Papers by magdi eldamarawi
Bulletin of Egyptian Society for Physiological Sciences
Background: Hepatic ischemia-reperfusion injury (IRI) can be mainly caused by oxidative stress, d... more Background: Hepatic ischemia-reperfusion injury (IRI) can be mainly caused by oxidative stress, decreased nitric oxide (NO) level, activation of hepatic Kupffer cells, neutrophils adhesions, increased level of intercellular adhesion molecules, and inflammatory process. Aim: Studying the ability of L-carnitine (LC) and L-arginine (LG) to protect the liver cells against the damage caused by IRI. Methods: This study was carried out on 40 male Wistar albino rats which were divided into 5 groups; shamoperated, hepatic IRI, IRI rats pretreated with L-carnitine, IRI rats pretreated with Larginine, IRI rats pretreated with both substances. Results: The pretreatment with LC and LG separately protected the liver cells against IRI damage with preservation of liver functions by significantly improving the oxidative stress and inflammatory states, increasing NO level, decreasing the expression of vascular adhesion molecules in liver tissue, and protecting the liver cells from the damage. It was obvious that LC effects were more significant on all the tested parameters except on the NO level where the LG effect was more significant. Moreover, the combined use of both LC and LG produced a highly significant improvement of all the tested parameters approaching near the control level than that observed by their separate use. Conclusion: The combined use of both LC and LG could effectively protect the liver cells from the harmful effects of IRI.
Background: Osteoarthritis (OA) is a common joint disease influencing the majority of individuals... more Background: Osteoarthritis (OA) is a common joint disease influencing the majority of individuals over the age of 65. The most affected joints in OA are knees, hands, hips, and spine leading to impaired mobility in the elderly. Objectives: To analyze and correlate the level of plasma adiponectin and the adiponectin G/T (rs1501299) single nucleotide polymorphism in a group of Egyptians with knee osteoarthritis (OA) and a control group. Materials and methods: 130 unrelated subjects with knee OA and 120 healthy subjects were enrolled in this study. Peripheral blood was used for extraction of DNA which was used to genotype adiponectin gene G/T (rs1501299) polymorphism utilizing polymerase chain reaction followed by digestion with restriction endonuclease. Plasma adiponectin level was estimated using enzyme linked immunosorbent assay (ELISA). Results: Plasma adiponectin concentration was higher in the OA subjects than the controls (P = 0.01). For the adiponectin G/T (rs1501299) polymorph...
Open Access Macedonian Journal of Medical Sciences, 2020
BACKGROUND: Uncoupling proteins (UCP) and β3-adrenergic receptor (ADRB3) gene polymorphism have b... more BACKGROUND: Uncoupling proteins (UCP) and β3-adrenergic receptor (ADRB3) gene polymorphism have been implicated in the susceptibility to type 2 diabetes mellitus (T2DM) but the results are inconsistent and inconclusive. AIM: The aim was to investigate the relationship between -3826A/G (rs1800592) of UCP 1 and ADRB3 Trp64Arg (C/T) (rs4994) gene polymorphism and the incidence of T2DM among Saudis. METHODS: Genotyping of both UCP1 and ADRB3 genes was carried out in 110 healthy controls and 108 unrelated Saudis with T2DM using polymerase chain reaction-based restriction fragment length polymorphism. RESULTS: The genotype and allele frequencies of the UCP1 gene did not differ significantly between diabetic subjects and controls (p > 0.05). However, the CT genotype and the T allele of ADRB3 were higher in diabetic subjects compared with the controls while the CC genotype and C allele were higher in the controls relative to the diabetic subjects (Odds ratios [OR]: 7.85, 95% confidence i...
