Papers by Dr.Subhasree C.R.
J Clin Bioanal Chem., 2022
Introduction: The present study is to investigate the total antioxidant activity (TAC) in suspect... more Introduction: The present study is to investigate the total antioxidant activity (TAC) in suspected neonates of inborn errors of metabolism with high risk, moderate risk and low risk. Dried blood spots have potential use in remote health applications for individual and population diagnosis and can enable epidemiological surveillance for known and unknown diseases. Dried blood spot sampling is most common method for collection, storing, transporting and analyzing a variety of human body fluids. Materials and methods: After performing, while newborn screening is early period, the balance residual samples were used for specific secondary research studies with patient consent. Here we are using the secondary studies of DBS for total antioxidant activity as one of the biomarkers to find out the disease condition. TAC was evaluated in 449 risk subjects and 531 control neonates including high, moderate, low risk and healthy individuals. The risk variables were included birth weight, APGAR score, gestational age and previous complications of pregnancy. DBS-TAC method, is to elute sample by using phosphate buffered saline. After sample preparation, TAC by using FRAP assay (ferric reducing antioxidant power assay) were estimated by among four groups with risk indicators. Results: Statistical comparisons and correlations at 5% level of significance were determined. The mean TAC concentration was significantly elevated in high risk group. The mean TAC for low, moderate and high-risk group (Mean ± SD values were 954.8 ± 132.2 μmol/L, 700.4 ± 100.6 μmol/L and 510.4 ± 110 μmol/L respectively) patients were significantly reduced compared with control group (Mean ± S.D. valued was 1017.3 ± 122.2 μmol/L) (p < 0.05). Likely the SOD, Vit C, Vit E and MDA also were estimated. Discussion: DBS-TAC can be used as early biochemical marker for oxidative stress in high risk for IEM (Inborn errors of metabolism) which may result in reduced tissue damage by free radicals and help to monitor and optimize antioxidant therapy in such high risk neonates. Conclusion: FRAP assay is presented as a novel method of assessing total antioxidant capacity and is considered as a useful indicator of the body's antioxidant status to counteract the oxidative damage due to ROS. The advantage of the FRAP assay is in being fast, easy to handle, with highly reproducible results. A significantly lower DBS TAC concentration in the IEM high risk patients compared to controls reflects a lower total antioxidant capacity. Thus, TAC may be useful as an early marker of oxidative stress to monitor and optimize antioxidant therapy as an adjunct in the management of IEM patients.
International journal of Contemporary pediatrics, 2023
Genetic condition is a methylmalonate and cobalamin (cbl; vitamin B12) metabolism genetically div... more Genetic condition is a methylmalonate and cobalamin (cbl; vitamin B12) metabolism genetically diverse condition. Isolated methylmalonic aciduria is observed in patients with partial, mut (-) or complete mut (0) mutations in the MUT gene producing enzyme deficiency. This form is just not B12 treatment responsive. A subset of patients with synthesis-default MUT coenzyme adenosylcobalamin (AdoCbl) are also affected by a number of forms of isolated methylmalonic aciduria and are classified in complementary groups: cblA (251100), caused by MMAA gene mutation (607481) at 4q31 chromosome, and cblB (251110), due to MMAB gene mutation (77568) at 12q24). 1-3 CASE REPORT The male newborn was born of a consanguineous marriage. His brain MRI brain showed high signal with ABSTRACT An uncommon autosomal recessive organic acid disease is malonic aciduria. This disease may be easily identified and included in the NBS programmes by means of the widespread use of tandem mass spectrometry's in the study of the amino acid/acylcarnitine profile using dried blood spots (DBS) for newborn screening. In Tamil Nadu, we reported the first screened and diagnosed with malonic aciduria by newborn screening (NBS) in early neonatal period. The patient possesses a malonyl-CoA decarboxylase genetic variation not previously described. This disease should be distinguished from a related malonic and methylmalonic aciduria problem. Malonic aciduria's clinical phenomenology varies and pathogenesis is not completely known. The proper treatment regimen, nutritional therapy or frequent follow-up to these individuals are not guided or recommended. The majority of current treatment data is based on a single research or case report.
