Background: Telomerase has been considered as an attractive molecular target for breast cancer th... more Background: Telomerase has been considered as an attractive molecular target for breast cancer therapy. The main objective of this work is to assess the inhibitory effects of silibinin and curcumin, two herbal substances, on telomerase gene expression in breast cancer cells. Materials and Methods: For determination of cell viability tetrazolium-based assays were conducted after 24, 48, and 72 h exposure times and expression of human telomerase reverse transcriptase gene was measured with real-time PCR. Results: Each compound exerted cytotoxic effects on T47D cells and inhibited telomerase gene expression, both in a time-and dose-dependent manner. The mixture of curcumin and silibinin showed relatively more inhibitory effect on growth of T47D cells and hTERT gene expression as compared with either agent alone. Conclusions: These findings suggest that cell viability along with hTERT gene expression in breast cancer cells could be reduced by curcumin and silibinin.
Background Obesity has been associated with increased mortality from hormone dependant cancers su... more Background Obesity has been associated with increased mortality from hormone dependant cancers such as breast cancer which is the most prevalent cancer in women. The link between obesity and breast cancer can be attributed to excess estrogen produced through aromatization in adipose tissue. The role of steroid hormone receptors in breast cancer development is well studied but how obesity can affect the expression pattern of steroid hormones in patients with different grades of breast cancer was the aim of this study. Methods In this case-control study, 70 women with breast cancer participated with different grades of obesity (36 none obese, BMI < 25 kg/m2 and 34 obese, BMI ≥ 25 kg/m2). The mean age of participants was 44.53 ± 1.79 yr (21–70 yr). The serum level of estrogen, progesterone and androgen determined by ELISA. Following quantitative expression of steroid hormone receptors mRNA in tumor tissues evaluated by Real-time PCR. Patients with previous history of radiotherapy or...
Leptin plays the role of mitogenic factor in the breast carcinogenesis. Therefore, it could be co... more Leptin plays the role of mitogenic factor in the breast carcinogenesis. Therefore, it could be considered as a target for breast cancer therapy. Leptin gene expression could be modulated by activation of estrogen receptors. Silibinin is an herbal compound with anti-cancer activity on prostate and colorectal cancers. Based on the fact that targeting of leptin can be considered as a novel strategy for breast cancer therapy, the aim of this study was the investigation of potentiality of silibinin for inhibition of leptin gene expression and secretion, and its link with expression of estrogen receptors. Cytotoxic effect of silibinin on T47D breast cancer cells was investigated by MTT assay test after 24, 48 and 72 h treatments with different concentrations of silibinin. The levels of leptin, estrogen receptor a and estrogen receptor b genes expression was measured by reverse-transcription real-time PCR. The amount of secreted leptin in the culture medium was determined by ELISA. Data were statistically analyzed by one-way ANOVA test. Silibinin inhibits growth of T47D cells in a time and dose dependent manner. There was significant difference between control and treated cells in the levels of leptin, estrogen receptor b expression levels and the quantity of secreted leptin was decreased in the treated cells in comparison to control cells. In conclusion, silibinin inhibits the expression and the secretion of leptin and in the future it might probably be a drug candidate for breast cancer therapy through leptin targeting.
The transient global cerebral ischemia was induced using four-vesselocclusion method for 20 min. ... more The transient global cerebral ischemia was induced using four-vesselocclusion method for 20 min. Lipid peroxidation level in hippocampus portion was measured as malondialdehyde (MDA) based on its reaction with thiobarbituric acid (TBA) following ischemic insult. Results: The transient global cerebral ischemia induced a significant increase in TBA reactive substances (TBARS) level (p < 0.001), in comparison to sham-operated animal. Pretreatment with TQ and NSO resulted to a significant decrease in MDA level as compared to ischemic group (66.9±1.5 vs. 297±2.5 nmol/g tissue for TQ, 10 mg/kg; p<0.001 and 153.5±1.3 nmol/g tissue for NSO, 0.384 mg/kg; p< 0.001). Using a reversed-phase HPLC system, the amount of TQ in NSO was 0.58% w/w. Conclusion: These results suggested that TQ and NSO may have protective effects on lipid peroxidation process during IRI in rat hippocampus.
