Skip to main content

M. Sadigursky

University of Bahia, Gonã §Alomoniz Research, Faculty Member

Followers

15

Following

18

Co-authors

7

Mentions

1,508

Public Views

Professor of immunology Universidade Federal da Bahia-Brasil

less

Interests

Uploads

Papers by M. Sadigursky

A biocompatibilidade dos materiais pode ser avaliada através dos mastócitos?

Revista de Ciências Médicas e Biológicas, 2008

O objetivo do trabalho foi estudar a participação dos mastócitos nos processos de cura de feridas... more O objetivo do trabalho foi estudar a participação dos mastócitos nos processos de cura de feridas, considerando a sua presença na composição dos critérios de avaliação e interpretação das respostas teciduais de biocompatibilidade. A atividade experimental foi realizada usando-se quarenta Rattus norvegicus albinus, divididos aleatoriamente em quatro grupos (1, 2, 3 e 4) para avaliação em vinte e quatro horas, uma, duas e três semanas, respectivamente. Cada animal serviu para os grupos experimentais (região dorsocefálica), onde foi implantada uma membrana biocompatível no tecido conjuntivo subcutâneo e controles sham (região dorsocaudal), onde o tecido conjuntivo foi somente manuseado. Os resultados evidenciaram que, nos grupos experimentais, a quantidade de mastócitos nos tecidos foi menor do que nos respectivos grupos sham. Entretanto, a comparação do número dessas células não revelou significância estatística. Os valores estatísticos encontrados para um valor de p=0,05, usando o te...

Holochilus brasiliensis nanus Thomas, 1897: sugestão de modelo experimental para filariose, leishmaniose e esquistossomose

by M. Sadigursky and Reginaldo P Brazil

Revista do Instituto de Medicina Tropical de São Paulo, 1984

Failure to find anti-component 5 antibodies in animals infected with Trypanosoma cruzi

Transactions of the Royal Society of Tropical Medicine and Hygiene, 1987

Absence of schistosomal glomerulopathy in Schistosoma haematobium infection in man

Transactions of the Royal Society of Tropical Medicine and Hygiene, 1976

Kidneys were studied by light microscopy in 246 consecutive autopsies in Cairo, Egypt. Glomerulon... more Kidneys were studied by light microscopy in 246 consecutive autopsies in Cairo, Egypt. Glomerulonephritis was not related to the presence or intensity of either S. haematobium or S. mansoni infection, and mesangial thickening and proliferation were also unrelated to schistosome infection. Acute and chronic pyelonephritis were also unrelated to the presence of schistosome infection.

Immunofluorescence studies of schistosome structures which share determinants with circulating schistosome antigens

Transactions of the Royal Society of Tropical Medicine and Hygiene, 1978

An Infant with down Syndrome and Fever of Unknown Origin

Pediatric Annals, 2010

A 4-month-old boy with Down syndrome was admitted with fever for the past 2 months. The only addi... more A 4-month-old boy with Down syndrome was admitted with fever for the past 2 months. The only additional complaint was respiratory distress when he was fed. He received amoxicillin, amoxicillin-clavulanate, ceftriaxone, and cefepime during four previous hospitalizations without improvement. He was transferred to our children's hospital to investigate fever of unknown origin. A Calmette-Guérin bacillus (BCG) vaccine was given at 50 days. His mother was HIV-negative, reported cough for more than 2 weeks, and close contact during pregnancy with a patient who died with contagious pulmonary tuberculosis. The mother's tuberculin skin test (PPD) was 10 mm. On admission, the patient's peripheral blood examination showed a white blood cell count of 7.7 X 10 9 /L, with 64.2% neutrophils, 23.6% lymphocytes (1.8 x 10 9 /L absolute count), 11.4% monocytes, 0.7% basophils, and 0.1% eosinophils.