Bulletin of Egyptian Society for Physiological Sciences, 2020
Background: Defects in glucose transporter-4 (GLUT-4) expression and function in the skeletal mus... more Background: Defects in glucose transporter-4 (GLUT-4) expression and function in the skeletal muscles and adipose tissue can be considered as the main cause of insulin resistance (IR) in type 2 diabetes mellitus (T2DM). Also, Oxidative stress and inflammation play a very important role in the development of IR and T2DM. Aim: Studying the effect of quercetin and metformin on GLUT-4 expression, oxidative stress, inflammation markers and IR in T2DM.Method: This study was carried out on 50 male Wistar rats which were divided into five groups; control, untreated diabetic rats, diabetic rats treated with metformin, diabetic rats treated with quercetin and diabetic rats treated with metformin and quercetin. Results: The use of metformin and quercetin separately produced significant decrease in plasma glucose, insulin, IL-6, TNF-α levels, HOMA-IR and TBARS level in skeletal muscle. Also, they caused significant elevation in the antioxidant enzyme activities in skeletal muscles with increased expression of GLUT-4 in the skeletal muscles and adipose tissue compared to the diabetic rats. Evidently, the quercetin effects were more significant than that of metformin on all the parameters except on HOMA-IR (similar significant improvement). Moreover, the combined use of quercetin and metformin produced highly significant improvement approaching control level in all the parameters than that observed by using either of them. Conclusion: The combined use of QC and MF improved hyperglycemia and IR by increasing the expression of GLUT-4 in skeletal muscles and adipose tissue together with the reversal of the oxidative stress and inflammatory states.
Genetics and molecular research : GMR, 2016
Coronary artery disease (CAD) represents a leading cause of morbidity and mortality worldwide, an... more Coronary artery disease (CAD) represents a leading cause of morbidity and mortality worldwide, and genetic factors contribute to the development of this disease. We conducted a case-control study to assess the association between interleukin 17A (IL17A) rs2275913 and rs3748067 polymorphisms and development of CAD. A total of 372 CAD patients and 372 healthy controls were recruited in our investigation between January 2013 and December 2014. Genotyping of IL17A rs2275913 and rs3748067 was carried out using polymerase chain reaction combined with restriction fragment length polymorphism. Logistic regression analysis revealed that CC [odds ratio (OR) = 3.81, 95% confidence interval (CI) = 2.11-7.16] and TC+CC (OR = 1.54, 95%CI = 1.11-2.14) rs3748067 genotypes were associated with an increased risk of CAD compared to the TT variant. Individuals carrying the TC+CC genotype were more likely to have a higher risk of CAD if they were smokers, with an adjusted OR (and 95%CI) of 2.20 (1.31-3....
Life Science Journal
AIM: This study was designed to compare the impact of two different exercise protocols on the ser... more AIM: This study was designed to compare the impact of two different exercise protocols on the serum level of advanced glycation end products (AGEs) in type 2 diabetic rats. METHODS: This study was performed on 40 male albino rats, weighed 200-250 g. At the start of the study, 10 male rats were separated and used as a control group (group I). Induction of diabetes in another 30 rats was done by a single intravenous injection of 45 mg/kg streptozotocin (STZ). The diabetic rats were randomly divided into two groups: group II (sedentary group) (n = 10) and group III (exercised group) (n = 20). Then, the exercised group was further subdivided into two subgroups: IIIa (were subjected to chronic regular moderate exercise protocol for 8 weeks and IIIb faced another protocol in the form of irregular strenuous exercise for the same period. RESULTS: The data in group II (sedentary group) as compared to group I (control group), showed that the injection of streptozotocin resulted in significant increase in the fasting blood glucose, serum AGEs and insulin levels and HOMA-IR. Also, there was significant decrease in the extracellular superoxide dismutase (SOD) enzyme serum level. Meanwhile, in subgroup IIIa (as compared to group II), regular moderate exercise protocol in diabetic rats produced significant reduction in the fasting blood glucose, serum AGEs and insulin levels and HOMA-IR. At the same time, there was significant increase in serum level of superoxide dismutase. Lastly, in subgroup IIIb (as compared to group II), the irregular intense exercise protocol in diabetic rats caused significant reduction in fasting blood glucose but there was insignificant decrease in serum AGEs and insulin levels and HOMA-IR. This was associated with insignificant increase in the superoxide dismutase serum level. CONCLUSION: In type 2 diabetes, regular moderate exercise protocol is more valuable program to reduce serum level of advanced glycation end products than irregular severe one. This regular moderate protocol will be very helpful in the prevention of development of diabetic complications. [Mahmoud E. Salama, Magdi A. El-Damarawi and Afrah F. Salama. A Comparison between the Impact of Two Different Exercise Protocols on Advanced Glycation End Products in Type 2 Diabetic Rats.
