Archivos de Zootecnia, v. 60, p. 1263-1270, 2011., 2011
The Amazonian fish known as tambaqui (Colossoma macropomum Cuvier, 1818) is a osseous fish and ha... more The Amazonian fish known as tambaqui (Colossoma macropomum Cuvier, 1818) is a osseous fish and has high commercial value because of its meat quality and for recreational
fishing. Tambaqui is one of the major species in the Amazon basin and found in most rivers and surroundings of Manaus city (Silva et al., 1984). The present study was carried out to evaluate sperm parameters from tambaqui, subjected or not to hormone treatment for a period of 12 months. We used 26 males with three years of age, raised at the Aquaculture Research Center of the National
Department for Drought Control (DNOCS). Animals were identified with magnetic chips and divided into two groups: controls (NI) and hormone-treated (I), which received 2 mg of carp pituitary extract
(CPEkg-1) of body weight (via intra celomic). Semen was collected 14 hours after hormonal induction and the following parameters evaluated: volume, pH, osmolarity and sperm concentration. Fishes had body weight of 5410 g, with total length
and standard length of 68 cm and 59.17 cm, respectively. In the case of hormone-treated and control animals, we found the following criteria, respectively: semen volume of 5.05 mL and 0.55
mL (p<0.05); pH of 8.21 and 8.09; osmolarity of 320.51 and 323 mOsm/kg; sperm concentration of 22.93 x 109 and 40.46 x 109 sptz/mL (p<0,05). Total sperm productions were quantified as 115.79 x 109 sperm/mL in treated fishes and 22.25 x 109
sperm/mL in the controls. We conclude that treatment of adult tambaqui with carp pituitary extract increase semen volume but reduces sperm concentration while had no effect on sperm
osmolarity and pH.
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Papers by Ana Viveiros
three osmolalities (285, 325, and 365 mOsm/kg) plus an undiluted control, and stored at 6-8°C. Motility rate and velocities (curvilinear, straight line, and average path) were determined every other day. Osmolality did not affect the quality of cooled
sperm, thus data were pooled. Motility was higher on d 0 compared to the other days and diluted samples (85-90%) yielded higher motility than control (75%). On d 2, motility was higher in BTS-diluted samples and control, but on d 4 and 6, control
yielded the highest motility. Velocities decreased from d 0 to 6 in diluted samples, but not in control. On d 0, velocities were
higher in BTS-diluted sperm, but, on d 2, 4, and 6, control yielded higher velocities despite of the large variation among males.
Thus P. lineatus sperm should be stored in BTS or without dilution, for a maximum of two days at 6-8°C. Extender osmolality between 285 and 365 mOsm/kg does not affect sperm quality during cold storage.
glucose, and BTS™) - each prepared with 10 different osmolalities - on the initiation and suppression of fresh sperm motility in Prochilodus lineatus and Brycon orbignyanus. Sperm was diluted in each of the 30 solutions and immediately observed under a light microscope to determine which solutions triggered or suppressed the initiation of sperm motility. When present, motility rate
(% motile sperm) was determined at 0, 30, and 120 s post-activation and the motility quality score ranging from 0 (no movement) to 5 (rapidly swimming sperm) was determined at 0 and 30 s post-activation. Osmolality, but not solution composition, significantly affected both motility rate and quality score. Solutions at osmolalities up to 270 mOsm/kg in P. lineatus and up
to 180 mOsm/kg in B. orbignyanus induced motility in at least 60% of sperm, with a minimum quality score of 3.0, and were therefore classified as activating agents. The greatest motility at 0, 30, and 120 s post-activation was observed with solutions ranging from 135 to 225 mOsm/kg for P. lineatus and at 135 mOsm/kg for B. orbignyanus. On the other hand, solutions ranging from 360 to
450 mOsm/kg in P. lineatus and 270 to 450 mOsm/kg in B. orbignyanussuppressed motility in at least 95% of sperm and were classified as immobilizing media. The osmolality of the surrounding medium is the key factor in the initiation or suppression of sperm motility in P. lineatus and B. orbignyanus.
fishing. Tambaqui is one of the major species in the Amazon basin and found in most rivers and surroundings of Manaus city (Silva et al., 1984). The present study was carried out to evaluate sperm parameters from tambaqui, subjected or not to hormone treatment for a period of 12 months. We used 26 males with three years of age, raised at the Aquaculture Research Center of the National
Department for Drought Control (DNOCS). Animals were identified with magnetic chips and divided into two groups: controls (NI) and hormone-treated (I), which received 2 mg of carp pituitary extract
(CPEkg-1) of body weight (via intra celomic). Semen was collected 14 hours after hormonal induction and the following parameters evaluated: volume, pH, osmolarity and sperm concentration. Fishes had body weight of 5410 g, with total length
and standard length of 68 cm and 59.17 cm, respectively. In the case of hormone-treated and control animals, we found the following criteria, respectively: semen volume of 5.05 mL and 0.55
mL (p<0.05); pH of 8.21 and 8.09; osmolarity of 320.51 and 323 mOsm/kg; sperm concentration of 22.93 x 109 and 40.46 x 109 sptz/mL (p<0,05). Total sperm productions were quantified as 115.79 x 109 sperm/mL in treated fishes and 22.25 x 109
sperm/mL in the controls. We conclude that treatment of adult tambaqui with carp pituitary extract increase semen volume but reduces sperm concentration while had no effect on sperm
osmolarity and pH.