Background: Thyroid hormones play an important role in human metabolism. Red blood cells abnormal... more Background: Thyroid hormones play an important role in human metabolism. Red blood cells abnormalities are mostly associated with thyroid hormones. However, they are very rarely investigated and associated to the permanent and transient congenital hypothyroidism in Tamil Nadu's Government Territory care centre in Chennai. In this study, an attempt was made to study the haematological status in permanent and transient hypothyroidism neonates at birth. Materials and Methods: This retrospective study included 135 subjects, among which were permanent hypothyroidism (n=9), transient hypothyroidism (n=18), and euthyroid neonates (108). This study was carried out at department of biochemistry, Regenix super speciality laboratories and sample collection were done at Government Institute of Child's health and hospital for children and Government institute of obstetrics and Gynaecology and hospital for Women under Madras Medical college, Chennai. The haematological parameters and thyroid profile of the subjects were assessed by sysmex heamatology analyser, Roche Cobas e411 ECLIA and Neonatal screening by BIORAD QUANTASE ELISA. Mean and standard deviation, analysis of variance (two way anova) with p<0.05 considered as statistically significant. Results: In this study group, we compared the homological status in these groups, permanent, transient, and euthyroid as controls neonates. We found that haematological status like Hb, RBC, MCV, and RDW were significantly increased at birth in both permanent and transient hypothyroidism were compared with euthyroid neonates, results are significant. The results reported in these are Mean ± S.D., were statistically tested by ANOVA. In permanent Congenital Hypothyroidism (CH), MCV (79.97-87.58 fl) and RDW (13.98-16.6%), whereas in Transient Congenital Hypothyroidism, MCV (72.52-87.05 fl) and RDW (13.41-15.68%), and controls MCV (78.69-82.12 fl) and RDW (12.4-14.3%), suggesting that these patients were at risk of anemia and other RBS abnormalities. Mean corpuscular volume is an important to view the change in RBCs destruction, production, loss and morphology. Conclusion: The thyroid dyshormonogenesis is frequently associated with mothers' complications like anemia, hypo/hyperthyroid mother, siblings developmental delay, and goitre. Permanent CH is associated with serious complication with erythrocyte abnormalities. The risk of congenital hypothyroidism cloud be getting into developmental delay and mentally retarded children, Such conditions should be detect early and corrected.
Paediatric Review:International journal of Paediatric Research, 2019
For many years, pre-analytical variables were only inappropriately considered in the field of dri... more For many years, pre-analytical variables were only inappropriately considered in the field of dried blood spots (DBS)testing and even today, with the exception of newborn screening, the entire pre-analytical phase, which comprises the
preparation and processing of DBS for their final analysis has not been standardized. (1) compared to conventional
venepuncture, less blood volume is required and this fact was most important in paediatric diagnostics; (2) blood
collection is simple, non-invasive, and inexpensive; (3) the risk of bacterial contamination or haemolysis is minimal; and
(4) DBS can be preserved for long periods with almost no deterioration of the analytes. Advances techniques applied to
diagnose the disorders of Inborn errors of metabolism. Dried blood spots (DBSs) have potential use in remote health
applications for individual and population diagnosis, and can enable epidemiological surveillance for known and
unknown diseases. DBS sampling offers great potential for remote health applications as a cost-effective sample format
for large-scale screening and diagnostic purposes, but the DBS samples often need to be transported from remote
sampling locations (e.g., tropical or developing countries) to analytical laboratories where the quantitative measurements
of different metabolic or disease biomarkers can be carried out. Dried blood spots are commonly method for collecting,
storing, transporting, and analysing a variety of human body fluids. Since 2006, however a variety of new and innovative
dried blood spots applications, although this pre-analytical approach represents an interesting alternative to classical
venous blood sampling, its routine use is limited. Here, we review the application of DBS technology in clinical
chemistry, molecular assays, small molecules, and large molecules all are perfectly suited to evaluate its future role
supported by new analytical methods such as mass spectrometry
Tuberculosis caused by multidrug-resistant (MDR) and extensively drug-resistant (XDR) Mycobacteri... more Tuberculosis caused by multidrug-resistant (MDR) and extensively drug-resistant (XDR) Mycobacterium tuberculosis (MTB) strains is a growing problem in many countries. The availability of the complete nucleotide sequences of several MTB genomes allows to use the comparative genomics as a tool to study the relationships of strains and differences in their evolutionary history including acquisition of drug-resistance. In our study, online resources for comparative genomics analysis between strains by using databases such as MGDD (Mycobacterial Genome Divergence Database), Biohealth base, GenomycDB, Mycobacterium tuberculosis database, and TBDB (Tuberculosis Database). Taken together, this study reveals that strain-specific variations in protein expression patterns have a meaningful impact on the biology of the pathogen.
