Rachel Meyer
University of California, Los Angeles, Ecology and Evolutionary Biology, Executive Director of the UC Conservation Genomics Consortium
Address: erasaki Life Sciences Building
610 Charles E Young Drive E
Los Angeles, CA 90095
610 Charles E Young Drive E
Los Angeles, CA 90095
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Papers by Rachel Meyer
employed to map and clone many important disease resistance
(R) genes from Solanum species—especially wild relatives
of potato and tomato. These efforts will increase with
the recent release of potato genome sequence and the impending
release of tomato genome sequence. Most R genes
belong to the prominent nucleotide binding site-leucine rich
repeat (NBS-LRR) class and conserved NBS-LRR protein
motifs enable survey of the R gene space of a plant genome
by generation of resistance gene analogs (RGA), polymerase
chain reaction fragments derived from R genes. We generated
a collection of 97 RGA from the disease-resistant wild
potato S. bulbocastanum, complementing smaller collections
from other Solanum species. To further comparative genomics
approaches, we combined all known Solanum RGA
and cloned solanaceous NBS-LRR gene sequences, nearly
800 sequences in total, into a single meta-analysis. We defined
R gene diversity bins that reflect both evolutionary
relationships and DNA cross-hybridization results. The resulting
framework is amendable and expandable, providing
the research community with a common vocabulary for
present and future study of R gene lineages. Through a
series of sequence and hybridization experiments, we demonstrate
that all tested R gene lineages are of ancient origin,
are shared between Solanum species, and can be successfully
accessed via comparative genomics approaches.
abundant caffeoylquinic acid (CQA) derivatives. Fruit of the
invasive species Solanum viarum Dunal contain numerous complex
CQA derivatives, but only a few have been identified. The
structures of two new compounds isolated from methanolic
extracts of S. viarum fruit by C18-HPLC-DAD were determined
using 2D NMR and MS data. Both include two 5-CQA molecules
joined by glucose via ester and glycosidic linkages. The
structures of compounds 1 and 2 (viarumacids A and B) are,
respectively, 5-caffeoyl- and 3-malonyl-5-caffeoyl-[4-(1β-[6-(5-
caffeoyl)quinate]glucopyranosyl)]quinic acid. The antioxidant
activities determined by ABTS•+ and DPPH• assays were in
the order 1 > 2 > 5-CQA.
employed to map and clone many important disease resistance
(R) genes from Solanum species—especially wild relatives
of potato and tomato. These efforts will increase with
the recent release of potato genome sequence and the impending
release of tomato genome sequence. Most R genes
belong to the prominent nucleotide binding site-leucine rich
repeat (NBS-LRR) class and conserved NBS-LRR protein
motifs enable survey of the R gene space of a plant genome
by generation of resistance gene analogs (RGA), polymerase
chain reaction fragments derived from R genes. We generated
a collection of 97 RGA from the disease-resistant wild
potato S. bulbocastanum, complementing smaller collections
from other Solanum species. To further comparative genomics
approaches, we combined all known Solanum RGA
and cloned solanaceous NBS-LRR gene sequences, nearly
800 sequences in total, into a single meta-analysis. We defined
R gene diversity bins that reflect both evolutionary
relationships and DNA cross-hybridization results. The resulting
framework is amendable and expandable, providing
the research community with a common vocabulary for
present and future study of R gene lineages. Through a
series of sequence and hybridization experiments, we demonstrate
that all tested R gene lineages are of ancient origin,
are shared between Solanum species, and can be successfully
accessed via comparative genomics approaches.
abundant caffeoylquinic acid (CQA) derivatives. Fruit of the
invasive species Solanum viarum Dunal contain numerous complex
CQA derivatives, but only a few have been identified. The
structures of two new compounds isolated from methanolic
extracts of S. viarum fruit by C18-HPLC-DAD were determined
using 2D NMR and MS data. Both include two 5-CQA molecules
joined by glucose via ester and glycosidic linkages. The
structures of compounds 1 and 2 (viarumacids A and B) are,
respectively, 5-caffeoyl- and 3-malonyl-5-caffeoyl-[4-(1β-[6-(5-
caffeoyl)quinate]glucopyranosyl)]quinic acid. The antioxidant
activities determined by ABTS•+ and DPPH• assays were in
the order 1 > 2 > 5-CQA.