Yildiz Uludag

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I have degree in chemical engineering (BSc; METU, Turkey), biotechnology (PhD; Cranfield University, UK) and 10 years professional experience in the area of biosensors. After completing a master’s degree in biochemistry (MSc; GYTE, Turkey), I worked at two Cambridge University spin-off companies (Affinity Sensors and Akubio Ltd.) at different positions. Some of the experiences I obtained during my professional work and PhD study include sensor chip fabrication, sensor surface chemistry development, biological assay development (for the detection of proteins, DNA, virus and bacteria), sensor signal enhancement via nanoparticles and product development from idea to market. Not only did I use different commercial biosensor instruments (optical, piezoelectric and electrochemical) but was also involved in the development of new biosensor devices and sensor chips from prototype to commercial products, and some of the products I developed or was involved in its development are in the market.

Biosensor technology is an exciting area that involves many disciplines. From physics to understand how the instruments work, to the knowledge of immunoassays, protein-protein interaction, DNA hybridisation, viral and bacterial detection, it involves many interesting areas. One of the most valuable contributions of biosensors is that they allow detection of clinically relevant molecules, so they are very important diagnostic tools of our day.

Research interests

Molecular recognition, sensing and diagnostics

Nanotechnology application in analytical and life sciences

Genetic and protein biomarker selection and detection

Biosensor assay development and signal enhancement

Biosensor and sensor chip development

Polymer chemistry

Microfluidics

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Interface Engineering with Self‐Assembled Monolayers in Biosensors

DNA interaction analysis of fluorenylidene double bridged cyclotriphosphazene derivatives

Inorganica Chimica Acta, 2018

DNA is a target molecule for anticancer drug development, and investigation on DNA interactions w... more DNA is a target molecule for anticancer drug development, and investigation on DNA interactions with synthesized potential drugs has great importance in order to discover new drug candidates, hence in this study an electrochemical biosensor has been used to perform quantitative analysis of DNA/compound interactions. In this study, full paraben substituted fluorenylidene double bridged cyclotriphosphazene compounds (9-13) were synthesized for the first time and their effect on DNA were investigated using an automated biosensor device MiSens Ò. The reaction of fluorenylidene double bridged cyclotriphospazene (FDBC) compound (3) with parabens: methyl 4-hydroxybenzoate, (4); ethyl 4-hydroxybenzoate, (5); propyl 4-hydroxybenzoate, (6); butyl 4-hydroxybenzoate (7), benzyl paraben (8) were performed, respectively. Compounds 9-13 were obtained and fully characterizated by mass spectrometry, 31 P NMR and 1 H spectroscopy. Biosensor based screening tests were used to investigate DNA interaction properties of the newly synthesised compounds by measuring DNA hybridization efficiency on the biochip surface. The effect of the compounds to the plasmid DNA structure were analyzed by agarose gel electrophoresis, in addition the antimicrobial activities of the compounds have been investigated. The biosensor assay and the agarose gel electrophoresis investigation indicated DNA/compound interaction for the compounds 12 and 13 none of the compounds has shown antimicrobial activity.

Yildiz Uludag

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