Objective: Angiogenesis is necessary for solid tumors to grow and metastasize because it provides... more Objective: Angiogenesis is necessary for solid tumors to grow and metastasize because it provides oxygen and nutrients for the tumor. By considering similar anti-tumor activity by a similar mechanism in herbal medicine, Astragalus hamosus (A. hamosus) was used in this study in a 3D fibrin gel model against human umbilical vein endothelial cells (HUVECs) to investigate its anti-angiogenic properties.Materials and Methods: A. hamosus extract was tested for cytotoxicity on HUVECs using MTT assay. Flow cytometry was used to examine apoptosis, cell cycle, and proliferation. Also, by qPCR, we quantified the expression of genes related to apoptosis, such as caspase-9, -8, -3, and Bcl-2.Results: Angiogenic activities of HUVECs were significantly decreased after treatment with IC50 concentration of A. hamosus extract. Flow cytometry analysis revealed that the cell cycle in HUVECs was arrested in G0/G1 phase in the 3D model higher than 2D culture. Anti-proliferation activity of the extract de...
Culturing adipose-derived stem cells (ADSCs) on the biomimetic ADSC-imprinted substrate is a simp... more Culturing adipose-derived stem cells (ADSCs) on the biomimetic ADSC-imprinted substrate is a simple way for long-term maintenance of their stemness and proliferation potential.
Breast cancer is the most common cancer diagnosed in women, with an estimated 12% of women in the... more Breast cancer is the most common cancer diagnosed in women, with an estimated 12% of women in the United States affected during their lifetime. Researchers have demonstrated that early detection, diagnosis, and treatment are pivotal to increasing survival. The advent of nanotechnology has yielded several novel advances and available modern methods within the clinic to detect and treat breast cancer. Inorganic nanoparticles are broadly utilized for cancer diagnosis and therapeutic purposes. Interestingly, these nanoparticles can also be attached to tumor-specific ligands and used to deliver chemotherapeutic or hormonal agents with high levels of tumor selectivity. Iron oxide nanoparticles are one of the most commonly used nanomaterials, which have attracted much attention to detect and treat breast cancers, owing to their superparamagnetic characteristics. Computerized tomography and magnetic resonance imaging (MRI) utilizing iron-based magnetic nanoparticles are promising approaches...
مقدمه: از كارافتادگی بافت‌های انسانی ناشی از انواع آسیب‌دیدگی‌ها، یكی از پرهزینه‌ترین و جدی‌ترین ... more مقدمه: از كارافتادگی بافت‌های انسانی ناشی از انواع آسیب‌دیدگی‌ها، یكی از پرهزینه‌ترین و جدی‌ترین مشكلات در سلامت انسان است و اثر مستقیم بر كیفیت زندگی دارد. مهندسی بافت، به عنوان یک استراتژی مبتنی بر داربست، از جمله حوزه‌های تحقیقاتی امیدوار كننده‌ای است كه می‌تواند علاوه بر فراهم كردن بافت و ارگان برای پیوند، چشم‌انداز جدیدی را برای درمان بیماران باز كند. دانشمندان حوزه‌های مختلف كوشیده‌اند تا با وظیفه‌مند كردن داربست، به تعاملات سطحی سلول‌های خاص دست یابند. روش‌ها: پودر سلولز با استفاده از گاز 2NO اکسید شد و داربست متخلخل به روش پرس خشک آماده گردید. پپتید RGD به سطح داربست متصل گردید تا یک داربست هیبریدی ساخته شود. داربست با FTIR (Fourier transform infrared spectroscopy) و میکروسکوپ الکترونی روبشی (SEM یا Scanning electron microscope) مشخصه‌یابی شد و زیست‌سازگاری آن با استفاده از آزمون MTT [3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide] ارزیابی گردید. نتایج FTIR، اکسیداسیون سلولز و تشکیل پیوند بین سطح داربست و RGD را تأیید کرد. ریزساختار متخلخل با اندازه‌ی تخلخل منا...