Bifunctional dCTP deaminase – Deoxyuridine triphosphatase (dUTPase) from Mycobacterium tuberculos... more Bifunctional dCTP deaminase – Deoxyuridine triphosphatase (dUTPase) from Mycobacterium tuberculosis was selected as drug target from database DDTRP (Database of Drug Targets for Resistant Pathogens) that provides a list of potential drug target for different drug resistant infectious diseases including Tuberculosis (TB).The Deoxycytidine triphosphate deaminase ( dCTP) was docked with 131 molecules screened from four different databases, Pubchem, Drugbank, Zinc, and Chembank. Ninety three molecules were successfully docked with dCTP. Eight molecules, two from each database screened were suggested as better molecules over others to bind with dCTP. These eight molecules taken further studies to check their efficiency in vitro.
online) 0973-8894 (print) Bioinformation 7(2): 98-101 (2011) 98
Conference Presentations by Dr.Subhasree C.R.
Indian journal of clinical Biochemistry, 2019
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Papers by Dr.Subhasree C.R.
preparation and processing of DBS for their final analysis has not been standardized. (1) compared to conventional
venepuncture, less blood volume is required and this fact was most important in paediatric diagnostics; (2) blood
collection is simple, non-invasive, and inexpensive; (3) the risk of bacterial contamination or haemolysis is minimal; and
(4) DBS can be preserved for long periods with almost no deterioration of the analytes. Advances techniques applied to
diagnose the disorders of Inborn errors of metabolism. Dried blood spots (DBSs) have potential use in remote health
applications for individual and population diagnosis, and can enable epidemiological surveillance for known and
unknown diseases. DBS sampling offers great potential for remote health applications as a cost-effective sample format
for large-scale screening and diagnostic purposes, but the DBS samples often need to be transported from remote
sampling locations (e.g., tropical or developing countries) to analytical laboratories where the quantitative measurements
of different metabolic or disease biomarkers can be carried out. Dried blood spots are commonly method for collecting,
storing, transporting, and analysing a variety of human body fluids. Since 2006, however a variety of new and innovative
dried blood spots applications, although this pre-analytical approach represents an interesting alternative to classical
venous blood sampling, its routine use is limited. Here, we review the application of DBS technology in clinical
chemistry, molecular assays, small molecules, and large molecules all are perfectly suited to evaluate its future role
supported by new analytical methods such as mass spectrometry
Conference Presentations by Dr.Subhasree C.R.
preparation and processing of DBS for their final analysis has not been standardized. (1) compared to conventional
venepuncture, less blood volume is required and this fact was most important in paediatric diagnostics; (2) blood
collection is simple, non-invasive, and inexpensive; (3) the risk of bacterial contamination or haemolysis is minimal; and
(4) DBS can be preserved for long periods with almost no deterioration of the analytes. Advances techniques applied to
diagnose the disorders of Inborn errors of metabolism. Dried blood spots (DBSs) have potential use in remote health
applications for individual and population diagnosis, and can enable epidemiological surveillance for known and
unknown diseases. DBS sampling offers great potential for remote health applications as a cost-effective sample format
for large-scale screening and diagnostic purposes, but the DBS samples often need to be transported from remote
sampling locations (e.g., tropical or developing countries) to analytical laboratories where the quantitative measurements
of different metabolic or disease biomarkers can be carried out. Dried blood spots are commonly method for collecting,
storing, transporting, and analysing a variety of human body fluids. Since 2006, however a variety of new and innovative
dried blood spots applications, although this pre-analytical approach represents an interesting alternative to classical
venous blood sampling, its routine use is limited. Here, we review the application of DBS technology in clinical
chemistry, molecular assays, small molecules, and large molecules all are perfectly suited to evaluate its future role
supported by new analytical methods such as mass spectrometry