conditions. A randomized block experimental design was used with four replications in a 2<b> </b>×<b> </b>5 × 2 factorial arrangement as follows: two N sources (urea and urea with urease enzyme inhibitor NBPT; five N doses (0, 50, 100, 150, and 200<b> </b>kg ha<sup>−1</sup>) applied in topdressing; with and without <i>A. brasilense</i> inoculation. We found that an increase in N doses positively influenced t...
Open Agriculture, 2020
Information regarding the interaction between biological nitrogen fixation (BNF) with Azospirillu... more Information regarding the interaction between biological nitrogen fixation (BNF) with Azospirillum brasilense inoculation and the use of silicon (Si) is needed. Silicon exerts numerous benefits on grasses, especially when the plants are subjected to biotic and abiotic stresses, affecting plant nutrition. The aim of this research was to determine if there is a synergistic effect between the inoculation with A. brasilense and Si use, on macro-nutrient content in corn shoot and root. The field trial was performed in Selvíria, Brazil, on a Typic Rhodic Hapludox soil under no-till system. The experimental design was a completely randomized block design with four replicates arranged in a 2 × 5 × 2 triple factorial arrangement, consisting of two soil acidity corrective sources (dolomitic limestone and Ca and Mg silicate as sources of Si); five N doses (0, 50, 100, 150 and 200 kg ha-1 applied in topdressing); with and without seed inoculation with A. brasilense. The inoculation favored N co...
Revista Brasileira de Engenharia Agrícola e Ambiental, 2018
In search of a more sustainable agriculture, the use of beneficial microorganisms has been highli... more In search of a more sustainable agriculture, the use of beneficial microorganisms has been highlighted, because they are low-cost and can reduce the use of fertilizers and increase grain yield. The present study aimed to evaluate the efficiency of A. brasilense inoculation and the best form and dose of inoculation in maize, measuring the impact on some physical characteristics and on its nutrition. The experiment was conducted in a greenhouse, in Ilha Solteira, SP, Brazil, in a completely randomized design, with four replicates and eight treatments: 1) control; 2) Seed 1x; 3) Seed 2x; 4) Soil 1x; 5) Soil 2x; 6) Leaf 1x; 7) Leaf 2x; 8) Seed 1x + Leaf 1x, respectively representing in each treatment the site and dose of application (1x, dose recommended by the manufacturer; 2x, twice the dose recommended by the manufacturer). No differences were found in any physical characteristics evaluated between treatments; however, for nutrient contents in the leaf tissue, there was effect on Zn ...
Revista Brasileira de Engenharia Agrícola e Ambiental, 2018
Boron is one of the most limiting micronutrients in the grain production system in Brazil. Thus, ... more Boron is one of the most limiting micronutrients in the grain production system in Brazil. Thus, the objective of this study was to evaluate the effects of the application forms and doses of boron on the concentration of this nutrient in the leaves, straw, and grains of wheat, as well as the production components and grain yield. The residual effect of boron in the soil was also evaluated. An experiment was conducted in a no-tillage system in Oxisol with clay texture in Selvíria, Mato Grosso do Sul. The experimental design was a randomized block design with four replicates arranged in a 4 x 3 factorial scheme. Four doses of boron (0, 1, 2, and 4 kg ha-1) were applied with a boric acid source (B = 17%) and three application forms: a) during the desiccation of the predecessor straw with herbicide; b) at the time of sowing in soil along with formulated fertilization seeding, and c) via the leaves with the application of post-emergent herbicide. The application of boron to the soil at t...
Journal of Agricultural Science, 2018
There is a lack of more comprehensive research defining how much mineral N can be applied as rega... more There is a lack of more comprehensive research defining how much mineral N can be applied as regards the efficiency of BNF to attain high with Azospirillum brasilense, what is a bacterium that stands out for its biological nitrogen fixation (BNF) in corn crops, reaching economically sustainable yields. Moreover, it would be interesting to investigate the use of silicon, which exerts numerous benefits on grasses, especially when the plants are subjected to biotic and abiotic stresses. The aim of this work was to study a synergistic effect between the inoculation with A. brasilense and the application of silicon, thus enabling a higher efficiency of nitrogen fertilization, evaluating the grain yield of irrigated corn in economic terms. The experiment was conducted in Selvíria, MS, Brazil, under a no-till system, on a Latossolo Vermelho distrófico (Oxisol). The experiment was set up as a randomized block design with four replications, in a 2 × 5 × 2 factorial arrangement consisting of ...
