Papers by Vincent Rotello
Advanced Functional Materials, 2006
Highly fluorinated comb-shaped copolymers as proton exchange membranes (PEMs): improving PEM prop... more Highly fluorinated comb-shaped copolymers as proton exchange membranes (PEMs): improving PEM properties through rational design Norsten, T. B.; Guiver, Michael; Murphy, J.; Astill, Toby; Navessin, Titichai; Holdcroft, Steven; Frankamp, B.; Rotello, V.; Ding, Jianfu Contact us / Contactez nous: [email protected]. http://nparc.cisti-icist.nrc-cnrc.gc.ca/npsi/jsp/nparc_cp.jsp?lang=fr L'accès à ce site Web et l'utilisation de son contenu sont assujettis aux conditions présentées dans le site Web page / page Web http://dx.
ACS Macro Letters, 2012
ABSTRACT
Abstract This paper describes the creation of hybrid surfaces containing cationic nanoparticles a... more Abstract This paper describes the creation of hybrid surfaces containing cationic nanoparticles and biocompatible PEG (polyethylene glycol) brushes that manipulate bacterial adhesion for potential diagnostic and implant applications.
Soft Matter, 2009
ABSTRACT
Angewandte Chemie (International ed. in English), Jan 14, 2015
A nanoparticle design featuring pH-responsive alkoxyphenyl acylsulfonamide ligands is reported he... more A nanoparticle design featuring pH-responsive alkoxyphenyl acylsulfonamide ligands is reported herein. As a result of ligand structure, this nanoparticle is neutral at pH 7.4, becoming positively charged at tumor pH (<6.5). The particle uptake and cytotoxicity increase over this pH range. This pH-controlled uptake and toxicity makes this particle a promising tool for tumor selective therapy.
Bioconjugate chemistry, Jan 17, 2015
We describe a method for quantitative monitoring of subcellular protein trafficking using nanopar... more We describe a method for quantitative monitoring of subcellular protein trafficking using nanoparticle-stabilized nanocapsules for protein delivery. This method provides rapid delivery of the protein into the cytosol, eliminating complications from protein homeostasis processes found with cellularly expressed proteins. After delivery, nuclear protein trafficking was followed by real time microscopic imaging. Quantitative analyses of the accumulation percentage and the import dynamics of the nuclear protein trafficking, demonstrate the utility of this method for studying intracellular trafficking systems.
Tetrahedron letters, Jan 3, 2015
Host-guest interactions between a synthetic receptor, cucurbit[7]uril (CB[7]), and gold nanoparti... more Host-guest interactions between a synthetic receptor, cucurbit[7]uril (CB[7]), and gold nanoparticles (AuNPs) have been quantified using isothermal titration calorimetry. AuNPs were functionalized with ligands containing tertiary or quaternary benzylamine derivatives, with electron donating or withdrawing groups at the para position of the benzene ring. Analysis of binding interactions reveals that functional groups at the para position have no significant effect on binding constant. However, headgroups bearing a permanent positive charge increased the binding of AuNPs to CB[7] ten-fold compared to monomethyl counterparts.
Journal of the American Chemical Society, Jan 26, 2015
Optical spectroscopic methods do not routinely provide information on higher order hierarchical s... more Optical spectroscopic methods do not routinely provide information on higher order hierarchical structure (tertiary/quaternary) of biological macromolecules and assemblies. This necessitates the use of time-consuming and material intensive techniques, such as protein crystallography, NMR, and electron microscopy. Here we demonstrate a spectroscopic phenomenon, superchiral polarimetry, which can rapidly characterize ligand-induced changes in protein higher order (tertiary/quaternary) structure at the picogram level, which is undetectable using conventional CD spectroscopy. This is achieved by utilizing the enhanced sensitivity of superchiral evanescent fields to mesoscale chiral structure.
Nature chemistry, Jan 23, 2015
Bioorthogonal catalysis broadens the functional possibilities of intracellular chemistry. Effecti... more Bioorthogonal catalysis broadens the functional possibilities of intracellular chemistry. Effective delivery and regulation of synthetic catalytic systems in cells are challenging due to the complex intracellular environment and catalyst instability. Here, we report the fabrication of protein-sized bioorthogonal nanozymes through the encapsulation of hydrophobic transition metal catalysts into the monolayer of water-soluble gold nanoparticles. The activity of these catalysts can be reversibly controlled by binding a supramolecular cucurbit[7]uril 'gate-keeper' onto the monolayer surface, providing a biomimetic control mechanism that mimics the allosteric regulation of enzymes. The potential of this gated nanozyme for use in imaging and therapeutic applications was demonstrated through triggered cleavage of allylcarbamates for pro-fluorophore activation and propargyl groups for prodrug activation inside living cells.