High yield production of recombinant human Fibroblast Growth Factor-1 (rhFGF-1) in SHuffle™ T7 ... more High yield production of recombinant human Fibroblast Growth Factor-1 (rhFGF-1) in SHuffle™ T7 strain Production of 1500 mg of properly-folded, bioactive rhFGF-1 per liter of culture media Recruiting SHuffle™ T7 strain for high yield production of a nondisulfide bonded protein (rhFGF-1) Background: Human fibroblast growth factor-1 (FGF-1) has powerful mitogenic activities in a variety of cell types and plays significant roles in many physiological processes e.g. angiogenesis and wound healing. There is increasing demand for large scale production of recombinant human FGF-1 (rhFGF-1), in order to investigate the potential medical use. In the present study, we explored SHuffle™ T7 strain for production of rhFGF-1. Methods: A synthetic gene encoding Met-140 amino acid form of human FGF-1 was utilized for expression of the protein in three different E. coli hosts (BL21 (DE3), Rosetta-gami™ 2(DE3), SHuffle™ T7). Total expressions and soluble/insoluble expression ratios of rhFGF-1 in different hosts were analyzed and compared. Soluble rhFGF-1 produced in SHuffle™ T7 cells was purified using one-step heparin-Sepharose affinity chromatography and characterized by a variety of methods for physicochemical and biological properties. Results: The highest level of rhFGF-1 expression and maximum soluble/insoluble ratio were achieved in SHuffle™ T7 strain. Using a single-step heparin-Sepharose chromatography, about 1500 mg of purified rhFGF-1 was obtained from one liter of the culture, representing purification yield of ~70%. The purified protein was reactive toward anti-FGF-1 ployclonal antibody in immunoblotting. Mass spectrometry confirmed the protein had expected amino acid sequence and molecular weight. In reverse-phase highperformance liquid chromatography (RP-HPLC), the protein displayed the same retention time with the human FGF-1 standard, and purity of 94%. Less than 0.3% of the purified protein was comprised of oligomers and/or aggregates as judged by high-performance size-exclusion chromatography (HP-SEC). Secondary and tertiary structures of the protein, investigated by circular dichroism and intrinsic fluorescence spectroscopy methods, respectively, represented native folding of the protein. The purified rhFGF-1 was bioactive and stimulated proliferation of NIH 3T3 cells with EC50 of 0.84 ng/mL. Conclusion: Although SHuffle™ T7 has been introduced for production of disulfide-bonded proteins in cytoplasm, we herein successfully recruited it for high yield production of soluble and bioactive rhFGF-1, a protein with 3 free cysteine and no disulfide bond. To our knowledge, this is the highest-level of rhFGF-1 expression in E. coli reported so far. Extensive physicochemical and biological analysis showed the protein had similar characteristic to authentic FGF-1.
The transient global cerebral ischemia was induced using four-vesselocclusion method for 20 min. ... more The transient global cerebral ischemia was induced using four-vesselocclusion method for 20 min. Lipid peroxidation level in hippocampus portion was measured as malondialdehyde (MDA) based on its reaction with thiobarbituric acid (TBA) following ischemic insult. Results: The transient global cerebral ischemia induced a significant increase in TBA reactive substances (TBARS) level (p < 0.001), in comparison to sham-operated animal. Pretreatment with TQ and NSO resulted to a significant decrease in MDA level as compared to ischemic group (66.9±1.5 vs. 297±2.5 nmol/g tissue for TQ,10 mg/kg; p<0.001 and 153.5 ± 1.3 nmol/g tissue for NSO, 0.384 mg/kg; p < 0.001). Using a reversed-phase HPLC system, the amount of TQ in NSO was 0.58% w/w. Conclusion: These results suggested that TQ and NSO may have protective effects on lipid peroxidation process during IRI in rat hippocampus.
Background: Human fibroblast growth factor-1 (FGF-1) has powerful mitogenic activities in a varie... more Background: Human fibroblast growth factor-1 (FGF-1) has powerful mitogenic activities in a variety of cell types and plays significant roles in many physiological processes e.g. angiogenesis and wound healing. There is increasing demand for large scale production of recombinant human FGF-1 (rhFGF-1), in order to investigate the potential medical use. In the present study, we explored SHuffle™ T7 strain for production of rhFGF-1. Methods: A synthetic gene encoding Met-140 amino acid form of human FGF-1 was utilized for expression of the protein in three different E. coli hosts (BL21 (DE3), Rosetta-gami™ 2(DE3), SHuffle™ T7). Total expressions and soluble/insoluble expression ratios of rhFGF-1 in different hosts were analyzed and compared. Soluble rhFGF-1 produced in SHuffle™ T7 cells was purified using one-step heparin-Sepharose affinity chromatography and characterized by a variety of methods for physicochemical and biological properties. Results: The highest level of rhFGF-1 expression and maximum soluble/insoluble ratio were achieved in SHuffle™ T7 strain. Using a single-step heparin-Sepharose chromatography, about 1500 mg of purified rhFGF-1 was obtained from one liter of the culture, representing purification yield of ∼70%. The purified protein was reactive toward anti-FGF-1 ployclonal antibody in immunoblotting. Mass spectrometry confirmed the protein had expected amino acid sequence and molecular weight. In reverse-phase high-performance liquid chromatography (RP-HPLC), the protein displayed the same retention time with the human FGF-1 standard, and purity of 94%. Less than 0.3% of the purified protein was comprised of oligomers and/or aggregates as judged by high-performance size-exclusion chromatography (HP-SEC). Secondary and tertiary structures of the protein, investigated by circular dichroism and intrinsic fluorescence spectroscopy methods, respectively, represented native folding of the protein. The purified rhFGF-1 was bioactive and stimulated proliferation of NIH 3T3 cells with EC50 of 0.84 ng/mL. Conclusion: Although SHuffle™ T7 has been introduced for production of disulfide-bonded proteins in cytoplasm, we herein successfully recruited it for high yield production of soluble and bioactive rhFGF-1, a protein with 3 free cysteine and no disulfide bond. To our knowledge, this is the highest-level of rhFGF-1 expression in E. coli reported so far. Extensive physicochemical and biological analysis showed the protein had similar characteristic to authentic FGF-1.