Development of metacyclic Leishmania promastigotes is associated with the increasing expression of GP65, the major surface antigen

Parasite Immunology, 1989

Using immunofluorescence techniques and flow microfluorometry analysis, we have demonstrated that... more Using immunofluorescence techniques and flow microfluorometry analysis, we have demonstrated that the binding of a monoclonal antibody (VD5/25) produced against GP65, the major surface antigen of Leishmania braziliensis, increased on the surface of stationary-phase promastigotes from all the New World Leishmania species causing mucocutaneous or cutaneous disease as compared with the log-phase parasites. In addition, a sequential development of Leishmania amazonensis promastigotes from a non-infective to an infective stage was demonstrated. Indeed, promastigotes in the stationary phase (days 6-7) were found to be far more infective than those in the logarithmic phase of growth (day 3) both in vitro for mouse peritoneal macrophages and in vivo for BALB/c mice. The intracellular survival and multiplication of L. amazonensis were significantly inhibited when infective promastigotes were treated with the VD5/25 monoclonal antibody. The increasing expression of GP65 on the promastigote surface may thus contribute to Leishmania infectivity. This seems to represent a characteristic mechanism applicable to all New World Leishmania species studied.

Evaluation of factors that can modify platelet-rich plasma properties

Oral Surgery, Oral Medicine, Oral Pathology, Oral Radiology, and Endodontology, 2008

Objective The aim of this article is to discuss a protocol for obtaining platelet-rich plasma (PR... more Objective The aim of this article is to discuss a protocol for obtaining platelet-rich plasma (PRP) and evaluate which factors, derived from its preparation method and from whole blood, modify PRP cytometry and coagulation time. Study design Whole blood, harvested from 50 rabbits, was centrifuged at 300g for 10 minutes. Supernatant was recentrifuged at 5000g for 5 minutes. PRP was clotted with calcium chloride. Whole blood and PRP cytometry were obtained through automatic measurement. The amount of erythrocyte-and platelet-poor plasma drawn from whole blood was measured. Hematocrit, platelet and leukocyte count, mean corpuscular volume (MCV) and mean platelet volume (MPV), mean, standard deviation, and median were also calculated at whole blood and PRP. PRP coagulation time was also analyzed. Mean values between groups were analyzed using Student t test. Correlations were evaluated using Pearson's correlation coefficient. The significance level was set at P Ͻ .05. A linear regression was performed to investigate the relationship among the correlated variables. Results From whole blood, 2.68 mL of erythrocytes and 5.72 mL of platelet-poor plasma (PPP) were removed. PRP platelet count was 2,324,080 cells/L. Whole blood hematocrit influenced the amount of cells and PPP removed, as well as PRP platelet count. PRP platelet count was dependent on whole blood hematocrit and platelet count, and does not interfere in PRP coagulation time. A linear interaction was confirmed between the variables that presented significant Pearson correlation. Conclusions The protocol evaluated produces a good PRP. Whole-blood parameters can predict PRP features. Wholeblood hematocrit is an important variable for PRP preparation and PRP cytometry characterization. PRP platelet count is dependent upon whole-blood platelet count.

Complementary surface epitopes, myotropic adhesion and active grip in Trypanosoma cruzi-host cell recognition

Molecular and Biochemical Parasitology, 1988

Plasma membranes with complementary surface epitopes and with essentially the same orientation as... more Plasma membranes with complementary surface epitopes and with essentially the same orientation as tissue infective metacyclic trypomastigotes and epimastigotes of Trypanosoma cruzi adhere to L6 myoblast host cells preferentially to smooth muscle and epithelial cells as a function of time, surface area and concentration in saturation phenomena at 4 and 37 degrees C. The initial adhesion rates are partially calcium ion dependent and, at saturation, they are also dependent on high energy phosphorylated intermediates, exerting an active grip on adherent parasite membranes. These phenomena are consistent with the existence of parasite attachment molecules on the external surface of the plasma membrane and complementary host cell receptor structures with the capacity to bind them.