The study was aimed at design a good fusion construct that would successfully express the recombi... more The study was aimed at design a good fusion construct that would successfully express the recombinant proteins and produce peptides in Escherichia coli. Two different constructs including human epidermal growth factor (hEGF) gene were designed to obtain an effi cient expression level of hEGF. The hEGF sequence was inserted in pET32a vector containing thioredoxin (Trx) sequence and modifi ed pET15b vector containing intein and elastin-like polypeptide (ELP). METHODS: The vectors were transformed into E. coli TOP10F′ for multiplication and further into E. coli BL21 (DE3) to express protein. The hEGF expression was induced by isopropyl β-D-1-thiogalactopyranoside (IPTG) while the expression levels were evaluated by SDS-PAGE and western blotting and compared by ImageJ analysis, BCA and Elisa assays. RESULTS: The expression level after 2 hours of IPTG induction was signifi cantly higher than after other induction times. ImageJ, BCA and Elisa analyses demonstrated that the Trx presence enhanced protein expression signifi cantly when compared to ELP-intein-based construct. CONCLUSION: The pET32a-Trx-hEGF construct had a higher expression than pET15b-ELP-intein-hEGF. Overall, considering Trx, the fusion protein in construct design can make it suitable to signifi cantly express hEGF compared to ELP-intein while its combination with ELP-intein may improve the expression of the ELPintein construct (Tab. 2, Fig. 7, Ref. 34).
Silk fibroin (SF) is a natural fibrous polymer with strong potential for many biomedical applicat... more Silk fibroin (SF) is a natural fibrous polymer with strong potential for many biomedical applications. SF has attracted interest in the field of bone tissue engineering due to its extraordinary characteristics in terms of elasticity, flexibility, biocompatibility and biodegradability. However, low osteogenic capacity has limited applications for SF in the orthopedic arena unless suitably functionalized. Hydroxyapatite (HAp) is a well-established bioceramic with biocompatibility and appropriate for constructing orthopedic and dental substitutes. However, HAp ceramics tend to be brittle which can restrict applications in the repair of load-bearing tissues such as bones. Therefore, blending SF and HAp combines the useful properties of both materials as bone constructs for tissue engineering, the subject of this review.
This study evaluated tracheal regeneration studies using scientometric and co-occurrence analysis... more This study evaluated tracheal regeneration studies using scientometric and co-occurrence analysis to identify the most important topics and assess their trends over time. To provide the adequate search options, PubMed, Scopus, and Web of Science (WOS) were used to cover various categories such as keywords, countries, organizations, and authors. Search results were obtained by employing Bibexcel. Co-occurrence analysis was applied to evaluate the publications. Finally, scientific maps, author's network, and country contributions were depicted using VOSviewer and NetDraw. Furthermore, the first 25 countries and 130 of the most productive authors were determined. Regarding the trend analysis, 10 co-occurrence terms out of highly frequent words were examined at 5-year intervals. Our findings indicated that the field of trachea regeneration has tested different approaches over the time. In total, 65 countries have contributed to scientific progress both in experimental and clinical fields. Special keywords such as tissue engineering and different types of stem cells have been increasingly used since 1995. Studies have addressed topics such as angiogenesis, decellularization methods, extracellular matrix, and mechanical properties since 2011. These findings will offer evidence-based information about the current status and trends of tracheal replacement research topics over time, as well as countries' contributions.
Artificial Cells, Nanomedicine, and Biotechnology, 2013
Cellulose powder was oxidized by NO2 gas and the porous scaffold was fabricated by dry pressing. ... more Cellulose powder was oxidized by NO2 gas and the porous scaffold was fabricated by dry pressing. RGD peptide was immobilized on the surface of scaffold by grafting to make a hybrid scaffold. The hybrid scaffold was characterized by SEM and FTIR and its biocompatibility was examined through MTT assay. FTIR results proved oxidization of cellulose and bonding between scaffold surface and RGD. Porous microstructure having suitable size was confirmed by SEM. The results by MTT showed significant increase of viable cells on hybrid scaffold. Porous structure and high biocompatibility were the benefits of scaffold in bone tissue engineering.
Journal of Biomedical Materials Research Part B: Applied Biomaterials, 2013
In this research, we studied improvement of mechanical properties of dimethacrylate-silica based ... more In this research, we studied improvement of mechanical properties of dimethacrylate-silica based dental composites by addition of Fuller's Earth (FE) clay. Three composites were made as base compounds consisting of 68, 58, and 48 wt % resin and 31, 41, and 51 wt % silica, respectively. Afterward, the composites were modified by adding FE. Mechanical properties including flexural strength, flexural modulus, work-of-fracture, fracture toughness, and microhardness were measured. Clay particles and fracture surface of composites consisting of 51 wt % silica (with and without FE) were examined by SEM. Measured results showed that flexural strength, work-of-fracture, flexural modulus, and microhardness of all composites increased by including FE nanofibers. Fracture toughness except for composite including 51 wt % silica had similar variations. It seems that locating FE nanofibers in weak resin region among silica particles leads to strengthening mechanisms, such as bridging and crack deflection, which cause improvement in mechanical properties. V
Journal of Biomedical Materials Research Part A, 2012
Hydroxyapatite (HAp) coatings were applied using sol-gel method. Phosphor pentoxide and calcium n... more Hydroxyapatite (HAp) coatings were applied using sol-gel method. Phosphor pentoxide and calcium nitrate were used as phosphorous and calcium precursors, respectively. Zinc nitrate and silver nitrate were used as substitute of calcium in HAp structure. As a base concentration, 1.5 wt %Ag and 2.5 wt %Zn were used. The weight percent of Ag was increased at 0.3 wt% and Zn content was scaled down at 0.5 wt%. Phase analysis and chemical bonds of synthesized materials were studied by XRD and FTIR. Antibacterial activity of Ag- and Zn-doped samples against methicilin-resistant Staphylococcus aureus (MRSA) were assessed by the plate-counting method. The XRD and FTIR results proved formation of HAp compound. Colony counting showed that silver and zinc ions prevent proliferation and growth of MRSA. Interestingly, co-presence of metal ions improves the antibacterial effectiveness of the coatings and the combined effect was greater than sum of the individual effects when each was administered alone. Overall, synergism between antibacterial activities of Zn(2+) and Ag(+) ions against MRSA can be suggested. Thus, cell toxicity decreases and biocompatibility increases without any decrement in antibacterial activity.