Telomerase is a ribonucleoprotein enzyme, which has a
significant role in synthesizing DNA telome... more Telomerase is a ribonucleoprotein enzyme, which has a significant role in synthesizing DNA telomeric in eukaryotes. Telomere maintenance can cause to immortalization and malignant transformation of human cells and thereby telomerase activity must be scrutinized as an important factor in most tumor cells. The proliferation of cancer cells or apoptosis induction can be suppressed by telomerase inhibition using different therapeutic agents without any side effects upon normal cells. Natural substances, with anti-tumor effects, such as those derived from plants can be suitable candidates due to their capabilities in preventing some side effects and resistance of tumors with respect to most chemotherapeutic drugs. In this regards, many studies have shown that natural phytochemicals have inhibitory effects on telomerase activity through affecting its subunits and components. Therefore, the aim of this paper is to review the recent studies on these kinds of phytochemicals in terms of property and mechanism. Moreover, strategies for improving the therapeutic efficacy of plant-derived substances such as combination therapy and nanoformulation based approaches are included.
Background: Obesity has been associated with increased mortality from hormone dependant cancers s... more Background: Obesity has been associated with increased mortality from hormone dependant cancers such as breast cancer which is the most prevalent cancer in women. The link between obesity and breast cancer can be attributed to excess estrogen produced through aromatization in adipose tissue. The role of steroid hormone receptors in breast cancer development is well studied but how obesity can affect the expression pattern of steroid hormones in patients with different grades of breast cancer was the aim of this study. Methods: In this case-control study, 70 women with breast cancer participated with different grades of obesity (36 none obese, BMI , 25 kg/m 2 and 34 obese, BMI $ 25 kg/m 2). The mean age of participants was 44.53 ± 1.79 yr (21-70 yr). The serum level of estrogen, progesterone and androgen determined by ELISA. Following quantitative expression of steroid hormone receptors mRNA in tumor tissues evaluated by Real-time PCR. Patients with previous history of radiotherapy or chemotherapy were excluded. SPSS 16 was used for data analysis and P , 0.05 considered statistically significant. Results: The difference in ERα, ERβ and PR mRNA level between normal and obese patients was significant (P , 0.001). In addition, the expression of AR mRNA was found to be higher than other steroid receptors. There was no significant relation between ERβ gene expression in two groups (P = 0.68). We observed a significant relationship between ERα and AR mRNA with tumor stage and tumor grade, respectively (P = 0.023, P = 0.015). Conclusion: According to the obtained results, it is speculated that obesity could paly a significant role in estrogen receptors gene expression and also could affect progression and proliferation of breast cancer cells.
Leptin plays the role of mitogenic factor in the breast carcinogenesis. Therefore, it could be co... more Leptin plays the role of mitogenic factor in the breast carcinogenesis. Therefore, it could be considered as a target for breast cancer therapy. Leptin gene expression could be modulated by activation of estrogen receptors. Silibinin is an herbal compound with anti-cancer activity on prostate and colorectal cancers. Based on the fact that targeting of leptin can be considered as a novel strategy for breast cancer therapy, the aim of this study was the investigation of potentiality of silibinin for inhibition of leptin gene expression and secretion, and its link with expression of estrogen receptors. Cytotoxic effect of silibinin on T47D breast cancer cells was investigated by MTT assay test after 24, 48 and 72 h treatments with different concentrations of silibinin. The levels of leptin, estrogen receptor a and estrogen receptor b genes expression was measured by reverse-transcription real-time PCR. The amount of secreted leptin in the culture medium was determined by ELISA. Data were statistically analyzed by one-way ANOVA test. Silibinin inhibits growth of T47D cells in a time and dose dependent manner. There was significant difference between control and treated cells in the levels of leptin, estrogen receptor b expression levels and the quantity of secreted leptin was decreased in the treated cells in comparison to control cells. In conclusion, silibinin inhibits the expression and the secretion of leptin and in the future it might probably be a drug candidate for breast cancer therapy through leptin targeting.