The subclinical form of experimental visceral leishmaniasis in dogs

Memórias do Instituto Oswaldo Cruz, 1993

Evaluation of spleen cell population and effect of splenectomy on granuloma modulation in BALB/c mice infected with Schistosoma mansoni

Memórias do Instituto Oswaldo Cruz, 1993

A kinetic study of the cells present in the spleen of BALB/c mice infected with Schistosoma manso... more A kinetic study of the cells present in the spleen of BALB/c mice infected with Schistosoma mansoni was carried out. The lymphocytes were evaluated phenotypically with monoclonal antibodies and the effect of splenectomy on the modulation of periovular granuloma was also investigated. The infected mice had proportional increases in the numbers of neutrophils, plasma cells, macrophages and eosinophils in the spleen. The largest number of neutrophil, plasma cells and macrophage were found between the 8th and the 12th week of infection, while the amount of eosinophils were higher later on, around the 20th week. The lymphocytes phenotypically characterized as Thy 1.2, Lyt 1.2 (CD4) increased mildly in proportional numbers. However, the percentage of lymphocytes with the Lyt 2.2 (CD8) phenotype, which is characteristic of suppressor and cytotoxic T cells, increased significantly with the progress of the disease. The numbers of B lymphocytes, which comprise 50% of the mononuclear cells present in the spleen, increased significantly till the 16th week when they began to decrease. The mean diameters of periovular granulomas were comparatively similar in both experimental groups (splenectomized and non-splenectomized mice). However, the evolutive types of granuloma (exudative, intermediate and fibrous) in splenectomized mice were proportionally different from those of non splenectomized mice in the 16th and 24th week of infection. It is inferred that lymphnodes or other secondary lymphoid organs, in the absence of the spleen, assume a modulating action on periovular granulomas, although the evolution of the granulomas is somehow delayed in splenectomized mice.

Evaluation of Chagas' disease transmission through breast-feeding

Memórias do Instituto Oswaldo Cruz, 1988

One hundred milk or colostrum samples from 78 mothers with chronic Chagas&#39; disease were p... more One hundred milk or colostrum samples from 78 mothers with chronic Chagas&#39; disease were parasitologically studied for Trypanosoma cruzi infection by means of direct examination and inoculation of mice. The mice were submitted to direct blood examination three times a week. At the end of 45 days, xenodiagnosis and indirect immunofluorescent test (IFAT) for T. cruzi antibodies were carried out in the animals. No parasitized sample was observed even though five mothers had parasitemia at milk collection. In addition, 97 breast-fed children of chronic chagasic mothers, born free of infection, were tested for IgG antibodies to T. cruzi using IFAT. No case of T. cruzi infection was detected. The authors conclude that breast-feeding should not be avoided for children of chronic chagasic women. However, as these mothers had intermittent parasitemia, they should avoid nursing when there is nipple bleeding.

Natural infection of the opossum Didelphis albiventris (Marsupialia, Didelphidae) with Leishmania donovani, in Brazil

Memórias do Instituto Oswaldo Cruz, 1984

Immunopathology of Schistosoma mansoni infection in rabbits: (A preliminary report)

Memórias do Instituto Oswaldo Cruz, 1982

Evolution on the immunopathology of Chagas disease

Memórias do Instituto Oswaldo Cruz, 1999

Induced tolerance to Schistosoma mansoni antigens modulates periovular granuloma

by Maria Falangola and M. Sadigursky

Memórias do Instituto Oswaldo Cruz, 1987

A Man With Newly Diagnosed HIV/AIDS With Unusual Severe Opportunistic Infection and No AIDS-Defining Disease

by M. Sadigursky, Roberto Badaró, and Marcos Figueredo

Clinical Infectious Diseases, 2014

Experimental infection of the Opossum didelphis albiventris (Marsupialia, didelphidae) with Leishmania donovani

Memórias do Instituto Oswaldo Cruz, 1988

Infectivity of Leishmania promastigotes is associated with surface antigenic expression

Immunology Letters, 1985

Differentiation between a non-infective and an infective Leishmania promastigote population was d... more Differentiation between a non-infective and an infective Leishmania promastigote population was demonstrated. Promastigotes in the stationary phase (day 5) were found to be highly infective in vitro to BALB/c mouse peritoneal macrophages, compared with those of the logarithmic phase (day 3). The infective promastigotes showed surface antigenic determinants different from non-infective ones. Polyclonal anti-3 day and anti-5 day antibodies were bound specifically to the surface of corresponding promastigotes in both SRIA and IFAT; no strong cross-reactions were observed otherwise. Also, polyclonal anti-5 day but not anti-3 day antibodies recognized efficiently the antigenic molecules on the surface of late stage (day 7) sandfly promastigotes. This clearly indicates the appearance of new antigenic molecules on the surface of infective promastigote forms. Intracellular multiplication of Leishmania was significantly inhibited by anti-5 day antibodies compared with anti-3 day antibodies. The presence of new surface molecules on late stage promastigotes may contribute to Leishmania infectivity.