Journal of Agricultural Science, 2018
Boron is one of the most limiting micronutrients in grains production system in Brazil. In this w... more Boron is one of the most limiting micronutrients in grains production system in Brazil. In this way, the objective was to evaluate the effect of forms of application and doses of boron in irrigated wheat grain yield evaluating the economic terms in Cerrado region. The experiment was conducted in no-tillage system in an Oxisol with clay texture in Selvíria, MS, Brazil. The experimental design was a randomized block design with four replicates, arranged in a 4 × 3 factorial scheme: four doses of boron (0, 1, 2 and 4 kg ha-1) with boric acid source (B = 17%); and three apllication forms: a) in desiccation of the predecessor straw, together with herbicide; b) at the time of sowing, in soil along with the formulated fertilization seeding and c) via leaf tissue with the application of post emergent herbicide. The application of 2 kg ha-1 provides greater grain yields, but the highest economic return was obtained at the dose of 1 kg ha-1, with application in soil, ensuring profitability fr...
Open Agriculture, 2018
The aim of this study was to analyze whether there are differences between the inoculation with A... more The aim of this study was to analyze whether there are differences between the inoculation with Azospirillum brasilense and the silicon application, thus enabling a higher efficiency of nitrogen fertilization, evaluating micronutrients and silicon concentration in shoots and roots of irrigated corn (Zea mays). The experiment was conducted in Selviria, Brazil, under a no-till system, on a Typic Rhodic Hapludox. The experiment was set up as a randomized block design with four replications, in a 2 × 5 × 2 factorial arrangement consisting of two soil corrective sources (dolomitic limestone and Ca and Mg silicate as source of Si); five N rates (0, 50, 100, 150 and 200 kg ha-1); with and without inoculation with A. brasilense. N rates increased B, Cu and Fe concentrations in shoots and B, Cu, Fe, Mn, Zn and Si in roots. Inoculation provided greater concentrations of B and Fe in shoots, and B in roots. Although inoculation with A. brasilense favored micronutrient uptake, it negatively affe...
Frontiers in Environmental Science
Sustainable intensification of cropping systems requires to increase productivity and nutrients u... more Sustainable intensification of cropping systems requires to increase productivity and nutrients use efficiency while reducing negative impacts of agricultural management practices on ecosystem and environment. Plant growth-promoting rhizobacteria (PGPR) inoculations are considered one of the most promising and safe strategy to alleviate environmental alterations in context of climatic extremes to improve plant nutrition while reducing dependency of nitrogen (N) fertilizer application. This study investigated the interactive effects of N levels and inoculation with A. brasilense on plant biomass, grain yield, agronomic efficiency (AE) of applied N, apparent N-fertilizer recovery (AFR) and N content in plant targeting economic feasibility of wheat production system. The field trial tested 4 N application levels applied in side-dressing (control, low, average and high; named 0, 50, 100 and 200 kg N ha−1) and two inoculations (without and with A. brasilense seed inoculation). The result...
Agronomy
Enrichment of staple food with zinc (Zn) along with solubilizing bacteria is a sustainable and pr... more Enrichment of staple food with zinc (Zn) along with solubilizing bacteria is a sustainable and practical approach to overcome Zn malnutrition in human beings by improving plant nutrition, nutrient use efficiency, and productivity. Common bean (Phaseolus vulgaris L.) is one of a staple food of global population and has a prospective role in agronomic Zn biofortification. In this context, we evaluated the effect of diazotrophic bacterial co-inoculations (No inoculation, Rhizobium tropici, R. tropici + Azospirillum brasilense, R. tropici + Bacillus subtilis, R. tropici + Pseudomonas fluorescens, R. tropici + A. brasilense + B. subtilis, and R. tropici + A. brasilense + P. fluorescens) in association with soil Zn application (without and with 8 kg Zn ha−1) on Zn nutrition, growth, yield, and Zn use efficiencies in common bean in the 2019 and 2020 crop seasons. Soil Zn application in combination with R. tropici + B. subtilis improved Zn accumulation in shoot and grains with greater shoot...