Background: Telomerase has been considered as an attractive molecular target for breast cancer th... more Background: Telomerase has been considered as an attractive molecular target for breast cancer therapy. The main objective of this work is to assess the inhibitory effects of silibinin and curcumin, two herbal substances, on telomerase gene expression in breast cancer cells. Materials and Methods: For determination of cell viability tetrazolium-based assays were conducted after 24, 48, and 72 h exposure times and expression of human telomerase reverse transcriptase gene was measured with real-time PCR. Results: Each compound exerted cytotoxic effects on T47D cells and inhibited telomerase gene expression, both in a time-and dose-dependent manner. The mixture of curcumin and silibinin showed relatively more inhibitory effect on growth of T47D cells and hTERT gene expression as compared with either agent alone. Conclusions: These findings suggest that cell viability along with hTERT gene expression in breast cancer cells could be reduced by curcumin and silibinin.
Background: Telomerase has been considered as an attractive molecular target for breast cancer th... more Background: Telomerase has been considered as an attractive molecular target for breast cancer therapy. The main objective of this work is to assess the inhibitory effects of silibinin and curcumin, two herbal substances, on telomerase gene expression in breast cancer cells. Materials and Methods: For determination of cell viability tetrazolium-based assays were conducted after 24, 48, and 72 h exposure times and expression of human telomerase reverse transcriptase gene was measured with real-time PCR. Results: Each compound exerted cytotoxic effects on T47D cells and inhibited telomerase gene expression, both in a time-and dose-dependent manner. The mixture of curcumin and silibinin showed relatively more inhibitory effect on growth of T47D cells and hTERT gene expression as compared with either agent alone. Conclusions: These findings suggest that cell viability along with hTERT gene expression in breast cancer cells could be reduced by curcumin and silibinin.
reticulum of eukaryotes, in the chloroplasts of plants, in photosynthetic microorganisms, and in ... more reticulum of eukaryotes, in the chloroplasts of plants, in photosynthetic microorganisms, and in bacteria. Fullerene is any molecule composed entirely of carbon, in the form of a hollow sphere, ellipsoid, or tube. Fullerenes were under study for potential medicinal uses. They were utilized as the carriers of genes and drugs. Method: The Planck's theorem emphasized that the energy was transferred in chunks known as quanta, equal to the energy of the photon of electromagnetic region. The Planck's equation allows calculating the frequency of photons, given their energy. If the frequency is given, the wavelength can be determined by using the wave equation. In this study, the number of carbon atoms in the fullerenes was used as an index to establish a relationship between the structure of cytochromes c, b, a3, p-450 as the most well-know redox systems and fullerenes Cn(n = 60, 70, 76, 82 and 86), which create [cytochrome c]. Cn, A-1 to A-5, [cytochrome b]. Cn, B-1 to B-5, [cytochrome a3]. Cn, C1-C5, [cytochrome p-450]. Cn, D1-D5. The relationship between the number of carbon atoms and the free energies of electron transfer is assessed using the ET equation for A-1 to A-5, B-1 to B-5, C-1 to C-5 and D-1 to D-5 nanosupramolecular [Y]. Cn (Y = cytochrome c, cytochrome b, cytochrome a3, Cytochrome p-450) complexes. Results and conclusion: The calculations were presented for the oxidation potentials of fullerenes Cn. The results were used to calculate maximum wavelength (λmax) and the rate constants (ket) of the photo electron transfer process in the nanosupramolecular complexes A-1 to A-5, B-1 to B-5, C-1 to C-5 and D-1 to D-5 for fullerenes Cn (n= 60-300).
Background: Telomerase has been considered as an attractive molecular target for breast cancer th... more Background: Telomerase has been considered as an attractive molecular target for breast cancer therapy. The main objective of this work is to assess the inhibitory effects of silibinin and curcumin, two herbal substances, on telomerase gene expression in breast cancer cells. Materials and Methods: For determination of cell viability tetrazolium-based assays were conducted after 24, 48, and 72 h exposure times and expression of human telomerase reverse transcriptase gene was measured with real-time PCR. Results: Each compound exerted cytotoxic effects on T47D cells and inhibited telomerase gene expression, both in a time-and dose-dependent manner. The mixture of curcumin and silibinin showed relatively more inhibitory effect on growth of T47D cells and hTERT gene expression as compared with either agent alone. Conclusions: These findings suggest that cell viability along with hTERT gene expression in breast cancer cells could be reduced by curcumin and silibinin.
Background Obesity has been associated with increased mortality from hormone dependant cancers su... more Background Obesity has been associated with increased mortality from hormone dependant cancers such as breast cancer which is the most prevalent cancer in women. The link between obesity and breast cancer can be attributed to excess estrogen produced through aromatization in adipose tissue. The role of steroid hormone receptors in breast cancer development is well studied but how obesity can affect the expression pattern of steroid hormones in patients with different grades of breast cancer was the aim of this study. Methods In this case-control study, 70 women with breast cancer participated with different grades of obesity (36 none obese, BMI < 25 kg/m2 and 34 obese, BMI ≥ 25 kg/m2). The mean age of participants was 44.53 ± 1.79 yr (21–70 yr). The serum level of estrogen, progesterone and androgen determined by ELISA. Following quantitative expression of steroid hormone receptors mRNA in tumor tissues evaluated by Real-time PCR. Patients with previous history of radiotherapy or...