Anti-Striated Muscle Antibody Activity Produced by Trypanosoma cruzi

Experimental Biology and Medicine, 1983

We have previously shown that Trypanosoma cruzi shares antigenic determinants with preparations o... more We have previously shown that Trypanosoma cruzi shares antigenic determinants with preparations of the calcium-sequestering adenosine triphosphatase of sarcoplasmic reticulum. The cross-reacting antigen (SRA) is also apparently present on the sarcolemma of cardiac myofibers. Using highly specific reference antisera to either the small membranes of T. cruzi or to a tryptic fragment of striated muscle SRA, it was shown that SRA is present in the striated muscle of animals representative of the evolutionary scale ranging from nonhuman primate to fish. The small membranes of nine different T. cruzi strains isolated from widely divergent areas of the American continents also reacted with the reference antisera. This indicates that SRA is present in these T. cruzi strains and may be prevalent among all T. cruzi strains. The shared T. cruzi-striated muscle antigen, SRA, may be a heteroantigen present in all T. cruzi strains and in the striated muscle of all classes of animals. Immunization of rabbits (three of five) or chickens (five pairs of five pairs) with striated muscle membrane preparations of different classes of animals, particularly those of nonhuman primate, chicken, and turtle, gave rise to IgG anti-allogeneic striated muscle antibody activity. Immunization of rabbits (four of nine) and chickens (five pairs of six pairs) with the small membranes of different T. cruzi strains also produced IgG anti-allogeneic striated muscle. These data indicate that T. cruzi shares cross-immunogenicity with striated muscle SRA. Since SRA is apparently present on the sarcolemma of cardiac myofibers, it may be implicated in the immunopathogenesis of Chagas&#39; disease.

A biocompatibilidade dos materiais pode ser avaliada através dos mastócitos?

Revista de Ciências Médicas e Biológicas, 2008

O objetivo do trabalho foi estudar a participação dos mastócitos nos processos de cura de feridas... more O objetivo do trabalho foi estudar a participação dos mastócitos nos processos de cura de feridas, considerando a sua presença na composição dos critérios de avaliação e interpretação das respostas teciduais de biocompatibilidade. A atividade experimental foi realizada usando-se quarenta Rattus norvegicus albinus, divididos aleatoriamente em quatro grupos (1, 2, 3 e 4) para avaliação em vinte e quatro horas, uma, duas e três semanas, respectivamente. Cada animal serviu para os grupos experimentais (região dorsocefálica), onde foi implantada uma membrana biocompatível no tecido conjuntivo subcutâneo e controles sham (região dorsocaudal), onde o tecido conjuntivo foi somente manuseado. Os resultados evidenciaram que, nos grupos experimentais, a quantidade de mastócitos nos tecidos foi menor do que nos respectivos grupos sham. Entretanto, a comparação do número dessas células não revelou significância estatística. Os valores estatísticos encontrados para um valor de p=0,05, usando o te...

Holochilus brasiliensis nanus Thomas, 1897: sugestão de modelo experimental para filariose, leishmaniose e esquistossomose

by M. Sadigursky and Reginaldo P Brazil

Revista do Instituto de Medicina Tropical de São Paulo, 1984

Failure to find anti-component 5 antibodies in animals infected with Trypanosoma cruzi

Transactions of the Royal Society of Tropical Medicine and Hygiene, 1987

Absence of schistosomal glomerulopathy in Schistosoma haematobium infection in man

Transactions of the Royal Society of Tropical Medicine and Hygiene, 1976

Kidneys were studied by light microscopy in 246 consecutive autopsies in Cairo, Egypt. Glomerulon... more Kidneys were studied by light microscopy in 246 consecutive autopsies in Cairo, Egypt. Glomerulonephritis was not related to the presence or intensity of either S. haematobium or S. mansoni infection, and mesangial thickening and proliferation were also unrelated to schistosome infection. Acute and chronic pyelonephritis were also unrelated to the presence of schistosome infection.