methyl glycol (MG) and a control without CPA, were prepared. Immediately after dilution, samples were observed under a light microscope to confirm whether different extender-CPA combinations would suppress the initiation of sperm motility. Motility was then triggered in 92 mOsm/kg NaCl and evaluated immediately after dilution (non-equilibrated samples) and after a 30-min equilibration time at 4°C for motility rate and motility
quality score (0 = no movement; 5 = rapidly swimming sperm). In both species, motility was initiated in all samples diluted in BTS-270-control, Glu-270-MG, Glu-270-control and in all combinations containing DMSO. In B. orbignyanus, motility rate (77 to 92%) and
motility quality score (3.3 to 4.7) of non-equilitrated samples was not significantly affected by any parameter. After 30 min, however, motility quality score decreased in most of the samples, mainly when diluted in BTS™ (3.3 to 4.2). In P. lineatus, motility rate was
significantly higher in non-equilibrated samples (overall mean = 83%) compared to 30-min equilibrated samples (overall mean = 75%). Motility quality score of nonequilibrated samples was not affected by any parameter (3.3 to 4.2), but samples equilibrated in DMSO yielded the lowest score (3.0). Sperm motility (rate and score) was affected differently in B. orbignyanus compared to
P. lineatus, and this finding should be considered when
developing a methodology for sperm cryopreservation.
(Colossoma macropomum Cuvier, 1818) is a
osseous fish and has high commercial value
because of its meat quality and for recreational
fishing. Tambaqui is one of the major species in the
Amazon basin and found in most rivers and
surroundings of Manaus city (Silva et al., 1984).
The present study was carried out to evaluate
sperm parameters from tambaqui, subjected or not
to hormone treatment for a period of 12 months. We
used 26 males with three years of age, raised at
the Aquaculture Research Center of the National
Department for Drought Control (DNOCS). Animals
were identified with magnetic chips and divided
into two groups: controls (NI) and hormone-treated(I), which received 2 mg of carp pituitary extract
(CPEkg-1) of body weight (via intra celomic). Semen
was collected 14 hours after hormonal
induction and the following parameters evaluated:
volume, pH, osmolarity and sperm concentration.
Fishes had body weight of 5410 g, with total length
and standard length of 68 cm and 59.17 cm,
respectively. In the case of hormone-treated and
control animals, we found the following criteria,
respectively: semen volume of 5.05 mL and 0.55
mL (p<0.05); pH of 8.21 and 8.09; osmolarity of
320.51 and 323 mOsm/kg; sperm concentration of
22.93 x 109 and 40.46 x 109 sptz/mL (p<0,05). Total
sperm productions were quantified as 115.79 x
109 sperm/mL in treated fishes and 22.25 x 109
sperm/mL in the controls. We conclude that
treatment of adult tambaqui with carp pituitary
extract increase semen volume but reduces sperm
concentration while had no effect on sperm
osmolarity and pH.
three osmolalities (285, 325, and 365 mOsm/kg) plus an undiluted control, and stored at 6-8°C. Motility rate and velocities (curvilinear, straight line, and average path) were determined every other day. Osmolality did not affect the quality of cooled
sperm, thus data were pooled. Motility was higher on d 0 compared to the other days and diluted samples (85-90%) yielded higher motility than control (75%). On d 2, motility was higher in BTS-diluted samples and control, but on d 4 and 6, control
yielded the highest motility. Velocities decreased from d 0 to 6 in diluted samples, but not in control. On d 0, velocities were
higher in BTS-diluted sperm, but, on d 2, 4, and 6, control yielded higher velocities despite of the large variation among males.
Thus P. lineatus sperm should be stored in BTS or without dilution, for a maximum of two days at 6-8°C. Extender osmolality between 285 and 365 mOsm/kg does not affect sperm quality during cold storage.
glucose, and BTS™) - each prepared with 10 different osmolalities - on the initiation and suppression of fresh sperm motility in Prochilodus lineatus and Brycon orbignyanus. Sperm was diluted in each of the 30 solutions and immediately observed under a light microscope to determine which solutions triggered or suppressed the initiation of sperm motility. When present, motility rate
(% motile sperm) was determined at 0, 30, and 120 s post-activation and the motility quality score ranging from 0 (no movement) to 5 (rapidly swimming sperm) was determined at 0 and 30 s post-activation. Osmolality, but not solution composition, significantly affected both motility rate and quality score. Solutions at osmolalities up to 270 mOsm/kg in P. lineatus and up
to 180 mOsm/kg in B. orbignyanus induced motility in at least 60% of sperm, with a minimum quality score of 3.0, and were therefore classified as activating agents. The greatest motility at 0, 30, and 120 s post-activation was observed with solutions ranging from 135 to 225 mOsm/kg for P. lineatus and at 135 mOsm/kg for B. orbignyanus. On the other hand, solutions ranging from 360 to
450 mOsm/kg in P. lineatus and 270 to 450 mOsm/kg in B. orbignyanussuppressed motility in at least 95% of sperm and were classified as immobilizing media. The osmolality of the surrounding medium is the key factor in the initiation or suppression of sperm motility in P. lineatus and B. orbignyanus.