Leptin plays the role of mitogenic factor in the breast carcinogenesis. Therefore, it could be co... more Leptin plays the role of mitogenic factor in the breast carcinogenesis. Therefore, it could be considered as a target for breast cancer therapy. Leptin gene expression could be modulated by activation of estrogen receptors. Silibinin is an herbal compound with anti-cancer activity on prostate and colorectal cancers. Based on the fact that targeting of leptin can be considered as a novel strategy for breast cancer therapy, the aim of this study was the investigation of potentiality of silibinin for inhibition of leptin gene expression and secretion, and its link with expression of estrogen receptors. Cytotoxic effect of silibinin on T47D breast cancer cells was investigated by MTT assay test after 24, 48 and 72 h treatments with different concentrations of silibinin. The levels of leptin, estrogen receptor a and estrogen receptor b genes expression was measured by reverse-transcription real-time PCR. The amount of secreted leptin in the culture medium was determined by ELISA. Data were statistically analyzed by one-way ANOVA test. Silibinin inhibits growth of T47D cells in a time and dose dependent manner. There was significant difference between control and treated cells in the levels of leptin, estrogen receptor b expression levels and the quantity of secreted leptin was decreased in the treated cells in comparison to control cells. In conclusion, silibinin inhibits the expression and the secretion of leptin and in the future it might probably be a drug candidate for breast cancer therapy through leptin targeting.
The transient global cerebral ischemia was induced using four-vesselocclusion method for 20 min. ... more The transient global cerebral ischemia was induced using four-vesselocclusion method for 20 min. Lipid peroxidation level in hippocampus portion was measured as malondialdehyde (MDA) based on its reaction with thiobarbituric acid (TBA) following ischemic insult. Results: The transient global cerebral ischemia induced a significant increase in TBA reactive substances (TBARS) level (p < 0.001), in comparison to sham-operated animal. Pretreatment with TQ and NSO resulted to a significant decrease in MDA level as compared to ischemic group (66.9±1.5 vs. 297±2.5 nmol/g tissue for TQ, 10 mg/kg; p<0.001 and 153.5±1.3 nmol/g tissue for NSO, 0.384 mg/kg; p< 0.001). Using a reversed-phase HPLC system, the amount of TQ in NSO was 0.58% w/w. Conclusion: These results suggested that TQ and NSO may have protective effects on lipid peroxidation process during IRI in rat hippocampus.
High yield production of recombinant human Fibroblast Growth Factor-1 (rhFGF-1) in SHuffle™ T7 ... more High yield production of recombinant human Fibroblast Growth Factor-1 (rhFGF-1) in SHuffle™ T7 strain Production of 1500 mg of properly-folded, bioactive rhFGF-1 per liter of culture media Recruiting SHuffle™ T7 strain for high yield production of a nondisulfide bonded protein (rhFGF-1) Background: Human fibroblast growth factor-1 (FGF-1) has powerful mitogenic activities in a variety of cell types and plays significant roles in many physiological processes e.g. angiogenesis and wound healing. There is increasing demand for large scale production of recombinant human FGF-1 (rhFGF-1), in order to investigate the potential medical use. In the present study, we explored SHuffle™ T7 strain for production of rhFGF-1. Methods: A synthetic gene encoding Met-140 amino acid form of human FGF-1 was utilized for expression of the protein in three different E. coli hosts (BL21 (DE3), Rosetta-gami™ 2(DE3), SHuffle™ T7). Total expressions and soluble/insoluble expression ratios of rhFGF-1 in different hosts were analyzed and compared. Soluble rhFGF-1 produced in SHuffle™ T7 cells was purified using one-step heparin-Sepharose affinity chromatography and characterized by a variety of methods for physicochemical and biological properties. Results: The highest level of rhFGF-1 expression and maximum soluble/insoluble ratio were achieved in SHuffle™ T7 strain. Using a single-step heparin-Sepharose chromatography, about 1500 mg of purified rhFGF-1 was obtained from one liter of the culture, representing purification yield of ~70%. The purified protein was reactive toward anti-FGF-1 ployclonal antibody in immunoblotting. Mass spectrometry confirmed the protein had expected amino acid sequence and molecular weight. In reverse-phase highperformance liquid chromatography (RP-HPLC), the protein displayed the same retention time with the human FGF-1 standard, and purity of 94%. Less than 0.3% of the purified protein was comprised of oligomers and/or aggregates as judged by high-performance size-exclusion chromatography (HP-SEC). Secondary and tertiary structures of the protein, investigated by circular dichroism and intrinsic fluorescence spectroscopy methods, respectively, represented native folding of the protein. The purified rhFGF-1 was bioactive and stimulated proliferation of NIH 3T3 cells with EC50 of 0.84 ng/mL. Conclusion: Although SHuffle™ T7 has been introduced for production of disulfide-bonded proteins in cytoplasm, we herein successfully recruited it for high yield production of soluble and bioactive rhFGF-1, a protein with 3 free cysteine and no disulfide bond. To our knowledge, this is the highest-level of rhFGF-1 expression in E. coli reported so far. Extensive physicochemical and biological analysis showed the protein had similar characteristic to authentic FGF-1.