Immunofluorescence studies of schistosome structures which share determinants with circulating schistosome antigens

Transactions of the Royal Society of Tropical Medicine and Hygiene, 1978

An Infant with down Syndrome and Fever of Unknown Origin

Pediatric Annals, 2010

A 4-month-old boy with Down syndrome was admitted with fever for the past 2 months. The only addi... more A 4-month-old boy with Down syndrome was admitted with fever for the past 2 months. The only additional complaint was respiratory distress when he was fed. He received amoxicillin, amoxicillin-clavulanate, ceftriaxone, and cefepime during four previous hospitalizations without improvement. He was transferred to our children's hospital to investigate fever of unknown origin. A Calmette-Guérin bacillus (BCG) vaccine was given at 50 days. His mother was HIV-negative, reported cough for more than 2 weeks, and close contact during pregnancy with a patient who died with contagious pulmonary tuberculosis. The mother's tuberculin skin test (PPD) was 10 mm. On admission, the patient's peripheral blood examination showed a white blood cell count of 7.7 X 10 9 /L, with 64.2% neutrophils, 23.6% lymphocytes (1.8 x 10 9 /L absolute count), 11.4% monocytes, 0.7% basophils, and 0.1% eosinophils.

Development of metacyclic Leishmania promastigotes is associated with the increasing expression of GP65, the major surface antigen

Parasite Immunology, 1989

Using immunofluorescence techniques and flow microfluorometry analysis, we have demonstrated that... more Using immunofluorescence techniques and flow microfluorometry analysis, we have demonstrated that the binding of a monoclonal antibody (VD5/25) produced against GP65, the major surface antigen of Leishmania braziliensis, increased on the surface of stationary-phase promastigotes from all the New World Leishmania species causing mucocutaneous or cutaneous disease as compared with the log-phase parasites. In addition, a sequential development of Leishmania amazonensis promastigotes from a non-infective to an infective stage was demonstrated. Indeed, promastigotes in the stationary phase (days 6-7) were found to be far more infective than those in the logarithmic phase of growth (day 3) both in vitro for mouse peritoneal macrophages and in vivo for BALB/c mice. The intracellular survival and multiplication of L. amazonensis were significantly inhibited when infective promastigotes were treated with the VD5/25 monoclonal antibody. The increasing expression of GP65 on the promastigote surface may thus contribute to Leishmania infectivity. This seems to represent a characteristic mechanism applicable to all New World Leishmania species studied.

Evaluation of factors that can modify platelet-rich plasma properties

Oral Surgery, Oral Medicine, Oral Pathology, Oral Radiology, and Endodontology, 2008

Objective The aim of this article is to discuss a protocol for obtaining platelet-rich plasma (PR... more Objective The aim of this article is to discuss a protocol for obtaining platelet-rich plasma (PRP) and evaluate which factors, derived from its preparation method and from whole blood, modify PRP cytometry and coagulation time. Study design Whole blood, harvested from 50 rabbits, was centrifuged at 300g for 10 minutes. Supernatant was recentrifuged at 5000g for 5 minutes. PRP was clotted with calcium chloride. Whole blood and PRP cytometry were obtained through automatic measurement. The amount of erythrocyte-and platelet-poor plasma drawn from whole blood was measured. Hematocrit, platelet and leukocyte count, mean corpuscular volume (MCV) and mean platelet volume (MPV), mean, standard deviation, and median were also calculated at whole blood and PRP. PRP coagulation time was also analyzed. Mean values between groups were analyzed using Student t test. Correlations were evaluated using Pearson's correlation coefficient. The significance level was set at P Ͻ .05. A linear regression was performed to investigate the relationship among the correlated variables. Results From whole blood, 2.68 mL of erythrocytes and 5.72 mL of platelet-poor plasma (PPP) were removed. PRP platelet count was 2,324,080 cells/L. Whole blood hematocrit influenced the amount of cells and PPP removed, as well as PRP platelet count. PRP platelet count was dependent on whole blood hematocrit and platelet count, and does not interfere in PRP coagulation time. A linear interaction was confirmed between the variables that presented significant Pearson correlation. Conclusions The protocol evaluated produces a good PRP. Whole-blood parameters can predict PRP features. Wholeblood hematocrit is an important variable for PRP preparation and PRP cytometry characterization. PRP platelet count is dependent upon whole-blood platelet count.