International Journal of Polymeric Materials and Polymeric Biomaterials, 2020
Abstract In this study, we introduced a novel and modified method to synthesize photocrosslinkabl... more Abstract In this study, we introduced a novel and modified method to synthesize photocrosslinkable chitosan hydrogel. Three methacrylated samples under two concentrations of methacrylic anhydride and using EDC/NHS as a crosslinker were prepared. Methacrylated chitosan obtained under controlled conditions and using EDC/NHS had significant coupling efficiency and capacity of gel formation compared to other samples. Diluted culture media provided a favorable solubility condition for methacrylated chitosan and could be tolerated by cells during gelation time. The hydrogels included interconnected pores ranging 100–300 µm and swelling ratios of 40–400%. The results opened the new aspects for developing novel biomaterials in cell-based therapies. Graphical Abstract
This study evaluated tracheal regeneration studies using scientometric and co-occurrence analysis... more This study evaluated tracheal regeneration studies using scientometric and co-occurrence analysis to identify the most important topics and assess their trends over time. To provide the adequate search options, PubMed, Scopus, andWeb of Science (WOS) were used to cover various categories such as keywords, countries, organizations, and authors. Search results were obtained by employing Bibexcel. Co-occurrence analysis was applied to evaluate the publications. Finally, scientific maps, author’s network, and country contributions were depicted using VOSviewer and NetDraw. Furthermore, the first 25 countries and 130 of the most productive authors were determined. Regarding the trend analysis, 10 co-occurrence terms out of highly frequent words were examined at 5-year intervals. Our findings indicated that the field of trachea regeneration has tested different approaches over the time. In total, 65 countries have contributed to scientific progress both in experimental and clinical fields. Special keywords such as tissue engineering and different types of stem cells have been increasingly used since 1995. Studies have addressed topics such as angiogenesis, decellularization methods, extracellular matrix, and mechanical properties since 2011. These findings will offer evidence-based information about the current status and trends of tracheal replacement research topics over time, as well as countries’ contributions.
In this research, we studied improvement of mechanical properties of dimethacrylate-silica based ... more In this research, we studied improvement of mechanical properties of dimethacrylate-silica based dental composites by addition of Fuller's Earth (FE) clay. Three composites were made as base compounds consisting of 68, 58, and 48 wt % resin and 31, 41, and 51 wt % silica, respectively. Afterward, the composites were modified by adding FE. Mechanical properties including flexural strength, flexural modulus, work-of-fracture, fracture toughness, and microhardness were measured. Clay particles and fracture surface of composites consisting of 51 wt % silica (with and without FE) were examined by SEM. Measured results showed that flexural strength, work-of-fracture, flexural modulus, and microhardness of all composites increased by including FE nanofibers. Fracture toughness except for composite including 51 wt % silica had similar variations. It seems that locating FE nanofibers in weak resin region among silica particles leads to strengthening mechanisms, such as bridging and crack deflection, which cause improvement in mechanical properties.