The transient global cerebral ischemia was induced using four-vesselocclusion method for 20 min. ... more The transient global cerebral ischemia was induced using four-vesselocclusion method for 20 min. Lipid peroxidation level in hippocampus portion was measured as malondialdehyde (MDA) based on its reaction with thiobarbituric acid (TBA) following ischemic insult. Results: The transient global cerebral ischemia induced a significant increase in TBA reactive substances (TBARS) level (p < 0.001), in comparison to sham-operated animal. Pretreatment with TQ and NSO resulted to a significant decrease in MDA level as compared to ischemic group (66.9±1.5 vs. 297±2.5 nmol/g tissue for TQ,10 mg/kg; p<0.001 and 153.5 ± 1.3 nmol/g tissue for NSO, 0.384 mg/kg; p < 0.001). Using a reversed-phase HPLC system, the amount of TQ in NSO was 0.58% w/w. Conclusion: These results suggested that TQ and NSO may have protective effects on lipid peroxidation process during IRI in rat hippocampus.
Background: Human fibroblast growth factor-1 (FGF-1) has powerful mitogenic activities in a varie... more Background: Human fibroblast growth factor-1 (FGF-1) has powerful mitogenic activities in a variety of cell types and plays significant roles in many physiological processes e.g. angiogenesis and wound healing. There is increasing demand for large scale production of recombinant human FGF-1 (rhFGF-1), in order to investigate the potential medical use. In the present study, we explored SHuffle™ T7 strain for production of rhFGF-1. Methods: A synthetic gene encoding Met-140 amino acid form of human FGF-1 was utilized for expression of the protein in three different E. coli hosts (BL21 (DE3), Rosetta-gami™ 2(DE3), SHuffle™ T7). Total expressions and soluble/insoluble expression ratios of rhFGF-1 in different hosts were analyzed and compared. Soluble rhFGF-1 produced in SHuffle™ T7 cells was purified using one-step heparin-Sepharose affinity chromatography and characterized by a variety of methods for physicochemical and biological properties. Results: The highest level of rhFGF-1 expression and maximum soluble/insoluble ratio were achieved in SHuffle™ T7 strain. Using a single-step heparin-Sepharose chromatography, about 1500 mg of purified rhFGF-1 was obtained from one liter of the culture, representing purification yield of ∼70%. The purified protein was reactive toward anti-FGF-1 ployclonal antibody in immunoblotting. Mass spectrometry confirmed the protein had expected amino acid sequence and molecular weight. In reverse-phase high-performance liquid chromatography (RP-HPLC), the protein displayed the same retention time with the human FGF-1 standard, and purity of 94%. Less than 0.3% of the purified protein was comprised of oligomers and/or aggregates as judged by high-performance size-exclusion chromatography (HP-SEC). Secondary and tertiary structures of the protein, investigated by circular dichroism and intrinsic fluorescence spectroscopy methods, respectively, represented native folding of the protein. The purified rhFGF-1 was bioactive and stimulated proliferation of NIH 3T3 cells with EC50 of 0.84 ng/mL. Conclusion: Although SHuffle™ T7 has been introduced for production of disulfide-bonded proteins in cytoplasm, we herein successfully recruited it for high yield production of soluble and bioactive rhFGF-1, a protein with 3 free cysteine and no disulfide bond. To our knowledge, this is the highest-level of rhFGF-1 expression in E. coli reported so far. Extensive physicochemical and biological analysis showed the protein had similar characteristic to authentic FGF-1.
Telomerase is a ribonucleoprotein enzyme, which has a
significant role in synthesizing DNA telome... more Telomerase is a ribonucleoprotein enzyme, which has a significant role in synthesizing DNA telomeric in eukaryotes. Telomere maintenance can cause to immortalization and malignant transformation of human cells and thereby telomerase activity must be scrutinized as an important factor in most tumor cells. The proliferation of cancer cells or apoptosis induction can be suppressed by telomerase inhibition using different therapeutic agents without any side effects upon normal cells. Natural substances, with anti-tumor effects, such as those derived from plants can be suitable candidates due to their capabilities in preventing some side effects and resistance of tumors with respect to most chemotherapeutic drugs. In this regards, many studies have shown that natural phytochemicals have inhibitory effects on telomerase activity through affecting its subunits and components. Therefore, the aim of this paper is to review the recent studies on these kinds of phytochemicals in terms of property and mechanism. Moreover, strategies for improving the therapeutic efficacy of plant-derived substances such as combination therapy and nanoformulation based approaches are included.