Complementary surface epitopes, myotropic adhesion and active grip in Trypanosoma cruzi-host cell recognition

Molecular and Biochemical Parasitology, 1988

Plasma membranes with complementary surface epitopes and with essentially the same orientation as... more Plasma membranes with complementary surface epitopes and with essentially the same orientation as tissue infective metacyclic trypomastigotes and epimastigotes of Trypanosoma cruzi adhere to L6 myoblast host cells preferentially to smooth muscle and epithelial cells as a function of time, surface area and concentration in saturation phenomena at 4 and 37 degrees C. The initial adhesion rates are partially calcium ion dependent and, at saturation, they are also dependent on high energy phosphorylated intermediates, exerting an active grip on adherent parasite membranes. These phenomena are consistent with the existence of parasite attachment molecules on the external surface of the plasma membrane and complementary host cell receptor structures with the capacity to bind them.

The subclinical form of experimental visceral leishmaniasis in dogs

Memórias do Instituto Oswaldo Cruz, 1993

Evaluation of spleen cell population and effect of splenectomy on granuloma modulation in BALB/c mice infected with Schistosoma mansoni

Memórias do Instituto Oswaldo Cruz, 1993

A kinetic study of the cells present in the spleen of BALB/c mice infected with Schistosoma manso... more A kinetic study of the cells present in the spleen of BALB/c mice infected with Schistosoma mansoni was carried out. The lymphocytes were evaluated phenotypically with monoclonal antibodies and the effect of splenectomy on the modulation of periovular granuloma was also investigated. The infected mice had proportional increases in the numbers of neutrophils, plasma cells, macrophages and eosinophils in the spleen. The largest number of neutrophil, plasma cells and macrophage were found between the 8th and the 12th week of infection, while the amount of eosinophils were higher later on, around the 20th week. The lymphocytes phenotypically characterized as Thy 1.2, Lyt 1.2 (CD4) increased mildly in proportional numbers. However, the percentage of lymphocytes with the Lyt 2.2 (CD8) phenotype, which is characteristic of suppressor and cytotoxic T cells, increased significantly with the progress of the disease. The numbers of B lymphocytes, which comprise 50% of the mononuclear cells present in the spleen, increased significantly till the 16th week when they began to decrease. The mean diameters of periovular granulomas were comparatively similar in both experimental groups (splenectomized and non-splenectomized mice). However, the evolutive types of granuloma (exudative, intermediate and fibrous) in splenectomized mice were proportionally different from those of non splenectomized mice in the 16th and 24th week of infection. It is inferred that lymphnodes or other secondary lymphoid organs, in the absence of the spleen, assume a modulating action on periovular granulomas, although the evolution of the granulomas is somehow delayed in splenectomized mice.

Evaluation of Chagas' disease transmission through breast-feeding

Memórias do Instituto Oswaldo Cruz, 1988

One hundred milk or colostrum samples from 78 mothers with chronic Chagas&#39; disease were p... more One hundred milk or colostrum samples from 78 mothers with chronic Chagas&#39; disease were parasitologically studied for Trypanosoma cruzi infection by means of direct examination and inoculation of mice. The mice were submitted to direct blood examination three times a week. At the end of 45 days, xenodiagnosis and indirect immunofluorescent test (IFAT) for T. cruzi antibodies were carried out in the animals. No parasitized sample was observed even though five mothers had parasitemia at milk collection. In addition, 97 breast-fed children of chronic chagasic mothers, born free of infection, were tested for IgG antibodies to T. cruzi using IFAT. No case of T. cruzi infection was detected. The authors conclude that breast-feeding should not be avoided for children of chronic chagasic women. However, as these mothers had intermittent parasitemia, they should avoid nursing when there is nipple bleeding.