Journal of Biomedical Materials Research Part B: Applied Biomaterials, Volume 101B, Issue 6, pages 911–918, August 2013
Cellulose powder was oxidized by NO2 gas and the porous
scaffold was fabricated by dry pressing. ... more Cellulose powder was oxidized by NO2 gas and the porous scaffold was fabricated by dry pressing. RGD peptide was immobilized on the surface of scaffold by grafting to make a hybrid scaffold. The hybrid scaffold was characterized by SEM and FTIR and its biocompatibility was examined through MTT assay. FTIR results proved oxidization of cellulose and bonding between scaffold surface and RGD. Porous microstructure having suitable size was confirmed by SEM. The results by MTT showed significant increase of viable cells on hybrid scaffold. Porous structure and high biocompatibility were the benefits of scaffold in bone tissue engineering. Artificial Cells, Nanomedicine, and Biotechnology: An International Journal, Volume 41, Issue 6, 2013
Journal of Biomedical Materials Research Part A, 2013
Hydroxyapatite (HAp) coatings were applied using
sol–gel method. Phosphor pentoxide and calcium n... more Hydroxyapatite (HAp) coatings were applied using sol–gel method. Phosphor pentoxide and calcium nitrate were used as phosphorous and calcium precursors, respectively. Zinc nitrate and silver nitrate were used as substitute of calcium in HAp structure. As a base concentration, 1.5 wt %Ag and 2.5 wt %Zn were used. The weight percent of Ag was increased at 0.3 wt% and Zn content was scaled down at 0.5 wt%. Phase analysis and chemical bonds of synthesized materials were studied by XRD and FTIR. Antibacterial activity of Ag- and Zn-doped samples against methicilin-resistant Staphylococcus aureus (MRSA) were assessed by the platecounting method. The XRD and FTIR results proved formation of HAp compound. Colony counting showed that silver and zinc ions prevent proliferation and growth of MRSA. Interestingly, co-presence of metal ions improves the antibacterial effectiveness of the coatings and the combined effect was greater than sum of the individual effects when each was administered alone. Overall, synergism between antibacterial activities of Zn2þ and Agþ ions against MRSA can be suggested. Thus, cell toxicity decreases and biocompatibility increases without any decrement in antibacterial activity. Journal of Biomedical Materials Research Part A 101 (1), 222-230, 2013
Objective: Angiogenesis is necessary for solid tumors to grow and metastasize because it provides... more Objective: Angiogenesis is necessary for solid tumors to grow and metastasize because it provides oxygen and nutrients for the tumor. By considering similar anti-tumor activity by a similar mechanism in herbal medicine, Astragalus hamosus (A. hamosus) was used in this study in a 3D fibrin gel model against human umbilical vein endothelial cells (HUVECs) to investigate its anti-angiogenic properties.Materials and Methods: A. hamosus extract was tested for cytotoxicity on HUVECs using MTT assay. Flow cytometry was used to examine apoptosis, cell cycle, and proliferation. Also, by qPCR, we quantified the expression of genes related to apoptosis, such as caspase-9, -8, -3, and Bcl-2.Results: Angiogenic activities of HUVECs were significantly decreased after treatment with IC50 concentration of A. hamosus extract. Flow cytometry analysis revealed that the cell cycle in HUVECs was arrested in G0/G1 phase in the 3D model higher than 2D culture. Anti-proliferation activity of the extract de...
Culturing adipose-derived stem cells (ADSCs) on the biomimetic ADSC-imprinted substrate is a simp... more Culturing adipose-derived stem cells (ADSCs) on the biomimetic ADSC-imprinted substrate is a simple way for long-term maintenance of their stemness and proliferation potential.
Breast cancer is the most common cancer diagnosed in women, with an estimated 12% of women in the... more Breast cancer is the most common cancer diagnosed in women, with an estimated 12% of women in the United States affected during their lifetime. Researchers have demonstrated that early detection, diagnosis, and treatment are pivotal to increasing survival. The advent of nanotechnology has yielded several novel advances and available modern methods within the clinic to detect and treat breast cancer. Inorganic nanoparticles are broadly utilized for cancer diagnosis and therapeutic purposes. Interestingly, these nanoparticles can also be attached to tumor-specific ligands and used to deliver chemotherapeutic or hormonal agents with high levels of tumor selectivity. Iron oxide nanoparticles are one of the most commonly used nanomaterials, which have attracted much attention to detect and treat breast cancers, owing to their superparamagnetic characteristics. Computerized tomography and magnetic resonance imaging (MRI) utilizing iron-based magnetic nanoparticles are promising approaches...