AIM: This research was designed to study the effect of chronic administration of obestatin on ser... more AIM: This research was designed to study the effect of chronic administration of obestatin on serum testosterone level in obese male rats. METHODS: This study was performed on 40 male albino rats, weighed 200-250 grams. Ten rats were used as a control group (group I) and the rest were made obese by feeding them a hypercaloric diet for 15 weeks. The obese rats were randomly divided into two groups, group II in which the rats received the hypercaloric diet only and group III which is subdivided into two subgroups a and b. In group III, the obese rats are kept on the hypercaloric diet with intraperitoneal administration of obestatin in a dose of 16 nmol/kg/day for two and four weeks in group IIIa and IIIb respectively. RESULTS: By checking the results of the different groups we noticed that obesity, in group II, produced significant increase in the body weight of the rats associated with significant decrease in the serum testosterone level. In group IIIa, administration of obestatin fo...
Background/Aim: The increased level of advanced glycation end products (AGEs) in diabetic patient... more Background/Aim: The increased level of advanced glycation end products (AGEs) in diabetic patients is considered one of the most important factors responsible for the development of diabetic complications. This study was designed to compare the effect of regular moderate exercise and pyridoxamine on the serum level of advanced glycation products in type 2 diabetic rats. Material & methods: This study was performed on 40 male albino rats; weighed 200-250 g. Ten rats were separated and used as a control group (group I). The other 30 rats were prepared to develop diabetes mellitus by a single intravenous injection of 45 mg/kg streptozotocin (STZ). Three days after injection of streptozotocin, fasting blood glucose was determined and animals with fasting blood glucose level more than 200 mg/dl were considered to have diabetes. Diabetic rats were divided into three groups: group II (diabetic group), group III (exercise group) and group IV (pyridoxamine group). Each group consisted of 10 ...
Background/Aim: This work was done to study the combined effect of diet restriction and moderate ... more Background/Aim: This work was done to study the combined effect of diet restriction and moderate regular exercise on plasma adiponectin level and expression of its receptors (AdipoR1) in skeletal muscles and their role in the amelioration of metabolic disturbances associated with obesity and type 2 diabetes mellitus. Materials & Methods: The study included 50 male Wistar albino rats weighed 150- 200 g, divided into 5 groups: control (group I), obese-diabetic (group II), obese-diabetic with diet restriction (group III), obese-diabetic with exercise (group IV) and obese-diabetic with diet restriction and exercise (group V). At the end of the study, body weight was detected, retro-orbital blood samples were analyzed for blood glucose, plasma triglycerides (TG), total cholesterol (TC), plasma insulin and adiponectin levels and insulin resistance was assessed. Also, mRNA level of AdipoR1 in skeletal muscles was measured. RESULTS: Comparing the results of group II (obese diabetic) to that...
This study was designed to investigate the effects of combination of antioxidants (Vitamins C and... more This study was designed to investigate the effects of combination of antioxidants (Vitamins C and E) on endothelial inflammation biomarkers (proinflammatory cytokines and endothelial adhesion molecules) and oxidative stress in liver and kidney in streptozotocin-induced diabetic rats. Thirty male albino rats, weighing between 170 and 200 g were used. The animals were divided into three groups as follows; control non diabetic rats (group I), untreated diabetic rats (group II), and diabetic rats treated with vitamins C and E (group III). Diabetes was developed 3 days after intraperitoneal administration of a single 40 mg/kg dose of streptozotocin in groups II and III. Thereafter, the rats in group III began to receive a combination of vitamins C and E for four weeks. The rats in group II had significantly higher levels in plasma glucose, total cholesterol (TC), triglycerides (TG), interleukin-6 (IL-6), tumor necrosis factor-α (TNF-α), intercellular adhesion molecule-1 (ICAM-1) and vasc...
Tanta Medical Journal, 2014
ABSTRACT
Conventional oral spirometry is a commonly used test for respiratory functions. However, the nasa... more Conventional oral spirometry is a commonly used test for respiratory functions. However, the nasal passages are the primary pathway for regulating ventilation and modulating ventilated air. Here, we tested the validity of using the nasal route (nasal spirometry) for the evaluation of respiratory functions. 250 healthy young adults (150 males and 100 females; 17 to 23 years of age) were subjected to two spirometry tests: oral spirometry by using a mouth piece and nasal spirometry by using a face mask. Measurement parameters included: Vital capacity (VC), forced vital capacity (FVC), forced expiratory volume first second (FEV 1 ), FEV 1 /FVC%, Forced expiratory flow (FEF 25/75 %). and maximum voluntary ventilation (MVV). In both males and females, only VC was significantly higher in nasal than oral spirometry, while FVC, FEV 1 , FEV 1 /FVC % FEF 25/75 % and MVV were significantly higher in oral than nasal spirometry. Prediction equations for different measurements of nasal spirometry were derived by multiple regression analysis using sex, height, and weight as independent variables. We conclude that nasal spirometry could be a valid procedure which may be more real in expressing normal respiratory functions.