Natural infection of the opossum Didelphis albiventris (Marsupialia, Didelphidae) with Leishmania donovani, in Brazil

Memórias do Instituto Oswaldo Cruz, 1984

Immunopathology of Schistosoma mansoni infection in rabbits: (A preliminary report)

Memórias do Instituto Oswaldo Cruz, 1982

Evolution on the immunopathology of Chagas disease

Memórias do Instituto Oswaldo Cruz, 1999

Induced tolerance to Schistosoma mansoni antigens modulates periovular granuloma

by Maria Falangola and M. Sadigursky

Memórias do Instituto Oswaldo Cruz, 1987

A Man With Newly Diagnosed HIV/AIDS With Unusual Severe Opportunistic Infection and No AIDS-Defining Disease

by M. Sadigursky, Roberto Badaró, and Marcos Figueredo

Clinical Infectious Diseases, 2014

Experimental infection of the Opossum didelphis albiventris (Marsupialia, didelphidae) with Leishmania donovani

Memórias do Instituto Oswaldo Cruz, 1988

Infectivity of Leishmania promastigotes is associated with surface antigenic expression

Immunology Letters, 1985

Differentiation between a non-infective and an infective Leishmania promastigote population was d... more Differentiation between a non-infective and an infective Leishmania promastigote population was demonstrated. Promastigotes in the stationary phase (day 5) were found to be highly infective in vitro to BALB/c mouse peritoneal macrophages, compared with those of the logarithmic phase (day 3). The infective promastigotes showed surface antigenic determinants different from non-infective ones. Polyclonal anti-3 day and anti-5 day antibodies were bound specifically to the surface of corresponding promastigotes in both SRIA and IFAT; no strong cross-reactions were observed otherwise. Also, polyclonal anti-5 day but not anti-3 day antibodies recognized efficiently the antigenic molecules on the surface of late stage (day 7) sandfly promastigotes. This clearly indicates the appearance of new antigenic molecules on the surface of infective promastigote forms. Intracellular multiplication of Leishmania was significantly inhibited by anti-5 day antibodies compared with anti-3 day antibodies. The presence of new surface molecules on late stage promastigotes may contribute to Leishmania infectivity.

Anti-Striated Muscle Antibody Activity Produced by Trypanosoma cruzi

Experimental Biology and Medicine, 1983

We have previously shown that Trypanosoma cruzi shares antigenic determinants with preparations o... more We have previously shown that Trypanosoma cruzi shares antigenic determinants with preparations of the calcium-sequestering adenosine triphosphatase of sarcoplasmic reticulum. The cross-reacting antigen (SRA) is also apparently present on the sarcolemma of cardiac myofibers. Using highly specific reference antisera to either the small membranes of T. cruzi or to a tryptic fragment of striated muscle SRA, it was shown that SRA is present in the striated muscle of animals representative of the evolutionary scale ranging from nonhuman primate to fish. The small membranes of nine different T. cruzi strains isolated from widely divergent areas of the American continents also reacted with the reference antisera. This indicates that SRA is present in these T. cruzi strains and may be prevalent among all T. cruzi strains. The shared T. cruzi-striated muscle antigen, SRA, may be a heteroantigen present in all T. cruzi strains and in the striated muscle of all classes of animals. Immunization of rabbits (three of five) or chickens (five pairs of five pairs) with striated muscle membrane preparations of different classes of animals, particularly those of nonhuman primate, chicken, and turtle, gave rise to IgG anti-allogeneic striated muscle antibody activity. Immunization of rabbits (four of nine) and chickens (five pairs of six pairs) with the small membranes of different T. cruzi strains also produced IgG anti-allogeneic striated muscle. These data indicate that T. cruzi shares cross-immunogenicity with striated muscle SRA. Since SRA is apparently present on the sarcolemma of cardiac myofibers, it may be implicated in the immunopathogenesis of Chagas&#39; disease.