Spin or drop coated solutions of ligand-coated nanoparticles act as a novel type of ebeam resist ... more Spin or drop coated solutions of ligand-coated nanoparticles act as a novel type of ebeam resist into which functional nanostructures can be produced in a direct-write process (Y. Ofir et al, Adv. Mater. 20, 2561-2566 (2008)). Thin films were produced by this method from a set of resists in which the size of the Au nanoparticles and ligand length were
ACS nano, Jan 17, 2015
Bacterial biofilms are widely associated with persistent infections. High resistance to conventio... more Bacterial biofilms are widely associated with persistent infections. High resistance to conventional antibiotics and prevalent virulence makes eliminating these bacterial communities challenging therapeutic targets. We describe here the fabrication of a nanoparticle-stabilized capsule with a multicomponent core for the treatment of biofilms. The peppermint oil and cinnamaldehyde combination that comprises the core of the capsules act as potent antimicrobial agents. An in situ reaction at the oil/water interface between the nanoparticles and cinnamaldehyde structurally augments the capsules to efficiently deliver the essential oil payloads, effectively eradicating biofilms of clinically isolated pathogenic bacteria strains. In contrast to their antimicrobial action, the capsules selectively promoted fibroblast proliferation in a mixed bacteria/mammalian cell system making them promising for wound healing applications.
Journal of inorganic biochemistry, Jan 6, 2015
Gold nanorods (GNRs) are important platforms for biosensing and drug delivery. As for most nanoma... more Gold nanorods (GNRs) are important platforms for biosensing and drug delivery. As for most nanomaterials, appropriate coatings such as polyethylene glycol (PEG) are needed to stabilize GNRs within biological fluids. We show here that the interactions of GNRs with proteins can be finely modulated through surface modification using PEG-containing chains bearing charged headgroups. Interestingly, introduction of amino or carboxylate groups produces relevant and differential changes in GNR interactions with three representative proteins: lysozyme, cytochrome c, and bovine serum albumin. These effects were explored through the direct monitoring of plasmonic bands of the GNRs and are supported by independent dynamic light scattering (DLS) and circular dichroism (CD) determinations. Notably, GNR-protein interactions observed for these charged GNRs can be almost completely reversed by salt addition. These observations demonstrate the importance of electrostatic effects in governing GNR-prot...
Bioconjugate chemistry, Jan 20, 2015
Combination therapy employing proteins and small molecules provides access to synergistic treatme... more Combination therapy employing proteins and small molecules provides access to synergistic treatment strategies. Co-delivery of these two payloads is challenging due to the divergent physico-chemical properties of small molecule and protein cargos. Nanoparticle-stabilized nanocapsules (NPSCs) are promising for combination treatment strategies since they have the potential to deliver small molecule drugs and proteins simultaneously into the cytosol. In this study, we loaded paclitaxel into the hydrophobic core of the NPSC and self-assembled caspase-3 and nanoparticles on the capsule surface. The resulting combination NPSCs showed a higher cytotoxicity of than either of the single agent NPSCs, with synergistic action established using combination index values.
Dekker Encyclopedia of Nanoscience and Nanotechnology, Second Edition - Six Volume Set (Print Version), 2008
Colloids and surfaces. B, Biointerfaces, 2015
This work examines how the antimicrobial (killing) activity of net-negative surfaces depends on t... more This work examines how the antimicrobial (killing) activity of net-negative surfaces depends on the presentation of antimicrobial cationic functionality: distributed versus clustered, and flat clusters versus raised clusters. Specifically, the ability to kill Staphylococcus aureus by sparsely distributed 10 nm cationic nanoparticles, immobilized on a negative surface and backfilled with a PEG (polyethylene glycol) brush, was compared with that for a dense layer of the same immobilized nanoparticles. Additionally, sparsely distributed 10 nm poly-L-lysine (PLL) coils, adsorbed to a surface to produce flat cationic "patches" and backfilled with a PEG brush were compared to a saturated adsorbed layer of PLL. The latter resembled classical uniformly cationic antimicrobial surfaces. The protrusion of the cationic clusters substantially influenced killing but the surface concentration of the clusters had minor impact, as long as bacteria adhered. When surfaces were functionalized...
Materials horizons, 2014
The role of nanoparticle surface hydrophobicity on its hemolytic property is established in the a... more The role of nanoparticle surface hydrophobicity on its hemolytic property is established in the absence and the presence of plasma proteins. Significantly, the formation of plasma protein corona on NP surface protects red blood cells from both hydrophilic and hydrophobic NP-mediated hemolysis.
Tetrahedron, 2009
The synthesis of a polypseudorotaxane, polyrotaxane, and polycatenane containing the electron-def... more The synthesis of a polypseudorotaxane, polyrotaxane, and polycatenane containing the electron-deficient cyclophane cyclobis(paraquat-p-phenylene) (CBPQT 4þ ) subunit in the side chain is described. These interlocked supramolecular polymers have been prepared from an azide-functionalized polystyrene derivative and an acetylene-functionalized [2]rotaxane, [2]catenane and their parent tetracationic cyclophane via Cu(I)-catalyzed 1,3 dipolar cycloadditions ('click chemistry'). The synthesis and characterization of the polymers and intermediates has been described using IR, 1 H NMR, UV spectroscopies, and voltammetry. We have shown that the CBPQT 4þ unit of the side chain polystyrene derivative has the ability to reversibly undergo complexation with a complementary dialkoxynaphthalene derivative.
ChemInform, 2001
Trialkyltetrathiafulvalene-σ-Tetracyanoanthraquinodimethane (R 3 TTF-σ-TCNAQ) Diads:
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Papers by Vincent Rotello