مقدمه: از كارافتادگی بافت‌های انسانی ناشی از انواع آسیب‌دیدگی‌ها، یكی از پرهزینه‌ترین و جدی‌ترین ... more مقدمه: از كارافتادگی بافت‌های انسانی ناشی از انواع آسیب‌دیدگی‌ها، یكی از پرهزینه‌ترین و جدی‌ترین مشكلات در سلامت انسان است و اثر مستقیم بر كیفیت زندگی دارد. مهندسی بافت، به عنوان یک استراتژی مبتنی بر داربست، از جمله حوزه‌های تحقیقاتی امیدوار كننده‌ای است كه می‌تواند علاوه بر فراهم كردن بافت و ارگان برای پیوند، چشم‌انداز جدیدی را برای درمان بیماران باز كند. دانشمندان حوزه‌های مختلف كوشیده‌اند تا با وظیفه‌مند كردن داربست، به تعاملات سطحی سلول‌های خاص دست یابند. روش‌ها: پودر سلولز با استفاده از گاز 2NO اکسید شد و داربست متخلخل به روش پرس خشک آماده گردید. پپتید RGD به سطح داربست متصل گردید تا یک داربست هیبریدی ساخته شود. داربست با FTIR (Fourier transform infrared spectroscopy) و میکروسکوپ الکترونی روبشی (SEM یا Scanning electron microscope) مشخصه‌یابی شد و زیست‌سازگاری آن با استفاده از آزمون MTT [3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide] ارزیابی گردید. نتایج FTIR، اکسیداسیون سلولز و تشکیل پیوند بین سطح داربست و RGD را تأیید کرد. ریزساختار متخلخل با اندازه‌ی تخلخل منا...
The study was aimed at design a good fusion construct that would successfully express the recombi... more The study was aimed at design a good fusion construct that would successfully express the recombinant proteins and produce peptides in Escherichia coli. Two different constructs including human epidermal growth factor (hEGF) gene were designed to obtain an effi cient expression level of hEGF. The hEGF sequence was inserted in pET32a vector containing thioredoxin (Trx) sequence and modifi ed pET15b vector containing intein and elastin-like polypeptide (ELP). METHODS: The vectors were transformed into E. coli TOP10F′ for multiplication and further into E. coli BL21 (DE3) to express protein. The hEGF expression was induced by isopropyl β-D-1-thiogalactopyranoside (IPTG) while the expression levels were evaluated by SDS-PAGE and western blotting and compared by ImageJ analysis, BCA and Elisa assays. RESULTS: The expression level after 2 hours of IPTG induction was signifi cantly higher than after other induction times. ImageJ, BCA and Elisa analyses demonstrated that the Trx presence enhanced protein expression signifi cantly when compared to ELP-intein-based construct. CONCLUSION: The pET32a-Trx-hEGF construct had a higher expression than pET15b-ELP-intein-hEGF. Overall, considering Trx, the fusion protein in construct design can make it suitable to signifi cantly express hEGF compared to ELP-intein while its combination with ELP-intein may improve the expression of the ELPintein construct (Tab. 2, Fig. 7, Ref. 34).
Silk fibroin (SF) is a natural fibrous polymer with strong potential for many biomedical applicat... more Silk fibroin (SF) is a natural fibrous polymer with strong potential for many biomedical applications. SF has attracted interest in the field of bone tissue engineering due to its extraordinary characteristics in terms of elasticity, flexibility, biocompatibility and biodegradability. However, low osteogenic capacity has limited applications for SF in the orthopedic arena unless suitably functionalized. Hydroxyapatite (HAp) is a well-established bioceramic with biocompatibility and appropriate for constructing orthopedic and dental substitutes. However, HAp ceramics tend to be brittle which can restrict applications in the repair of load-bearing tissues such as bones. Therefore, blending SF and HAp combines the useful properties of both materials as bone constructs for tissue engineering, the subject of this review.
This study evaluated tracheal regeneration studies using scientometric and co-occurrence analysis... more This study evaluated tracheal regeneration studies using scientometric and co-occurrence analysis to identify the most important topics and assess their trends over time. To provide the adequate search options, PubMed, Scopus, and Web of Science (WOS) were used to cover various categories such as keywords, countries, organizations, and authors. Search results were obtained by employing Bibexcel. Co-occurrence analysis was applied to evaluate the publications. Finally, scientific maps, author's network, and country contributions were depicted using VOSviewer and NetDraw. Furthermore, the first 25 countries and 130 of the most productive authors were determined. Regarding the trend analysis, 10 co-occurrence terms out of highly frequent words were examined at 5-year intervals. Our findings indicated that the field of trachea regeneration has tested different approaches over the time. In total, 65 countries have contributed to scientific progress both in experimental and clinical fields. Special keywords such as tissue engineering and different types of stem cells have been increasingly used since 1995. Studies have addressed topics such as angiogenesis, decellularization methods, extracellular matrix, and mechanical properties since 2011. These findings will offer evidence-based information about the current status and trends of tracheal replacement research topics over time, as well as countries' contributions.
