Papers by Maurice Lalonde
Journal of Bacteriology, 1983
A method to achieve cell lysis and isolate Frankia sp. plasmid DNA was developed. A screening of ... more A method to achieve cell lysis and isolate Frankia sp. plasmid DNA was developed. A screening of Frankia sp. strains belonging to different host compatibility groups (Alnus sp., Elaeagnus sp., Ceanothus sp.) showed that, of 39 strains tested, 4 (strains Cp11, ARgN22d, ArI3, and EUN1f) possessed plasmids ranging in size from 7.1 to 32.2 kilobase pairs as estimated from agarose gel electrophoresis and electron microscopy. A total of 11 plasmids were detected.
Systematic and Applied Microbiology, 1989
ABSTRACT
Physiologia Plantarum, 1987
ABSTRACT
The first DNA sequences obtained from arbuscular endomycorrhizal fungi are reported. They were ob... more The first DNA sequences obtained from arbuscular endomycorrhizal fungi are reported. They were obtained by directly sequencing overlapping amplified fragments of the nuclear genes coding for the small subunit rRNA. These sequences were used to develop a polymerase chain reaction primer (VANSI) that enables the specific amplification of a portion of the vesicular-arbuscular endomycorrhizal fungus small subunit rRNA directly from a mixture of plant and fungal tissues. The specificity of this primer for arbuscular endomycorrhizal fungi was demonstrated by testing it on a number of organisms and by sequencing the fragment amplified from colonized leek (Allium porum) roots. This approach, coupled with other molecular techniques, will facilitate rapid detection, identification, and possibly quantitation of arbuscular endomycorrhizal fungi.
Genome, 1987
Vegetative and sexual tissues from 22 populations of Alnus crispa (Ait.) Pursh (green alder, Betu... more Vegetative and sexual tissues from 22 populations of Alnus crispa (Ait.) Pursh (green alder, Betulaceae) in Quebec were analyzed for electrophoretically demonstrable diversity of 11 enzymes encoded by 16 structural loci in sexually mature populations. Of these 16 loci, 9 were found polymorphic. A total of 28 different alleles were detected with no more than three alleles per locus. No two-locus linkage disequilibrium was observed between eight polymorphic loci analyzed. Assuming a diploid model, average level of expected heterozygosity was 0.14 in the mature generation, with nearly all populations in Hardy–Weinberg equilibrium for the set of polymorphic loci investigated. Mean outcrossing rate was 0.95. Fixation indices revealed low inbreeding with no specific subpopulation structure. Hence, an important level of gene flow would exist within the populations. Without any strong evidence for polyploidy, and in the light of results obtained, the species could be classified equally well as a diploid or a diploidized allotetraploid. Key words: actinorhizal plant, alder, allozymes, Alnus, fixation indices, heterozygosity, outcrossing rate, polyploidy.
Plant and Soil, 1985
Following the evaluation of the nutritional requirements for thein vitro propagation ofElaeagnus ... more Following the evaluation of the nutritional requirements for thein vitro propagation ofElaeagnus angustifolia, this actinorhizal species was routinely multiplied on MS, supplemented with 100 mM sucrose and 5 μM kinetin. On this medium, at a 3 week-interval, a multiplication rate of 5–10 was observed. A morphological variant occurred in culture (wet type) but it was converted into the normal type
Plant cell reports, 1988
The Escherichia coli β-glucuronidase gene (GUS) was introduced into Alnus incana (L.) Moench prot... more The Escherichia coli β-glucuronidase gene (GUS) was introduced into Alnus incana (L.) Moench protoplasts by electroporation. Level of GUS transient gene expression was increased by increasing DNA concentrations of pBI 221 plasmid and was affected by the amplitude and duration of the applied electric pulse as well as by the presence of polyethylene glycol (PEG) in the electroporation medium. An optimal level of GUS activity was obtained after electroporation with a capacitive discharge of 500 V/cm and 71 ms-duration. This transformation procedure is simple and efficient. These results motivated us to investigate this method as a possible way of achieving the stable transformation of actinorhizal alder.
Plant Cell Tissue and Organ Culture, 1988
Canadian Journal of Microbiology, 1983
Plant Cell Reports, Jun 1, 1988
Canadian Journal of Botany, 1985
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Papers by Maurice Lalonde