Human ESCs provide access to the earliest stages of human development and may serve as an unlimit... more Human ESCs provide access to the earliest stages of human development and may serve as an unlimited source of functional cells for future cell therapies. The optimization of methods directing the differentiation of human embryonic stem cells (hESCs) into tissue-specific precursors becomes crucial. We report an efficient enrichment of mesenchymal stem cells (MSCs) from hESCs through specific inhibition of SMAD-2/3 signaling. Human ESC-derived MSCs (hESC-MSCs) emerged as a population of fibroblastoid cells expressing a MSC phenotype: CD731 CD901 CD1051 CD441 CD1661 CD452 CD342 CD142 CD192 human leucocyte antigen-DR (HLA-DR)2. After 28 days of SMAD-2/3 inhibition, hESC cultures were enriched (>42%) in multipotent MSCs. CD731CD901 hESC-MSCs were fluorescence activated cell sorting (FACS)-isolated and longterm cultures were established and maintained for many passages displaying a faster growth than somatic tissuederived MSCs while maintaining MSC morphology and phenotype. They displayed osteogenic, adipogenic, and chondrocytic differentiation potential and exhibited potent immunosuppressive and anti-inflammatory properties in vitro and in vivo, where hESC-MSCs were capable of protecting against an experimental model of inflammatory bowel disease. Interestingly, the efficient enrichment of hESCs into MSCs through inhibition of SMAD-2/3 signaling was not reproducible with distinct induced pluripotent stem cell lines. Our findings provide mechanistic insights into the differentiation of hESCs into immunosuppressive and anti-inflammatory multipotent MSCs with potential future clinical applications.
Recebido 07 de junho de 2001; revisado 14 de setembro; aceito 22 de janeiro de 2002 RESUMO -Inúme... more Recebido 07 de junho de 2001; revisado 14 de setembro; aceito 22 de janeiro de 2002 RESUMO -Inúmeras metodologias vêm sendo desenvolvidas com o intuito de entender os fatores que condicionam o processo de movimento de massa. Neste propósito foi desenvolvido o modelo SHALSTAB que objetiva identificar e mapear as áreas de instabilidade nas encostas. Essa metodologia tem como referencial a modelagem matemática baseada na combinação de um modelo hidrológico com um modelo de estabilidade de encostas. Recentemente ela foi escrita em linguagem Avenue, sendo implementada para utilização no Software ArcView. Essa metodologia foi aplicada na região do Quadrilátero Ferrífero (MG) objetivando a identificação das áreas mais susceptíveis a ocorrência de escorregamentos rasos nessa região. Inicialmente, foi gerado um MDT e posteriormente foram determinados seus mapas derivados como V. M. Ramos et al. 50 área de contribuição e declividade. Os resultados obtidos demonstraram que, mesmo utilizando dados de uma escala relativamente pequena para esse propósito (1:50.000) o modelo SHALSTAB mostrou-se uma ferramenta eficaz para identificar zonas susceptíveis a escorregamentos rasos. Palavras Chave: encostas, escorregamentos rasos, SHALSTAB ABSTRACT -Many methodologies have been developed in order to understand the physical factors that control the mass movement process. In this purpose was developed the SHALSTAB model that identify instability areas in the hillslope. This methodology is based on the combination of a hydrological model with the slope stability model.
IGARSS 2003. 2003 IEEE International Geoscience and Remote Sensing Symposium. Proceedings (IEEE Cat. No.03CH37477), 2003
Landslides is a common problem in the southeast of Brazil mainly after strong summer rainfalls. T... more Landslides is a common problem in the southeast of Brazil mainly after strong summer rainfalls. The understanding of this process has been awaking the interest of the scholars. Many methodologies have been developed in order to identify areas where landslide prone. A methodology named SHALSTAB has been used and/or refined in order to mapping instability areas in the hillslope. This methodology is a combination between a hydrological model and a slope stability model, based on a digital elevation model (DEM). Recently it was written in Avenue language and implemented for utilization in the ArcView Software. The methodology was applied in the mine region named Quadrilátero Ferrífero (BRAZIL). A DEM was made and also were determined their derivatives maps as slope and contributing area. The soil properties parameters values were obtained from references. The results demonstrated that the SHALSTAB model is also an effective tool to the identification of susceptible zones for the occurrence of shallow landslides at mine zones.
