Papers by Mohammad Al Mahmud-Un-Nabi
Journal of advanced research, 2018
Patients with beta-thalassemia major (BTM) suffer from fatigue, poor physical fitness, muscle wea... more Patients with beta-thalassemia major (BTM) suffer from fatigue, poor physical fitness, muscle weakness, lethargy, and cardiac complications which are related to an energy crisis. Carnitine and acylcarnitine derivatives play important roles in fatty acid oxidation, and deregulation of carnitine and acylcarnitine metabolism may lead to an energy crisis. The present study aimed to investigate carnitine and acylcarnitine metabolites to gain an insight into the pathophysiology of BTM. Dried blood spots of 45 patients with BTM and 96 age-matched healthy controls were analyzed for free carnitine and 24 acylcarnitines by using liquid chromatography-tandem mass spectrometry (LC-MS/MS). Although medium chain acylcarnitine levels were similar in the patients with BTM and healthy controls, free carnitine, short chain acylcarnitines, long chain acylcarnitines, and total acylcarnitine levels were significantly lower in patients with BTM than in the healthy controls ( < 0.05). Moreover, an impa...
Characterisation of resistance phenotype and genotype is crucial to understanding the burden and ... more Characterisation of resistance phenotype and genotype is crucial to understanding the burden and transmission of antimicrobial resistance (AMR). This study aims to determine the spectrum of AMR and associated genes encoding aminoglycoside, macrolide and β‐lactam classes of antimicrobials in bacteria isolated from hospitalised patients in Bangladesh.
I hereby declare that the thesis project titled "Study of the Antimicrobial Activities of the Bar... more I hereby declare that the thesis project titled "Study of the Antimicrobial Activities of the Bark Extract of Cinnamomum verum on Multi Drug Resistant Klebsiella pneumoniae and Enterobacter agglomerans" submitted by me has been carried out under the supervision of Dr.
The study investigated the spectrum of antibiotic resistance and the associated genes for aminogl... more The study investigated the spectrum of antibiotic resistance and the associated genes for aminoglycoside, macrolide and ESBL class of antibiotics using clinical isolates. A total of 430 preserved bacterial strains (Acinetobacter baumannii, n= 20; Pseudomonas aeruginosa, n= 26; Klebsiella pneumoniae, n= 42; E.coli, n= 85; Staphylococcus aureus, n= 84; Salmonella Typhi, n= 82; Enterococcus spp., n= 27; Streptococcus pneumoniae, n= 36 and CNS, n= 28) were examined. The strains were isolated from patients admitted to various tertiary hospitals of Dhaka city between 2015 and 2019 with either acute respiratory infections, wound infections, typhoid fever or diarrhea. The isolates were reconfirmed by appropriate microbiological and biochemical methods. Antimicrobial susceptibility tests were done using Kirby-Bauer disk diffusion approach. PCR amplification using resistance gene-specific primers for aminoglycoside, macrolide and ESBL class of antibiotics was done and the amplified products were confirmed by Sanger sequencing. Of the total isolates, 53% came out as MDR with 96.6% of E. coli and 90% of Staphylococcus aureus. There was a year-wise gradual increase of MDR isolates from 2015-2018 and by 2019 the increase in MDR isolates became almost 2-fold compared to 2015. Among the five ESBL genes investigated, CTXM-1 came out as the most prevalent (63%) followed by NDM-1 (22%) and E. coli isolates were the predominant reservoir of these genes. ErmB (55%) was the most frequently detected macrolide resistance gene, whereas aac(6)-Ib (35.44%) was the most prevalent aminoglycoside resistance gene and these genes were most prevalent in E. coli and P. aeruginosa isolates, respectively. CTXM-1 and ErmB (16.66%) were the most frequent partners of coexistence followed by CTXM-1 and aac(3)-II. and is also made available for use under a CC0 license.
Journal of Advanced Research, 2018
Patients with beta-thalassemia major (BTM) suffer from fatigue, poor physical fitness, muscle wea... more Patients with beta-thalassemia major (BTM) suffer from fatigue, poor physical fitness, muscle weakness, lethargy, and cardiac complications which are related to an energy crisis. Carnitine and acylcarnitine derivatives play important roles in fatty acid oxidation, and deregulation of carnitine and acylcarnitine metabolism may lead to an energy crisis. The present study aimed to investigate carnitine and acylcarnitine metabolites to gain an insight into the pathophysiology of BTM. Dried blood spots of 45 patients with BTM and 96 age-matched healthy controls were analyzed for free carnitine and 24 acylcarnitines by using liquid chromatography-tandem mass spectrometry (LC-MS/MS). Although medium chain acylcarnitine levels were similar in the patients with BTM and healthy controls, free carnitine, short chain acylcarnitines, long chain acylcarnitines, and total acylcarnitine levels were significantly lower inpatients with BTM than in the healthy controls (P< 0.05). Moreover, an impaired fatty acid oxidation ratewas observed in the patients with BTM, as manifested by decreased fatty acid oxidation indicator ratios, namely C2/C0 and (C2 + C3)/C0. Furthermore, an increase in the C0/(C16 + C18) ratio indicated reduced carnitine palmitoyltransferase-1 (CPT-1) activity in the patients with BTM compared with that in the healthy controls. Thus, a low level of free carnitine and acylcarnitines together with impaired CPT-1 activity contribute to energy crisis-related complications in the patients with BTM.
Infections caused by Multi Drug Resistant (MDR) organisms cause to stand a worldwide pandemic. MD... more Infections caused by Multi Drug Resistant (MDR) organisms cause to stand a worldwide pandemic. MDR bacteria are an emerging threat with limited therapeutic options. Without having information about these strains, morbidity and mortality due to infections caused by these notorious bugs cannot be reduced. Alternative antimicrobial agent is needed beside the antibiotics to combat against these MDR organisms. Cinnamon is a spice obtained from the inner bark of trees of Cinnamomum sp. Traditionally it has also been used for medicinal purposes. In the current work we evaluated the antimicrobial activity of the cinnamon extract against MDR Klebsiella pneumoniae and Enterobacter aggalomerans, and also compared the results with drug sensitive organisms of the same species. Cinnamon extract was prepared with ethanol extraction procedure. The extract was dried and re-dissolved in Dimethyl sulfoxide (DMSO). The prepared extract was tested on the organisms by using agar diffusion technique followed by determination of Minimum Inhibitory Concentration (MIC) and Minimum Bactericidal Concentration (MBC) of the cinnamon extract. From the agar diffusion test the minimum concentration of cinnamon extracts needed to visualise the zone of inhibition on the Mueller Hinton Agar was determined to be 40 mg/ml. The MIC of the cinnamon extract was found to be 1 mg/ml and the MBC was determined as 2 mg/ml. The results obtained from this study can act as the stepping stone in investigating the antimicrobial properties of cinnamon extract. This study could be the beginning of discovering a new antimicrobial agent against the MDR organisms.
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Papers by Mohammad Al Mahmud-Un-Nabi