Artificial Cells, Nanomedicine, and Biotechnology, 2013
Cellulose powder was oxidized by NO2 gas and the porous scaffold was fabricated by dry pressing. ... more Cellulose powder was oxidized by NO2 gas and the porous scaffold was fabricated by dry pressing. RGD peptide was immobilized on the surface of scaffold by grafting to make a hybrid scaffold. The hybrid scaffold was characterized by SEM and FTIR and its biocompatibility was examined through MTT assay. FTIR results proved oxidization of cellulose and bonding between scaffold surface and RGD. Porous microstructure having suitable size was confirmed by SEM. The results by MTT showed significant increase of viable cells on hybrid scaffold. Porous structure and high biocompatibility were the benefits of scaffold in bone tissue engineering.
Journal of Biomedical Materials Research Part B: Applied Biomaterials, 2013
In this research, we studied improvement of mechanical properties of dimethacrylate-silica based ... more In this research, we studied improvement of mechanical properties of dimethacrylate-silica based dental composites by addition of Fuller's Earth (FE) clay. Three composites were made as base compounds consisting of 68, 58, and 48 wt % resin and 31, 41, and 51 wt % silica, respectively. Afterward, the composites were modified by adding FE. Mechanical properties including flexural strength, flexural modulus, work-of-fracture, fracture toughness, and microhardness were measured. Clay particles and fracture surface of composites consisting of 51 wt % silica (with and without FE) were examined by SEM. Measured results showed that flexural strength, work-of-fracture, flexural modulus, and microhardness of all composites increased by including FE nanofibers. Fracture toughness except for composite including 51 wt % silica had similar variations. It seems that locating FE nanofibers in weak resin region among silica particles leads to strengthening mechanisms, such as bridging and crack deflection, which cause improvement in mechanical properties. V
Journal of Biomedical Materials Research Part A, 2012
Hydroxyapatite (HAp) coatings were applied using sol-gel method. Phosphor pentoxide and calcium n... more Hydroxyapatite (HAp) coatings were applied using sol-gel method. Phosphor pentoxide and calcium nitrate were used as phosphorous and calcium precursors, respectively. Zinc nitrate and silver nitrate were used as substitute of calcium in HAp structure. As a base concentration, 1.5 wt %Ag and 2.5 wt %Zn were used. The weight percent of Ag was increased at 0.3 wt% and Zn content was scaled down at 0.5 wt%. Phase analysis and chemical bonds of synthesized materials were studied by XRD and FTIR. Antibacterial activity of Ag- and Zn-doped samples against methicilin-resistant Staphylococcus aureus (MRSA) were assessed by the plate-counting method. The XRD and FTIR results proved formation of HAp compound. Colony counting showed that silver and zinc ions prevent proliferation and growth of MRSA. Interestingly, co-presence of metal ions improves the antibacterial effectiveness of the coatings and the combined effect was greater than sum of the individual effects when each was administered alone. Overall, synergism between antibacterial activities of Zn(2+) and Ag(+) ions against MRSA can be suggested. Thus, cell toxicity decreases and biocompatibility increases without any decrement in antibacterial activity.
International Journal of Polymeric Materials and Polymeric Biomaterials, 2020
Abstract In this study, we introduced a novel and modified method to synthesize photocrosslinkabl... more Abstract In this study, we introduced a novel and modified method to synthesize photocrosslinkable chitosan hydrogel. Three methacrylated samples under two concentrations of methacrylic anhydride and using EDC/NHS as a crosslinker were prepared. Methacrylated chitosan obtained under controlled conditions and using EDC/NHS had significant coupling efficiency and capacity of gel formation compared to other samples. Diluted culture media provided a favorable solubility condition for methacrylated chitosan and could be tolerated by cells during gelation time. The hydrogels included interconnected pores ranging 100–300 µm and swelling ratios of 40–400%. The results opened the new aspects for developing novel biomaterials in cell-based therapies. Graphical Abstract
This study evaluated tracheal regeneration studies using scientometric and co-occurrence analysis... more This study evaluated tracheal regeneration studies using scientometric and co-occurrence analysis to identify the most important topics and assess their trends over time. To provide the adequate search options, PubMed, Scopus, andWeb of Science (WOS) were used to cover various categories such as keywords, countries, organizations, and authors. Search results were obtained by employing Bibexcel. Co-occurrence analysis was applied to evaluate the publications. Finally, scientific maps, author’s network, and country contributions were depicted using VOSviewer and NetDraw. Furthermore, the first 25 countries and 130 of the most productive authors were determined. Regarding the trend analysis, 10 co-occurrence terms out of highly frequent words were examined at 5-year intervals. Our findings indicated that the field of trachea regeneration has tested different approaches over the time. In total, 65 countries have contributed to scientific progress both in experimental and clinical fields. Special keywords such as tissue engineering and different types of stem cells have been increasingly used since 1995. Studies have addressed topics such as angiogenesis, decellularization methods, extracellular matrix, and mechanical properties since 2011. These findings will offer evidence-based information about the current status and trends of tracheal replacement research topics over time, as well as countries’ contributions.