fishing. Tambaqui is one of the major species in the Amazon basin and found in most rivers and surroundings of Manaus city (Silva et al., 1984). The present study was carried out to evaluate sperm parameters from tambaqui, subjected or not to hormone treatment for a period of 12 months. We used 26 males with three years of age, raised at the Aquaculture Research Center of the National
Department for Drought Control (DNOCS). Animals were identified with magnetic chips and divided into two groups: controls (NI) and hormone-treated (I), which received 2 mg of carp pituitary extract
(CPEkg-1) of body weight (via intra celomic). Semen was collected 14 hours after hormonal induction and the following parameters evaluated: volume, pH, osmolarity and sperm concentration. Fishes had body weight of 5410 g, with total length
and standard length of 68 cm and 59.17 cm, respectively. In the case of hormone-treated and control animals, we found the following criteria, respectively: semen volume of 5.05 mL and 0.55
mL (p<0.05); pH of 8.21 and 8.09; osmolarity of 320.51 and 323 mOsm/kg; sperm concentration of 22.93 x 109 and 40.46 x 109 sptz/mL (p<0,05). Total sperm productions were quantified as 115.79 x 109 sperm/mL in treated fishes and 22.25 x 109
sperm/mL in the controls. We conclude that treatment of adult tambaqui with carp pituitary extract increase semen volume but reduces sperm concentration while had no effect on sperm
osmolarity and pH.
methyl glycol (MG) and a control without CPA, were prepared. Immediately after dilution, samples were observed under a light microscope to confirm whether different extender-CPA combinations would suppress the initiation of sperm motility. Motility was then triggered in 92 mOsm/kg NaCl and evaluated immediately after dilution (non-equilibrated samples) and after a 30-min equilibration time at 4°C for motility rate and motility
quality score (0 = no movement; 5 = rapidly swimming sperm). In both species, motility was initiated in all samples diluted in BTS-270-control, Glu-270-MG, Glu-270-control and in all combinations containing DMSO. In B. orbignyanus, motility rate (77 to 92%) and
motility quality score (3.3 to 4.7) of non-equilitrated samples was not significantly affected by any parameter. After 30 min, however, motility quality score decreased in most of the samples, mainly when diluted in BTS™ (3.3 to 4.2). In P. lineatus, motility rate was
significantly higher in non-equilibrated samples (overall mean = 83%) compared to 30-min equilibrated samples (overall mean = 75%). Motility quality score of nonequilibrated samples was not affected by any parameter (3.3 to 4.2), but samples equilibrated in DMSO yielded the lowest score (3.0). Sperm motility (rate and score) was affected differently in B. orbignyanus compared to
P. lineatus, and this finding should be considered when
developing a methodology for sperm cryopreservation.
(Colossoma macropomum Cuvier, 1818) is a
osseous fish and has high commercial value
because of its meat quality and for recreational
fishing. Tambaqui is one of the major species in the
Amazon basin and found in most rivers and
surroundings of Manaus city (Silva et al., 1984).
The present study was carried out to evaluate
sperm parameters from tambaqui, subjected or not
to hormone treatment for a period of 12 months. We
used 26 males with three years of age, raised at
the Aquaculture Research Center of the National
Department for Drought Control (DNOCS). Animals
were identified with magnetic chips and divided
into two groups: controls (NI) and hormone-treated(I), which received 2 mg of carp pituitary extract
(CPEkg-1) of body weight (via intra celomic). Semen
was collected 14 hours after hormonal
induction and the following parameters evaluated:
volume, pH, osmolarity and sperm concentration.
Fishes had body weight of 5410 g, with total length
and standard length of 68 cm and 59.17 cm,
respectively. In the case of hormone-treated and
control animals, we found the following criteria,
respectively: semen volume of 5.05 mL and 0.55
mL (p<0.05); pH of 8.21 and 8.09; osmolarity of
320.51 and 323 mOsm/kg; sperm concentration of
22.93 x 109 and 40.46 x 109 sptz/mL (p<0,05). Total
sperm productions were quantified as 115.79 x
109 sperm/mL in treated fishes and 22.25 x 109
sperm/mL in the controls. We conclude that
treatment of adult tambaqui with carp pituitary
extract increase semen volume but reduces sperm
concentration while had no effect on sperm
osmolarity and pH.