Background: Obesity has been associated with increased mortality from hormone dependant cancers s... more Background: Obesity has been associated with increased mortality from hormone dependant cancers such as breast cancer which is the most prevalent cancer in women. The link between obesity and breast cancer can be attributed to excess estrogen produced through aromatization in adipose tissue. The role of steroid hormone receptors in breast cancer development is well studied but how obesity can affect the expression pattern of steroid hormones in patients with different grades of breast cancer was the aim of this study. Methods: In this case-control study, 70 women with breast cancer participated with different grades of obesity (36 none obese, BMI , 25 kg/m 2 and 34 obese, BMI $ 25 kg/m 2). The mean age of participants was 44.53 ± 1.79 yr (21-70 yr). The serum level of estrogen, progesterone and androgen determined by ELISA. Following quantitative expression of steroid hormone receptors mRNA in tumor tissues evaluated by Real-time PCR. Patients with previous history of radiotherapy or chemotherapy were excluded. SPSS 16 was used for data analysis and P , 0.05 considered statistically significant. Results: The difference in ERα, ERβ and PR mRNA level between normal and obese patients was significant (P , 0.001). In addition, the expression of AR mRNA was found to be higher than other steroid receptors. There was no significant relation between ERβ gene expression in two groups (P = 0.68). We observed a significant relationship between ERα and AR mRNA with tumor stage and tumor grade, respectively (P = 0.023, P = 0.015). Conclusion: According to the obtained results, it is speculated that obesity could paly a significant role in estrogen receptors gene expression and also could affect progression and proliferation of breast cancer cells.
Leptin plays the role of mitogenic factor in the breast carcinogenesis. Therefore, it could be co... more Leptin plays the role of mitogenic factor in the breast carcinogenesis. Therefore, it could be considered as a target for breast cancer therapy. Leptin gene expression could be modulated by activation of estrogen receptors. Silibinin is an herbal compound with anti-cancer activity on prostate and colorectal cancers. Based on the fact that targeting of leptin can be considered as a novel strategy for breast cancer therapy, the aim of this study was the investigation of potentiality of silibinin for inhibition of leptin gene expression and secretion, and its link with expression of estrogen receptors. Cytotoxic effect of silibinin on T47D breast cancer cells was investigated by MTT assay test after 24, 48 and 72 h treatments with different concentrations of silibinin. The levels of leptin, estrogen receptor a and estrogen receptor b genes expression was measured by reverse-transcription real-time PCR. The amount of secreted leptin in the culture medium was determined by ELISA. Data were statistically analyzed by one-way ANOVA test. Silibinin inhibits growth of T47D cells in a time and dose dependent manner. There was significant difference between control and treated cells in the levels of leptin, estrogen receptor b expression levels and the quantity of secreted leptin was decreased in the treated cells in comparison to control cells. In conclusion, silibinin inhibits the expression and the secretion of leptin and in the future it might probably be a drug candidate for breast cancer therapy through leptin targeting.
Background: Telomerase has been considered as an attractive molecular target for breast cancer th... more Background: Telomerase has been considered as an attractive molecular target for breast cancer therapy. The main objective of this work is to assess the inhibitory effects of silibinin and curcumin, two herbal substances, on telomerase gene expression in breast cancer cells. Materials and Methods: For determination of cell viability tetrazolium-based assays were conducted after 24, 48, and 72 h exposure times and expression of human telomerase reverse transcriptase gene was measured with real-time PCR. Results: Each compound exerted cytotoxic effects on T47D cells and inhibited telomerase gene expression, both in a time-and dose-dependent manner. The mixture of curcumin and silibinin showed relatively more inhibitory effect on growth of T47D cells and hTERT gene expression as compared with either agent alone. Conclusions: These findings suggest that cell viability along with hTERT gene expression in breast cancer cells could be reduced by curcumin and silibinin.
Background: Telomerase has been considered as an attractive molecular target for breast cancer th... more Background: Telomerase has been considered as an attractive molecular target for breast cancer therapy. The main objective of this work is to assess the inhibitory effects of silibinin and curcumin, two herbal substances, on telomerase gene expression in breast cancer cells. Materials and Methods: For determination of cell viability tetrazolium-based assays were conducted after 24, 48, and 72 h exposure times and expression of human telomerase reverse transcriptase gene was measured with real-time PCR. Results: Each compound exerted cytotoxic effects on T47D cells and inhibited telomerase gene expression, both in a time-and dose-dependent manner. The mixture of curcumin and silibinin showed relatively more inhibitory effect on growth of T47D cells and hTERT gene expression as compared with either agent alone. Conclusions: These findings suggest that cell viability along with hTERT gene expression in breast cancer cells could be reduced by curcumin and silibinin.