Open Access Macedonian Journal of Medical Sciences, 2014
Citation: Alsawy BM, El-Damarawi MA. Effect of Diabetes Mellitus and Its Control on Myocardial Co... more Citation: Alsawy BM, El-Damarawi MA. Effect of Diabetes Mellitus and Its Control on Myocardial Contractile Function in Rats. Maced J Med Sci. 2014 Sep 15; 7(3):431-438. http://dx.
Internet Journal of Internal Medicine
Background/Aim: Lung function testing is an important measure to diagnose many respiratory disord... more Background/Aim: Lung function testing is an important measure to diagnose many respiratory disorders.
Life Science Journal
Aim of the work: The present study was designed to assess the effect of inulin on metabolic chang... more Aim of the work: The present study was designed to assess the effect of inulin on metabolic changes produced by fructose rich diet. Methods: 45 male albino rats were divided into three groups (each group consisted of 15 rats); first (control) group fed standard commercial chow with tap water for 3 weeks, second (Fructose rich diet, FRD) group fed fructose rich diet in the dose 1.74 g / 100 g body weight per day by nasogastric tube plus standard commercial chow with tap water for 3 weeks and third (inulin treated) group fed fructose rich diet in the same dose and inulin in the dose of 0.174 g / 100 g body weight plus standard commercial chow with tap water for 3 weeks. Results: In the FRD group, the high fructose diet produced significant increase in blood level of glucose, insulin, and in insulin resistance. Also, the same group showed significant increase in serum level of total cholesterol, triglycerides (TG) and Low density lipoproteins (LDL) with significant decrease in High density lipoproteins (HDL) as compared to the control group. Inulin supplemented group showed significant decrease in blood levels of glucose, insulin and in insulin resistance. In addition, inulin supplementation caused significant elevation in the serum level of HDL with significant reduction in total cholesterol, TG and LDL serum levels as compared to FRD group. Conclusion: This study demonstrated that inulin could play a role in the correction of the metabolic disturbances produced by high fructose diet by improvement of carbohydrate and lipid metabolism.
AIM: This study was designed to compare the impact of two different exercise protocols on the ser... more AIM: This study was designed to compare the impact of two different exercise protocols on the serum level of advanced glycation end products (AGEs) in type 2 diabetic rats. METHODS: This study was performed on 40 male albino rats, weighed 200-250 g. At the start of the study, 10 male rats were separated and used as a control group (group I). Induction of diabetes in another 30 rats was done by a single intravenous injection of 45 mg/kg streptozotocin (STZ). The diabetic rats were randomly divided into two groups: group II (sedentary group) (n = 10) and group III (exercised group) (n = 20). Then, the exercised group was further subdivided into two subgroups: IIIa (were subjected to chronic regular moderate exercise protocol for 8 weeks and IIIb faced another protocol in the form of irregular strenuous exercise for the same period. RESULTS: The data in group II (sedentary group) as compared to group I (control group), showed that the injection of streptozotocin resulted in significant increase in the fasting blood glucose, serum AGEs and insulin levels and HOMA-IR. Also, there was significant decrease in the extracellular superoxide dismutase (SOD) enzyme serum level. Meanwhile, in subgroup IIIa (as compared to group II), regular moderate exercise protocol in diabetic rats produced significant reduction in the fasting blood glucose, serum AGEs and insulin levels and HOMA-IR. At the same time, there was significant increase in serum level of superoxide dismutase. Lastly, in subgroup IIIb (as compared to group II), the irregular intense exercise protocol in diabetic rats caused significant reduction in fasting blood glucose but there was insignificant decrease in serum AGEs and insulin levels and HOMA-IR. This was associated with insignificant increase in the superoxide dismutase serum level. CONCLUSION: In type 2 diabetes, regular moderate exercise protocol is more valuable program to reduce serum level of advanced glycation end products than irregular severe one. This regular moderate protocol will be very helpful in the prevention of development of diabetic complications. [Mahmoud E. Salama, Magdi A. El-Damarawi and Afrah F. Salama. A Comparison between the Impact of Two Different Exercise Protocols on Advanced Glycation End Products in Type 2 Diabetic Rats. Life Sci J 2013;10(3):860-869] (ISSN: 1097-8135). http://www.lifesciencesite.com. 129
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Papers by magdi eldamarawi