Celestite-barite occurrences of early Cretaceous age w e r e studied in the Extra-A n d e a n reg... more Celestite-barite occurrences of early Cretaceous age w e r e studied in the Extra-A n d e a n region of Neuqu~n, W e s t central Argentina. The ores are located in a Subandean IV[esozoic belt and show a conspicuous stratabound character related to evaporitic facies. The study of the geologic evolution of the sedimentary sequence allows prediction of the temporal and spatial setting of the evaporitic facies. Stratigraphy is the main geologic control for the celestite-barite ores and their areal distribution depends on the structure of the region. Based on the geometry and texture of the mineralization three types of ores have been recognized: i) celestite beds syngenetic with the evaporitic facies; 2) epigenetic stratabound ores formed by "in situ" mobilization of celestite with stalactite growth and cavity infilling; 3) epigenetic vein-type ores formed by mobilized barium enriched solutions.
ln the Sierras Pampeanas geomorphic province of central Argentina there are regions where exposed... more ln the Sierras Pampeanas geomorphic province of central Argentina there are regions where exposed crystalline basement has had no sedimentary cover older than Neogene strata, and other areas where upper Paleozoic and Mesozoic strata overlie the basement. Both types of areas are characterized by a smooth upper basement surface, which has been interpreted as a peneplain that has been deformed and exhumed during the Neogene. The history of generation of the basement peneplain can be determined from thermochronometric and stratigraphic data. This reconnaissance study produced new data on the cooling history of rocks from seven sites ill the Sierras Pampeanas Fission-track cooling ages of apatite crystals, 40 9 .
Human ESCs provide access to the earliest stages of human development and may serve as an unlimit... more Human ESCs provide access to the earliest stages of human development and may serve as an unlimited source of functional cells for future cell therapies. The optimization of methods directing the differentiation of human embryonic stem cells (hESCs) into tissue-specific precursors becomes crucial. We report an efficient enrichment of mesenchymal stem cells (MSCs) from hESCs through specific inhibition of SMAD-2/3 signaling. Human ESC-derived MSCs (hESC-MSCs) emerged as a population of fibroblastoid cells expressing a MSC phenotype: CD731 CD901 CD1051 CD441 CD1661 CD452 CD342 CD142 CD192 human leucocyte antigen-DR (HLA-DR)2. After 28 days of SMAD-2/3 inhibition, hESC cultures were enriched (>42%) in multipotent MSCs. CD731CD901 hESC-MSCs were fluorescence activated cell sorting (FACS)-isolated and longterm cultures were established and maintained for many passages displaying a faster growth than somatic tissuederived MSCs while maintaining MSC morphology and phenotype. They displayed osteogenic, adipogenic, and chondrocytic differentiation potential and exhibited potent immunosuppressive and anti-inflammatory properties in vitro and in vivo, where hESC-MSCs were capable of protecting against an experimental model of inflammatory bowel disease. Interestingly, the efficient enrichment of hESCs into MSCs through inhibition of SMAD-2/3 signaling was not reproducible with distinct induced pluripotent stem cell lines. Our findings provide mechanistic insights into the differentiation of hESCs into immunosuppressive and anti-inflammatory multipotent MSCs with potential future clinical applications.