Frontiers in Sustainable Food Systems
Agricultural practices that allow a productive increase in a sustainable manner are becoming incr... more Agricultural practices that allow a productive increase in a sustainable manner are becoming increasingly necessary to feed an ever-growing global population. The inoculation with Azospirillum brasilense has the potential to reduce the use of synthetic mineral fertilizers with efficient capacity to promote plant growth and increase nutrition. Therefore, this research was developed to investigate the potential use of A. brasilense to increase the accumulation of macro- and micronutrients and its influence on grain yield, plant height, and spike population in two wheat cultivars (CD1104 and CD150), under irrigated conditions in the Brazilian Cerrado. The study was carried out in a Rhodic Hapludox under a no-tillage system. The experiment was designed in randomized blocks with six replications, arranged in a 2 × 2 factorial scheme: two cultivars (CD150 and CD1104) and two levels of inoculation (control and with A. brasilense). The inoculation with A. brasilense provided greater accumul...
Plants
Sustainable management strategies are needed to improve agronomic efficiency and cereal yield pro... more Sustainable management strategies are needed to improve agronomic efficiency and cereal yield production under harsh abiotic climatic conditions such as in tropical Savannah. Under these environments, field-grown crops are usually exposed to drought and high temperature conditions. Silicon (Si) application could be a useful and sustainable strategy to enhance agronomic N use efficiency, leading to better cereal development. This study was developed to explore the effect of Si application as a soil amendment source (Ca and Mg silicate) associated with N levels applied in a side-dressing (control, low, medium and high N levels) on maize and wheat development, N uptake, agronomic efficiency and grain yield. The field experiments were carried out during four cropping seasons, using two soil amendment sources (Ca and Mg silicate and dolomitic limestone) and four N levels (0, 50, 100 and 200 kg N ha−1). The following evaluations were performed in maize and wheat crops: the shoots and root...
Scientific Reports
Marcelo carvalho Minhoto teixeira filho * this research was developed to investigate whether inoc... more Marcelo carvalho Minhoto teixeira filho * this research was developed to investigate whether inoculation with Azospirillum brasilense in combination with silicon (Si) can enhance N use efficiency (NUE) in wheat and to evaluate and correlate nutritional and productive components and wheat grain yield. The study was carried out on a Rhodic Hapludox under a no-till system with a completely randomized block design with four replications in a 2 × 2 × 5 factorial scheme: two liming sources (with Ca and Mg silicate as the Si source and limestone); two inoculations (control-without inoculation and seed inoculation with A. brasilense) and five sidedress N rates (0, 50, 100, 150 and 200 kg ha −1). The results of this study showed positive improvements in wheat growth production parameters, NUE and grain yield as a function of inoculation associated with N rates. Inoculation can complement and optimize N fertilization, even with high N application rates. The potential benefits of Si use were less evident; however, the use of Si can favour N absorption, even when associated with A. brasilense. Therefore, studies conducted under tropical conditions with Ca and Mg silicate are necessary to better understand the role of Si applied alone or in combination with growth-promoting bacteria such as A. brasilense. Wheat (Triticum aestivum L.) is a grain crop that has significant economic importance among cool season cereals due to its high grain-yield capacity 1,2. In addition to maize (Zea mays L.) and rice (Oryza sativa L.), wheat is responsible for above 50% of human calories and is an important food source in regions with rapid population growth, such as Africa, Asia and Latin America 3. However, in the last five years, 51.2% of Brazil's wheat consumption was imported from Argentina, the United States, Russia and Paraguay 4. Similar to other grain crops, wheat production under savanna conditions is limited by nitrogen (N) availability 5. In addition, it has been estimated that as much as 17% of the total operating profit of the wheat being produced on the Brazilian savanna is due to side-dress N application 6. The production and over application of N fertilizer contribute to a series of environmental problems, such as air pollution, greenhouse gas emissions, global warming, contamination of water bodies and eutrophication 7-9. Therefore, integrated N management strategies could contribute significantly to improving N use efficiency (NUE) under savanna conditions, particularly for wheat, which has high N requirements 10,11. Bacteria with multiple plant-growth promoting traits (PGPB, plant-growth promoting bacteria) can improve NUE and increase the growth and grain yields of cereal crops under tropical conditions 1,12-14. Numerous PGPB genera show positive relations with different plant species (for example, Azospirillum, Rhizobium, Bradyrhizobium, Bacillus and Pseudomonas) 15. The genus Azospirillum is currently one of the most commercially employed PGPB 16,17. This microorganism can colonize several plant species and significantly improves their growth, development and yield under field conditions 16,18. Previous studies with Azospirillum highlight its capacity to fix N 2 , as well as its benefits in terms of promoting plant growth via the synthesis of phytohormones,
Semina: Ciências Agrárias
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Papers by Eduardo Marcandalli Boleta