In this research, we studied improvement of mechanical properties of dimethacrylate-silica based ... more In this research, we studied improvement of mechanical properties of dimethacrylate-silica based dental composites by addition of Fuller's Earth (FE) clay. Three composites were made as base compounds consisting of 68, 58, and 48 wt % resin and 31, 41, and 51 wt % silica, respectively. Afterward, the composites were modified by adding FE. Mechanical properties including flexural strength, flexural modulus, work-of-fracture, fracture toughness, and microhardness were measured. Clay particles and fracture surface of composites consisting of 51 wt % silica (with and without FE) were examined by SEM. Measured results showed that flexural strength, work-of-fracture, flexural modulus, and microhardness of all composites increased by including FE nanofibers. Fracture toughness except for composite including 51 wt % silica had similar variations. It seems that locating FE nanofibers in weak resin region among silica particles leads to strengthening mechanisms, such as bridging and crack deflection, which cause improvement in mechanical properties.
Journal of Biomedical Materials Research Part B: Applied Biomaterials, Volume 101B, Issue 6, pages 911–918, August 2013
Cellulose powder was oxidized by NO2 gas and the porous
scaffold was fabricated by dry pressing. ... more Cellulose powder was oxidized by NO2 gas and the porous scaffold was fabricated by dry pressing. RGD peptide was immobilized on the surface of scaffold by grafting to make a hybrid scaffold. The hybrid scaffold was characterized by SEM and FTIR and its biocompatibility was examined through MTT assay. FTIR results proved oxidization of cellulose and bonding between scaffold surface and RGD. Porous microstructure having suitable size was confirmed by SEM. The results by MTT showed significant increase of viable cells on hybrid scaffold. Porous structure and high biocompatibility were the benefits of scaffold in bone tissue engineering. Artificial Cells, Nanomedicine, and Biotechnology: An International Journal, Volume 41, Issue 6, 2013
Journal of Biomedical Materials Research Part A, 2013
Hydroxyapatite (HAp) coatings were applied using
sol–gel method. Phosphor pentoxide and calcium n... more Hydroxyapatite (HAp) coatings were applied using sol–gel method. Phosphor pentoxide and calcium nitrate were used as phosphorous and calcium precursors, respectively. Zinc nitrate and silver nitrate were used as substitute of calcium in HAp structure. As a base concentration, 1.5 wt %Ag and 2.5 wt %Zn were used. The weight percent of Ag was increased at 0.3 wt% and Zn content was scaled down at 0.5 wt%. Phase analysis and chemical bonds of synthesized materials were studied by XRD and FTIR. Antibacterial activity of Ag- and Zn-doped samples against methicilin-resistant Staphylococcus aureus (MRSA) were assessed by the platecounting method. The XRD and FTIR results proved formation of HAp compound. Colony counting showed that silver and zinc ions prevent proliferation and growth of MRSA. Interestingly, co-presence of metal ions improves the antibacterial effectiveness of the coatings and the combined effect was greater than sum of the individual effects when each was administered alone. Overall, synergism between antibacterial activities of Zn2þ and Agþ ions against MRSA can be suggested. Thus, cell toxicity decreases and biocompatibility increases without any decrement in antibacterial activity. Journal of Biomedical Materials Research Part A 101 (1), 222-230, 2013
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Papers by Saeed Samani
most important topics and assess their trends over time. To provide the adequate search options, PubMed, Scopus, andWeb of Science (WOS) were used to cover various categories such as keywords, countries, organizations, and authors. Search results were obtained by employing Bibexcel. Co-occurrence analysis was applied to evaluate the publications. Finally, scientific maps, author’s network, and country contributions were depicted using VOSviewer
and NetDraw. Furthermore, the first 25 countries and 130 of the most productive authors were determined.
Regarding the trend analysis, 10 co-occurrence terms out of highly frequent words were examined at 5-year
intervals. Our findings indicated that the field of trachea regeneration has tested different approaches over the time. In total, 65 countries have contributed to scientific progress both in experimental and clinical fields. Special
keywords such as tissue engineering and different types of stem cells have been increasingly used since 1995.
Studies have addressed topics such as angiogenesis, decellularization methods, extracellular matrix, and mechanical properties since 2011. These findings will offer evidence-based information about the current status and trends of tracheal replacement research topics over time, as well as countries’ contributions.