reticulum of eukaryotes, in the chloroplasts of plants, in photosynthetic microorganisms, and in ... more reticulum of eukaryotes, in the chloroplasts of plants, in photosynthetic microorganisms, and in bacteria. Fullerene is any molecule composed entirely of carbon, in the form of a hollow sphere, ellipsoid, or tube. Fullerenes were under study for potential medicinal uses. They were utilized as the carriers of genes and drugs. Method: The Planck's theorem emphasized that the energy was transferred in chunks known as quanta, equal to the energy of the photon of electromagnetic region. The Planck's equation allows calculating the frequency of photons, given their energy. If the frequency is given, the wavelength can be determined by using the wave equation. In this study, the number of carbon atoms in the fullerenes was used as an index to establish a relationship between the structure of cytochromes c, b, a3, p-450 as the most well-know redox systems and fullerenes Cn(n = 60, 70, 76, 82 and 86), which create [cytochrome c]. Cn, A-1 to A-5, [cytochrome b]. Cn, B-1 to B-5, [cytochrome a3]. Cn, C1-C5, [cytochrome p-450]. Cn, D1-D5. The relationship between the number of carbon atoms and the free energies of electron transfer is assessed using the ET equation for A-1 to A-5, B-1 to B-5, C-1 to C-5 and D-1 to D-5 nanosupramolecular [Y]. Cn (Y = cytochrome c, cytochrome b, cytochrome a3, Cytochrome p-450) complexes. Results and conclusion: The calculations were presented for the oxidation potentials of fullerenes Cn. The results were used to calculate maximum wavelength (λmax) and the rate constants (ket) of the photo electron transfer process in the nanosupramolecular complexes A-1 to A-5, B-1 to B-5, C-1 to C-5 and D-1 to D-5 for fullerenes Cn (n= 60-300).
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Papers by marzie nasiri
significant role in synthesizing DNA telomeric in eukaryotes. Telomere
maintenance can cause to immortalization and malignant transformation of
human cells and thereby telomerase activity must be scrutinized as an
important factor in most tumor cells. The proliferation of cancer cells or
apoptosis induction can be suppressed by telomerase inhibition using
different therapeutic agents without any side effects upon normal cells.
Natural substances, with anti-tumor effects, such as those derived from
plants can be suitable candidates due to their capabilities in preventing
some side effects and resistance of tumors with respect to most
chemotherapeutic drugs. In this regards, many studies have shown that
natural phytochemicals have inhibitory effects on telomerase activity
through affecting its subunits and components. Therefore, the aim of this
paper is to review the recent studies on these kinds of phytochemicals in
terms of property and mechanism. Moreover, strategies for improving the
therapeutic efficacy of plant-derived substances such as combination
therapy and nanoformulation based approaches are included.
main objective of this work is to assess the inhibitory effects of silibinin and curcumin, two herbal substances,
on telomerase gene expression in breast cancer cells. Materials and Methods: For determination of cell viability
tetrazolium-based assays were conducted after 24, 48, and 72 h exposure times and expression of human telomerase
reverse transcriptase gene was measured with real-time PCR. Results: Each compound exerted cytotoxic effects
on T47D cells and inhibited telomerase gene expression, both in a time-and dose-dependent manner. The mixture
of curcumin and silibinin showed relatively more inhibitory effect on growth of T47D cells and hTERT gene
expression as compared with either agent alone. Conclusions: These findings suggest that cell viability along
with hTERT gene expression in breast cancer cells could be reduced by curcumin and silibinin.
significant role in synthesizing DNA telomeric in eukaryotes. Telomere
maintenance can cause to immortalization and malignant transformation of
human cells and thereby telomerase activity must be scrutinized as an
important factor in most tumor cells. The proliferation of cancer cells or
apoptosis induction can be suppressed by telomerase inhibition using
different therapeutic agents without any side effects upon normal cells.
Natural substances, with anti-tumor effects, such as those derived from
plants can be suitable candidates due to their capabilities in preventing
some side effects and resistance of tumors with respect to most
chemotherapeutic drugs. In this regards, many studies have shown that
natural phytochemicals have inhibitory effects on telomerase activity
through affecting its subunits and components. Therefore, the aim of this
paper is to review the recent studies on these kinds of phytochemicals in
terms of property and mechanism. Moreover, strategies for improving the
therapeutic efficacy of plant-derived substances such as combination
therapy and nanoformulation based approaches are included.
main objective of this work is to assess the inhibitory effects of silibinin and curcumin, two herbal substances,
on telomerase gene expression in breast cancer cells. Materials and Methods: For determination of cell viability
tetrazolium-based assays were conducted after 24, 48, and 72 h exposure times and expression of human telomerase
reverse transcriptase gene was measured with real-time PCR. Results: Each compound exerted cytotoxic effects
on T47D cells and inhibited telomerase gene expression, both in a time-and dose-dependent manner. The mixture
of curcumin and silibinin showed relatively more inhibitory effect on growth of T47D cells and hTERT gene
expression as compared with either agent alone. Conclusions: These findings suggest that cell viability along
with hTERT gene expression in breast cancer cells could be reduced by curcumin and silibinin.