Recebido 07 de junho de 2001; revisado 14 de setembro; aceito 22 de janeiro de 2002 RESUMO -Inúme... more Recebido 07 de junho de 2001; revisado 14 de setembro; aceito 22 de janeiro de 2002 RESUMO -Inúmeras metodologias vêm sendo desenvolvidas com o intuito de entender os fatores que condicionam o processo de movimento de massa. Neste propósito foi desenvolvido o modelo SHALSTAB que objetiva identificar e mapear as áreas de instabilidade nas encostas. Essa metodologia tem como referencial a modelagem matemática baseada na combinação de um modelo hidrológico com um modelo de estabilidade de encostas. Recentemente ela foi escrita em linguagem Avenue, sendo implementada para utilização no Software ArcView. Essa metodologia foi aplicada na região do Quadrilátero Ferrífero (MG) objetivando a identificação das áreas mais susceptíveis a ocorrência de escorregamentos rasos nessa região. Inicialmente, foi gerado um MDT e posteriormente foram determinados seus mapas derivados como V. M. Ramos et al. 50 área de contribuição e declividade. Os resultados obtidos demonstraram que, mesmo utilizando dados de uma escala relativamente pequena para esse propósito (1:50.000) o modelo SHALSTAB mostrou-se uma ferramenta eficaz para identificar zonas susceptíveis a escorregamentos rasos. Palavras Chave: encostas, escorregamentos rasos, SHALSTAB ABSTRACT -Many methodologies have been developed in order to understand the physical factors that control the mass movement process. In this purpose was developed the SHALSTAB model that identify instability areas in the hillslope. This methodology is based on the combination of a hydrological model with the slope stability model.
IGARSS 2003. 2003 IEEE International Geoscience and Remote Sensing Symposium. Proceedings (IEEE Cat. No.03CH37477), 2003
Landslides is a common problem in the southeast of Brazil mainly after strong summer rainfalls. T... more Landslides is a common problem in the southeast of Brazil mainly after strong summer rainfalls. The understanding of this process has been awaking the interest of the scholars. Many methodologies have been developed in order to identify areas where landslide prone. A methodology named SHALSTAB has been used and/or refined in order to mapping instability areas in the hillslope. This methodology is a combination between a hydrological model and a slope stability model, based on a digital elevation model (DEM). Recently it was written in Avenue language and implemented for utilization in the ArcView Software. The methodology was applied in the mine region named Quadrilátero Ferrífero (BRAZIL). A DEM was made and also were determined their derivatives maps as slope and contributing area. The soil properties parameters values were obtained from references. The results demonstrated that the SHALSTAB model is also an effective tool to the identification of susceptible zones for the occurrence of shallow landslides at mine zones.
Celestite-barite occurrences of early Cretaceous age w e r e studied in the Extra-A n d e a n reg... more Celestite-barite occurrences of early Cretaceous age w e r e studied in the Extra-A n d e a n region of Neuqu~n, W e s t central Argentina. The ores are located in a Subandean IV[esozoic belt and show a conspicuous stratabound character related to evaporitic facies. The study of the geologic evolution of the sedimentary sequence allows prediction of the temporal and spatial setting of the evaporitic facies. Stratigraphy is the main geologic control for the celestite-barite ores and their areal distribution depends on the structure of the region. Based on the geometry and texture of the mineralization three types of ores have been recognized: i) celestite beds syngenetic with the evaporitic facies; 2) epigenetic stratabound ores formed by "in situ" mobilization of celestite with stalactite growth and cavity infilling; 3) epigenetic vein-type ores formed by mobilized barium enriched solutions.
ln the Sierras Pampeanas geomorphic province of central Argentina there are regions where exposed... more ln the Sierras Pampeanas geomorphic province of central Argentina there are regions where exposed crystalline basement has had no sedimentary cover older than Neogene strata, and other areas where upper Paleozoic and Mesozoic strata overlie the basement. Both types of areas are characterized by a smooth upper basement surface, which has been interpreted as a peneplain that has been deformed and exhumed during the Neogene. The history of generation of the basement peneplain can be determined from thermochronometric and stratigraphic data. This reconnaissance study produced new data on the cooling history of rocks from seven sites ill the Sierras Pampeanas Fission-track cooling ages of apatite crystals, 40 9 .
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