Journal of Biomedical Materials Research Part B: Applied Biomaterials, Volume 101B, Issue 6, pages 911–918, August 2013
scaffold was fabricated by dry pressing. RGD peptide was immobilized on the surface of scaffold by grafting to make a hybrid scaffold. The hybrid scaffold was characterized by SEM and FTIR and its biocompatibility was examined through MTT assay. FTIR results proved oxidization of cellulose and bonding between scaffold surface and RGD. Porous microstructure having suitable size was confirmed by SEM. The results by MTT showed significant increase of viable cells on hybrid scaffold. Porous structure and high biocompatibility were the benefits of scaffold in bone tissue engineering. Artificial Cells, Nanomedicine, and Biotechnology: An International Journal, Volume 41, Issue 6, 2013
sol–gel method. Phosphor pentoxide and calcium nitrate
were used as phosphorous and calcium precursors, respectively.
Zinc nitrate and silver nitrate were used as substitute
of calcium in HAp structure. As a base concentration, 1.5 wt
%Ag and 2.5 wt %Zn were used. The weight percent of Ag
was increased at 0.3 wt% and Zn content was scaled down at
0.5 wt%. Phase analysis and chemical bonds of synthesized
materials were studied by XRD and FTIR. Antibacterial activity
of Ag- and Zn-doped samples against methicilin-resistant
Staphylococcus aureus (MRSA) were assessed by the platecounting
method. The XRD and FTIR results proved formation
of HAp compound. Colony counting showed that silver
and zinc ions prevent proliferation and growth of MRSA.
Interestingly, co-presence of metal ions improves the antibacterial effectiveness of the coatings and the combined effect was greater than sum of the individual effects when each was administered alone. Overall, synergism between antibacterial activities of Zn2þ and Agþ ions against MRSA can be suggested. Thus, cell toxicity decreases and biocompatibility increases without any decrement in antibacterial activity. Journal of Biomedical Materials Research Part A 101 (1), 222-230, 2013
most important topics and assess their trends over time. To provide the adequate search options, PubMed, Scopus, andWeb of Science (WOS) were used to cover various categories such as keywords, countries, organizations, and authors. Search results were obtained by employing Bibexcel. Co-occurrence analysis was applied to evaluate the publications. Finally, scientific maps, author’s network, and country contributions were depicted using VOSviewer
and NetDraw. Furthermore, the first 25 countries and 130 of the most productive authors were determined.
Regarding the trend analysis, 10 co-occurrence terms out of highly frequent words were examined at 5-year
intervals. Our findings indicated that the field of trachea regeneration has tested different approaches over the time. In total, 65 countries have contributed to scientific progress both in experimental and clinical fields. Special
keywords such as tissue engineering and different types of stem cells have been increasingly used since 1995.
Studies have addressed topics such as angiogenesis, decellularization methods, extracellular matrix, and mechanical properties since 2011. These findings will offer evidence-based information about the current status and trends of tracheal replacement research topics over time, as well as countries’ contributions.
Journal of Biomedical Materials Research Part B: Applied Biomaterials, Volume 101B, Issue 6, pages 911–918, August 2013
scaffold was fabricated by dry pressing. RGD peptide was immobilized on the surface of scaffold by grafting to make a hybrid scaffold. The hybrid scaffold was characterized by SEM and FTIR and its biocompatibility was examined through MTT assay. FTIR results proved oxidization of cellulose and bonding between scaffold surface and RGD. Porous microstructure having suitable size was confirmed by SEM. The results by MTT showed significant increase of viable cells on hybrid scaffold. Porous structure and high biocompatibility were the benefits of scaffold in bone tissue engineering. Artificial Cells, Nanomedicine, and Biotechnology: An International Journal, Volume 41, Issue 6, 2013
sol–gel method. Phosphor pentoxide and calcium nitrate
were used as phosphorous and calcium precursors, respectively.
Zinc nitrate and silver nitrate were used as substitute
of calcium in HAp structure. As a base concentration, 1.5 wt
%Ag and 2.5 wt %Zn were used. The weight percent of Ag
was increased at 0.3 wt% and Zn content was scaled down at
0.5 wt%. Phase analysis and chemical bonds of synthesized
materials were studied by XRD and FTIR. Antibacterial activity
of Ag- and Zn-doped samples against methicilin-resistant
Staphylococcus aureus (MRSA) were assessed by the platecounting
method. The XRD and FTIR results proved formation
of HAp compound. Colony counting showed that silver
and zinc ions prevent proliferation and growth of MRSA.
Interestingly, co-presence of metal ions improves the antibacterial effectiveness of the coatings and the combined effect was greater than sum of the individual effects when each was administered alone. Overall, synergism between antibacterial activities of Zn2þ and Agþ ions against MRSA can be suggested. Thus, cell toxicity decreases and biocompatibility increases without any decrement in antibacterial activity. Journal of Biomedical Materials Research Part A 101 (1), 222-230, 2013